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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pGEX-LNG12-3 (#RDB04225)

Bacteria expression vector of human galectin-12 with an N-terminally extended NCRD (LNG12), GST fusion protein

Clone info. Expression vector of human galectin-12.
Vector backbone pGEX-4T-2 (Plasmid)
Size of vector backbone 5.0 kb
Selectable markers Amp^r
Gene/insert name Human lectin, galactoside-binding, soluble, 12 cDNA
Depositor|Developer Nishi, Nozomu |
 
Remarks, protocol and/or map (pdf) RDB04225.pdf

Distribution information

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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB04225 pGEX-LNG12-3 DNA solution Please contact us.

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGEX-LNG12-3 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB04225).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15792_A8Bep1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGEX-F2
Sequence file: RDB15792_A8Bea.seq check
>04225_15792_A8Be_pGEX-F2_B02_05_ABI24.ab1
    1 CATATTAAGT TTTGGTTTTT AAAAACGTAT TGAAGCTATC CCACAAATTG ATAAGTACTT
   61 GAAATCCAGC AAGTATATAG CATGGCCTTT GCAGGGCTGG CAAGCCACGT TTGGTGGTGG
  121 CGACCATCCT CCAAAATCGG ATCTGGTTCC GCGTGGATCC ATGAGTCAGC CCAGTGGGGG
  181 CAGGGCTCCT GGAACGAGGA TCTACAGTTG GAGTTGCCCC ACTGTCATGT CACCTGGAGA
  241 AAAACTGGAC CCAATTCCTG ACAGCTTCAT TCTGCAACCA CCAGTCTTCC ACCCGGTGGT
  301 TCCTTATGTC ACGACGATTT TTGGAGGCCT GCATGCAGGC AAGATGGTCA TGCTGCAAGG
  361 AGTGGTCCCT CTAGATGCAC ACAGGTTTCA GGTGGACTTC CAGTGTGGCT GCAGCCTGTG
  421 TCCCCGGCCA GATATCGCCT TCCACTTCAA CCCTCGCTTC CATACCACCA AGCCCCATGT
  481 CATCTGCAAC ACCCTGCATG GTGGACGCTG GCAAAGGGAG GCCCGGTGGC CCCACCTGGC
  541 CCTGCGAAGA GGCTCCAGCT TCCTCATCCT CTTTCTCTTC GGGAATGAGG AAGTGAAGGT
  601 GAGTGTGAAT GGACAGCACT TTCTCCACTT CCGCTACCGG CTCCCACTGT CTCATGTGGA
  661 CACGCTGGGT ATATTTGGTG ACATCCTGGT AGAGGCTGTT GGATTCCTGA ACATCAATCC
  721 ATTTGTGGAG GGCAGCAGAG AGTACCCAGC TGGACATCCT TTCCTGCTGA TGAGCCCCAG
  781 GCTGGAGGTG CCCTGCTCAC ATGCTCTTCC CCAGGGTCTC TCGCCTGGGG CAGGTCATCA
  841 TAGTACGGGG ACTGGTCTTG CAAGAGCCGA AGCATTTTAC TGTGAGCCTG AGGGGACCAG
  901 GCTGCCCATG CTCCTGTGAC ACTCAGGGCC TCCTTCGCAG ACAGAACTCT GGGCCTGGAT
  961 CTCCCGCTGG GGGGCAGAAG AAACTGATCT CAGCCCCCTT TCCTCTTTTA CCCCCAGAGA
 1021 TTCTTTTGGA GGTGGCTGCT CCTGGTTCCA GGAGGGAGGG CTGAAGGCTG GGCGCTCAAT
 1081 GGGGCAGGGG CTGGGGGGGC ATCAGCATGG AACAGCAGTC TTGGAGCAGC TGGCGGGGAG
1141 CTTCGGAATC AGTGGGAAGG GTGTCAGAC
//
Primer: pGEX-RSEQ
Sequence file: RDB15792_A8Beb.seq check
>04225_15792_A8Be_pGEX-RSEQ_C02_08_ABI24.ab1
    1 ACACAAAACG GAAACGGCGC GAGGCAGATC GTCAGTCAGT CACGATGCGG CCGCTCGAGT
   61 CGACCCGGGA ATTCTCAGGA GTGGACACAG TAGAGCTGGA CACTTCCACT GATCCGGAGC
  121 TCCCGCAGCT GCTCCAGGGC CTGCTGGTTC ATGCTGGTGG CCCCCAGCCC CTGCCCATTG
  181 AGCGCCAGCT TCAGCCCTCC CTCCTGGAAC AGGAGCAGCA CCTCAAAGAA TCTCTGGGGG
  241 TAAAAGAGGA AGGGGGCTGA GATCAGTTTC TTCTGCCCCC AGCGGGAGAT CCAGGCCAGA
  301 GTTCTGTCTG CGAAGGAGGC CCTGAGTGTC ACAGGAGCAT GGGCAGCCTG GTCCCTCAGG
  361 CTCACAGTAA AATGCTTCGG CTCTTGCAAG ACCAGTCCCC GTACTATGAT GACCTGCCCA
  421 GGCGAGAGAC CCTGGGGAAG AGCATGTGAC AGGGCACCTC CAGCCTGGGG CTCATCACAG
  481 AAAGGATGTC CAGCTGGGAC TCTCTGCTGC CCTCCCAATG GATTGTGGTC AGGATCCAAC
  541 GCCTCTACCA GAGTCACCAA TATACCCGCG TGTCACATAA AAATGGAACC GGTACGAAGT
  601 GAAAAGGGTG GCATTCAATC ACTTAATCCT CATCCGAAAA AAAAGAAAAA AAATGAGCTC
  661 TCCAGGCCAG GGGCACGGCT CCTGCCGTAC ATGGGGTTCA TAATGGCTGG ATGACAGTTA
  721 ATAAGAATGG CGGACAGTTC CCATGAACCT AGACTGGATT AAGGACCTTA ATAACTTGCG
  781 AGAGCTTAAT TTGGGATGGA CCCGGAGACT GTGCCAGACT TTTAAAAAGA GCATTTCCAC
  841 GACAGAGATA CTCAGATCCT CCCAAAGATC GTCTTTGGTA AATGGGAAGC GGCCACTCAA
901 TAGATGGGAG AAGC
//
Primer: M13_-40
Sequence file: RDB15792_A8Bec.seq check
>04225_15792_A8Be_M13_-40_D02_11_ABI24.ab1
    1 ACCAAGTTAA CCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC
   61 AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT
  121 GAGCTAACTT ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC
  181 GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG
  241 CCAGGGTGGT TTTTCTTTTC ACCAGTGAGA CGGGCAACAG CTGATTGCCC TTCACCGCCT
  301 GGCCCTGAGA GAGTTGCAGC AAGCGGTCCA CGCTGGTTTG CCCCAGCAGG CGAAAATCCT
  361 GTTTGATGGT GGTTAACGGC GGGATATAAC ATGAGCTGTC TTCGGTATCG TCGTATCCCA
  421 CTACCGAGAT ATCCGCACCA ACGCGCAGCC CGGACTCGGT AATGGCGCGC ATTGCGCCCA
  481 GCGCCATCTG ATCGTTGGCA ACCAGCATCG CAGTGGGAAC GATGCCCTCA TTCAGCATTT
  541 GCATGGTTTG TTGAAAACCG GACATGGCAC TCCAGTCGCC TTCCCGTTCC GCTATCGGCT
  601 GAATTTGATT GCGAGTGAGA TATTTATGCC AGCCAGCCAG ACGCAGACGC GCCGAGACAG
  661 AACTTAATGG GCCCGCTAAC AGCGCGATTT GCTGGTGACC CAATGCGACC AGATGCTCCA
  721 CGCCCAGTCG CGTACCGTCT TCATGGGAGA AAATAATACT GTTGATGGGT GTCTGGTCAG
  781 AGACATCAAG AAATAACGCC GGAACATTAG TGCAGGCAGC TTCCACAGCA ATGGCATCCT
  841 GGTCATCCAG CGGATAGTTA ATGATCAGCC CACTGACGCG TTGCGCGAGA AGATTGTGCA
  901 CCGCCGCTTT ACAGGCTTCG ACGCCGCTTC GTTCTACCAT CGACACCACC ACGCTGGCAC
  961 CCAGTTGATC GGCGCGAGAT TTAATCGCCG CGACAATTTG CGACGGCGCG TGCAGGGCCA
 1021 GACTGGAGTG GCACGCCAAT CAGCCACGAC TGTTTGCCCG CCAGTTGTGT GCACGCCGCT
 1081 GGGATGTATT CAGCCTCCGG CATCGGCGGC TCAACTTTTT CCCGCGTTTC GGCAGAAACG
1141 GTGTGCCTGT GGATCCTTG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

protocol Nishi, N., A note on expression and purification of recombinant galectins. Glycoforum Vol.23 (5), A15 (2020). [link to RRC of NBRP]