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pDUAL2-FFH1 (#RDB06199)

S. pombe 3 ter end FLAG2-His6-tagged vector with nmt1 promoter

Clone info. S. pombe 3 ter end FLAG2-His6-tagged vector with nmt1 promoter.
Comment Multicopy marker, ura4+; Integration marker, ura4+, leu1-. Gateway incompatible.
Vector backbone pDUAL2 (RDB06192) (Plasmid)
Selectable markers Amp^r
Reference of insert sequence AB364065.1 (DDBJ accession number)
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
 
Sequence RDB06199z.seq

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、　寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06199 pDUAL2-FFH1 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pDUAL2-FFH1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06199).

Reference section:

Matsuyama, A., Yoshida, M., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913. [link to RRC of NBRP]
Matsuyama, A., Shirai, A., Yashiroda, Y., Kamata, A., Horinouchi, S., Yoshida, M., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14529_A6L7p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw
Sequence file: RDB14529_A6L7a.seq check
>06199_14529_3_A6L7_nmt1Fw_G04_07_ABI08.ab1
    1 TGAAATTAAT AAATCAGGAA ACGTAACTCT CGGCTACTGG ATGGTTCAGT CACCCAACGA
   61 TTACTGGGGA GAGAAAACAG GGCAAAAGCA AAGCTTAAAG GAATCCGATT GTCATTCGGC
  121 AATGTGCAGC GAAACTAAAA ACCGGATAAT GGACCTGTTA ATCGAAACAT TGAAGATATA
  181 TAAAGGAAGA GGAATCCTGG CATATCATCA ATTGAATAAG TTGAATTAAT TATTTCAATC
  241 TCATTCTCAC TTTCTGACTT ATAGTCGCTT TGTTAAAGCT AGCGTCGACA GGCCTGGATC
  301 CGGATATCAT GGATTATAAG GATGATGACG ATAAACCCAT GGACTACAAG GACGACGATG
  361 ACAAACATCA CCACCATCAC CACTGAGATC TCGACTCTAG AGGATCCGGC GCGCCACTTC
  421 TAAATAAGCG AATTTCTTAT GATTTATGAT TTTTATTATT AAATAAGTTA TAAAAAAAAT
  481 AAGTGTATAC AAATTTTAAA GTGACTCTTA GGTTTTAAAA CGAAAATTCT TATTCTTGAG
  541 TAACTCTTTC CTGTAGGTCA GGTTGCTTTC TCAGGTATAG TATGAGGTCG CTCTTATTGA
  601 CCACACCTCT ACCGGCAGAT CCGCTAGGGA TAACAGGGTA ATATACTAGT GCCTCTATTG
  661 ATGCCTATGG AACCCCTTTG ACTGACGAGA CTGTGAAGGC TTGTTTGGAA GCTGACGGTG
  721 TTCTTTTGGG TGCCGTTGGT GGTCCTGAAT GGACCAACCC CAATTGTCGT CCTGAGCAAG
  781 GTTTATTGAA GCTTCGTAAG AGTATGGGTG TTTGGGCCAA CCTTCGACCT TGCAACTTTG
  841 CCAGCAAGTC TTTAGTCAAG TACAGCCCTT TGAAGCCTGA AATCGTTGAA GGTGTCGATT
  901 TTTTGTGTTG TACGAGAACT TTACTGGAGG TTGTTACTTT GGGTGAGCGC ACTGAGGACA
  961 ACGGGATCGG GGTTATGCTA TGGACACTTT GGTCCTTTAC AGTTTGGCAA GGAAGTTTCT
 1021 CGTATGCTCG TTTGCGCCGC GACCATCCTT CCAATTCATT AATCGAGTTC GTCATCAGAT
1081 GATTTTAGAC GGAT
//
Primer: ADH-Forward
Sequence file: RDB14529_A6L7b.seq check
>06199_14529_3_A6L7_ADH-Forward_H04_08_ABI08.ab1
    1 ACCTCTCTTA TTGACACACC TCTACCGGCA GATCCGCTAG GGATAACAGG GTAATATACT
   61 AGTGCCTCTA TTGATGCCTA TGGAACCCCT TTGACTGACG AGACTGTGAA GGCTTGTTTG
  121 GAAGCTGACG GTGTTCTTTT GGGTGCCGTT GGTGGTCCTG AATGGACCAA CCCCAATTGT
  181 CGTCCTGAGC AAGGTTTATT GAAGCTTCGT AAGAGTATGG GTGTTTGGGC CAACCTTCGA
  241 CCTTGCAACT TTGCCAGCAA GTCTTTAGTC AAGTACAGCC CTTTGAAGCC TGAAATCGTT
  301 GAAGGTGTCG ATTTTTGTGT TGTACGAGAA CTTACTGGAG GTTGTTACTT TGGTGAGCGC
  361 ACTGAGGACA ACGGATCGGG TTATGCTATG GACACTTGGC CTTACAGTTT GGAAGAAGTT
  421 TCTCGTATTG CTCGTTTGGC GGCCGCGGGC CCAACCTTCC AATTCATTAA ATCGAGTCCT
  481 CCATCAGATG ATTTACAAGG TTTTGCATAG AATCCCTAAG TAAGAGAAGT TGTTAGTGAT
  541 TTCTTCAAGT TTACTAAAAT TTACATACAA ATGGATGATC TCTTCTCCAT TGTTTCCTTT
  601 CTTCTTGCAA ACGCGTTTTA CAAAGAGATG ACATGCTATT TTCTGATTAT TTTTTTCTAT
  661 AGTTTTCTAT TTTGTACGTC CTTGGGAGTC CGAAATGTAA AATCGGGGTA TGGTTAGTAG
  721 CGGCAGCGTT CATTATGGTA TATAAAAGAT AAAAAGTATG GGCAAGCGAG ACAGAATGGG
  781 ATACAAAGGG CATCGTCTAT AATTATAGCT AAAAATTGTA TTTTAATTTG TATTTTTTGT
  841 AATTTTAATT TCTCATTGTT TTACTTAAAA TGAATGCGAA TTAGAGAAAA ACTTTCACGA
  901 ATGTCAAAAT AAGCTTCAGC AAAAATATAA CAATTTTAGG AAAGGCGATC AGCCACTTAT
  961 TCTCGGTGGT AGATTCCTTT TCCTCCATAA TTCGAACAAT GTACTGGCCG ATGGATTTTC
1021 TCTTAGAATG CCTAATAATT GGTACTATGA TGATAGCCAG TTGATAG
//
Primer: f1ori_R3
Sequence file: RDB14529_A6L7c.seq check
>06199_14529_3_A6L7_f1ori_R3_A05_01_ABI08.ab1
    1 GCCGATGGGT GTCCTTTGCG TATGCGGTGT GAATACCGCA CAGATGCGTA AGGAGAAAAT
   61 ACCGCATCAG GCGCCATTCG CCATTCAGGC TGCGCAACTG TTGGGAAGGG CGATCGGTGC
  121 GGGCCTCTTC GCTATTACGC CAGCTGGCGA AAGGGGGATG TGCTGCAAGG CGATTAAGTT
  181 GGGTAACGCC AGGGTTTTCC CAGTCACGAC GTTGTAAAAC GACGGCCAGT GAATTCGAGC
  241 TCGGGAGCGC TACCGTGAAT GGGCTCAACC AAACAATGAA CCTTTTCTTC TGATTTTCCT
  301 ACCACACCGG AAAGGGAGGC AGAAGGCAAA AGGCCCAAGC TACCAGGAAT GACAGAAGCC
  361 TCATCTGAAA TAATGTCACC AAACAAGTTG TCAGTCAAAA CAACACCGTT AAGTGTACGA
  421 GGGCTCTTGA CCAAAAGCAT GGCTGCGGAG TCAATGAGCT GGTTTTTTAA GGTAAGGTGA
  481 GGATATTCCT CCTTAAAAAT CTTAGCTACA GTCTTGCGCC AAAGACGAGA AGTTGCCAAA
  541 ACATTAGCTT TGTCGAGTAA TGTGACGGGA GCAGGAGGGT TGGAAGTTTC AGCTAAGCGG
  601 CCGCGGGCCC GAGTCTAACT CCTTAACCAC TCGGACATAG TGACTTATCT GACACTCATT
  661 GTAAATTAAT ATATATATAG GCATTTTGTT TAGTTAAAGG TACTTAAGTA ATTAGTATAA
  721 ACGAACCAAT TTTATAATCA GGAAGTTAAG TGAATGGTAG CACATGTCGT AAAAATTGTG
  781 AATTTTTATT GAATAATATT TTAAATACAA GCCTTTCTAG ACTAGGTATA CTCATAAACA
  841 TATGAGCAAA AGGATAGAGG AGATTACATT GCATCTTCTA CAAATTTATT TATTGCCCTT
  901 TACTGAAAAA TTAAATAATG AGTACTAAAT GATAAAAAAG CGCTCAGTAC AAGAAAGATT
  961 GCAAAAATAT TGCATTCTTT CATGAATTAA GTTGGCATAT AAGCATAATT GAAGGTTAAT
 1021 AGTTACTAAA ACAGCAGTTA GCCGAAATAT AGGATTAATA TTCGCAGAAA TTGTGCACAT
1081 AAGTTGAAGG AAATGTGTAC GA
//
Primer: Reverse2
Sequence file: RDB14529_A6L7d.seq check
>06199_14529_3_A6L7_Reverse2_B05_02_ABI08.ab1
    1 AGGGCCATGC ATTCGCCAAG CTTGTGATAT TGACGAAACT TTTTGACATC TAATTTATTC
   61 TGTTCCAACA CCAATGTTTA TAACCAAGTT TTATCTTGTT TGTCTACATG GTATTTTACA
  121 TTCATCTACA TACATCTTTC ATTGGCTTTG TACATAGTTA TCATTACAAG TCTAAAAAAA
  181 TTCACTCTTT TCTTATTCAA TGTCAATCCA AGAGAAAAGA TTGTGGTAAT GTTGTAGGAG
  241 CATGTTTAAT AAATTACTAT AGCAAATTAC TTTTTATTCC CAAGGTGTTT ATCTATAATA
  301 GTTAATATTT TAGTCGCTAC ATAAAATTTT ACCAAAGAGT ACTTGTATAC TAATTCTAAA
  361 TGCCTTCTGA CATAAAACGC CTAGGAAAAC AAACGCAAAC AAGGCATCGA CTTTTTCAAT
  421 AACCAACCAA AAAAATTTTA CATTAGTCTT TTTTTAATGC TGAGAAAGTC TTTGCTGATA
  481 TGCCTTCCAA CCAGCTTCTC TATATCTCTT GGCTTCGACA ACAGGATTAC GACCAGCTCC
  541 ATAGACTCCA CGACCAACAA TGATGATGTC GCTACCGCAG TTTACAATCA CTTCTTCAGG
  601 AGTACGATAT TGCTGTCCCA GCCCGTCTCC TTTAACATCC AAGCCGATAC CAGGGGACAT
  661 AGTTATGTAG TCGCTTTGAA GGTTAGGAAA TCGACGACCA GCTATAAAGC CAAAGCAAAA
  721 ATCGGTATGC TTCTCAAACC ATTCTAAGGT TTTCTCTGTG TAGGAACCAG TAGCCAAAGA
  781 GCCTTTGGAA GACATTTCAG CCAAAAGCAA GAGACCACGT CCCAAAGGTA AACCAACTTC
  841 TTTGAGGCCT TGTATAATAC CCTCGCCTGG CACTGTATGG CAATTTGTGA TATGAGCCCA
  901 AGAAGCAATT TTGTACACAC CAGATGCATA TTGTAGCTTT GACGTATTTT CCCAATGTCT
  961 GCGAATTTGG CGATCCTCAA GATAAGAAAC GATGCTTTTT TACCTAAGGC AACAAGTTTT
 1021 TCTACCATAT TCCTTGTTCG GAATCCTCGA CCAACCGTCG ATATGGTCGT GATTAGCCAG
1081 GAGTATGGTT CCGT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913. [link to RRC of NBRP]
original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162. [link to RRC of NBRP]