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pET_His-hsJDP2 (#RDB06216)

Expression vector, bacterial, of human JDP2

Alternative name clone 5
Clone info. Expression vector, bacterial, of human JDP2. His tagged.
Comment Original clone was obtained from SETBANK.
Vector backbone pET15b (Plasmid)
Size of vector backbone 5.7 kb
Selectable markers Amp^r
Gene/insert name Human JDP2 cDNA
Depositor|Developer Yokoyama, Kazunari |
 

Distribution information

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Terms and conditions for distribution 1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06216 pET_His-hsJDP2 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pET_His-hsJDP2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06216).

Reference section:

Murata T., Shinozuka Y., Obata Y., Yokoyama K.K., Phosphorylation of two eukaryotic transcription factors, Jun dimerization protein 2 and activation transcription factor 2, in Escherichia coli by Jun N-terminal kinase 1. Anal. Biochem., 376, 115-121 (2008). PMID 18307971. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB06216_A6Jbp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pBR322_BamHI_R2
Sequence file: RDB06216_A6Jba.seq check
>06216_06216_A6Jb_1_pBR322BamHI_R2_C07_03_ABI08.ab1
    1 CCTCGTGGAG GCCGTTGAGC ACGCCGCCGC AGGAATGGTG CATGCAAGGA GATGGCGCCC
   61 AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC
  121 CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC
  181 GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG
  241 ATCCCGCGAA ATTAATACGA CTCACTATAG GGGAATTGTG AGCGGATAAC AATTCCCCTC
  301 TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGGCAGCAG CCATCATCAT
  361 CATCATCACA GCAGCGGCCT GGTGCCGCGC GGCAGCCATA TGGCGGCCAT GATGCCTGGG
  421 CAGATCCCGG ACCCTTCGGT GACCACAGGC TCCCTGCCAG GGCTTGGCCC CCTGACCGGG
  481 CTCCCCAGCT CGGCCCTGAC TGTGGAGGAG CTGAAATACG CTGACATCCG CAACCTCGGG
  541 GCCATGATTG CACCCTTGCA CTTCCTGGAG GTGAAACTGG GCAAGAGGCC CCAGCCCGTG
  601 AAAAGTGAGC TAGATGAGGA AGAGGAGCGA AGGAAAAGGC GCCGGGAGAA GAACAAAGTC
  661 GCAGCAGCCC GATGCCGGAA CAAGAAGAAG GAGCGCACGG AGTTTCTGCA GCGGGAATCC
  721 GAGCGGCTGG AACTCATGAA CGCAGAGCTG AAGACCCAGA TTGAGGAGCT GAAGCAGGAG
  781 CGGCAGCAGC TCATCCTGAT GCTGAACCGA CACCGCCCCA CCTGCATCGT CCGGACCGAC
  841 AGTGTCAAGA CCCCCGAGTC AGAAGGCAAC CCACTGCTCG AGCAGCTCGA GAAGAAGTGA
  901 GGGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG
  961 CAATAACTAG CATAACCCCT TTGGGGCCTC TAAACGGGTC TTGAGGGGGT TTTTTGCTGA
 1021 AGGAGGACTA TATCCCGGAT ATCCCGGCAG AGGCCGCAGG TACGGGCATA GCTAAGGCTA
 1081 ATGGCTAACA GCCATTCAGG TGACGGTGCT GAGCATGACG AATGGACGCC ATTGTTTAGG
1141 AAATTTTCA
//
Primer: lacIpro_F
Sequence file: RDB06216_A6Jbb.seq check
>06216_06216_A6Jb_1_lacIpro_F_H02_08_ABI08.ab1
    1 CCCTGATTGG GCTCAGGGAT GCGCGGAGAG AGTCAATTCA GGGTGGTGAA TGTGAAACCA
   61 GTAACGTTAT ACGATGTCGC AGAGTATGCC GGTGTCTCTT ATCAGACCGT TTCCCGCGTG
  121 GTGAACCAGG CCAGCCACGT TTCTGCGAAA ACGCGGGAAA AAGTGGAAGC GGCGATGGCG
  181 GAGCTGAATT ACATTCCCAA CCGCGTGGCA CAACAACTGG CGGGCAAACA GTCGTTGCTG
  241 ATTGGCGTTG CCACCTCCAG TCTGGCCCTG CACGCGCCGT CGCAAATTGT CGCGGCGATT
  301 AAATCTCGCG CCGATCAACT GGGTGCCAGC GTGGTGGTGT CGATGGTAGA ACGAAGCGGC
  361 GTCGAAGCCT GTAAAGCGGC GGTGCACAAT CTTCTCGCGC AACGCGTCAG TGGGCTGATC
  421 ATTAACTATC CGCTGGATGA CCAGGATGCC ATTGCTGTGG AAGCTGCCTG CACTAATGTT
  481 CCGGCGTTAT TTCTTGATGT CTCTGACCAG ACACCCATCA ACAGTATTAT TTTCTCCCAT
  541 GAAGACGGTA CGCGACTGGG CGTGGAGCAT CTGGTCGCAT TGGGTCACCA GCAAAGCGCG
  601 CTGATAGCGG GCCCATAAAG TTCTGTCTCG GCGCGTCTGC GTCTGGCTGG CTGGCATAAA
  661 TATCTCACTC GCAATCAAAA TTCAGCCGAT AGCGGAACGG GAAGGCGACT GGAGTGCCAT
  721 GTCCGGTTTT CAACAAACCA TGCAAATGCT GAATGAGGGC ATCGTTCCCA CTGCGATGCT
  781 GGTTGCCAAC GATCAAAATG GCGCTGGGCG CAATGCGCGC CATTACCGAG TCCGGGGCTG
  841 CGCGTTGGTG CGGGATATCT CGGTAGTGGG ATACGACGAT ACCCGAAGAC AGCTCATGTT
  901 ATATCCCGCC GTTAAACCAC CATCAAAACA GGATTTTCGG CCTGCTGGGG CAAACCAAGC
  961 GTTGGACCGC TTTGCTGCAA CTCTCTCAGG GCCCAGGCCG GTTGAAGGCC AATCCAGCCT
 1021 GTATGCCTCC GTCTCACTGA TTGGAAAAGA AAAAACTCAA CCCCATGGCT GCCCAATACG
 1081 CAAACCGTCT CCTCCCCGCG CGTGGCCGGA ATTCCAATAA TGCCAGCCTT GGCAACCGAA
1141 CAAGATTTC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Murata T., Phosphorylation of two eukaryotic transcription factors, Jun dimerization protein 2 and activation transcription factor 2, in Escherichia coli by Jun N-terminal kinase 1. Anal. Biochem., 376, 115-121 (2008). PMID 18307971. [link to RRC of NBRP]