Please review the QC test results indicated by 
icon below as well as clone information before placing your order.
提供案内 (日本国内) 
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Please review the QC test results indicated by icon below as well as clone information before placing your order.
icon below as well as clone information before placing your order.
pCMFlag_hsNR1H2 (#RDB06066)
Expression vector of human LXR beta.
| Alternative name | SET-0062_5 |
|---|---|
| Clone info. | Expression vector of human LXR beta. Expression was confirmed by Western blotting with anti-FLAG antibody. |
| Comment | PCR template：HEK293 cells cDNA. dT,Random primer mix. PCR cloning, forward primer: atgtcctctcctaccacgagttc; reverse primer: tcactcgtggacgtcccagatc. |
| Vector backbone | pCMV_S-FLAG (RDB05956) (Plasmid) |
| Size of vector backbone | 5.5 kb |
| Selectable markers | Amp^r (E. coli), Neo^r (mammalian cell) |
| Growth conditions | 37C, LB+Amp |
| Gene/insert name | Human LXR beta cDNA |
| Depositor|Developer | DNA Bank, | |
| Sequence | RDB06066z.seq |
|---|---|
| Remarks, protocol and/or map (pdf) | RDB06066.pdf |
Distribution information
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Ordering forms | Order form [Credit Card Payment  ] [Bank Transfer Payment] [Example of order form ]MTA, for use for not-for-profit academic purpose [Word ] [Example of MTA ]MTA, for use for for-profit purpose [Word ]Please visit Information of Request for Distribution.[link] |
|---|---|
| Terms and conditions for distribution | The RECIPIENT agrees to describe RIKEN BRC as the source of the MATERIAL in any publication. Additional terms and conditions: Regrding pRc/CMV: a) The modified pRc/CMV, and their descendants (collectively, LIFE MATERIALS), should be used for pre-clinical/non-human, research use only. b) LIFE MATERIALS shall not be used for any commercial purposes. c) LIFE MATERIALS shall not be transferred beyond the specified recipient. d) Recipient must contact Thermo Fisher Scientific Inc. at outlicense@thermofisher.com for any desired use of LIFE MATERIALS beyond the scope of a) to c) above. |
[open/close]| 必要書類 | 提供依頼書 [依頼書の記入例 ]提供同意書 (MTA, 非営利学術目的用)[Word ] [MTAの記入例 ]提供同意書 (MTA, 営利目的用)[Word ]手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
|---|---|
| MTAに書く使用条件 | 発表に際し、理研BRCから入手したことを明記すること。 付加的使用条件： pRc/CMVについて: a) The modified pRc/CMV, and their descendants (collectively, LIFE MATERIALS), should be used for pre-clinical/non-human, research use only. b) LIFE MATERIALS shall not be used for any commercial purposes. c) LIFE MATERIALS shall not be transferred beyond the specified recipient. d) Recipient must contact Thermo Fisher Scientific Inc. at outlicense@thermofisher.com for any desired use of LIFE MATERIALS beyond the scope of a) to c) above. |
| Catalog # | Resource name | Shipping form | Fee |
|---|---|---|---|
| RDB06066 | pCMFlag_hsNR1H2 | DNA solution |
JPY 9,460 (not-for-profit academic purpose) JPY 18,920 (for-profit-research purpose) plus cost of shipping containers, dry ice (if required) and shipping charge |
icon below as well as clone information before placing your order.How to cite this biological resource
Materials & Methods section:
| The pCMFlag_hsNR1H2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06066). |
Reference section:
Further references such as user reports and related articles (go to bottom)
QC test results
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
| Test sheet | RDB06066_A3Hr.pdf |
|---|
Nucleotide sequence of a portion of this resource (if available).
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Primer: Sequence file: RDB06066_A3Hra.seq
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>06066_06066_A3Hr_CMV-F_A01_01.ab1 1 TTGTCAACTC CGCCCATTGA CGCAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA 61 AGCAGAGCTC TCTGGCTAAC TAGAGAACCC ACTGCTTACT GGCTTATCGA AATTAATACG 121 ACTCACTATA GGGAGACCCA AGCTGAATTC ACCATGGACT ACAAGGACGA TGACGATAAG 181 GCGGCCATGT CCTCTCCTAC CACGAGTTCC CTGGATACCC CCCTGCCTGG AAATGGCCCC 241 CCTCAGCCTG GCGCCCCTTC TTCTTCACCC ACTGTAAAGG AGGAGGGTCC GGAGCCGTGG 301 CCCGGGGGTC CGGACCCTGA TGTCCCAGGC ACTGATGAGG CCAGCTCAGC CTGCAGCACA 361 GACTGGGTCA TCCCAGATCC CGAAGAGGAA CCAGAGCGCA AGCGAAAGAA GGGCCCAGCC 421 CCGAAGATGC TGGGCCACGA GCTTTGCCGT GTCTGTGGGG ACAAGGCCTC CGGCTTCCAC 481 TACAACGTGC TCAGCTGCGA AGGCTGCAAG GGCTTCTTCC GGCGCAGTGT GGTCCGTGGT 541 GGGGCCAGGC GCTATGCCTG CCGGGGTGGC GGAACCTGCC AGATGGACGC TTTCATGCGG 601 CGCAAGTGCC AGCAGTGCCG GCTGCGCAAG TGCAAGGAGG CAGGGATGAG GGAGCAGTGC 661 GTCCTTTCTG A // |
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Primer: Sequence file: RDB06066_A3Hrb.seq
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>06066_06066_A3Hr_SP6_B01_04.ab1 1 GCTGGGAATC ATGCTCGAGC GGCCTCACTC GTGGACGTCC CAGATCTCCG ACAGCAGAGG 61 CGGCAGCTTC TTGTCCTGGA GCCGCAAGGC GAAGACCTGC TCCGAGTGCA CAGAGCTCAG 121 CGTGCGCAGG CTCACCAGCT TCATGAGCAT GCGCGGGAAG CGCAGCTGGT CCTGCGGCCT 181 CTTGATGCGC GTGTAGGACA GCAGCGCCTC CACGTAGGGC TGCTGCAACG CCTCCACGCG 241 GCCCGGCTCC TGCACGTTGG GCCGGTCGGC CGAGAAGATG TTGATGGCGA TGAGCAGGGC 301 GTACTCAGCG TCGTCCAGGC CCAGCCGCCG CATGGCCCGC GAGAACTCGA AGATGGGGTT 361 GATGAACTCC ACCTGCAGGC CTGCACGGTG GAAGTCGTCC TTGCTGTAGG TGAAGTCCTT 421 CAAGAAGGTG ATACACTCTG TCTCGTGGTT GTAGCGCCTG GCTGTCTCTA GCAGCATGAT 481 CTCGATAGTG GATGCCTTCA GGAGGGCGAT CTGGTCCTCC CGGCCCAGCT GCAGGAAACC 541 AGGCACTTGC TTAGCGAAGT CCACGATCTC CTGGACTGAG ATGATGGCCA GCTCCGTGAA 601 GTGGGCAAAG CGTTGCTGGC GGGCATCTCG GGACTGGGGG TCTGCGCCCA GGGGCCAGGG 661 CGTGACTTTG GGCTGGT // |
Please visit 
Sequencing and PCR primers for primer information.
References
Original, user report and related articles
Resource search
Link
Other resources in our bankExternal Database
Human LXR beta
Reference sequence
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