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pKM2L (#RDB04026)

Reporter construct with Renilla luciferase gene


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Unpublished bioresource
Test sheet Data Sheet open 
 
Alternative name 1142C6F
Clone info. Plasmid clone of Renilla luciferase reporter vector. The PacI fragment of pEXWS2L (W1005) is cloned into the EcoRV site of pKM2. The MCS1 is able to clone promoter region. The recombinant clone can use for dual-luciferase reporter assay.
Vector backbone pKM2L (Plasmid)
Selectable markers Kan^r
Growth conditions 37℃, LB+Kan
Gene/insert name Renilla reniformis luciferase cDNA
Depositor|Developer Yokoyama, Kazunari |

Distribution information

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Terms and conditions for distribution 1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
提供案内 (日本国内) [open/close]

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付加的使用条件：
本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

Catalog # Resource name Shipping form Fee
RDB04026 pKM2L DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pKM2L was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB04026).

Reference section:

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

user_report Kim, K.L., SCF (Stem Cell Factor) and cKIT Modulate Pathological Ocular Neovascularization. Arterioscler. Thromb. Vasc. Biol. 39 (10): 2120-2131 (2019). PMID 31434494. [PubMed] [Article] [RRC of NBRP]