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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCMFlag_hsNR1H2

Expression vector of human LXR beta.

Catalog number RDB06066
Resource name pCMFlag_hsNR1H2
Alternative name SET-0062_5
Clone info. Expression vector of human LXR beta. Expression was confirmed by Western blotting with anti-FLAG antibody.
Comment PCR template：HEK293 cells cDNA. dT,Random primer mix. PCR cloning, forward primer: atgtcctctcctaccacgagttc; reverse primer: tcactcgtggacgtcccagatc.
Vector backbone pCMV_S-FLAG (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human LXR beta cDNA
Depositor|Developer DNA Bank, |
Other clones in our bank
External Database
Human LXR beta
          Reference sequence
            

          Distribution information

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          RDB06066 pCMFlag_hsNR1H2 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Sequence RDB06066z.seq
          Remarks, protocol and/or map (pdf) RDB06066.pdf

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content pCMV_tag and pRSV_tag assay-ready clones (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB06066_A3Hr.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer:
          Sequence file: RDB06066_A3Hra.seq check
          >06066_06066_A3Hr_CMV-F_A01_01.ab1
              1 TTGTCAACTC CGCCCATTGA CGCAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA
             61 AGCAGAGCTC TCTGGCTAAC TAGAGAACCC ACTGCTTACT GGCTTATCGA AATTAATACG
            121 ACTCACTATA GGGAGACCCA AGCTGAATTC ACCATGGACT ACAAGGACGA TGACGATAAG
            181 GCGGCCATGT CCTCTCCTAC CACGAGTTCC CTGGATACCC CCCTGCCTGG AAATGGCCCC
            241 CCTCAGCCTG GCGCCCCTTC TTCTTCACCC ACTGTAAAGG AGGAGGGTCC GGAGCCGTGG
            301 CCCGGGGGTC CGGACCCTGA TGTCCCAGGC ACTGATGAGG CCAGCTCAGC CTGCAGCACA
            361 GACTGGGTCA TCCCAGATCC CGAAGAGGAA CCAGAGCGCA AGCGAAAGAA GGGCCCAGCC
            421 CCGAAGATGC TGGGCCACGA GCTTTGCCGT GTCTGTGGGG ACAAGGCCTC CGGCTTCCAC
            481 TACAACGTGC TCAGCTGCGA AGGCTGCAAG GGCTTCTTCC GGCGCAGTGT GGTCCGTGGT
            541 GGGGCCAGGC GCTATGCCTG CCGGGGTGGC GGAACCTGCC AGATGGACGC TTTCATGCGG
            601 CGCAAGTGCC AGCAGTGCCG GCTGCGCAAG TGCAAGGAGG CAGGGATGAG GGAGCAGTGC
          661 GTCCTTTCTG A
          //
          Primer:
          Sequence file: RDB06066_A3Hrb.seq check
          >06066_06066_A3Hr_SP6_B01_04.ab1
              1 GCTGGGAATC ATGCTCGAGC GGCCTCACTC GTGGACGTCC CAGATCTCCG ACAGCAGAGG
             61 CGGCAGCTTC TTGTCCTGGA GCCGCAAGGC GAAGACCTGC TCCGAGTGCA CAGAGCTCAG
            121 CGTGCGCAGG CTCACCAGCT TCATGAGCAT GCGCGGGAAG CGCAGCTGGT CCTGCGGCCT
            181 CTTGATGCGC GTGTAGGACA GCAGCGCCTC CACGTAGGGC TGCTGCAACG CCTCCACGCG
            241 GCCCGGCTCC TGCACGTTGG GCCGGTCGGC CGAGAAGATG TTGATGGCGA TGAGCAGGGC
            301 GTACTCAGCG TCGTCCAGGC CCAGCCGCCG CATGGCCCGC GAGAACTCGA AGATGGGGTT
            361 GATGAACTCC ACCTGCAGGC CTGCACGGTG GAAGTCGTCC TTGCTGTAGG TGAAGTCCTT
            421 CAAGAAGGTG ATACACTCTG TCTCGTGGTT GTAGCGCCTG GCTGTCTCTA GCAGCATGAT
            481 CTCGATAGTG GATGCCTTCA GGAGGGCGAT CTGGTCCTCC CGGCCCAGCT GCAGGAAACC
            541 AGGCACTTGC TTAGCGAAGT CCACGATCTC CTGGACTGAG ATGATGGCCA GCTCCGTGAA
            601 GTGGGCAAAG CGTTGCTGGC GGGCATCTCG GGACTGGGGG TCTGCGCCCA GGGGCCAGGG
          661 CGTGACTTTG GGCTGGT
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          2022.05.18

          GNP_filter3_RDBDEP_html_220516.pl