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pET_HisFLAG-hsJDP2 (#RDB06215)

Expression vector, bacterial, of human JDP2

Alternative name clone 1
Clone info. Expression vector, bacterial, of human JDP2. His and FLAG tagged.
Comment Original clone was obtained from SETBANK.
Vector backbone pET15b (Plasmid)
Size of vector backbone 5.7 kb
Selectable markers Amp^r
Gene/insert name Human JDP2 cDNA
Depositor|Developer Yokoyama, Kazunari |
 

Distribution information

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Terms and conditions for distribution 1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
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MTAに書く使用条件 1. 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。　2. 謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06215 pET_HisFLAG-hsJDP2 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pET_HisFLAG-hsJDP2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06215).

Reference section:

Murata T., Shinozuka Y., Obata Y., Yokoyama K.K., Phosphorylation of two eukaryotic transcription factors, Jun dimerization protein 2 and activation transcription factor 2, in Escherichia coli by Jun N-terminal kinase 1. Anal. Biochem., 376, 115-121 (2008). PMID 18307971. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB06215_A6Jbp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pBR322_BamHI_R2
Sequence file: RDB06215_A6Jba.seq check
>06215_06215_A6Jb_3_lacIpro_F_B07_02_ABI08.ab1
    1 GCTTCATGAT GCGCGGAGAG AGTCATTCAG GTGGTGAATG TGAAACCAGT AACGTTATAC
   61 GATGTCGCAG AGTATGCCGG TGTCTCTTAT CAGACCGTTT CCCGCGTGGT GAACCAGGCC
  121 AGCCACGTTT CTGCGAAAAC GCGGGAAAAA GTGGAAGCGG CGATGGCGGA GCTGAATTAC
  181 ATTCCCAACC GCGTGGCACA ACAACTGGCG GGCAAACAGT CGTTGCTGAT TGGCGTTGCC
  241 ACCTCCAGTC TGGCCCTGCA CGCGCCGTCG CAAATTGTCG CGGCGATTAA ATCTCGCGCC
  301 GATCAACTGG GTGCCAGCGT GGTGGTGTCG ATGGTAGAAC GAAGCGGCGT CGAAGCCTGT
  361 AAAGCGGCGG TGCACAATCT TCTCGCGCAA CGCGTCAGTG GGCTGATCAT TAACTATCCG
  421 CTGGATGACC AGGATGCCAT TGCTGTGGAA GCTGCCTGCA CTAATGTTCC GGCGTTATTT
  481 CTTGATGTCT CTGACCAGAC ACCCATCAAC AGTATTATTT TCTCCCATGA AGACGGTACG
  541 CGACTGGGCG TGGAGCATCT GGTCGCATTG GGTCACCAGC AAATCGCGCT GTTAGCGGGC
  601 CCATTAAGTT CTGTCTCGGC GCGTCTGCGT CTGGCTGGCT GGCATAAATA TCTCACTCGC
  661 AATCAAATTC AGCCGATAGC GGAACGGGAA GGCGACTGGA GTGCCATGTC CGGTTTTCAA
  721 CAAACCATGC AAATGCTGAA TGAGGGCATC GTTCCCACTG CGATGCTGGT TGCCAACGAT
  781 CAGATGGCGC TGGGCGCAAT GCGCGCCATT ACCGAGTCCG GGCTGCGCGT TGGTGCGGAT
  841 ATCTCGGTAG TGGGATACGA CGATACCGAA GACAGCTCAT GTTATATCCC GCCGTTAACC
  901 ACCATCAAAC AGGATTTTCG CCTGCTGGGG CAACCAGCGT GGACCGCTTT GCTGCAACTC
  961 TCTCAGGCCC AGGCGGGTGA AAGGGCAATC AGCTGTTGCC CGGTCTCACT GGTGAAAGAA
 1021 AAACCACCCT GCCGCCATAC GCAAACCGCT CTTCCCCGCG CGTTGGTCGG ATTCATTAAA
1081 TGCAGCTGGC ACGACAGGTT CCGGACTGGA AAGGCGGGCC GAGTTGAAGC CGCCCAAAA
//
Primer: lacIpro_F
Sequence file: RDB06215_A6Jbb.seq check
>06215_06215_A6Jb_3_pBR322BamHI_R2_C02_03_ABI08.ab1
    1 CGAGGCTGTT AAAGCGGTTG AGCACGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC
   61 GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT
  121 GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC
  181 AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT
  241 CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGGAAT TGTGAGCGGA TAACAATTCC
  301 CCTCTAGAAA TAATTTTGTT TAACTTTAAG AAGGAGATAT ACCATGGGCA GCAGCCATCA
  361 TCATCATCAT CACAGCAGCG GCCTGGTGCC GCGCGGCAGC CATATGGACT ACAAGGACGA
  421 TGACGATAAG GCGGCCATGA TGCCTGGGCA GATCCCGGAC CCTTCGGTGA CCACAGGCTC
  481 CCTGCCAGGG CTTGGCCCCC TGACCGGGCT CCCCAGCTCG GCCCTGACTG TGGAGGAGCT
  541 GAAATACGCT GACATCCGCA ACCTCGGGGC CATGATTGCA CCCTTGCACT TCCTGGAGGT
  601 GAAACTGGGC AAGAGGCCCC AGCCCGTGAA AAGTGAGCTA GATGAGGAAG AGGAGCGAAG
  661 GAAAAGGCGC CGGGAGAAGA ACAAAGTCGC AGCAGCCCGA TGCCGGAACA AGAAGAAGGA
  721 GCGCACGGAG TTTCTGCAGC GGGAATCCGA GCGGCTGGAA CTCATGAACG CAGAGCTGAA
  781 GACCCAGATT GAGGAGCTGA AGCAGGAGCG GCAGCAGCTC ATCCTGATGC TGAACCGACA
  841 CCGCCCCACC TGCATCGTCC GGACCGACAG TGTCAAGACC CCCGAGTCAG AAGGCAACCC
  901 ACTGCTCGAG CAGCTCGAGA AGAAGTGAGG GGATCCGGCT GCTAACAAGC CCGAAAGGAA
  961 GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA
 1021 CGGGTCTTGA GGGGTTTTTT TGCTGAAGAA GACTATATCG GGATATCCCG CAGGAGCCGG
 1081 CAGGTACCGG CATACCAAGC TATGGCCATA CAGCCATTCC AGGGTGAACG GATGTCCCGG
1141 AAAG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Murata T., Phosphorylation of two eukaryotic transcription factors, Jun dimerization protein 2 and activation transcription factor 2, in Escherichia coli by Jun N-terminal kinase 1. Anal. Biochem., 376, 115-121 (2008). PMID 18307971. [link to RRC of NBRP]