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pDUAL2-FFH81 (#RDB06201)

S. pombe 3 ter end FLAG2-His6-tagged vector with nmt1 (low strength) promoter

Clone info. S. pombe 3 ter end FLAG2-His6-tagged vector with nmt1 (low strength) promoter.
Comment Multicopy marker, ura4+; Integration marker, ura4+, leu1-. Gateway incompatible.
Vector backbone pDUAL2 (RDB06192) (Plasmid)
Selectable markers Amp^r
Reference of insert sequence AB364067.1 (DDBJ accession number)
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
 
Sequence RDB06201z.seq

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、　寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06201 pDUAL2-FFH81 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pDUAL2-FFH81 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06201).

Reference section:

Matsuyama, A., Yoshida, M., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913. [link to RRC of NBRP]
Matsuyama, A., Shirai, A., Yashiroda, Y., Kamata, A., Horinouchi, S., Yoshida, M., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14491_A6L7p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw
Sequence file: RDB14491_A6L7a.seq check
>06201_14491_A6L7_nmt1Fw_E04_13_ABI24.ab1
    1 TGCAATTTTA TTAATCAGGA AACGTAACTC TCGGCTACTG GATGGTTCAG TCACCCAACG
   61 ATTACTGGGG AGAGAAAACA GGGCAAAAGC AAAGCTTAAA GGAATCCGAT TGTCATTCGG
  121 CAATGTGCAG CGAAACTAAA AACCGGATAA TGGACCTGTT AATCGAAACA TTGAAGATGG
  181 AAGAGGAATC CTGGCATATC ATCAATTGAA TAAGTTGAAT TAATTATTTC AATCTCATTC
  241 TCACTTTCTG ACTTATAGTC GCTTTGTTAA AGCTAGCGTC GACAGGCCTG GATCCGGATA
  301 TCATGGATTA TAAGGATGAT GACGATAAAC CCATGGACTA CAAGGACGAC GATGACAAAC
  361 ATCACCACCA TCACCACTGA GATCTCGACT CTAGAGGATC CGGCGCGCCA CTTCTAAATA
  421 AGCGAATTTC TTATGATTTA TGATTTTTAT TATTAAATAA GTTATAAAAA AAATAAGTGT
  481 ATACAAATTT TAAAGTGACT CTTAGGTTTT AAAACGAAAA TTCTTATTCT TGAGTAACTC
  541 TTTCCTGTAG GTCAGGTTGC TTTCTCAGGT ATAGTATGAG GTCGCTCTTA TTGACCACAC
  601 CTCTACCGGC AGATCCGCTA GGGATAACAG GGTAATATAC TAGTGCCTCT ATTGATGCCT
  661 ATGGAACCCC TTTGACTGAC GAGACTGTGA AAGGCTTGTT TGGAAGCTGA CGGTGTTCTT
  721 TTGGGTGCCG TTGGTGGTCC TGAATGGACC AACCCCAATT GTCGTCCTGA GCAAGGTTTA
  781 TTGAAGCTTC GTAAGAGTAT GGGGTGTTTG GGGCCACCTT TCGACCTTGC AACTTTGCCA
  841 GCAAGTCTTT AGTCAAGTAC AGCCCTTTGA AGCCTGAATC GTGAGTGTCG ATTTTTTTGT
  901 GTTTGTACGA GACTTACTGG AGTGTACTTG GTGAGCGCAC TGAAGACACC GATCCGGTTA
  961 TGCTATGACA CTGCTTACAG TTTGGAGAGT ACTCGGTATG CTCGATAGCG ACGCGGGTCT
1021 ACTTCAATCA TATCGAGTTC TCATC
//
Primer: ADH-Forward
Sequence file: RDB14491_A6L7b.seq check
>06201_14491_A6L7_ADH-Forward_F04_16_ABI24.ab1
    1 TACTGCCTAT TGACACACCT CTACCGGCAG ATCCGCTAGG GATAACAGGG TAATATACTA
   61 GTGCCTCTAT TGATGCCTAT GGAACCCCTT TGACTGACGA GACTGTGAAG GCTTGTTTGG
  121 AAGCTGACGG TGTTCTTTTG GGTGCCGTTG GTGGTCCTGA ATGGACCAAC CCCAATTGTC
  181 GTCCTGAGCA AGGTTTATTG AAGCTTCGTA AGAGTATGGG TGTTTGGGCC AACCTTCGAC
  241 CTTGCAACTT TGCCAGCAAG TCTTTAGTCA AGTACAGCCC TTTGAAGCCT GAAATCGTTG
  301 AAGGTGTCGA TTTTTGTGTT GTACGAGAAC TTACTGGAGG TTGTTACTTT GGTGAGCGCA
  361 CTGAGGACAA CGGATCGGGT TATGCTATGG ACACTTGGCC TTACAGTTTG GAAGAAGTTT
  421 CTCGTATTGC TCGTTTGGCG GCCGCGGGCC CAACCTTCCA ATTCATTAAA TCGAGTCCTC
  481 CATCAGATGA TTTACAAGGT TTTGCATAGA ATCCCTAAGT AAGAGAAGTT GTTAGTGATT
  541 TCTTCAAGTT TACTAAAATT TACATACAAA TGGATGATCT CTTCTCCATT GTTTCCTTTC
  601 TTCTTGCAAA CGCGTTTTAC AAAGAGATGA CATGCTATTT TCTGATTATT TTTTTCTATA
  661 GTTTTCTATT TTGTACGTCC TTGGGAGTCC GAAATGTAAA ATCGGGGGTA TGGTTAGTAG
  721 CGGCAGCGTT CATTATGGTA TATAAAAGAT AAAAAGTAGG GGCAAGCGAG ACAGAATGGG
  781 ATACAAAGGG CATCGTCTAT AATTATAGCT AAAAATTGTA TTTTAAATTT GTATTTTTTT
  841 GTAATTTTAT TTCTCATTGT TTTACTTTAA AATGAAATGC GAATTAGAGA AAACTTTCAA
  901 CGAAATGTCA AAATAAGCTC AGCAAAAATA TAACAATTTT TAGGAAAGCG ATCAGCCAAC
  961 TATCTCGTTG TAGATTCCTT TTCCTCATTA ATCAACAATG TACTGGCGAT GATTTTTCTC
1021 TAGATGCCTA ATAAATTGAA CTAATGCATA TATACCACAG GTTCTGTA
//
Primer: f1ori_R3
Sequence file: RDB14491_A6L7c.seq check
>06201_14491_A6L7_f1ori_R3_G04_19_ABI24.ab1
    1 GGATGGGGCT TGCGTATGCG GTGTGAATAC CGCACAGATG CGTAAGGAGA AAATACCGCA
   61 TCAGGCGCCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT
  121 CTTCGCTATT ACGCCAGCTG GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA
  181 CGCCAGGGTT TTCCCAGTCA CGACGTTGTA AAACGACGGC CAGTGAATTC GAGCTCGGGA
  241 GCGCTACCGT GAATGGGCTC AACCAAACAA TGAACCTTTT CTTCTGATTT TCCTACCACA
  301 CCGGAAAGGG AGGCAGAAGG CAAAAGGCCC AAGCTACCAG GAATGACAGA AGCCTCATCT
  361 GAAATAATGT CACCAAACAA GTTGTCAGTC AAAACAACAC CGTTAAGTGT ACGAGGGCTC
  421 TTGACCAAAA GCATGGCTGC GGAGTCAATG AGCTGGTTTT TTAAGGTAAG GTGAGGATAT
  481 TCCTCCTTAA AAATCTTAGC TACAGTCTTG CGCCAAAGAC GAGAAGTTGC CAAAACATTA
  541 GCTTTGTCGA GTAATGTGAC GGGAGCAGGA GGGTTGGAAG TTTCAGCTAA GCGGCCGCGG
  601 GCCCGAGTCT AACTCCTTAA CCACTCGGAC ATAGTGACTT ATCTGACACT CATTGTAAAT
  661 TAATATATAT ATAGGCATTT TGTTTAGTTA AAGGTACTTA AGTAATTAGT ATAAACGAAC
  721 CAATTTTATA ATCAGGAAGT TAAGTGAATG GTAGCACATG TCGTAAAAAT TGTGAATTTT
  781 TATTGAATAA TATTTTAAAT ACAAGCCTTT CTAGACTAGG TATACTCATA AACATATGAG
  841 CAAAAGATAG AGGAGATTAC ATTGCATCTT CTACAAATTT ATTTTATTGC CTTTACTGAA
  901 AAATTAAATA ATGAGTACTA AAATGATAAA AAGCGCTCAG TACAAGAAAG ATGGCCAAAA
  961 TATGCATCCT CATGATTAAA GTGCCATATA GCCATATGGA AGTATAGGTA CTAAACAGCA
1021 GTAGCGAAAT CATGATTATC GCAAGACATG GAAACTGACT TAAGATGTTA
//
Primer: Reverse2
Sequence file: RDB14491_A6L7d.seq check
>06201_14491_A6L7_Reverse2_H04_22_ABI24.ab1
    1 CGGGAATGAT TACGCCAGCT TGTGATATTG ACGAAACTTT TTGACATCTA ATTTATTCTG
   61 TTCCAACACC AATGTTTATA ACCAAGTTTT ATCTTGTTTG TCTACATGGT ATTTTACATT
  121 CATCTACATA CATCTTTCAT TGGCTTTGTA CATAGTTATC ATTACAAGTC TAAAAAAATT
  181 CACTCTTTTC TTATTCAATG TCAATCCAAG AGAAAAGATT GTGGTAATGT TGTAGGAGCA
  241 TGTTTAATAA ATTACTATAG CAAATTACTT TTTATTCCCA AGGTGTTTAT CTATAATAGT
  301 TAATATTTTA GTCGCTACAT AAAATTTTAC CAAAGAGTAC TTGTATACTA ATTCTAAATG
  361 CCTTCTGACA TAAAACGCCT AGGAAAACAA ACGCAAACAA GGCATCGACT TTTTCAATAA
  421 CCAACCAAAA AAATTTTACA TTAGTCTTTT TTTAATGCTG AGAAAGTCTT TGCTGATATG
  481 CCTTCCAACC AGCTTCTCTA TATCTCTTGG CTTCGACAAC AGGATTACGA CCAGCTCCAT
  541 AGACTCCACG ACCAACAATG ATGATGTCGC TACCGCAGTT TACAATCACT TCTTCAGGAG
  601 TACGATATTG CTGTCCCAGC CCGTCTCCTT TAACATCCAA GCCGATACCA GGGGACATAG
  661 TTATGTAGTC GCTTTGAAGG TTAGGAAATC GACGACCAGC TATAAAGCCA AAGCAAAAAT
  721 CGGTATGCTT CTCAAACCAT TCTAAAGTTT TCTCTGTGTA GGAACCAGTA GCCAAAGAGC
  781 CTTTGGGAAG ACATTTCAGC CAAAAGCAAG AGACCACGTC CCAAAGTAAA CCAACTTCTT
  841 TGAGCCTGTA TAATACCCTC CGCTGCACTG TATGGCAATT TGTGATATGA GCCAGAGCAT
  901 TTTGTACACA CCAGATGCAT ATGGTAGCTG GACGTATTTC ATGTCTGCGA CTTGCGATCC
  961 TCAAGGATAG AAACGATGCT TTTAACTAGC ACAGTTTTCT ACGATTCTGG TCGAATCTGA
1021 CACGTCAAGT GCTGTACGGA CAATGGTCCA ATCTTAAGTC A
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913. [link to RRC of NBRP]
original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162. [link to RRC of NBRP]