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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

phB2Mi_i_DsRed2 (#RDB03438)

Expression vector of human microglobulin

Alternative name clone 9
Clone info. Expression vector for human beta 2 microglobulin and DsRed2. PCR fragment of 30 to 394nt was derived from HeLa cell cDNA.
Comment huB2Mi_030F, 5'-ATCGGATCCGAGATGTCTCGCTCCGTG-3'; huB2Mi_394R, 5'-ATCCTCGAGTTACATGTCTCGATCCCACT-3'.
Vector backbone pIRES2-DsRed2 (Plasmid)
Size of vector backbone 5.3 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human beta 2 microglobin cDNA
Depositor|Developer Yokoyama, Kazunari |
 

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Remarks An additional license fee for DNA resources containing fluorescent proteins owned by, or licensed to, Clontech Laboratories, Inc. is required.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB03438 phB2Mi_i_DsRed2 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge plus licensing fee equivalent for fluorescent protein

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The phB2Mi_i_DsRed2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB03438).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB19168_B1F4p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: IRES-R (Pr0037)
Region: IRES2,insert,CMV pro
Sequence file: RDB19168_B1F4a.seq check
>03438_19168_B1F4_3_IRES_R_H01_08_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNTTAG GGGGGGGGGA NGGNNNNGGG CGGATCCCAT
   61 CCTCGAGTTA CATGTCTCGA TCCCACTTAA CTATCTTGGG CTGTGACAAA GTCACATGGT
  121 TCACACGGCA GGCATACTCA TCTTTTTCAG TGGGGGTGAA TTCAGTGTAG TACAAGAGAT
  181 AGAAAGACCA GTCCTTGCTG AAAGACAAGT CTGAATGCTC CACTTTTTCA ATTCTCTCTC
  241 CATTCTTCAG TAAGTCAACT TCAATGTCGG ATGGATGAAA CCCAGACACA TAGCAATTCA
  301 GGAAATTTGA CTTTCCATTC TCTGCTGGAT GACGTGAGTA AACCTGAATC TTTGGAGTAC
  361 GCTGGATAGC CTCCAGGCCA GAAAGAGAGA GTAGCGCGAG CACAGCTAAG GCCACGGAGC
  421 GAGACATCTC GGATCCGATG GGCCCGCGGT ACCGTCGACT GCAGAATTCG AAGCTTGAGC
  481 TCGAGATCTG AGTCCGGTAG CGCTAGCGGA TCTGACGGTT CACTAAACCA GCTCTGCTTA
  541 TATAGACCTC CCACCGTACA CGCCTACCGC CCATTTGCGT CNATGGGGCG GANTTGTTAC
  601 GACNTTTTGG AAAGTCCCNT TGANTTTGNN GNCNAAANAA ACTCCCATTG ACGTCANTGG
  661 GGGNGGANAC TTNNNAANCC CGNGANTCNN NCNNCTATNN NCNNCNNNTN GNNGNNCTNN
721 CNAAAANCNN CATNNNNNNN NGNNNNNNNN NN
//
Primer: IRES-F (Pr0036)
Region: IRES2,DsRed2 5'
Sequence file: RDB19168_B1F4b.seq check
>03438_19168_B1F4_3_IRES_F_G01_07_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNANGNN NNNNAACGTC TAGGCCCCCC GAACCACGGG
   61 GACGTGGTTT TCCTTTGAAA AACACGATGA TAATATGGCC ACAACCATGG CCTCCTCCGA
  121 GAACGTCATC ACCGAGTTCA TGCGCTTCAA GGTGCGCATG GAGGGCACCG TGAACGGCCA
  181 CGAGTTCGAG ATCGAGGGCG AGGGCGAGGG CCGCCCCTAC GAGGGCCACA ACACCGTGAA
  241 GCTGAAGGTG ACCAAGGGCG GCCCCCTGCC CTTCGCCTGG GACATCCTGT CCCCCCAGTT
  301 CCAGTACGGC TCCAAGGTGT ACGTGAAGCA CCCCGCCGAC ATCCCCGACT ACAAGAAGCT
  361 GTCCTTCCCC GAGGGCTTCA AGTGGGAGCG CGTGATGAAC TTCGAGGACG GCGGCGTGGC
  421 GACCGTGACC CAGGACTCCT CCCTGCAGGA CGGCTGCTTC ATCTACAAGG TGAAGTTCAT
  481 CGGCGTGAAC TTCCCCTCCG ACGGCCCCGT GATGCAGAAG AAGACCATGG GCTGGGANGC
  541 CTCCACCGAG CGCCTGTACC CCCGCGACGG CGTGCTGAAN GGCGANACCC ACAAGGCCCT
  601 GAAGCTGAAG GACGGNNGCC ACTACCTGGN GGAGTTNNAG TCTATCTACA TGGNNNAGAA
  661 GCCCGTGCAG CTGCCCNGNT ACTACTACNN NNNNGCNNNG CTGGANNTCN NNTNCCNNNN
721 NNNNGNACNA NNNCNNNNNN NNNN
//
Primer: mRFP1-C_F (Pr0502)
Region: DsRed2 3',SV40 pA,f1 ori
Sequence file: RDB19168_B1F4c.seq check
>03438_19168_B1F4_3mRFP1-C_F_E02_14_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNG GNNNTCACCT CCCACAACGA GGNNTACACC ATCGTGGAGC
   61 AGTACGAGCG CACCGNGGGC CGCCACCACC TGTTCCTGTA GCGGCCGCGA CTCTAGATCA
  121 TAATCAGCCA TACCACATTT GTAGAGGTTT TACTTGCTTT AAAAAACCTC CCACACCTCC
  181 CCCTGAACCT GAAACATAAA ATGAATGCAA TTGTTGTTGT TAACTTGTTT ATTGCAGCTT
  241 ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC
  301 TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTAAGGCGTA AATTGTAAGC
  361 GTTAATATTT TGTTAAAATT CGCGTTAAAT TTTTGTTAAA TCAGCTCATT TTTTAACCAA
  421 TAGGCCGAAA TCGGCAAAAT CCCTTATAAA TCAAAAGAAT AGACCGAGAT AGGGTTGAGT
  481 GTTGTTCCAG TTTGGAACAA GAGTCCACTA TTAAAGAACG TGGACTCCAA CGTCAAAGGG
  541 CGAAAAACCG TCTATCAGGG CGATGGCCCA CTACGTGAAC CATCACCCTA ATCAAGTTTT
  601 TTGGGGTCGA GGTGCCGTAA AGCACTAAAT CGGAACCCTA AANGGAGCCC CCGANTTANA
661 GCNTGACGGG GNAAGCCGGC NNNNGTNGNN ANNAANGNNN NNNNNNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40 pro_ori,NeoR_KanR
Sequence file: RDB19168_B1F4d.seq check
>03438_19168_B1F4_3_SV40pro_ori_F_B01_02_ABI08.ab1
    1 NNNNNNNNNN NNNGCCGCCT CNGCCTCTGA NCTATTCNNG AAGTAGTGAG GAGGCTTTTT
   61 TGGAGGCCTA GGCTTTTGCA AAGATCGATC AAGAGACAGG ATGAGGATCG TTTCGCATGA
  121 TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT
  181 ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC
  241 AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAAG
  301 ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG
  361 ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC
  421 TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC
  481 GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG
  541 AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGANGATCTG GACGAAGAGC
  601 ATCANGGGCT CGCGCCNGCC GAACTGTTCG CCAGGCTCNA GGCGAGCATG CCCNACNGCN
  661 ANGNNCTCGT CGTGNNCCAT NNNNANGNNN GNNNGNNGAA TANCNTNNNN GNAAAATNNN
721 CNNCTTTTCN GNNNNNNNNN N
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles