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pAdex CAG-SwaI-IRES-EGFP

Shuttle vector to generate rAd with CAG expression unit.

Catalog number RDB06016
Resource name pAdex CAG-SwaI-IRES-EGFP
Clone info. Shuttle vector to generate rAd. Inserted sequence is regulated under the control of CAG expression unit.
Comment SwaI-IRES-EGFP fragment (1.3kbp) was cloned on SwaI site of pAdex1CAw. IRES: internal ribosomal entry site, EGFP: enhanced green fluorecense protein. Produce adenovirus by COS-TPC method.
Vector backbone pAxCAwt (Cosmid, use packaging extracts for transformation)
Selectable markers Amp^r
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Hashimoto, Mitsuhiro |
Other clones in our bank

External Database
Aequorea victoria GFP

            Reference sequence
              
             

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution This material is provided with written consent of Depositor. Manuscripts for the publication shall be made through the discussion between Developer/Depositor and Recipient investigator.
            Additional terms and conditions:
            Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
            Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Approval form by depositor [Word]
            Remarks ((Additional form)) Approval Form(FormD)
            Remember that you will be working with samples containing infectious virus.
            Please obtain written approval of the depositor.
            提供案内 (日本国内) [open/close]

            提供条件 寄託者に許可をとること。使用にあたっては、常に連絡をとり、発表等に関しても事前に合意を要する。
            付加的提供条件:
            CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            寄託者による提供承諾書 [Word]
            備考 【追加提供依頼書】承諾書(FormD)
            このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
            寄託者の書面による提供承諾が必要です。

            Catalog # Resource name Availability Shipping form Fee (non-profit org.)
            RDB06016 pAdex CAG-SwaI-IRES-EGFP Under QC test. Please contact us. DNA solution

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

            References and tips

            Original reference

            Further references such as user reports and related articles (go to bottom)

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            QC test results

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


            References

            Original, user report and related articles


            2023.04.10

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