Resource data sheet
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pUAd5LCALNL(W1975C23R) (#RDB03446)

Shuttle vector to generate recombinant adenovirus

Clone info. Shuttle vector to generate rAd by the two-cosmid rescue method with pAF16Rct (RDB03220) and can also be used to generate fiber-modified rAd by the COS-TPC method.
Comment pUAd5LCALNL is a derivative of pUAd5L (RDB03444) that includes the CALNL cassette [Kanegae et al., Nucleic Acids Res. 23, 3816-21, 1995; Kanegae et al., Gene 181, 207-12, 1996] at the E1 deletion (mu 1.0 to 9.3). It can be used to generate rAd by the two-cosmid rescue method with pAF16Rct (RDB03220) and can also be used to generate fiber-modified rAd by the COS-TPC method [Miyake et al., Proc. Natl. Acad. Sci. USA 93, 1320-24, 1996] without contamination of rAd that expresses wild-type fiber protein. The rAd expresses an encoded gene via the Cre-mediated control of gene expression by an expression cassette that provides the on/off-switching reporter unit and is composed of a CA promoter [Niwa et al., Gene 108, 193-99, 1991.], a loxP sequence, a stuffer sequence, and a second loxP sequence, which is followed by the SwaI cloning site and the G polyA sequence, namely, a CALNL cassette [Kanegae et al., Nucleic Acids Res. 23, 3816-21, 1995; Kanegae et al., Gene 181, 207-12, 1996].
Vector backbone Cosmid, use packaging extracts for transforming E. coli host
Selectable markers Amp^r
Growth conditions 37C, LB+Amp
Gene/insert name AdV_5 whole genome Genomic DNA
Depositor|Developer Yokoyama, Kazunari | Ugai, Hideyo |
 
Remarks, protocol and/or map (pdf) RDB03446.pdf

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution 1. The RECIPIENT agrees to expressly describe the acknowledgement of the Gene Engineering Division, RIKEN BRC as the source of the BIOLOGICAL RESOURCE in any publication.
2. The RECIPIENT shall send a copy of such publication to the Gene Engineering Division, RIKEN BRC.
Remarks Remember that you will be working with samples containing infectious virus.
提供案内 (日本国内) [open/close]

MTAに書く使用条件 リソースを使用して論文を投稿する場合、理研BRC・遺伝子材料開発室から分譲された事を記載する。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Availability Shipping form Fee
RDB03446 pUAd5LCALNL(W1975C23R) Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pUAd5LCALNL(W1975C23R) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB03446).

Reference section:

Ugai H., Murata T., Nagamura Y., Ugawa Y., Suzuki E., Nakata H., Kujime Y., Inamoto S., Hirose M., Inabe K., Terashima M., Yamasaki T., Liu B., Nakade K., Pan J., Kimura M., Saito I., Hamada H., Obata Y., Yokoyama K.K., A database of recombinant viruses and recombinant viral vectors available from the RIKEN DNA bank. J. Gene Med., 7, 1148-1157 (2005). PMID 15945121. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Ugai H., A database of recombinant viruses and recombinant viral vectors available from the RIKEN DNA bank. J. Gene Med., 7, 1148-1157 (2005). PMID 15945121. [PubMed] [Article] [RRC of NBRP]