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pGEX-G3-1a (#RDB04219)

Bacteria expression vector of human galectin-3, GST fusion protein

Clone info. Expression vector of human galectin-3.
Comment Expression was confirmded by the depositor by SDS-PAGE followed by CBB staining.
Vector backbone pGEX-4T-2 (Plasmid)
Size of vector backbone 5.0 kb
Selectable markers Amp^r
Gene/insert name Human galectin-3 cDNA
Depositor|Developer Nishi, Nozomu |
 
Remarks, protocol and/or map (pdf) RDB04219.pdf

Distribution information

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Terms and conditions for distribution With no restriction.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
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MTAに書く使用条件 条件を付加しない。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB04219 pGEX-G3-1a DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGEX-G3-1a was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB04219).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13382_A5Ft.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGEX-F
Sequence file: RDB13382_A5Fta.seq check
>04219_13382_A5Ft_A_pGEX-F_A01_01.ab1
    1 GGCATCGTCA ATCGGATCTG GTTCCGCGTG GATCCCCAGG AATTCCCATG GCAGACAATT
   61 TTTCGCTCCA TGATGCGTTA TCTGGGTCTG GAAACCCAAA CCCTCAAGGA TGGCCTGGCG
  121 CATGGGGGAA CCAGCCTGCT GGGGCAGGGG GCTACCCAGG GGCTTCCTAT CCTGGGGCCT
  181 ACCCCGGGCA GGCACCCCCA GGGGCTTATC CTGGACAGGC ACCTCCAGGC GCCTACCATG
  241 GAGCACCTGG AGCTTATCCC GGAGCACCTG CACCTGGAGT CTACCCAGGG CCACCCAGCG
  301 GCCCTGGGGC CTACCCATCT TCTGGACAGC CAAGTGCCCC CGGAGCCTAC CCTGCCACTG
  361 GCCCCTATGG CGCCCCTGCT GGGCCACTGA TTGTGCCTTA TAACCTGCCT TTGCCTGGGG
  421 GAGTGGTGCC TCGCATGCTG ATAACAATTC TGGGCACGGT GAAGCCCAAT GCAAACAGAA
  481 TTGCTTTAGA TTTCCAAAGA GGGAATGATG TTGCCTTCCA CTTTAACCCA CGCTTCAATG
  541 AGAACAACAG GAGAGTCATT GTTTGCAATA CAAAGCTGGA TAATAACTGG GGAAGGGAAG
  601 AAAGACAGTC GGTTTTCCCA TTTGAAAGTG GGAAACCATT CAAAATACAA GTACTGGTTG
  661 AACCTGACCA CTTCAAGGTT GCAGTGAATG ATGCTCACTT GTTGCAGTAC AATCATCGGG
  721 TTAAAAAACT CAATGAAATC AGCAAACTGG GAATTTCTGG TGACATAGAC CTCACCAGTG
  781 CTTCATATAC CATGATATAA CTCGAGCGGC CGCATCGTGA CTGACTGACG ATCTGCCTCG
  841 CGCGTTTCGG TGATGACGGT GAAAACCTCT GACACATGCA GCTCCCGGAG ACGGTCACAG
  901 CTTGTCTGTA AGCGGATGCC GGGAGCAGAC AAGCCCGTCA GGGCGCGTCA GCGGGTGTTG
  961 GCGGGTGTCG GGGCGCAGCC ATGACCCAGT CACGTAGCGA TAGCGGGAGT GTATAATTCT
 1021 TGAAGACGAA AGGCCTCGTG ATACGCCTAT TTTTTTATAG GTTATGTCAT GATAATAATG
 1081 GACTTTTCTT AGACGGTCAA GCTGCACTTT TCTGGCAATG TGCGCGGCAA TCCTACTGAT
1141 TAATTTTTCG TAAAT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

protocol Nishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). [link to RRC of NBRP]