Please review the QC test results indicated by 
icon below as well as clone information before placing your order.
提供案内 (日本国内) 
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Please review the QC test results indicated by icon below as well as clone information before placing your order.
icon below as well as clone information before placing your order.
pCMFlag_hsRORB (#RDB06069)
Expression vector of human ROR beta.
| Alternative name | SET-0092_26 |
|---|---|
| Clone info. | Expression vector of human ROR beta. Expression was confirmed by Western blotting with anti-FLAG antibody. |
| Comment | PCR template：HEK293 cells cDNA. dT,Random primer mix. PCR cloning, forward primer: atgcgagcacaaattgaagtgat; reverse primer: tcatttgcagccggtggcac. |
| Vector backbone | pCMV_S-FLAG (RDB05956) (Plasmid) |
| Size of vector backbone | 5.5 kb |
| Selectable markers | Amp^r (E. coli), Neo^r (mammalian cell) |
| Growth conditions | 37C, LB+Amp |
| Gene/insert name | Human ROR beta cDNA |
| Depositor|Developer | DNA Bank, | |
| Sequence | RDB06069z.seq |
|---|---|
| Remarks, protocol and/or map (pdf) | RDB06069.pdf |
Distribution information
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Ordering forms | Order form [Credit Card Payment  ] [Bank Transfer Payment] [Example of order form ]MTA, for use for not-for-profit academic purpose [Word ] [Example of MTA ]MTA, for use for for-profit purpose [Word ]Please visit Information of Request for Distribution.[link] |
|---|---|
| Terms and conditions for distribution | The RECIPIENT agrees to describe RIKEN BRC as the source of the MATERIAL in any publication. Additional terms and conditions: Regrding pRc/CMV: a) The modified pRc/CMV, and their descendants (collectively, LIFE MATERIALS), should be used for pre-clinical/non-human, research use only. b) LIFE MATERIALS shall not be used for any commercial purposes. c) LIFE MATERIALS shall not be transferred beyond the specified recipient. d) Recipient must contact Thermo Fisher Scientific Inc. at outlicense@thermofisher.com for any desired use of LIFE MATERIALS beyond the scope of a) to c) above. |
[open/close]| 必要書類 | 提供依頼書 [依頼書の記入例 ]提供同意書 (MTA, 非営利学術目的用)[Word ] [MTAの記入例 ]提供同意書 (MTA, 営利目的用)[Word ]手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
|---|---|
| MTAに書く使用条件 | 発表に際し、理研BRCから入手したことを明記すること。 付加的使用条件： pRc/CMVについて: a) The modified pRc/CMV, and their descendants (collectively, LIFE MATERIALS), should be used for pre-clinical/non-human, research use only. b) LIFE MATERIALS shall not be used for any commercial purposes. c) LIFE MATERIALS shall not be transferred beyond the specified recipient. d) Recipient must contact Thermo Fisher Scientific Inc. at outlicense@thermofisher.com for any desired use of LIFE MATERIALS beyond the scope of a) to c) above. |
| Catalog # | Resource name | Shipping form | Fee |
|---|---|---|---|
| RDB06069 | pCMFlag_hsRORB | DNA solution |
JPY 9,460 (not-for-profit academic purpose) JPY 18,920 (for-profit-research purpose) plus cost of shipping containers, dry ice (if required) and shipping charge |
icon below as well as clone information before placing your order.How to cite this biological resource
Materials & Methods section:
| The pCMFlag_hsRORB was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06069). |
Reference section:
Further references such as user reports and related articles (go to bottom)
QC test results
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
| Test sheet | RDB06069_A3Ir.pdf |
|---|
Nucleotide sequence of a portion of this resource (if available).
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Primer: Sequence file: RDB06069_A3Ira.seq
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>06069_06069_A3Ir_2_CMV-Foward_E10_13.ab1 1 ACTAAAAACC TCCCGCCCCA TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT 61 ATATAAGCAG AGCTCTCTGG CTAACTAGAG AACCCACTGC TTACTGGCTT ATCGAAATTA 121 ATACGACTCA CTATAGGGAG ACCCAAGCTG AATTCACCAT GGACTACAAG GACGATGACG 181 ATAAGGCGGC CATGCGAGCA CAAATTGAAG TGATACCATG CAAAATTTGT GGCGATAAGT 241 CCTCTGGGAT CCACTACGGA GTCATCACAT GTGAAGGCTG CAAGGGATTC TTTAGGAGGA 301 GCCAGCAGAA CAATGCTTCT TATTCCTGCC CAAGGCAGAG AAACTGTTTA ATTGACAGAA 361 CGAACAGAAA CCGTTGCCAA CACTGCCGAC TGCAGAAGTG TCTTGCCCTA GGAATGTCAA 421 GAGATGCTGT GAAGTTTGGG AGGATGTCCA AGAAGCAAAG GGACAGCCTG TATGCTGAGG 481 TGCAGAAGCA CCAGCAGCGG CTGCAGGAAC AGCGGCAGCA GCAGAGTGGG GAGGCAGAAG 541 CCCTTGCCAG GGTGTACAGC AGCAGCATTA GCAACGGCCT GAGCAACCTG AACAACGAGA 601 CCAGCGGCAC TTATGCCAAC GGGCACGTCA TTGACCTGCC CAAGTCTGAG GGTTATTACA 661 ACGTCGATTC CGG // |
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Primer: Sequence file: RDB06069_A3Irb.seq
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>06069_06069_A3Ir_2_Sp6_F10_16.ab1 1 GTAGAAATCA ATGCTTCGAA GCCGGCCTCA TTTGCAGCCG GTGGCACAGT CAGGATTAAA 61 GAGCTCCTTG TATAACGGAG GAAACAGTGT ATTCACTATC TCTGGATGAG ATTGCTTAAA 121 TACCTGCAGC TTCTCCCCGT GCAAGTTGCA AACTGCCGTG ATGGTTGGTA TCTTGGCTAT 181 TAACTTTGCC AAGGTCTCAT CATCCAGGTG ATTCTTCTGA ATCACATGTT GAAGTGCAAA 241 ATAAATTTTT TCCTGAAGCT TCTGGACTTT CCTTGGTTCT ATAAGCCAGG CTCGGTCTGG 301 AGATATCAGA ACAGCAGATG AGAACAAAGC GATCTCCTCC TCGGTCAGCT GCAAGGAACA 361 CAAATTCTTT GCAAAGTCAA ATGCTTCATT CACTAGGTCA TCAGAACCTA AGGCTTTGAA 421 CATTTGCATT CCTCCATATT TTCCTTCAAA CAGAACAGTG TTGTTTAATG GGTTGAAGGC 481 ACGGCACATT CTCACTAAAA CCACTTCCAA GCAACCTGAC TTCAGAAGTA GAATTTGATC 541 ATTTTGACAG AGCTCCATGA AGCCTGTTAT CCGCTTTGCA AACTCCACCA CGTATTGGAT 601 GGCGTGAGTG ATCTGGATGG CACATTGTTG CCACAGTGCT TCCCTGGACT TGCTTTGATA 661 TGCTTTAATT TCTTCATAGG T // |
Please visit 
Sequencing and PCR primers for primer information.
References
Original, user report and related articles
Resource search
Link
Other resources in our bankExternal Database
Human ROR beta
Reference sequence
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