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CS-CA-MCS (#RDB05963)

Backbone vector plasmid of lentivirus construct with CAG promoter.

Clone info. Backbone vector plasmid of lentivirus construct with CAG promoter.
Comment Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394).
Vector backbone Plasmid
Selectable markers Ampicillin (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Growth conditions 37C, LB+Amp
Depositor|Developer Miyoshi, Hiroyuki |
 
Sequence (full) RDB05963hts01.seq checkAssembled from experimentally sequenced data.
Publication Unpublished bioresource
Test sheet RDB15876_A8D9p1-2.pdf 
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Ordering instruction.[link] 
Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Remarks Remember that you will be working with samples containing infectious virus.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB05963 CS-CA-MCS DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Ordering instruction of plasmids [in Japanese] [in English]

How to cite this biological resource

Materials & Methods section:

The CS-CA-MCS was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05963).

Reference section:

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

user_report Hori, T., Trophoblast stem cell-based organoid models of the human placental barrier. Nat. Commun. 15 (1): 962 (2024). PMID 38332125. [link to RRC of NBRP]
user_report Shibata, S., Modeling embryo-endometrial interface recapitulating human embryo implantation. Sci. Adv. 10 (8): eadi4819 (2024). PMID 38394208. [link to RRC of NBRP]
user_report Kobayashi, N., The microRNA cluster C19MC confers differentiation potential into trophoblast lineages upon human pluripotent stem cells. Nat. Commun. 13 (1): 3071 (2022). PMID 35654791. [link to RRC of NBRP]
user_report Ikeda, A., Colorectal Cancer-Derived CAT1-Positive Extracellular Vesicles Alter Nitric Oxide Metabolism in Endothelial Cells and Promote Angiogenesis. Mol. Cancer Res. 19 (5): 834-846 (2021). PMID 33579815. [link to RRC of NBRP]
user_report Matsumura, H., Distinct types of stem cell divisions determine organ regeneration and aging in hair follicles. Nat. Aging 1 (2): 190-204 (2021). PMID 37118636. [link to RRC of NBRP]
user_report Sampilvanjil, A., Cigarette smoke extract induces ferroptosis in vascular smooth muscle cells. Am. J. Physiol. Heart Circ. Physiol. 318 (3): H508-H518 (2020). PMID 31975626. [link to RRC of NBRP]
user_report Ouwendijk, W.J.D., Varicella-zoster virus VLT-ORF63 fusion transcript induces broad viral gene expression during reactivation from neuronal latency. Nat. Commun. 11 (1): 6324 (2020). PMID 33303747. [link to RRC of NBRP]
user_report Frazier, A.E., Fatal Perinatal Mitochondrial Cardiac Failure Caused by Recurrent De Novo Duplications in the ATAD3 Locus. Med (N Y) 2 (1): 49-73 (2020) PMID 33575671. [link to RRC of NBRP]
user_report Borna, N.N., Mitochondrial ribosomal protein PTCD3 mutations cause oxidative phosphorylation defects with Leigh syndrome. Neurogenetics 20 (1): 9-25 (2019). PMID 30607703. [link to RRC of NBRP]
user_report Mitani, A., Characterization of doxycycline-dependent inducible Simian Virus 40 large T antigen immortalized human conjunctival epithelial cell line. PLoS One 14 (9): e0222454 (2019). PMID 31509592. [link to RRC of NBRP]
user_report Takahashi, S., Loss of p57 KIP2 expression confers resistance to contact inhibition in human androgenetic trophoblast stem cells. Proc. Natl. Acad. Sci. U.S.A. pii: 201916019 (2019). PMID 31792181. [link to RRC of NBRP]
user_report Karasawa, T., Saturated Fatty Acids Undergo Intracellular Crystallization and Activate the NLRP3 Inflammasome in Macrophages Arterioscler. Thromb. Vasc. Biol. 38 (4): 744-756 (2018). PMID 29437575. [link to RRC of NBRP]
user_report Kawashima, A., ARIH2 Ubiquitinates NLRP3 and Negatively Regulates NLRP3 Inflammasome Activation in Macrophages. J. Immunol. 199 (10): 3614-3622 (2017). PMID 29021376. [link to RRC of NBRP]
user_report Kohda, M., A Comprehensive Genomic Analysis Reveals the Genetic Landscape of Mitochondrial Respiratory Chain Complex Deficiencies. PLoS Genet. 12 (1): e1005679 (2016). PMID 26741492. [link to RRC of NBRP]
user_report Kishita, Y., Intra-mitochondrial Methylation Deficiency Due to Mutations in SLC25A26. Am. J. Hum. Genet. 97 (5): 761-768 (2015). PMID 26522469. [link to RRC of NBRP]
user_report Okada, K., Enhanced flexibility of place discrimination learning by targeting striatal cholinergic interneurons. Nat. Commun., 5: 3778 (2014). PMID 24797209. [link to RRC of NBRP]
user_report Nanba, D., Actin filament dynamics impacts keratinocyte stem cell maintenance. EMBO Mol Med., 5 (4): 640-653 (2013). PMID 23554171. [link to RRC of NBRP]
user_report Matsushita, N., Doxycycline-dependent inducible and reversible RNA interference mediated by a single lentivirus vector. Biosci. Biotechnol. Biochem., 77 (4): 776-781 (2013). PMID 23563548. [link to RRC of NBRP]
user_report Tang, H., CD134 is a cellular receptor specific for human herpesvirus-6B entry. Proc. Natl. Acad. Sci. U.S.A., 110 (22): 9096-9099 (2013). PMID 23674671. [link to RRC of NBRP]
user_report Yaguchi, M., Characterization of the properties of seven promoters in the motor cortex of rats and monkeys after lentiviral vector-mediated gene transfer. Hum. Gene Ther. Methods, 24 (6): 333-344 (2013). PMID 23964981. [link to RRC of NBRP]
user_report Tamura, K., A glass-coated tungsten microelectrode enclosing optical fibers for optogenetic exploration in primate deep brain structures. J. Neurosci. Methods, 211 (1): 49-57 (2012). PMID 22971353. [link to RRC of NBRP]
user_report Fujita, K., Effects of a brain-engraftable microglial cell line expressing anti-prion scFv antibodies on survival times of mice infected with scrapie prions. Cell. Mol. Neurobiol. 31 (7): 999-1008 (2011). PMID 21516351. [link to RRC of NBRP]
user_report Tsubota, T., Optogenetic manipulation of cerebellar purkinje cell activity in vivo. PLoS One 6 (8): e22400 (2011). PMID 21850224. [link to RRC of NBRP]
user_report Nishie T., Beta4-galactosyltransferase-5 is a lactosylceramide synthase essential for mouse extra-embryonic development. Glycobiology., 20, 1311-1322 (2010). PMID 20574042. [link to RRC of NBRP]
user_report Ohashi Y., A bicistronic lentiviral vector-based method for differential transsynaptic tracing of neural circuits. Mol. Cell. Neurosci. 46 (1): 136-147. PMID 20816792. [link to RRC of NBRP]
user_report Inuzuka T., Transcription factor 8 activates R-Ras to regulate angiogenesis. Biochem. Biophys. Res. Commun., 379, 510-513 (2009). PMID 19116136. [link to RRC of NBRP]