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pDUAL-YFH41c (#RDB06152)

S. pombe 3 ter end YFP-FLAG-His6-tagged vector with nmt1 (medium strength) promoter

Clone info. S. pombe 3 ter end YFP-FLAG-His6-tagged vector with nmt1 (medium strength) promoter. Multicopy marker, ura4+; Integration marker, leu1+.
Comment Gateway compatible.
Vector backbone pDUAL (RDB06178) (Plasmid)
Selectable markers E. coli: Amp^r, Cm^r, ccdB.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Reference of insert sequence AB364105.1 (DDBJ accession number)
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
 
Sequence RDB06152z.seq

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature(s). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must state an acknowledgment to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
Remarks Use Survival2 (Invitrogen) or equivalent to amplify this clone.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献の引用と、寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
備考 このクローンの増殖にはSurvival2 (Invitrogen) あるいは同等の宿主を使用してください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06152 pDUAL-YFH41c DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pDUAL-YFH41c was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06152).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13464_A5G7p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw
Sequence file: RDB13464_A5G7a.seq check
>06152_13464_A5G7_nmt1Fw_A10_01.ab1
    1 TGGAGTTATG GAATCAGGAA AACGTAACTC TCGGCTACTG GATGGTTCAG TCACCCAACG
   61 ATTACTGGGG AGAGAAAACA GGGCAAAAGC AAAGCTTAAA GGAATCCGAT TGTCATTCGG
  121 CAATGTGCAG CGAAACTAAA AACCGGATAA TGGACCTGTT AATCGAAACA TTGAAGATAA
  181 AGGAAGAGGA ATCCTGGCAT ATCATCAATT GAATAAGTTG AATTAATTAT TTCAATCTCA
  241 TTCTCACTTT CTGACTTATA GTCGCTTTGT TAAAGCTAGC GTCGACAGGC CTGGATCCGG
  301 ATATCAACAA GTTTGTACAA AAAAGCTGAA CGAGAAACGT AAAATGATAT AAATATCAAT
  361 ATATTAAATT AGATTTTGCA TAAAAAACAG ACTACATAAT ACTGTAAAAC ACAACATATC
  421 CAGTCACTAT GGCGGCCGCA TTAGGCACCC CAGGCTTTAC ACTTTATGCT TCCGGCTCGT
  481 ATAATGTGTG GATTTTGAGT TAGGATCCGT CGAGATTTTC AGGAGCTAAG GAAGCTAAAA
  541 TGGAGAAAAA AATCACTGGA TATACCACCG TTGATATATC CCAATGGCAT CGTAAAGAAC
  601 ATTTTGAGGC ATTTCAGTCA GTTGCTCAAT GTACCTATAA CCAGACCGTT CAGCTGGATA
  661 TTACGGCCTT TTTAAAGACC GTAAAGAAAA ATAAGCACAA GTTTTATCCG GCCTTTATTC
  721 ACATTCTTGC CCGCCTGATG AATGCTCATC CGGAATTCCG TATGGCAATG AAAGACGGTG
  781 AGCTGGTGAT ATGGGATAGT GTTCACCCTT GTTACACCGT TTTCCATGAG CAAACTGAAA
  841 CGTTTTCATC GCTCTGGAGT GAATACCACG ACGATATCCG GCAGTTTCTA CACATATATT
  901 CGCAAGATGT GGCGTGTTAC GGTGAAAACC TGGCCTATTT CCCTAAAGGG TTTATTGAGA
  961 ATATGTTTTT TCGTCTCAGC CCAATCCCTG GTGAGTTTCA CCAGTTTTGA TTTAAACGTG
 1021 GGCCAATATG GGACACTTCT TCGCCCCCGG TTTCACATGG GCAATATATA CGCAAGGCCG
1081 ACAGTGCTGA TGGCCCGCTT GGGCGATTCC AGGCTTTCCA T
//
Primer: YFP_R
Sequence file: RDB13464_A5G7b.seq check
>06152_13464_A5G7_YFP_R1_B10_04.ab1
    1 GAGCTGCATG TCTCGCCGGA ACGCTGACTT GTGGCCGTTT ACGTCGCCGT CCAGCTCGAC
   61 CAGGATGGGC ACCACCCCGG TGAACAGCTC CTCGCCCTTG CTCACCATGA TATCAACCAC
  121 TTTGTACAAG AAAGCTGAAC GAGAAACGTA AAATGATATA AATATCAATA TATTAAATTA
  181 GATTTTGCAT AAAAAACAGA CTACATAATA CTGTAAAACA CAACATATCC AGTCACTATG
  241 GTCGACCTGC AGACTGGCTG TGTATAAGGG AGCCTGACAT TTATATTCCC CAGAACATCA
  301 GGTTAATGGC GTTTTTGATG TCATTTTCGC GGTGGCTGAG ATCAGCCACT TCTTCCCCGA
  361 TAACGGAGAC CGGCACACTG GCCATATCGG TGGTCATCAT GCGCCAGCTT TCATCCCCGA
  421 TATGCACCAC CGGGTAAAGT TCACGGGAGA CTTTATCTGA CAGCAGACGT GCACTGGCCA
  481 GGGGGATCAC CATCCGTCGC CCGGGCGTGT CAATAATATC ACTCTGTACA TCCACAAACA
  541 GACGATAACG GCTCTCTCTT TTATAGGTGT AAACCTTAAA CTGCATTTCA CCAGCCCCTG
  601 TTCTCGTCAG CAAAAGAGCC GTTCATTTCA ATAAACCGGG CGACCTCAGC CATCCCTTCC
  661 TGATTTTCCG CTTTCCAGCG TTCGGCACGC AGACGACGGG CTTCATTCTG CATGGTTGTG
  721 CTTACCAGAC CGGAGATATT GACATCATAT ATGCCTTGAG CAACTGATAG CTGTCGCTGT
  781 CAACTGTCAC TGTAATACGC TGCTTCATAG CATACCTCTT TTTGACATAC TTCGGGTATA
  841 CATATCAGTA TATATTCTTA TACCGCAAAA ATCAGCGCGC AAATACGCAT ACTGTTATCT
  901 GGCTTTTAGT AAGCCGGATC CAGATCTTTA CGCCCCGCCC TGCCACTCAT CGCAGTACTG
  961 TTGTAATTCA TTAAGCATTC TGCCGACATG GAAAGCCATC ACAAACGGCA TGATGAAACC
 1021 TGAATCGCCC AGCGGGCATC AGCACCCTTG TCGCCTTTGC GTATATATTT TGCCCAATGA
1081 TGAAACCGGG GGCCGCAGAG TTGATCCATT ATTTGACCCA CCGGATTTA
//
Primer: ADH_rev
Sequence file: RDB13464_A5G7c.seq check
>06152_13464_A5G7_ADHrev_C10_07.ab1
    1 TGAGAATACT CAAGATAAGA TTTTCGTTTT AAAACCTAAG AGTCACTTTA AAATTTGTAT
   61 ACACTTATTT TTTTTATAAC TTATTTAATA ATAAAAATCA TAAATCATAA GAAATTCGCT
  121 TATTTAGAAG TGGCGCGCCG GATCCTCTAG AGTCGAGATC TCAGTGGTGA TGGTGGTGAT
  181 GTTTGTCATC GTCGTCCTTG TAGTCCATGG GCTTGTACAG CTCGTCCATG CCGAGAGTGA
  241 TCCCGGCGGC GGTCACGAAC TCCAGCAGGA CCATGTGATC GCGCTTCTCG TTGGGGTCTT
  301 TGCTCAGGGC GGACTGGTAG CTCAGGTAGT GGTTGTCGGG CAGCAGCACG GGGCCGTCGC
  361 CGATGGGGGT GTTCTGCTGG TAGTGGTCGG CGAGCTGCAC GCTGCCGTCC TCGATGTTGT
  421 GGCGGATCTT GAAGTTCACC TTGATGCCGT TCTTCTGCTT GTCGGCCATG ATATAGACGT
  481 TGTGGCTGTT GTAGTTGTAC TCCAGCTTGT GCCCCAGGAT GTTGCCGTCC TCCTTGAAGT
  541 CGATGCCCTT CAGCTCGATG CGGTTCACCA GGGTGTCGCC CTCGAACTTC ACCTCGGCGC
  601 GGGTCTTGTA GTTGCCGTCG TCCTTGAAGA AGATGGTGCG CTCCTGGACG TAGCCTTCGG
  661 GCATGGCGGA CTTGAAGAAG TCGTGCTGCT TCATGTGGTC GGGGTAGCGG GCGAAGCACT
  721 GCAGGCCGTA GCCGAAGGTG GTCACGAGGG TGGGCCAGGG CACGGGCAGC TTGCCGGTGG
  781 TGCAGATGAA CTTCAGGGTC AGCTTGCCGT AGGTGGCATC GCCCTCGCCC TCGCCGGACA
  841 CGCTGAACTT GTGGCCGTTT ACGTCGCCGT CCAGCTCGAC CAGGATGGGC ACCACCCCCG
  901 GTGAACAGCT CCTCGCCCTT GCTCACCATG ATATCAACCA CTTTTGTACA AGAAAAGCTG
  961 AAACGAGAAA CGTAAAAATG AATAATAAAT ATCAATATAT TAAAATTTAG AATTTTTGCA
 1021 TAAAAAAAAC CAGACTACCA TAATAACTGT TAAAAACACA AACAATATCC AGTTCACTAT
1081 GATCGGACCC TTGCCAGAAC TGGCTTGTGT CTTATAATTA ACG
//
Primer: ADH-Forward
Sequence file: RDB13464_A5G7d.seq check
>06152_13464_A5G7_ADH_Forward_D10_10.ab1
    1 AGCTCCGTAT TGACACACCT CTACCGGCAG ATCCGCTAGG GATAACAGGG TAATATACTA
   61 GTAAGATGAT TCTTTTATCG TCGATAACGA TGAGGATGTA GACGATTATG ATGAGAGTGA
  121 TTAGCTTCTA AAATTTGGTG TTTTCAAACG CTATTTACAG TACAATCTGA AAAAATTGGA
  181 TGAACATTGT AAATGGTAGG ATACGAGTTC CAATTAATGT ATTTTTTAAG TATTACACTT
  241 AGCTTATTAA TTTTATATAT ACATTCTACC TCTGTGGGTT TAAAGACAAC TCGTACACAT
  301 ACTCAAATTA TTTATGAACA TTATAATGCA TATTCGTACT GTCTATTAAA CATTTTTTTG
  361 ACATTAAAAA TTTCGTTTAC TAACGTAGAA AGCGCTGATA ATTGAGGCTA CTATTTTAAT
  421 ATCTAGAAAC AAAGATTCAT TAATTAATTA AGTAAGCTCC AGGATACTTG TATATTTCGT
  481 TAAAAAAGCG CAATTTCAAC AATTCCTATG AACATCCATG ATTTCATCAC AAAATCAAAC
  541 ATATCAGGAT TTAACGTGAA TTATTTGCTT TCTTGTGATT TTCATCTGAT TTCTGGTCAT
  601 TTACGTTACT GTAAACAAGC TTTTTAAAAA AAGGATTTTT TACGCAATAT TATCGATATC
  661 CCAATCTGTA GTATAAAAAG TGCAACAGTG TTGAGTTTCC CGAAACCAGG CATTTTTGCG
  721 GTAGGGTCTG AAACTTTCAC GAAACCATTT CACAACAAAA CTTCTTCAAC AACATGTGTG
  781 CAAAGAAAAT CGTCGTCTTA CCAGGAGACC ATATTGGCCC TGAAATTGTT GCTTCTGCCT
  841 TGGAGGTTTT GAAAGTCGTT GAGAAGAAGC GACCTGAGTT AAAACTCGAG TTTGAAGAAC
  901 ACAAGATTGG AGGTGCCTCT ATTGATGCCT ATGGAACCCC TTTGACTGAC GAGACTGTGA
  961 AAGGCTTGTT TGGAAGCTGA CGGTGTTCTT TTGGGTGCCG TTGGTGGTCC TGAATGGACC
 1021 CAACCCCATT GTTCGTCTGA AGCACGTTTT AATGGAGGCT CCGTTAGAAG TATGGGTGTT
1081 CGGCCAACCA TCGACCATGC CACCTTTGCC AGCCAAGTTC GATTTAG
//
Primer: ars_R1
Sequence file: RDB13464_A5G7e.seq check
>06152_13464_A5G7_ars_R1_E10_13.ab1
    1 TGGTTCGGTT TATACTATTA CTTAAGTACC TTTAACTAAA CAAAATGCCT ATATATATAT
   61 TAATTTACAA TGAGTGTCAG ATAAGTCACT ATGTCCGAGT GGTTAAGGAG TTAGACTCGA
  121 ATTCGCAAGC TTAGCTACAA ATCCCACTGG CTATATGTAT GCATTTGTGT TAAAAAAGTT
  181 TGTATAGATT ATTTAATCTA CTCAGCATTC TTTCTCTAAA TAGGAATTTG TTACTTAATG
  241 GAGAAAAAAA TGTTTCGATT TACCTAGTGT ATTTGTTTGT ATACTCACGT TTAATTTCAA
  301 ACATCCATTC TATCTTGTGT AATTTTTGGC ATGGTGAAAA AGATAATCAG CCTTATAATC
  361 TTTACAAAAG TAAGAAATTC TGTAAATAAG CCTTAATGCC CTTGCTTTAA ATTAAAATGG
  421 TTCTTTTTCA TGATAATGTT TGCACTTTGT GAATATATTT TAGATAGTTC TGTGAGGTAT
  481 AATTAAGATG TTTTAGAGAC TTATACAATT TTGTCTTTAT AAATTCTTAA TTGATTTTAC
  541 CATCCCAGTT TAACTATGCT TCGTCGGCAT CTCTGCACAT GTCGTGTTTT CTTACCGTAT
  601 TGTCCTACCA AGAACCTCTT TTTTGCTTGG ATCGAAATTA AAGGTTTAAA AGCAAAGTTA
  661 TGGATGCTAG AGTATTTCAA AGCTATTCAG CTAGAGCTGA GGGGATGAAA AATCCCATTG
  721 CCAAGGAATT GTTGGCTTTG ATGGAAGAAA AGCAAAGCAA CTTGTCAGTC GCGGTCGATT
  781 TGACGAAGAA ATCCGAAATC TTAGAATTGG TAGATAAAAT TGGACCCTAT GTCTGTGTTA
  841 TCAAGACACA TATTGACGTT GTCGAGGATT TCGACCAGGA TATGGTAGAA AAACTGGTGG
  901 CCTTAGTAAA AAGCATCGTT TTCTTATCTT TGAGGATCGC AAATTCGCAG ACATTGGAAA
  961 TACCGTCAAG CTACAATATG CATCTGGGTG TGTACAAAAT TTGCTTCTTG GGCTCATATC
 1021 ACAATTTGCC ATACAGTGCC CAGCGAGGGG TATTATTACC AAGTCCTCAA AAGAAGGTTG
1081 ATTAACCGTT GGACGTTGAT CTCTTGGCTT TTTGGCTGGA AATGTTCCTT TCCAAG
//
Primer: f1ori_R3
Sequence file: RDB13464_A5G7f.seq check
>06152_13464_A5G7_f1ori_R3_F10_16.ab1
    1 GAATGGGTCT TGCGTATGCG GTGTGAATAC CGCACAGATG CGTAAGGAGA AAATACCGCA
   61 TCAGGCGCCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT
  121 CTTCGCTATT ACGCCAGCTG GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA
  181 CGCCAGGGTT TTCCCAGTCA CGACGTTGTA AAACGACGGC CAGTGAATTC GAGCTCGGGA
  241 GCGCTACCGT GAATGGGCTC AACCAAACAA TGAACCTTTT CTTCTGATTT TCCTACCACA
  301 CCGGAAAGGG AGGCAGAAGG CAAAAGGCCC AAGCTACCAG GAATGACAGA AGCCTCATCT
  361 GAAATAATGT CACCAAACAA GTTGTCAGTC AAAACAACAC CGTTAAGTGT ACGAGGGCTC
  421 TTGACCAAAA GCATGGCTGC GGAGTCAATG AGCTGGTTTT TTAAGGTAAG GTGAGGATAT
  481 TCCTCCTTAA AAATCTTAGC TACAGTCTTG CGCCAAAGAC GAGAAGTTGC CAAAACATTA
  541 GCTTTGTCGA GTAATGTGAC GGGAGCAGGA GGGTTGGAAG TTTCAGCTAA GCGGCCGCGG
  601 GCCCAACCTT CCAATTCATT AAATCGAGTC CTCCATCAGA TGATTTACAA GGTTTTGCAT
  661 AGAATCCCTA AGTAAGAGAA GTTGTTAGTG ATTTCTTCAA GTTTACTAAA ATTTACATAC
  721 AAATGGATGA TCTCTTCTCC ATTGTTTCCT TTCTTCTTGC AAACGCGTTT TACAAAGAGA
  781 TGACATGCTA TTTTCTGATT ATTTTTTTCT ATAGTTTTCT ATTTTGTACG TCCTTGGGAG
  841 TCCGAAATGT AAAATCGGGG GTATGGTTAG TAGCGGCAGC GTTCATTATG GTATATAAAA
  901 GATAAAAAGT AGGGGGCAAG CGAGACAGAA TGGGATACAA GGGCATCGTC TATAATTATA
  961 GCTAAAAATT GTATTTTAAT TTGTATTTTT TTTGTAATTT TATTTCTCAT TTGTTTTACT
 1021 TTAAAATGAT GCGAAATTTA GAGAAAACTT CACCGAAATG TCCAAAATAA GCCTCAGCAC
1081 ATATACAAGT TTTAGGAAAG CGATCAGCAA CCTTTAA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

user_report Zhang, M.M., Quantitative control of protein S-palmitoylation regulates meiotic entry in fission yeast. PLoS Biol., 11 (7): e1001597 (2013). PMID 23843742. [link to RRC of NBRP]