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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pKM2L

Reporter construct with Renilla luciferase gene

Catalog number RDB04026
Resource name pKM2L
Alternative name 1142C6F
Clone info. Plasmid clone of Renilla luciferase reporter vector. The PacI fragment of pEXWS2L (W1005) is cloned into the EcoRV site of pKM2. The MCS1 is able to clone promoter region. The recombinant clone can use for dual-luciferase reporter assay.
Vector backbone pKM2L (Plasmid)
Selectable markers Kan^r
Gene/insert name Renilla reniformis luciferase cDNA
Depositor|Developer Yokoyama, Kazunari |
Other clones in our bank

External Database
Renilla reniformis luciferase

            Reference sequence
              
             
            Sequence RDB04026z.seq
            Remarks, protocol and/or map (gif) RDB04026a.gif
            Remarks, protocol and/or map (pdf) RDB04026.pdf

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution 1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
            Additional terms and conditions:
            The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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            RDB04026 pKM2L DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Original reference

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Promoter Collection (Outline) (English text)
            Featured content Promoter Collection (Outline) (Japanese text)
            Featured content Promoter Collection (Renilla Luciferase) (English text)
            Featured content Arranged vector (English text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB12398_A4G3p1-2.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: M13_-40
            Region: intron, hRluc
            Sequence file: RDB12398_A4G3c.seq check
            >04026_12398_A4G3_M13_(-40)_A01_01.ab1
                1 CCGTCGGCAG CTTGCATGCC TGCAGGTCAT TTAAATAGGG ATAACAGGGT AATGATTAAG
               61 ATCTCGAGCT CTAAGCTTCA CATATGCATG CACTAGTGGC GCCTGTCGAC GCGTAGAATT
              121 CACCCGGGTA CTGCAGAAGT TGGTCGTGAG GCACTGGGCA GGTAAGTATC AAGGTTACAA
              181 GACAGGTTTA AGGAGACCAA TAGAAACTGG GCTTGTCGAG ACAGAGAAGA CTCTTGCGTT
              241 TCTGATAGGC ACCTATTGGT CTTACTGACA TCCACTTTGC CTTTCTCTCC ACAGGTGTCC
              301 ACTCCCAGTT CAATTACAGC TCTTAAGGCT AGAGTACTTA ATACGACTCA CTATAGGCTA
              361 GCCACCATGG CTTCCAAGGT GTACGACCCC GAGCAACGCA AACGCATGAT CACTGGGCCT
              421 CAGTGGTGGG CTCGCTGCAA GCAAATGAAC GTGCTGGACT CCTTCATCAA CTACTATGAT
              481 TCCGAGAAGC ACGCCGAGAA CGCCGTGATT TTTCTGCATG GTAACGCTGC CTCCAGCTAC
              541 CTGTGGAGGC ACGTCGTGCC TCACATCGAG CCCGTGGCTA GATGCATCAT CCCTGATCTG
              601 ATCGGAATGG GTAAGTCCGG CAAGAGCGGG AATGGCTCAT ATCGCCTCCT GGATCACTAC
              661 AAGTACCTCA CCGCTTGGTT CGAGCTGCTG AACCTTCCAA AGAAAATCAT CTTTGTGGGC
              721 CACGACTGGG GGGCTTGTCT GGCCTTTCAC TACTCCTACG AGCACCAAGA CAAGATCAAG
              781 GCCATCGTCC ATGCTGAGAG TGTCGTGGAC GTGATCGAGT CCTGGGACGA GTGGCCTGAC
              841 ATCGAGGAGG ATATCGCCCT GATCAAGAGC GAAGAGGGCG AGAAAATGGT GCTTGAGAAT
              901 AACTTCTTCG TCGAGACCAT GCTCCCAAGC AAGATCATGC GGAAACTGGA GCCTGAGGAG
              961 TTCGCTGCCT ACCCTGGAGC CATTCAAGGG AGAAAGGCGA GGTTAGACGG GCCTACCCTC
             1021 TCCTGGTCCT CGCGAGGATC CCTCTCGTTA GGAGGCCAGG CCCGACGTTC GTCAGATTGA
            1081 TCGCGCTACC ACGACTAACC CTTCGGACCA GGCGAACGA
            //
            Primer: pAxCALNLF1
            Region: SV40 p(A), hRluc
            Sequence file: RDB12398_A4G3d.seq check
            >04026_12398_A4G3_pAxCALNLF1_B01_04.ab1
                1 ACCATTTATC TTATCATGTC TGCTCGAAGC GGCCGCTCTA GAATTACTGC TCGTTCTTCA
               61 GCACGCGCTC CACGAAGCTC TTGATGTACT TACCCATTTC ATCTGGAGCG TCCTCCTGGC
              121 TGAAGTGGAG GCCCTTCACC TTCACGAACT CGGTGTTAGG GAACTTCTTA GCTCCCTCGA
              181 CAATAGCGTT GGAAAAGAAC CCAGGGTCGG ACTCGATGAA CATCTTAGGC AGATCGTCGC
              241 TGGCCCGAAG GTAGGCGTTG TAGTTGCGGA CAATCTGGAC GACGTCGGGC TTGCCTCCCT
              301 TAACGAGAGG GATCTCGCGA GGCCAGGAGA GGGTAGGCCG TCTAACCTCG CCCTTCTCCT
              361 TGAATGGCTC CAGGTAGGCA GCGAACTCCT CAGGCTCCAG TTTCCGCATG ATCTTGCTTG
              421 GGAGCATGGT CTCGACGAAG AAGTTATTCT CAAGCACCAT TTTCTCGCCC TCTTCGCTCT
              481 TGATCAGGGC GATATCCTCC TCGATGTCAG GCCACTCGTC CCAGGACTCG ATCACGTCCA
              541 CGACACTCTC AGCATGGACG ATGGCCTTGA TCTTGTCTTG GTGCTCGTAG GAGTAGTGAA
              601 AGGCCAGACA AGCCCCCCAG TCGTGGCCCA CAAAGATGAT TTTCTTTGGA AGGTTCAGCA
              661 GCTCGAACCA AGCGGTGAGG TACTTGTAGT GATCCAGGAG GCGATATGAG CCATTCCCGC
              721 TCTTGCCGGA CTTACCCATT CCGATCAGAT CAGGGATGAT GCATCTAGCC ACGGGCTCGA
              781 TGTGAGGCAC GACGTGCCTC CACAGGTAGC TGGAGGCAGC GTTACCATGC AGAAAAATCA
              841 CGGCGTTCTC GGCGTGCTTC TCGGAATCAT AGTAGTTGAT GAAGGAGTCC AGCACGTTCA
              901 TTTGCTTGCA GCGAGCCCAC CACTGAGGCC CAGTGATCAT GCGTTTGCGT TGCTCGGGGG
              961 TCGTACACCT TGGAAAGCCA TGGTGGCTAG CCTATAGTGA GTCGTATTTA AGTACTCTAG
             1021 CCCTTTAGAG CTGTAATTTG AACTGGAAGT TGACACCTGT TGGAGAGAAG CAAGTGATGG
            1081 TCAGGTTAGA CCAATAGGGT GGCCCTATTC CAGGA
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles


            2022.10.11

            GNP_filter3_RDBDEP_html_221010.pl