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CEPH MEGA YAC clone (#RDB04215)

CEPH MEGA YAC clone

Clone info. CEPH MEGA YAC clone originally established by Foundation Jean-Dausset Council on Education for Public Health (CEPH).
Vector backbone pYAC4 ()
Size of vector backbone 11.5 kb
Selectable markers URA3, TRP1, sup4
Growth remarks It is recommended that colonies be isolated and cultured on an appropriate agar plate before performing your experiments.
Gene/insert name Human anonymous gene Genomic DNA
Depositor|Developer CEPH, |
 

Distribution information

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Please visit CEPH MEGA YAC clone.[link] 
Terms and conditions for distribution (1) The YAC library was originally established by Foundation Jean-Dausset Council on Education for Public Health (CEPH). Therefore, the clones must be reported as CEPH YAC clones.
(2) The original names of the YAC clones must be used in all relevant research and in all relevant publications. Renaming of the clones is prohibited.
(3) At publication, recipients should acknowledge that YAC clones were derived from the CEPH YAC library and should acknowledge and the RIKEN DNA Bank for screening of clones. Two copies of the published paper should be sent to the CEPH and the RIKEN BRC.
(4) In addition to publications, all screening data must be sent to CEPH when the probes used are in the public domain.
Remarks It is recommended that colonies be isolated and cultured on an appropriate agar plate before performing your experiments.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
手続きの概要は、「CEPH MEGA YAC clone[link]」をご覧ください。
MTAに書く使用条件 (1) The YAC library was originally established by Foundation Jean-Dausset Council on Education for Public Health (CEPH). Therefore, the clones must be reported as CEPH YAC clones.
(2) The original names of the YAC clones must be used in all relevant research and in all relevant publications. Renaming of the clones is prohibited.
(3) At publication, recipients should acknowledge that YAC clones were derived from the CEPH YAC library and should acknowledge and the RIKEN DNA Bank for screening of clones. Two copies of the published paper should be sent to the CEPH and the RIKEN BRC.
(4) In addition to publications, all screening data must be sent to CEPH when the probes used are in the public domain.
備考 提供条件の言語は英語のみ。実験を行う前に、適切な寒天プレート上でコロニーを単離し、培養することを推奨します。
組換え体提供にかかる書式が必要です。

References

Original, user report and related articles

original Cohen, D., A first-generation physical map of the human genome. Nature, 366, 698-701 (1993). PMID 8259213. [link to RRC of NBRP]
original Chumakov, I., Continuum of overlapping clones spanning the entire human chromosome 21q. Nature, 359, 380-386 (1992). PMID 1406950. [link to RRC of NBRP]
reference Li, L., Generation of transgenic mice with megabase-sized human yeast artificial chromosomes by yeast spheroplast-embryonic stem cell fusion. Nat. Protoc., 8 (8): 1567-1582 (2013). PMID 23868074.
reference Burke, D.T., Cloning of large segments of exogenous DNA into yeast by means of artificial chromosome vectors. Science 236 (4803): 806-812 (1987). PMID 3033825.
user_report Suzuki T., Mutation analyses of genes on 6p12-p11 in patients with juvenile myoclonic epilepsy. Neurosci. Lett., 405, 126-131 (2006). PMID 16876319. [link to RRC of NBRP]
user_report Yagasaki F., Fusion of TEL/ETV6 to a novel ACS2 in myelodysplastic syndrome and acute myelogenous leukemia with t(5;12)(q31;p13). Gene. Chromosome. Canc., 26, 192-202 (1999). PMID 10502316. [link to RRC of NBRP]