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pGEX-G9(M)-1a (#RDB04223)

Bacteria expression vector of human galectin-9 (the predominant isoform of G9 in most cell and tissue types, G9(M)/G9M), GST fusion protein


Drawn by SnapGene® software
Sequence (full) RDB04223hts01.seq
Remarks, protocol and/or map (pdf) RDB04223.pdf
Publication Matsushita, N., J. Biol. Chem., 275, 8355-8360 (2000). [link to RRC of NBRP]
Test sheet RDB19857_B2Lpp1-1.pdf 
Assembled from experimentally sequenced data.
 
Clone info. Expression vector of galectin-9.
Comment Expression was confirmded by the depositor by SDS-PAGE followed by CBB staining.
Vector backbone pGEX-4T-2 (Plasmid)
Size of vector backbone 5.0 kb
Selectable markers Amp^r
Gene/insert name Human lectin, galactoside-binding, soluble, 9 cDNA
Depositor|Developer Nishi, Nozomu |

Distribution information

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Terms and conditions for distribution With no restriction.
提供案内 (日本国内) [open/close]

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RDB04223 pGEX-G9(M)-1a DNA solution Please contact us.

How to cite this biological resource

Materials & Methods section:

The pGEX-G9(M)-1a was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB04223).

Reference section:

Matsushita, N., Nishi, N., Seki, M., Matsumoto, R., Kuwabara, I., Liu, F.T., Hata, Y., Nakamura, T., Hirashima, M., Requirement of divalent galactoside-binding activity of ecalectin/galectin-9 for eosinophil chemoattraction. J. Biol. Chem., 275, 8355-8360 (2000). PMID 10722666. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Matsushita, N., Requirement of divalent galactoside-binding activity of ecalectin/galectin-9 for eosinophil chemoattraction. J. Biol. Chem., 275, 8355-8360 (2000). PMID 10722666. [link to RRC of NBRP]
protocol Nishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). [link to RRC of NBRP]