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pDUAL2-HFF41 (#RDB06194)

S. pombe 5 ter end His6-FLAG2-tagged vector with nmt1 (medium strength) promoter

Clone info. S. pombe 5 ter end His6-FLAG2-tagged vector with nmt1 (medium strength) promoter.
Comment Multicopy marker, ura4+; Integration marker, ura4+, leu1-. Gateway incompatible.
Vector backbone pDUAL2 (RDB06192) (Plasmid)
Selectable markers Amp^r
Reference of insert sequence AB364060.1 (DDBJ accession number)
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
 
Sequence RDB06194z.seq

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、　寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06194 pDUAL2-HFF41 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pDUAL2-HFF41 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06194).

Reference section:

Matsuyama, A., Yoshida, M., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913. [link to RRC of NBRP]
Matsuyama, A., Shirai, A., Yashiroda, Y., Kamata, A., Horinouchi, S., Yoshida, M., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14488_A6L7p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw
Sequence file: RDB14488_A6L7a.seq check
>06194_14488_A6L7_nmt1Fw_D04_04_ABI08.ab1
    1 TGAAATTTAA TTTAATCAAG GAAAACGTAA CTCTCGGCTA CTGGATGGTT CAGTCACCCA
   61 ACGATTACTG GGGAGAGAAA ACAGGGCAAA AGCAAAGCTT AAAGGAATCC GATTGTCATT
  121 CGGCAATGTG CAGCGAAACT AAAAACCGGA TAATGGACCT GTTAATCGAA ACATTGAAGA
  181 TAAAGGAAGA GGAATCCTGG CATATCATCA ATTGAATAAG TTGAATTAAT TATTTCAATC
  241 TCATTCTCAC TTTCTGACTT ATAGTCGCTT TGTTAAAGCT AGCGTCGACA GGCCTGGATC
  301 CTATGCATCA CCACCATCAT CATATGGATT ATAAGGATGA TGACGATAAA CCCATGGACT
  361 ACAAGGACGA CGATGACAAA GATATCATGA GATCTCGACT CTAGAGGATC CGGCGCGCCA
  421 CTTCTAAATA AGCGAATTTC TTATGATTTA TGATTTTTAT TATTAAATAA GTTATAAAAA
  481 AAATAAGTGT ATACAAATTT TAAAGTGACT CTTAGGTTTT AAAACGAAAA TTCTTATTCT
  541 TGAGTAACTC TTTCCTGTAG GTCAGGTTGC TTTCTCAGGT ATAGTATGAG GTCGCTCTTA
  601 TTGACCACAC CTCTACCGGC AGATCCGCTA GGGATAACAG GGTAATATAC TAGTGCCTCT
  661 ATTGATGCCT ATGGAACCCC TTTGACTGAC GAGACTGTGA AGGCTTGTTT GGAAGCTGAC
  721 GGTGTTCTTT TGGGTGCCGT TGGTGGTCCT GAATGGACCA ACCCCAATTG TCGTCCTGAG
  781 CAAGGTTTAT TGAAGCTTCG TAAGAGTATG GGTGTTTGGG CCAACCTTCG ACCTTGCAAC
  841 TTTGCCAGCA AGTCTTTAGT CAAGTACAGC CCTTTGAAGC CTGAAATCGT TGAAGTGTCG
  901 ATTTTTGTGT TGTACGAGAA CTTACTGGAG GTTGTTACTT TGGGTGAGCC GCCACTGAGG
  961 ACACGATCGG TTATGCTATG GACCACTTGG CCCTTACAGT TGGAAGAAGT TTCCTCGTAA
 1021 TGGCCTCGAT TTGGCGGCCC GCGGGCCGAC TCCCAATCCA ATTGATCGAG TCGTGATCGA
1081 ATGAATTACA ACGTTTTTGC GACTA
//
Primer: ADH-Forward
Sequence file: RDB14488_A6L7b.seq check
>06194_14488_A6L7_ADH-Forward_E04_05_ABI08.ab1
    1 ATCTCCTTTT AATTGAAACA CCTCTACCGG CAGATCCGCT AGGGATAACA GGGTAATATA
   61 CTAGTGCCTC TATTGATGCC TATGGAACCC CTTTGACTGA CGAGACTGTG AAGGCTTGTT
  121 TGGAAGCTGA CGGTGTTCTT TTGGGTGCCG TTGGTGGTCC TGAATGGACC AACCCCAATT
  181 GTCGTCCTGA GCAAGGTTTA TTGAAGCTTC GTAAGAGTAT GGGTGTTTGG GCCAACCTTC
  241 GACCTTGCAA CTTTGCCAGC AAGTCTTTAG TCAAGTACAG CCCTTTGAAG CCTGAAATCG
  301 TTGAAGGTGT CGATTTTTGT GTTGTACGAG AACTTACTGG AGGTTGTTAC TTTGGTGAGC
  361 GCACTGAGGA CAACGGATCG GGTTATGCTA TGGACACTTG GCCTTACAGT TTGGAAGAAG
  421 TTTCTCGTAT TGCTCGTTTG GCGGCCGCGG GCCCAACCTT CCAATTCATT AAATCGAGTC
  481 CTCCATCAGA TGATTTACAA GGTTTTGCAT AGAATCCCTA AGTAAGAGAA GTTGTTAGTG
  541 ATTTCTTCAA GTTTACTAAA ATTTACATAC AAATGGATGA TCTCTTCTCC ATTGTTTCCT
  601 TTCTTCTTGC AAACGCGTTT TACAAAGAGA TGACATGCTA TTTTCTGATT ATTTTTTTCT
  661 ATAGTTTTCT ATTTTGTACG TCCTTGGGAG TCCGAAATGT AAAATCGGGG TATGGTTAGT
  721 AGCGGCAGCG TTCATTATGG TATATAAAAG ATAAAAAAGT AGGGGCAAGC GAGACAGAAT
  781 GGGATACAAG GGCATCGTCT ATAATTATAG CTAAAAAATT GTATTTTAAT TTGTATTTTT
  841 TGTAAATTTT ATTTCTCATT GTTTTACTTA AAATGAATGC GAATTTAAAG AAAAACTTCA
  901 ACGAAATGGT CAAAATTAAA GCTCCACAAA AATATTACCA ATTTTTAGGG AAAGCCGATT
  961 CAGCAACTTT ATTCTTCCGG TTTGTAGGAT TTCTTTTTTC TTTCAATAAA TTCCAACCAT
 1021 GCTACCTGGC CAATTGAATT CTTTCTCTCT AAATTGCTAT TAATTGAAAC CTAATGGATA
1081 TACTACCACA GCGTTTTTTG GACATTAAAT TGTTATGAAC TAT
//
Primer: f1ori_R3
Sequence file: RDB14488_A6L7c.seq check
>06194_14488_A6L7_f1ori_R3_F04_06_ABI08.ab1
    1 GGCGTAGGGT CCTTGGCAGT ATGCGGGTGT GAATACCGCA CAGATGCGTA AGGAGAAAAT
   61 ACCGCATCAG GCGCCATTCG CCATTCAGGC TGCGCAACTG TTGGGAAGGG CGATCGGTGC
  121 GGGCCTCTTC GCTATTACGC CAGCTGGCGA AAGGGGGATG TGCTGCAAGG CGATTAAGTT
  181 GGGTAACGCC AGGGTTTTCC CAGTCACGAC GTTGTAAAAC GACGGCCAGT GAATTCGAGC
  241 TCGGGAGCGC TACCGTGAAT GGGCTCAACC AAACAATGAA CCTTTTCTTC TGATTTTCCT
  301 ACCACACCGG AAAGGGAGGC AGAAGGCAAA AGGCCCAAGC TACCAGGAAT GACAGAAGCC
  361 TCATCTGAAA TAATGTCACC AAACAAGTTG TCAGTCAAAA CAACACCGTT AAGTGTACGA
  421 GGGCTCTTGA CCAAAAGCAT GGCTGCGGAG TCAATGAGCT GGTTTTTTAA GGTAAGGTGA
  481 GGATATTCCT CCTTAAAAAT CTTAGCTACA GTCTTGCGCC AAAGACGAGA AGTTGCCAAA
  541 ACATTAGCTT TGTCGAGTAA TGTGACGGGA GCAGGAGGGT TGGAAGTTTC AGCTAAGCGG
  601 CCGCGGGCCC GAGTCTAACT CCTTAACCAC TCGGACATAG TGACTTATCT GACACTCATT
  661 GTAAATTAAT ATATATATAG GCATTTTGTT TAGTTAAAGG TACTTAAGTA ATTAGTATAA
  721 ACGAACCAAT TTTATAATCA GGAAGTTAAG TGAATGGTAG CACATGTCGT AAAAATTGTG
  781 AATTTTTATT GAATAATATT TTAAATACAA GCCTTTCTAG ACTAGGTATA CTCATAAACA
  841 TATGAGCAAA AGGATAGAGG AGATTACATT GCATCTTCTA CAAATTTATT TATTGCCCTT
  901 TACTGAAAAA ATTAAATAAT GAGTACTAAA TGATAAAAAG CGCTCAGTAC AAGAAAGATT
  961 GCAAAAATAT TGCATTCTTC ATGAATTTAA AGGTTTGCAT AATAAGGGCA TATTTGAAAG
 1021 TAATAGTACT AAAACAGCCA GGTTAAGCCG AGATATTAGA GTTATTATTC GCAGACATGT
1081 GACCATTAGA TTAAAAAGAC TTTGTAAGAC
//
Primer: Reverse2
Sequence file: RDB14488_A6L7d.seq check
>06194_14488_A6L7_Reverse2_H09_24_ABI24.ab1
    1 CATAAATCAT TACGCCAGCT TGTGATATTG ACGAAACTTT TTGACATCTA ATTTATTCTG
   61 TTCCAACACC AATGTTTATA ACCAAGTTTT ATCTTGTTTG TCTACATGGT ATTTTACATT
  121 CATCTACATA CATCTTTCAT TGGCTTTGTA CATAGTTATC ATTACAAGTC TAAAAAAATT
  181 CACTCTTTTC TTATTCAATG TCAATCCAAG AGAAAAGATT GTGGTAATGT TGTAGGAGCA
  241 TGTTTAATAA ATTACTATAG CAAATTACTT TTTATTCCCA AGGTGTTTAT CTATAATAGT
  301 TAATATTTTA GTCGCTACAT AAAATTTTAC CAAAGAGTAC TTGTATACTA ATTCTAAATG
  361 CCTTCTGACA TAAAACGCCT AGGAAAACAA ACGCAAACAA GGCATCGACT TTTTCAATAA
  421 CCAACCAAAA AAATTTTACA TTAGTCTTTT TTTAATGCTG AGAAAGTCTT TGCTGATATG
  481 CCTTCCAACC AGCTTCTCTA TATCTCTTGG CTTCGACAAC AGGATTACGA CCAGCTCCAT
  541 AGACTCCACG ACCAACAATG ATGATGTCGC TACCGCAGTT TACAATCACT TCTTCAGGAG
  601 TACGATATTG CTGTCCCAGC CCGTCTCCTT TAACATCCAA GCCGATACCA GGGGACATAG
  661 TTATGTAGTC GCTTTGAAGG TTAGGAAATC GACGACCAGC TATAAAGCCA AAGCAAAAAT
  721 CGGTATGCTT TCTCAAACCA TTCTAAGGTT TTCTCTGTGT AGGAAACCAG TAGCCCAAAG
  781 AGCCTTTTGG AAGACATTTC AACCCAAAAG CAAAGAGACC ACGTCCCAAA GGTAAACCAA
  841 CTTTCTTTGA GGCCCTTTGT ATAATACCCC TCGCCCTGGC ACTGTATGGC AATTTTGTGA
  901 TATGAGCCCC AAGAAGCAAT TTTGTACACA CCAGATGCCT ATTTGGTAAC TTTGACCGGT
961 ATTTTCCAAT TGTTCGGCGA AAT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913. [link to RRC of NBRP]
original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162. [link to RRC of NBRP]