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pAdex MBP-SwaI-SV40pA (#RDB06014)

Shuttle vector to generate rAd with MBP promoter

Clone info. Shuttle vector to generate rAd. Inserted sequence is regulated under the control of MBP promoter.
Comment MBP promoter-SwaI-SV40pA (2.2kbp) fragment was cloned on SwaI site of pAdex1cw. Produce adenovirus by COS-TPC method.
Vector backbone pAxcw (Cosmid, use packaging extracts for transforming E. coli host)
Selectable markers Amp^r
Gene/insert name Mouse MBP
Depositor|Developer Hashimoto, Mitsuhiro |
 

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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06014 pAdex MBP-SwaI-SV40pA DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pAdex MBP-SwaI-SV40pA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06014).

Reference section:

Hashimoto, M., Aruga, J., Hosoya, Y., Kanegae, Y., Saito, I., Mikoshiba, K., Hum. Gene Ther., 7 (2): 149-158 (1996). PMID 8788166. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13140_A5Br.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pAdex_F
Sequence file: RDB13140_A5Bra.seq check
>06014_13140_A5Br_pAdex_F_C10_07.ab1
    1 CAGTTACGCC ACACTCGCAG GGTCTGCACC ATGGTGCGGG TCTCATCGTA CCTCAGCACC
   61 TTCCAGATCC ATCGTCGCGA GTCGACAATC GATTTGGCCG CTCTAGAACT AGTGGATCTT
  121 ATTCCTCACC GTGGCTTTAA CACTTAGAGA AAATGCATCC CCTCTAATCA ATAAGTCATC
  181 GACAGTGGGT AGATGGAGGA ACGGCAGTGC GTAGTAGGAT GCGTGCAAGC ATAGTCTCGT
  241 GCATGGGTGC ATAGATCGCT GGGCAGGTGG ACAAGGTGGG GGTGGATAAA GAAGTGGGTA
  301 GATGATTGAT GTTAGGTAAA TATCACTGGG TGGACAGATG GGTGGTAGGT GGATGGATGG
  361 TTAGAATAGT CAGAAGAGGG ATGGATTGAT AAGGTGAACA GATGATAAAT GGGTGATAGA
  421 CTGGAAGGGT TGTCAAAAGA GGATAAGGGA AGTGTGAGCT AGCCGTATTT CTAAGGTCAG
  481 TAATAGAGTT GGGAGAAGAG GTTAAGTTAC ATCCATTTAA ACCTCACACG AAGCTGAGAG
  541 GGAATGGACT TGCTGCCGTT GGTGAGGAAA GCGTTGCATT TCCCGTGTGC TTGGTTGTGA
  601 AGTGCTCAGG TCCCACATGA AGCAGTCAGG TTACTGCGGC TTACAGAGGA GCCAGATCCA
  661 AATGCCCCGA GTAAGCACGT CCCCGAGCCA GAGGCCTCCA GCGGAATCCG GGAGAGGGAT
  721 TGCTCAGTGC CCTGCTTCCC TGGACTGTAA GCTGCAGAAA GATGTGGGAA GTCCTGTTCT
  781 CCACTGAGAA CACTAAAAGC ACCTTTTGTC AAACGACCGC TTCACATCTG GGGCTTGTGC
  841 ACTGGTGGCC TTTTAAACCA GAGACAACCC ACAAGATACC TAACCTGCGG GGCTCTCTGG
  901 TACAGTGAGC AACTCAGGAA ATGCTTTGGC TTGATTGCTG TGGGCTCTCA GCCATCGCCC
  961 TCTGGAGTGA TTCTTTTAAT GAGAACCTGA AGATTGTCCC CTGAGCCATG TATACAAGCA
 1021 AGCTCAATCC AGGTTAGCTC CCTCTGGTTG GGGGCAAGCT AACGATGCTT CCTGGACCCC
 1081 GCGCGTAACC TGGTGGCGTT TTATAAGAGA CAAGCTAGTT TCAAGTATCT AGTGTAGAAT
1141 GACGGACCTT TGTTCTCCCC CTTC
//
Primer: pJB8-Forward
Sequence file: RDB13140_A5Brb.seq check
>06014_13140_A5Br_pJB8-Forward_D10_10.ab1
    1 GACTGGCATA TGATATGAGG GGGTGGAGTT TGTGACGTGG CGCGGGGCGT GGGAACGGGG
   61 CGGGTGACGT AGTAGTGTGG CGGAAGTGTG ATGTTGCAAG TGTGGCGGAA CACATGTAAG
  121 CGACGGATGT GGCAAAAGTG ACGTTTTTGG TGTGCGCCGG TGTACACAGG AAGTGACAAT
  181 TTTCGCGCGG TTTTAGGCGG ATGTTGTAGT AAATTTGGGC GTAACCGAGT AAGATTTGGC
  241 CATTTTCGCG GGAAAACTGA ATAAGAGGAA GTGAAATCTG AATAATTTTG TGTTACTCAT
  301 AGCGCGTAAT ATTTGTCTAG GGCCGCGGGG ACTTTGACCG TTTACGTGGA GACTCGCCCA
  361 GGTGTTTTTC TCAGGTGTTT TCCGCGTTCC GGGTCAAAGT TGGCGTTTTA TTATTATAGT
  421 CAGCATCGAT TTGGCCGCTC GGGCCCCCCC TCGAGGTCGA CGGTATCGAT AAGCTGATCC
  481 AGACATGATA AGATACATTG ATGAGTTTGG ACAAACCACA ACTAGAATGC AGTGAAAAAA
  541 ATGCTTTATT TGTGAAATTT GTGATGCTAT TGCTTTATTT GTAACCATTA TAAGCTGCAA
  601 TAAACAAGTT AACAACAACA ATTGCATTCA TTTTATGTTT CAGGTTCAGG GGGAGGTGTG
  661 GGAGGTTTTT TAAAGCAAGT AAAACCTCTA CAAATGTGGT ATGGCTGATT ATGATCTCTA
  721 GTCAAGGCAC TATACATCAA ATATTCCTTA TTAACCCCTT TACAAATTAA AAAGCTAAAG
  781 GTACACAATT TTTGAGCATA GTTATTAATA GCAGACACTC TATGCCTGTG TGGAGTAAGA
  841 AAAAACAGTA TGTTATGATT ATAACTGTTA TGCCTACTTA TAAAGGTTAC AGAATATTTT
  901 TCCATAATTT TCTTGTATAG CAGTGCAGCT TTTTCCTTTG TGGTGTAAAT AGCAAAGCAA
  961 GCAAGAGTTC TATTACTAAA CACAGCATGA CTCAAAAAAC TTAGCAAATT CTGAAGGAAA
 1021 GTCCCTTGGG GGTCTTCTAC CCTTTCTCTT CTTTTTTTTG GAGGGAGTAG AATGGTTGGA
 1081 GAGTCAGCCA GTTAGCCCTC ATCATTCCAC TTAGATGGCC ATTTCCTTCT GGAGCCAAAA
 1141 AACAAGGGTG TTTTCTCTTC CCACTTTAAA GAGGGCGACG ATTTTCTCTA CTCACACACC
1201 CC
//
Primer: SV40polyA-R
Sequence file: RDB13140_A5Brc.seq check
>06014_13140_A5Br_SV40poly_R_C12_09.ab1
    1 AAAATGCTGA GTATGGCTGA TTATGATCTC TAGTCAAGGC ACTATACATC AAATATTCCT
   61 TATTAACCCC TTTACAAATT AAAAAGCTAA AGGTACACAA TTTTTGAGCA TAGTTATTAA
  121 TAGCAGACAC TCTATGCCTG TGTGGAGTAA GAAAAAACAG TATGTTATGA TTATAACTGT
  181 TATGCCTACT TATAAAGGTT ACAGAATATT TTTCCATAAT TTTCTTGTAT AGCAGTGCAG
  241 CTTTTTCCTT TGTGGTGTAA ATAGCAAAGC AAGCAAGAGT TCTATTACTA AACACAGCAT
  301 GACTCAAAAA ACTTAGCAAT TCTGAAGGAA AGTCCTTGGG GTCTTCTACC TTTCTCTTCT
  361 TTTTTGGAGG AGTAGAATGT TGAGAGTCAG CAGTAGCCTC ATCATCACTA GATGGCATTT
  421 CTTCTGAGCA AAACAGGTTT TCCTCATTAA AGGCATTCCA CCACTGCTCC CATTCATCAG
  481 TTCCATAGGT TGGAATCTAA AATACACAAA CAATTAGAAT CAGTAGTTTA ACACATTATA
  541 CACTTAAAAA TTTTATATTT ACCTTAGAGC TTTAAATCTC TGTAGGTAGT TTGTCCAATT
  601 ATGTCACACC ACAGAAGTAA GGTTCCTTCA CAAAGATCCG ATAAGCTTGA TATCGAATTC
  661 CTGCAGCCCG ATTTAAATGG GGGATCGATC CACTAGTCCG GAAGCTGCTG TGGGGTCTTC
  721 TTGGATGGTC TGAAGCTCGT CGGACTCTGA GGGCCTGTCT TTGAAGGTGT TGTCCTCCCT
  781 TCCCGGGGCA TCTCGGGAAA AGAGGCGGAT CAAGTGGGGG CGGTTCCCTG GGTCAGCTGG
  841 GTGGGCGCCT TGCCAGTTAT TCTTTGGGTC TGCTGTGTGC TTGGAGTCTG TCACCGCCGT
  901 GTCCTCAGCC GAGGTCCCAA TTGTTCTGGA TCGCATCTGG CCTCCTCTGC AACACAATGG
  961 AGATATGAAT GTGGGGCTGG TACGAGGACG TACAAGAGGA CAAGCACGCA TTTTCTTTCC
 1021 TGAGGGGTAG TTGAAGTATA ACATAGGTCT TGACTATATG AAACGACTCG ACCAAGTATA
1081 TGCTCGACTG CGGAGGGCAT GCAG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Hashimoto, M., Hum. Gene Ther., 7 (2): 149-158 (1996). PMID 8788166. [link to RRC of NBRP]