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pDUAL2-HFF1 (#RDB06193)

S. pombe 5 ter end His6-FLAG2-tagged vector with nmt1 promoter

Clone info. S. pombe 5 ter end His6-FLAG2-tagged vector with nmt1 promoter.
Comment Multicopy marker, ura4+; Integration marker, ura4+, leu1-. Gateway incompatible.
Vector backbone pDUAL2 (RDB06192) (Plasmid)
Selectable markers Amp^r
Reference of insert sequence AB364059.1 (DDBJ accession number)
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
 
Sequence RDB06193z.seq

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、　寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06193 pDUAL2-HFF1 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pDUAL2-HFF1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06193).

Reference section:

Matsuyama, A., Yoshida, M., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913. [link to RRC of NBRP]
Matsuyama, A., Shirai, A., Yashiroda, Y., Kamata, A., Horinouchi, S., Yoshida, M., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14487_A6L7p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw
Sequence file: RDB14487_A6L7a.seq check
>06193_14487_A6L7_nmt1Fw_A09_03_ABI24.ab1
    1 ATGGCATTTT ATTAAATCAG GAAAACGTAA CTCTCGGCTA CTGGATGGTT CAGTCACCCA
   61 ACGATTACTG GGGAGAGAAA ACAGGGCAAA AGCAAAGCTT AAAGGAATCC GATTGTCATT
  121 CGGCAATGTG CAGCGAAACT AAAAACCGGA TAATGGACCT GTTAATCGAA ACATTGAAGA
  181 TATATAAAGG AAGAGGAATC CTGGCATATC ATCAATTGAA TAAGTTGAAT TAATTATTTC
  241 AATCTCATTC TCACTTTCTG ACTTATAGTC GCTTTGTTAA AGCTAGCGTC GACAGGCCTG
  301 GATCCTATGC ATCACCACCA TCATCATATG GATTATAAGG ATGATGACGA TAAACCCATG
  361 GACTACAAGG ACGACGATGA CAAAGATATC ATGAGATCTC GACTCTAGAG GATCCGGCGC
  421 GCCACTTCTA AATAAGCGAA TTTCTTATGA TTTATGATTT TTATTATTAA ATAAGTTATA
  481 AAAAAAATAA GTGTATACAA ATTTTAAAGT GACTCTTAGG TTTTAAAACG AAAATTCTTA
  541 TTCTTGAGTA ACTCTTTCCT GTAGGTCAGG TTGCTTTCTC AGGTATAGTA TGAGGTCGCT
  601 CTTATTGACC ACACCTCTAC CGGCAGATCC GCTAGGGATA ACAGGGTAAT ATACTAGTGC
  661 CTCTATTGAT GCCTATGGAA CCCCTTTGAC TGACGAGACT GTGAAGGCTT GTTTGGAAGC
  721 TGACGGTGTT CTTTTGGGTG CCGTTGGTGG TCCTGAAATG GACCAACCCC CAATTGTCGT
  781 CCTGAGCAAG GTTTATTGAA GCTTCGTAAG AGTATGGGTG TTTGGGCCAA CCTTTCGACC
  841 TTGCAACTTT GCCAGCAAGT CTTTAGTCAA GTACAGCCCT TTTGAAGCCC TGAAATCGTT
  901 GGAAGTGTCG ATTTTTTGTG TTGTACGGAG AACTTACTGG AGTTGTTTAC TTTGTGGGAG
961 CGCCCTTGAG
//
Primer: ADH-Forward
Sequence file: RDB14487_A6L7b.seq check
>06193_14487_A6L7_ADH-Forward_C04_03_ABI08.ab1
    1 TACGGTTTTA TTGAACACAC CTCTACCGGC AGATCCGCTA GGGATAACAG GGTAATATAC
   61 TAGTGCCTCT ATTGATGCCT ATGGAACCCC TTTGACTGAC GAGACTGTGA AGGCTTGTTT
  121 GGAAGCTGAC GGTGTTCTTT TGGGTGCCGT TGGTGGTCCT GAATGGACCA ACCCCAATTG
  181 TCGTCCTGAG CAAGGTTTAT TGAAGCTTCG TAAGAGTATG GGTGTTTGGG CCAACCTTCG
  241 ACCTTGCAAC TTTGCCAGCA AGTCTTTAGT CAAGTACAGC CCTTTGAAGC CTGAAATCGT
  301 TGAAGGTGTC GATTTTTGTG TTGTACGAGA ACTTACTGGA GGTTGTTACT TTGGTGAGCG
  361 CACTGAGGAC AACGGATCGG GTTATGCTAT GGACACTTGG CCTTACAGTT TGGAAGAAGT
  421 TTCTCGTATT GCTCGTTTGG CGGCCGCGGG CCCAACCTTC CAATTCATTA AATCGAGTCC
  481 TCCATCAGAT GATTTACAAG GTTTTGCATA GAATCCCTAA GTAAGAGAAG TTGTTAGTGA
  541 TTTCTTCAAG TTTACTAAAA TTTACATACA AATGGATGAT CTCTTCTCCA TTGTTTCCTT
  601 TCTTCTTGCA AACGCGTTTT ACAAAGAGAT GACATGCTAT TTTCTGATTA TTTTTTTCTA
  661 TAGTTTTCTA TTTTGTACGT CCTTGGGAGT CCGAAATGTA AAATCGGGGT ATGGTTAGTA
  721 GCGGCAGCGT TCATTATGGT ATATAAAAGA TAAAAAGTAG GGGCAAGCGA GACAGAATGG
  781 GATACAAGGG CATCGTCTAT AATTATAGCT AAAAATTGTA TTTTAATTTG TATTTTTTTG
  841 TAATTTTATT TCTCATTGTT TTACTTAAAA TGAAATGCGA AATTAGAGAA AACTTCAACG
  901 AAATGTCAAA ATAAGCTCAG CAAAATATAC AATTTTAGGG AAAGCGATCA GCAACTTTAT
  961 TCTCCGTTGT AGATTCTTTT TCTTCATAAA ATTTCCACAA TGTAACCTGG CCAAATTGAT
 1021 TTTTTTCTTC TAGATGCTTA TACTTGGAAC CTTACTGCAA TATACTAACA CCAGGTTTTG
1081 GCATAAAGTG GTCAC
//
Primer: f1ori_R3
Sequence file: RDB14487_A6L7c.seq check
>06193_14487_A6L7_f1ori_R3_C09_09_ABI24.ab1
    1 GCATGGCGGC TTTGAGTATG CGGTGTGAAT ACCGCACAGA TGCGTAAGGA GAAAATACCG
   61 CATCAGGCGC CATTCGCCAT TCAGGCTGCG CAACTGTTGG GAAGGGCGAT CGGTGCGGGC
  121 CTCTTCGCTA TTACGCCAGC TGGCGAAAGG GGGATGTGCT GCAAGGCGAT TAAGTTGGGT
  181 AACGCCAGGG TTTTCCCAGT CACGACGTTG TAAAACGACG GCCAGTGAAT TCGAGCTCGG
  241 GAGCGCTACC GTGAATGGGC TCAACCAAAC AATGAACCTT TTCTTCTGAT TTTCCTACCA
  301 CACCGGAAAG GGAGGCAGAA GGCAAAAGGC CCAAGCTACC AGGAATGACA GAAGCCTCAT
  361 CTGAAATAAT GTCACCAAAC AAGTTGTCAG TCAAAACAAC ACCGTTAAGT GTACGAGGGC
  421 TCTTGACCAA AAGCATGGCT GCGGAGTCAA TGAGCTGGTT TTTTAAGGTA AGGTGAGGAT
  481 ATTCCTCCTT AAAAATCTTA GCTACAGTCT TGCGCCAAAG ACGAGAAGTT GCCAAAACAT
  541 TAGCTTTGTC GAGTAATGTG ACGGGAGCAG GAGGGTTGGA AGTTTCAGCT AAGCGGCCGC
  601 GGGCCCGAGT CTAACTCCTT AAACCACTCG GACATAGTGA CTTATCTGAC ACTCATTGTA
  661 AATTAATATA TATATAGGCA TTTTTGTTTA GTTAAAGGTA CTTAAGTAAT TTAGTATAAA
  721 CGAACCAATT TTATAATCAG GAAAGTTTAA GTGAATGGGT AGCACATGTC GTAAAAATTG
781 TGGAATTTTT TATTTGAAAT AAAATTTTAA ATACAAAACC TTTTCTAAAA CCAGGTAT
//
Primer: Reverse2
Sequence file: RDB14487_A6L7d.seq check
>06193_14487_A6L7_Reverse2_D09_12_ABI24.ab1
    1 CAGTCCTGAT TACGCCAGCT TGTGATATTG ACGAAACTTT TTGACATCTA ATTTATTCTG
   61 TTCCAACACC AATGTTTATA ACCAAGTTTT ATCTTGTTTG TCTACATGGT ATTTTACATT
  121 CATCTACATA CATCTTTCAT TGGCTTTGTA CATAGTTATC ATTACAAGTC TAAAAAAATT
  181 CACTCTTTTC TTATTCAATG TCAATCCAAG AGAAAAGATT GTGGTAATGT TGTAGGAGCA
  241 TGTTTAATAA ATTACTATAG CAAATTACTT TTTATTCCCA AGGTGTTTAT CTATAATAGT
  301 TAATATTTTA GTCGCTACAT AAAATTTTAC CAAAGAGTAC TTGTATACTA ATTCTAAATG
  361 CCTTCTGACA TAAAACGCCT AGGAAAACAA ACGCAAACAA GGCATCGACT TTTTCAATAA
  421 CCAACCAAAA AAATTTTACA TTAGTCTTTT TTTAATGCTG AGAAAGTCTT TGCTGATATG
  481 CCTTCCAACC AGCTTCTCTA TATCTCTTGG CTTCGACAAC AGGATTACGA CCAGCTCCAT
  541 AGACTCCACG ACCAACAATG ATGATGTCGC TACCGCAGTT TACAATCACT TCTTCAGGAG
  601 TACGATATTG CTGTCCCAGC CCGTCTCCTT TAACATCCAA GCCGATACCA GGGGACATAG
  661 TTATGTAGTC GCTTTGAAGG TTAGGAAATC GACGACCAGC TATAAAGCCA AAGCAAAAAT
  721 CGGTATGCTT CTCAAACCAT TCTAAGGGTT TTCTCTGTGT AGGAACCAGT AGGCCAAAGA
  781 GCCTTTGGGA AGACATTTCA GCCAAAAGGC AAGAGACCAC GTCCCAAAGG TAAAACCAAA
  841 CTTTCTTTGG AAGGCCTTGT ATAATACCCT TCGCCCTGGG CAACTGTATG GGCAATTTGG
  901 TGGAATATGG AGGCCCAAAG AAAGCAATTT TGGTACACAG CAGGATGGCA TATTTGGTAG
  961 GCTTGGACGG GTAATTTTCC ATGGTTCTGG CGAAATTTGG CGGATCCTCA AAGAATAGAA
 1021 ACGGATGGCC TTTACCTTAG CACCAGGTTT TCTACATATC TGGTCGGAAG TCTTCGGAAC
1081 AAAGCGTCCA ATGTGGTGGT CCTTTGA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913. [link to RRC of NBRP]
original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162. [link to RRC of NBRP]