Please review the QC test results indicated by icon below as well as clone information before placing your order.

CS-RfA-EF-mRFP1

Backbone vector plasmid of shRNA expressing lentivirus construct by Gateway(R) cloning with mRFP1 marker driven by EF-1 alpha promoter.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Ordering	Order form [Credit Card Payment]  [Bank Transfer Payment] MTA, for use for not-for-profit academic purpose)[Word] Please visit Ordering instruction.[link]
Remarks	Remember that you will be working with samples containing infectious virus. Use Survival2 (Invitrogen) or equivalent to amplify this clone. This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.

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提供条件	本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利機関による非営利学術研究用)[Word] 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。 手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 このクローンの増殖にはSurvival2 (Invitrogen)あるいは同等の宿主を使用してください。 Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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Materials & Methods section:

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB20218_B3Kep1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F (Pr0168) Region: EF-1alpha pro,mRFP1 5' Sequence file: RDB20218_B3Kea.seq
>05736_20218_B3Ke_3_EF1a-C_F_A01_01_ABI24.ab1     1 NNNNNNNNNN NNNNTTGNNT TTGGNNCTTG GTTNNTTCTC NAGCCTCAGA CAGTGGTTCA    61 AAGTTTTTTT CTTCCATTTC AGGTGTCGTG AATTAGATCC GGCGAGGGCA GAGGAAGTCT   121 TCTAACATGC GGTGACGTGG AGGAAAATCC CGGGCCTATG GCCTCCTCCG AGGACGTCAT   181 CAAGGAGTTC ATGCGCTTCA AGGTGCGCAT GGAGGGCTCC GTGAACGGCC ACGAGTTCGA   241 GATCGAGGGC GAGGGCGAGG GCCGCCCCTA CGAGGGCACC CAGACCGCCA AGCTGAAGGT   301 GACCAAGGGC GGCCCCCTGC CCTTCGCCTG GGACATCCTG TCCCCTCAGT TCCAGTACGG   361 CTCCAAGGCC TACGTGAAGC ACCCCGCCGA CATCCCCGAC TACTTGAAGC TGTCCTTCCC   421 CGAGGGCTTC AAGTGGGAGC GCGTGATGAA CTTCGAGGAC GGCGGCGTGG TGACCGTGAC   481 CCAGGACTCC TCCCTGCAGG ACGGCGAGTT CATCTACAAG GTGAAGCTGC GCGGCACCAA   541 CTTCCCCTCC GACGGCCCCG TAATGCAGAA GAAGACCATG GGCTGGGAGG CCTCCACCGA   601 GCGGATGTAC CCCGAGGACG GCGCCCTGAA GGGCGAGATC AAGATGAGGC TGAAGCTGAA   661 GGACGGCGGC CACTACGACG CCGANGTCAA GACCACCTAC ATGGCCAAGA AGCCCGTGCA   721 GCTGCCCGGC GCCTACNAGA NCGACATCAA GCTGGACATC ACCTCCCACA ACGNGGACTA   781 CACCATCGNG GAACAGTACG ANCGCGCCGA GGGCCGCCAC TCCACCGGCG CCTAANCGGN   841 CGCACTCNAN GGNNCNAGCT TATCGATAAT CAANCNCTGG ANNANANNNT TGTGAAAGAN 901 NGANNNGNNN TTCTTNNCNA TGNTGCTCCN TTNNNNCNNN NNNNNNN //
Primer: env_F (Pr0258) Region: cPPT_CTS,attR1,CmR Sequence file: RDB20218_B3Keb.seq
>05736_20218_B3Ke_3_env_F_A01_01_ABI08.ab1     1 NNNNNNNNNN NNNNNCNNNN ANNNNNNGNN NCTACAAATG GCAGTATTCA TCCACAATTT    61 TAAAAGAAAA NGNNNNNNNG GGGGGTACAG TGCAGGGGAA AGAATAGTAN NNNNNNNNNC   121 AACAGACATA CAAACTAAAG AATTACAAAA ACAAATTACA AAAATTCAAA ATTTTCGGGT   181 TTATTACAGG GACAGCAGAA ATTCACTTTG AAATCACAAG TTTGTACAAA AAAGCTGAAC   241 GAGAAACGTA AAATGATATA AATATCAATA TATTAAATTA GATTTTGCAT AAAAAACAGA   301 CTACATAATA CTGTAAAACA CAACATATCC AGTCACTATG GCGGCCGCAT TAGGCACCCC   361 AGGCTTTACA CTTTATGCTT CCGGCTCGTA TAATGTGTGG ATTTTGAGTT AGGATCCGTC   421 GAGATTTTCA GGAGCTAAGG AAGCTAAAAT GGAGAAAAAA ATCACTGGAT ATACCACCGT   481 TGATATATCC CAATGGCATC GTAAAGAACA TTTTGAGGCA TTTCAGTCAG TTGCTCAATG   541 TACCTATAAC CAGACCGTTC AGCTGGANAT TACGGCCTTT TTAAAGACCG TAAAGAAAAA   601 TAAGCACAAG TTTTATCCGG CCTTTATTCA CATTCTTGCC CGCCTGANGA ANGCTCNTCC   661 GGAATTCCGT ANGGCAATGA AAGACGGTGA NCTGGTGANN TGGGNANAGT GNTNACCCNN 721 NGNTNACNCN NNTTTN //
Primer: PRE_rev (Pr0250) Region: PRE,mRFP1 3' Sequence file: RDB20218_B3Kec.seq
>05736_20218_B3Ke_3_PRE_rev_B01_04_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNCANNATA GTTAAGANNA CCAGTCAATC TTTCACAAAT    61 TTTGTAANCC AGAGGTTGAT TATCGATAAG CTTGATCCCT CGAGTGCGGC CGCTTAGGCG   121 CCGGTGGAGT GGCGGCCCTC GGCGCGCTCG TACTGTTCCA CGATGGTGTA GTCCTCGTTG   181 TGGGAGGTGA TGTCCAGCTT GATGTCGGTC TTGTAGGCGC CGGGCAGCTG CACGGGCTTC   241 TTGGCCATGT AGGTGGTCTT GACCTCGGCG TCGTAGTGGC CGCCGTCCTT CAGCTTCAGC   301 CTCATCTTGA TCTCGCCCTT CAGGGCGCCG TCCTCGGGGT ACATCCGCTC GGTGGAGGCC   361 TCCCAGCCCA TGGTCTTCTT CTGCATTACG GGGCCGTCGG AGGGGAAGTT GGTGCCGCGC   421 AGCTTCACCT TGTAGATGAA CTCGCCGTCC TGCAGGGAGG AGTCCTGGGT CACGGTCACC   481 ACGCCGCCGT CCTCGAAGTT CATCACGCGC TCCCACTTGA AGCCCTCGGG GAAGGACAGC   541 TTCAAGTAGT CGGGGATGTC GGCGGGGTGC TTCACGTAGG CCTTGGAGCC GTACTGGAAC   601 TGAGGGGACA GGATGTCCCA GGCGAAGGGC AGGGGGCCGC CCTTGGTCAC CTTCAGCTTG   661 NCGGTCTGGG TGCCCTCGTA NGGGCGGCCC TCGCCCTCGC CCTCNATCTC GAACTCGTGG   721 CCGTTCACGG AGCCCTCCAT GCGCACCTTG AAGCGCATGA ACTCCTTGAT GACGTCCTCG   781 GNNGNNGCCA TANGNCCGGN NNTTTCCTCC ACGTCACNGC ATGTTANAAG ANTTCCTCTG   841 CCNTCNCCGG NNCTNATTCA CGACANCNNN AATNNAAGAA AAAAANNTTT NNNNCACTNT   901 NNGANGNTNN NNNNTGNANC CNNGNTNCNA ANNNNAAANN NGNNNNNNNN CNNNNNNNAN 961 TTNNNTNNNN NNNNNN //
Primer: EF1a-N_R (Pr0024) Region: attR2,ccdB Sequence file: RDB20218_B3Ked.seq
>05736_20218_B3Ke_3_EF1a-N_R_D01_10_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNG GCNNNGTGCG CTCTGCCCNC TNNCGGGCAC CGGNNCCTCA    61 CGAAGCTTGN ANNATCACCA CTTTGTACAA GAAAGCTGAA CGAGAAACGT AAAATGATAT   121 AAATATCAAT ATATTAAATT AGATTTTGCA TAAAAAACAG ACTACATAAT ACTGTAAAAC   181 ACAACATATC CAGTCACTAT GGTCGACCTG CAGACTGGCT GTGTATAAGG GAGCCTGACA   241 TTTATATTCC CCAGAACATC AGGTTAATGG CGTTTTTGAT GTCATTTTCG CGGTGGCTGA   301 GATCAGCCAC TTCTTCCCCG ATAACGGAGA CCGGCACACT GGCCATATCG GTGGTCATCA   361 TGCGCCAGCT TTCATCCCCG ATATGCACCA CCGGGTAAAG TTCACGGGAG ACTTTATCTG   421 ACAGCAGACG TGCACTGGCC AGGGGGATCA CCATCCGTCG CCCGGGCGTG TCAATAATAT   481 CACTCTGTAC ATCCACAAAC AGACGATAAC GGCTCTCTCT TTTATAGGTG TAAACCTTAA   541 ACTGCATTTC ACCAGCCCCT GTTCTCGTCA GCAAAAGAGC CGTTCATTTC AATAAACCGG   601 GCGACCTCAG CCATCCCTTC CTGATTTTCC GCTTTCCAGC GTTCGGCACG CAGACGACGG   661 GCTTCATTCT GCATGGTTGT GCTTACCAGA CCGGANATAT TGACATCATA TATGCCTTGA   721 GCAACTGATA GCTGTCGCTG TCAACTGTCA CTGTAATACG CTGCTTCATA GCATACCTCT   781 TTTTTGACAT ACTTCGGGTA TACATATCAG TATATATTCT TATACCGCAA AAATCAGCGC   841 GCAAATACGC ATACNGTTAT CTGGCTTTNA NNAAGCCNGA TCCNCNCNTT TACNCCCNGN 901 CNNGNNNNTC ANCGNANNAN NNNNGNNNNT CANNNNNNNN NN //
Primer: CMV_Forward (Pr0016) Region: CMV pro,5' LTR,HIV-1 phi Sequence file: RDB20218_B3Kee.seq
>05736_20218_B3Ke_3_CMV_Forward_E01_13_ABI24.ab1     1 NNNNNNNNNN NNNNTTGNNG CNNNGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG    61 CAGCGCGTTT TGCCTGTACT GGGTCTCTCT GGTTAGACCA GATCTGAGCC TGGGAGCTCT   121 CTGGCTAACT AGGGAACCCA CTGCTTAAGC CTCAATAAAG CTTGCCTTGA GTGCTTCAAG   181 TAGTGTGTGC CCGTCTGTTG TGTGACTCTG GTAACTAGAG ATCCCTCAGA CCCTTTTAGT   241 CAGTGTGGAA AATCTCTAGC AGTGGCGCCC GAACAGGGAC TTGAAAGCGA AAGGGAAACC   301 AGAGGAGCTC TCTCGACGCA GGACTCGGCT TGCTGAAGCG CGCACGGCAA GAGGCGAGGG   361 GCGGCGACTG GTGAGTACGC CAAAAATTTT GACTAGCGGA GGCTAGAAGG AGAGAGATGG   421 GTGCGAGAGC GTCAGTATTA AGCGGGGGAG AATTAGATCG CGATGGGAAA AAATTCGGTT   481 AAGGCCAGGG GGAAAGAAAA AATATAAATT AAAACATATA GTATGGGCAA GCAGGGAGCT   541 AGAACGATTC GCAGTTAATC CTGGCCTGTT AGAAACATCA GAAGGCTGTA GACAAATACT   601 GGGACAGCTA CAACCATCCC TTCAGACAGG ATCAGAAGAA CTTAGATCAT TATATAATAC   661 AGTAGCAACC CTCTATTGTG TGCATCAAAG GATAGAGATA AAAGACACCA AGGAAGCTTT   721 AGACAAGATA GAGGAAGAGC AAAACAAAAG TAAGACCACC GCACAGCAAG CGGCCGCNGA   781 TCTTCAGACC TGGNNGNNGA NNNATGANGG NNAATTGGAG AANTGAATTA TATAAATATN 841 ANGTANTAAA AANTTGANCC NTTANGNNTA NCNCCCNCCA ANGNNAANNN NNNNNNN //
Primer: bGH_rev3 (Pr0885) Region: bGH pA,3' LTR Sequence file: RDB20218_B3Kef.seq
>05736_20218_B3Ke_3_bGH_rev3_G01_19_ABI24.ab1     1 NNNNNNNNNN NNNNGGNNGN NGGGNAACAA CAGNTGGCTG GCAACTAGAA GGCACAGTCG    61 AGGCTGATCA GCGGGTTTAA ACGGGCCCTG CTAGAGANTT TCCACACTGA CTAAAAGGGT   121 CTGAGGGATC TCTAGTTACC AGAGTCACAC AACAGACGGG CACACACTAC TTGAAGCACT   181 CAAGGCAAGC TTTATTGAGG CTTAAGCAGT GGGTTCCCTA GTTAGCCAGA GAGCTCCCAG   241 GCTCAGATCT GGTCTAACCA GAGAGACCCA GTACAGTCCG GATGCAGCTC TCGGGCCATG   301 TGATGAAATG CTAGGCGGCT GTCAAACCTC CACTCTAATA CTTCTCTCTC CGGGTCATCC   361 ATCCCATGCA GGCTCACAGG GTGTAACAAG CGGGTGTTCT CTCCTTCATT GGCTTCTTCT   421 ACCTTCTCTT GCTCAACTGG TACTAGCTTG TAGCACCATC CAAAGGTCAG TGGATATCTG   481 ATCCCTGGCC CTGGTGTGTA GTTCTGCCAA TCAGGGAAGT AGCCTTGTGT GTGGTAGATC   541 CACAGATCAA GGATATCTTG TCTTCGTTGG GAGTGAATTA GCCCTTCCAG TCCCCCCTTT   601 TCTTTTAAAA AGTGGCTAAG ATCTACAGCT GCCTTGTAAG TCATTGGTCT TAAAGGTACC   661 TGANGTGTGA CTGGAAAACC CACCTCCTCC TCCTCTTGTG CTTCTAGCCA GGCACAATCA   721 GCATTGGTAG CTGCTGTATT GCTACTTGTG ATTGCTCCAT GTTTTTCTAG GTCTCGACGG   781 TATCGATGCG GGGNNGCGGC CCNAANGNNN NTCCGANTCG TCTGANGGCG AANGNGAANA 841 NGCGGAANAN GCCGCANNNN NNNNNN //
Primer: RRE-M_R (Pr0651) Region: RRE,HIV-1 phi Sequence file: RDB20218_B3Keg.seq
>05736_20218_B3Ke_3_RRE-M_R_F01_16_ABI24.ab1     1 NNNNNNNNNN NNANNNNNGC TGTTGCGCCT CANTAGCCCT CAGCAAATTG TTCTGCTGCT    61 GCACTATACC AGACAATAAT TGTCTGGCCT GTACCGTCAG CGTCATTGAC GCTGCGCCCA   121 TAGTGCTTCC TGCTGCTCCC AAGAACCCAA GGAACAAAGC TCCTATTCCC ACTGCTCTTT   181 TTTCTCTCTG CACCACTCTT CTCTTTGCCT TGGTGGGTGC TACTCCTAAT GGTTCAATTT   241 TTACTACTTT ATATTTATAT AATTCACTTC TCCAATTGTC CCTCATATCT CCTCCTCCAG   301 GTCTGAAGAT CAGCGGCCGC TTGCTGTGCG GTGGTCTTAC TTTTGTTTTG CTCTTCCTCT   361 ATCTTGTCTA AAGCTTCCTT GGTGTCTTTT ATCTCTATCC TTTGATGCAC ACAATAGAGG   421 GTTGCTACTG TATTATATAA TGATCTAAGT TCTTCTGATC CTGTCTGAAG GGATGGTTGT   481 AGCTGTCCCA GTATTTGTCT ACAGCCTTCT GATGTTTCTA ACAGGCCAGG ATTAACTGCG   541 AATCGTTCTA GCTCCCTGCT TGCCCATACT ATATGTTTTA ATTTATATTT TTTCTTTCCC   601 CCTGGCCTTA ACCGAATTTT TTCCCATCGC GATCTAATTC TCCCCCGCTT AATACTGACG   661 CTCTCGCACC CATCTCTCTC CTTCTAGCCT CCGCTAGTCA AAATTTTTGG CGTACTCACC   721 AGTCGCCGCC CCTCGCCTCT TGCCGTGCGC GCTTCANCAA GCCGANTCCT GCGTCGAGAG   781 AGCTCCTCTG GTTTCCCTTT CGCTTTCNAG TCCCNGTTCG GGCGCCACTG CTANAGANTT   841 TCCACACNNN NNNAAAGGGT CTGANGNNTC TCTANTTACC AGANNNNCAC AACANANGGG   901 NNCNCACTAN TNNNANCACT NNANNNNNNN NNTTNNNNNN NNNTNNNNNN NNNGNNTNNC 961 NNNNNNNNNN //
Primer: SV40pro_F (Pr0110) Region: Zeo Sequence file: RDB20218_B3Keh.seq
>05736_20218_B3Ke_3_SV40pro_F_H01_22_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNTGNNG NNGCTTTTTT GGNNGCCTAG GCTTTTGCAA    61 AAAGCTCCCG GGANCTTGTA TATCCATTTT CGGATCTGAT CAGCACGTGT TGACAATTAA   121 TCATCGGCAT AGTATATCGG CATAGTATAA TACGACAAGG TGAGGAACTA AACCATGGCC   181 AAGTTGACCA GTGCCGTTCC GGTGCTCACC GCGCGCGACG TCGCCGGAGC GGTCGAGTTC   241 TGGACCGACC GGCTCGGGTT CTCCCGGGAC TTCGTGGAGG ACGACTTCGC CGGTGTGGTC   301 CGGGACGACG TGACCCTGTT CATCAGCGCG GTCCAGGACC AGGTGGTGCC GGACAACACC   361 CTGGCCTGGG TGTGGGTGCG CGGCCTGGAC GAGCTGTACG CCGAGTGGTC GGAGGTCGTG   421 TCCACGAACT TCCGGGACGC CTCCGGGCCG GCCATGACCG AGATCGGCGA GCAGCCGTGG   481 GGGCGGGAGT TCGCCCTGCG CGACCCGGCC GGCAACTGCG TGCACTTCGT GGCCGAGGAG   541 CAGGACTGAC ACGTGCTACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC   601 TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGN TCTCATGCTG   661 GAGTTCTTCG CCCACCCCAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT   721 AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC   781 NAACTCATCA ATGTATCTTA TCATGTCTGT ATACCGTCNA NCTCTANCTA GANCTNGGCG   841 TAATCNTGGT CATANCTGTT TCCNGTGNGA AATTGTTATC CNNNCACNAT TCCNCNCNAC   901 NTACNANCNN GNNNNNTAAA NNGNNANNNC NNNGGNNNNC NNNNNGNNNN NNCNNNNNNN 961 NNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2024.03.26

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