RDB04026 - pKM2L

Please review the QC test results indicated by check

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pKM2L (#RDB04026)

Reporter construct with Renilla luciferase gene

Alternative name	1142C6F
Clone info.	Plasmid clone of Renilla luciferase reporter vector. The PacI fragment of pEXWS2L (W1005) is cloned into the EcoRV site of pKM2. The MCS1 is able to clone promoter region. The recombinant clone can use for dual-luciferase reporter assay.
Vector backbone	pKM2L (Plasmid)
Selectable markers	Kan^r
Gene/insert name	Renilla reniformis luciferase cDNA
Depositor\|Developer	Yokoyama, Kazunari \|

Sequence	RDB04026z.seq
Additional map
Remarks, protocol and/or map (pdf)	RDB04026.pdf

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Terms and conditions for distribution 1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	謝辞の表明を必要とする。学術研究機関による学術研究にのみ利用できる。 MTAに書く付加的使用条件：本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB04026	pKM2L	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pKM2L was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB04026).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB12398_A4G3p1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 (Pr0410) Region: intron, hRluc Sequence file: RDB12398_A4G3c.seq
>04026_12398_A4G3_M13_(-40)_A01_01.ab1 1 CCGTCGGCAG CTTGCATGCC TGCAGGTCAT TTAAATAGGG ATAACAGGGT AATGATTAAG 61 ATCTCGAGCT CTAAGCTTCA CATATGCATG CACTAGTGGC GCCTGTCGAC GCGTAGAATT 121 CACCCGGGTA CTGCAGAAGT TGGTCGTGAG GCACTGGGCA GGTAAGTATC AAGGTTACAA 181 GACAGGTTTA AGGAGACCAA TAGAAACTGG GCTTGTCGAG ACAGAGAAGA CTCTTGCGTT 241 TCTGATAGGC ACCTATTGGT CTTACTGACA TCCACTTTGC CTTTCTCTCC ACAGGTGTCC 301 ACTCCCAGTT CAATTACAGC TCTTAAGGCT AGAGTACTTA ATACGACTCA CTATAGGCTA 361 GCCACCATGG CTTCCAAGGT GTACGACCCC GAGCAACGCA AACGCATGAT CACTGGGCCT 421 CAGTGGTGGG CTCGCTGCAA GCAAATGAAC GTGCTGGACT CCTTCATCAA CTACTATGAT 481 TCCGAGAAGC ACGCCGAGAA CGCCGTGATT TTTCTGCATG GTAACGCTGC CTCCAGCTAC 541 CTGTGGAGGC ACGTCGTGCC TCACATCGAG CCCGTGGCTA GATGCATCAT CCCTGATCTG 601 ATCGGAATGG GTAAGTCCGG CAAGAGCGGG AATGGCTCAT ATCGCCTCCT GGATCACTAC 661 AAGTACCTCA CCGCTTGGTT CGAGCTGCTG AACCTTCCAA AGAAAATCAT CTTTGTGGGC 721 CACGACTGGG GGGCTTGTCT GGCCTTTCAC TACTCCTACG AGCACCAAGA CAAGATCAAG 781 GCCATCGTCC ATGCTGAGAG TGTCGTGGAC GTGATCGAGT CCTGGGACGA GTGGCCTGAC 841 ATCGAGGAGG ATATCGCCCT GATCAAGAGC GAAGAGGGCG AGAAAATGGT GCTTGAGAAT 901 AACTTCTTCG TCGAGACCAT GCTCCCAAGC AAGATCATGC GGAAACTGGA GCCTGAGGAG 961 TTCGCTGCCT ACCCTGGAGC CATTCAAGGG AGAAAGGCGA GGTTAGACGG GCCTACCCTC 1021 TCCTGGTCCT CGCGAGGATC CCTCTCGTTA GGAGGCCAGG CCCGACGTTC GTCAGATTGA 1081 TCGCGCTACC ACGACTAACC CTTCGGACCA GGCGAACGA //
Primer: pAxCALNLF1 (Pr0166) Region: SV40 p(A), hRluc Sequence file: RDB12398_A4G3d.seq
>04026_12398_A4G3_pAxCALNLF1_B01_04.ab1 1 ACCATTTATC TTATCATGTC TGCTCGAAGC GGCCGCTCTA GAATTACTGC TCGTTCTTCA 61 GCACGCGCTC CACGAAGCTC TTGATGTACT TACCCATTTC ATCTGGAGCG TCCTCCTGGC 121 TGAAGTGGAG GCCCTTCACC TTCACGAACT CGGTGTTAGG GAACTTCTTA GCTCCCTCGA 181 CAATAGCGTT GGAAAAGAAC CCAGGGTCGG ACTCGATGAA CATCTTAGGC AGATCGTCGC 241 TGGCCCGAAG GTAGGCGTTG TAGTTGCGGA CAATCTGGAC GACGTCGGGC TTGCCTCCCT 301 TAACGAGAGG GATCTCGCGA GGCCAGGAGA GGGTAGGCCG TCTAACCTCG CCCTTCTCCT 361 TGAATGGCTC CAGGTAGGCA GCGAACTCCT CAGGCTCCAG TTTCCGCATG ATCTTGCTTG 421 GGAGCATGGT CTCGACGAAG AAGTTATTCT CAAGCACCAT TTTCTCGCCC TCTTCGCTCT 481 TGATCAGGGC GATATCCTCC TCGATGTCAG GCCACTCGTC CCAGGACTCG ATCACGTCCA 541 CGACACTCTC AGCATGGACG ATGGCCTTGA TCTTGTCTTG GTGCTCGTAG GAGTAGTGAA 601 AGGCCAGACA AGCCCCCCAG TCGTGGCCCA CAAAGATGAT TTTCTTTGGA AGGTTCAGCA 661 GCTCGAACCA AGCGGTGAGG TACTTGTAGT GATCCAGGAG GCGATATGAG CCATTCCCGC 721 TCTTGCCGGA CTTACCCATT CCGATCAGAT CAGGGATGAT GCATCTAGCC ACGGGCTCGA 781 TGTGAGGCAC GACGTGCCTC CACAGGTAGC TGGAGGCAGC GTTACCATGC AGAAAAATCA 841 CGGCGTTCTC GGCGTGCTTC TCGGAATCAT AGTAGTTGAT GAAGGAGTCC AGCACGTTCA 901 TTTGCTTGCA GCGAGCCCAC CACTGAGGCC CAGTGATCAT GCGTTTGCGT TGCTCGGGGG 961 TCGTACACCT TGGAAAGCCA TGGTGGCTAG CCTATAGTGA GTCGTATTTA AGTACTCTAG 1021 CCCTTTAGAG CTGTAATTTG AACTGGAAGT TGACACCTGT TGGAGAGAAG CAAGTGATGG 1081 TCAGGTTAGA CCAATAGGGT GGCCCTATTC CAGGA //