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pTrc-G4-1a (#RDB04220)

Bacteria expression vector of human galectin-4, (His) 6 -containing-tag protein

Clone info. Expression vector of human galectin-4.
Vector backbone pTrcHis B (Plasmid)
Size of vector backbone 4.4 kb
Selectable markers Amp^r
Gene/insert name Human lectin, galactoside-binding, soluble, 4 cDNA
Depositor|Developer Nishi, Nozomu |
 
Remarks, protocol and/or map (pdf) RDB04220.pdf

Distribution information

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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB04220 pTrc-G4-1a DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pTrc-G4-1a was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB04220).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13755_A6A4p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: Reverse2
Sequence file: RDB13755_A6A4a.seq check
>04220_13755_A6A4_Reverse2_E08_14.ab1
    1 CCCCGTGAAA GCGAGCGGCA CTGCTCTTTA CAATTTATCA GACAATCTGT GTGGGCACTC
   61 GACCGGAATT ATCGATTAAC TTTATTATTA AAAATTAAAG AGGTATATAT TAATGTATCG
  121 ATTAAATAAG GAGGAATAAA CCATGGGGGG TTCTCATCAT CATCATCATC ATGGTATGGC
  181 TAGCATGACT GGTGGACAGC AAATGGGTCG GGATCTGTAC GACGATGACG ATAAGGATCC
  241 GAGCTCGAGA ATGGCCTATG TCCCCGCACC GGGCTACCAG CCCACCTACA ACCCGACGCT
  301 GCCTTACTAC CAGCCCATCC CGGGCGGGCT CAACGTGGGA ATGTCTGTTT ACATCCAAGG
  361 AGTGGCCAGC GAGCACATGA AGCGGTTCTT CGTGAACTTT GTGGTTGGGC AGGATCCGGG
  421 CTCAGACGTC GCCTTCCACT TCAATCCGCG GTTTGACGGC TGGGACAAGG TGGTCTTCAA
  481 CACGTTGCAG GGCGGGAAGT GGGGCAGCGA GGAGAGGAAG AGGAGCATGC CCTTCAAAAA
  541 GGGTGCCGCC TTTGAGCTGG TCTTCATAGT CCTGGCTGAG CACTACAAGG TGGTGGTAAA
  601 TGGAAATCCC TTCTATGAGT ACGGGCACCG GCTTCCCCTA CAGATGGTCA CCCACCTGCA
  661 AGTGGATGGG GATCTGCAAC TTCAATCAAT CAACTTCATC GGAGGCCAGC CCCTCCGGCC
  721 CCAGGGACCC CCGATGATGC CACCTTACCC TGGTCCCGGA CATTGCCATC AACAGCTGAA
  781 CAGCCTGCCC ACCATGGAAG GACCCCCCAA CCTTCAACCC GCCTGTGCCA TATTTCGGGA
  841 GGCTGCAAAG GAGGGCTCAC AGCTCGAAAG AACCCATCAT CATCAAAGGC TATGTGCCCT
  901 CCCACAGGCA AGAGCTTTGC TATCAACTTC AAAGTGGGCT CCTCACGGGA CATAGCTCTG
  961 CACATTAATC CCCGCATGGG CACGGGTACC GTTGGCTCAG ACCAGTCGTT CCTGAATGGC
1021 TTCGTTGGGA ATCGAAGAGA GAAGATTCAG CGACACCCAT TTTGGATTTC
//
Primer: pTrcHis-R
Sequence file: RDB13755_A6A4b.seq check
>04220_13755_A6A4_pTrcHis-R_F08_17.ab1
    1 GGGCCGGCCG TATCTTCTCT CATCCGCCAA CAGCCAAGCT TCGAATTCTT AGATCTGGAC
   61 ATAGGACAAG GTGACATCAC CCTGGATTTC CAATGTGTCC ACCCTCTGGA AGGCCGAGAG
  121 GCGATGGGCA AAGTCAAAGA GGTGCTGGCC ATTGGCGTAA ACCTTGAAGC GATCCAAGCC
  181 ACAGCGAATG GACAGATCAA AGAACTGTCC GGGACCAAAT GGGTTGTGGG TGATCTTCTT
  241 CTCCTCGGAT CCCCACGAGC CATTCAGAAG GCTGTTCCGG ACCACGGTAC CGTTGCCCAT
  301 GCGGGGATTA ATGTGCAGAG CTATGTCCCC TGAGGAGCCC ACCTTGAAGT TGATAGCAAA
  361 GCTCTTGCCT GTGGGAGGCA CATAGCCCTT GATGATGATG GTTCTTCGAG CTGTGAGCCC
  421 TCCTTGCAGC CTCCCGAAAT ATGGCACAGG CGGGTTGAAG GTTGGGGGTC CTTCCATGGT
  481 GGGCAGGCTG TTCAGCTGTT GATGGCAATG TCCGGGACCA GGGTAAGGTG GCATCATCGG
  541 GGGTCCCTGG GGCCGGAGGG GCTGGCCTCC GATGAAGTTG ATTGATTGAA GTTGCAGATC
  601 CCCATCCACT TGCAGGTGGG TGACCATCTG TAGGGGAAGC CGGTGCCCGT ACTCATAGAA
  661 GGGATTTCCA TTTACCACCA CCTTGTAGTG CTCAGCCAGG ACTATGAAGA CCAGCTCAAA
  721 GGCGGCACCC TTTTTGAAGG GCATGCTCCT CTTCCTCTCC TCGCTGCCCC ACTTCCCGCC
  781 CTGCAACGTG TTGAAGACCA CCTTGTCCCA GCCGTCAAAC CGCGGATTGA AGTGGAAGGC
  841 GACGTCTGAG CCCGGATCCT GCCCAACCAC AAAGTTCACG AAAGAACCGC TTCATGTGCT
  901 CGCTGGCCAC TCCCTTGGAT GTAAACAGAC ATTTCCCACG TTTGAGCCCG CCCGGGATGG
  961 GCTGGTAGTA AAGCAGCGTC CGGATGTAGG TGGGCTGGTA GCCCGGTGCG GGGACATAGC
 1021 CCATTCTCGA GCTTCGAATT CGTATCGTTC ATCGATCGTA CAGGATTCCC GATCGATTAG
1081 CTGTCCACCA GTTCAAT
//
Primer: pGEX-R
Sequence file: RDB13755_A6A4c.seq check
>04220_13755_A6A4_pGEX-R_G08_20.ab1
    1 TTGGATTAGG ACGCGCGAGC AGCAGATCAA TTCGCGCGCG AAGGCGAAGC GGCATGCATT
   61 TACGTTGACA CCATCGAATG GTGCAAAACC TTTCGCGGTA TGGCATGATA GCGCCCGGAA
  121 GAGAGTCAAT TCAGGGTGGT GAATGTGAAA CCAGTAACGT TATACGATGT CGCAGAGTAT
  181 GCCGGTGTCT CTTATCAGAC CGTTTCCCGC GTGGTGAACC AGGCCAGCCA CGTTTCTGCG
  241 AAAACGCGGG AAAAAGTGGA AGCGGCGATG GCGGAGCTGA ATTACATTCC CAACCGCGTG
  301 GCACAACAAC TGGCGGGCAA ACAGTCGTTG CTGATTGGCG TTGCCACCTC CAGTCTGGCC
  361 CTGCACGCGC CGTCGCAAAT TGTCGCGGCG ATTAAATCTC GCGCCGATCA ACTGGGTGCC
  421 AGCGTGGTGG TGTCGATGGT AGAACGAAGC GGCGTCGAAG CCTGTAAAGC GGCGGTGCAC
  481 AATCTTCTCG CGCAACGCGT CAGTGGGCTG ATCATTAACT ATCCGCTGGA TGACCAGGAT
  541 GCCATTGCTG TGGAAGCTGC CTGCACTAAT GTTCCGGCGT TATTTCTTGA TGTCTCTGAC
  601 CAGACACCCA TCAACAGTAT TATTTTCTCC CATGAAGACG GTACGCGACT GGGCGTGGAG
  661 CATCTGGTCG CATTGGGTCA CCAGCAAATC GCGCTGTTAG CGGGCCCATT AAGTTCTGTC
  721 TCGGCGCGTC TGCGTCTGGC TGGCTGGCAT AAATATCTCA CTCGCAATCA AATTCAGCCG
  781 ATAGCGGAAC GGGAAGGCGA CTGGAGTGCC ATGTCCGGTT TTCAACAAAC CATGCAAATG
  841 CTGAATGAGG GCATCGTTCC CACTGCGATG CTGGTTGCCA ACGATCAGAT GGCGCTGGGC
  901 GCAATGCGCG CCATTACCGA GTCCCGGGCT GCGCGTTTGG GTGCGGATAT CTCGGGTAGT
  961 GGATACGACG ATACCCGAGA CAGCTCATGT ATATCCCGGC CGGTAACAAC AATCAACAGA
1021 TTTTCGCTTG CCTGGGCAAC CAGCGATGAC CGCTTGGCCT GCCACTCCTT CTCCA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

protocol Nishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). [link to RRC of NBRP]