Expression vector of human JUN.
Alternative name | SET-0010_37 |
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Clone info. | Expression vector of human JUN. Expression was confirmed by Western blotting with anti-FLAG antibody. |
Comment | PCR cloning, forward primer: atgactgcaaagatggaaacg; reverse primer: tcaaaatgtttgcaactgctg. |
Vector backbone | pCMV_S-FLAG (RDB05956) (Plasmid) |
Size of vector backbone | 5.5 kb |
Selectable markers | Amp^r (E. coli), Neo^r (mammalian cell) |
Gene/insert name | Human c-jun cDNA |
Depositor|Developer | DNA Bank, | |
Remarks, protocol and/or map (pdf) | RDB06254.pdf |
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Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. |
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Terms and conditions for distribution | The RECIPIENT agrees to describe RIKEN BRC as the source of the MATERIAL in any publication. |
必要書類 | 提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
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MTAに書く使用条件 | 発表に際し、理研BRCから入手したことを明記すること。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB06254 | pCMFlag_hsJUN | DNA solution |
JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge |
Materials & Methods section:
The pCMFlag_hsJUN was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06254). |
Reference section:
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB08129_A1Fgp1-1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
Primer: CMV_Forward (Pr0016) Region: CMV pro,FLAG,insert 5' Sequence file: RDB08129_A1Fga.seq |
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>06254_08129_B1AjF_CMV-Forward_C10_07_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNTGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA 61 GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA 121 CTCACTATAG GGAGACCCAA GCTGAATTCA CCATGGACTA CAAGGACGAT GACGATAAGG 181 CGGCCATGAC TGCAAAGATG GAAACGACCT TCTATGACGA TGCCCTCAAC GCCTCGTTCC 241 TCCCGTCCGA GAGCGGACCT TATGGCTACA GTAACCCCAA GATCCTGAAA CAGAGCATGA 301 CCCTGAACCT GGCCGACCCA GTGGGGAGCC TGAAGCCGCA CCTCCGCGCC AAGAACTCGG 361 ACCTCCTCAC CTCGCCCGAC GTGGGGCTGC TCAAGCTGGC GTCGCCCGAG CTGGAGCGCC 421 TGATAATCCA GTCCAGCAAC GGGCACATCA CCACCACGCC GACCCCCACC CAGTTCCTGT 481 GCCCCAAGAA CGTGACAGAT GAGCAGGAGG GCTTCGCCGA GGGCTTCGTG CGCGCCCTGG 541 CCGAACTGCA CAGCCAGAAC ACGCTGCCNA GCGTCNCGTC NGCGGCGCAG CCGGTCAACG 601 GGGCANGCAT GGTGGCTCCN GCGGTAGCCT CGGTGGNANG GGGCANCGGC AGCGGCNGCT 661 TCNGCGCCNG CCNGNACANC GAGNCNNNNG TCTACNNNAA NNNCNNCNNC NTNNNNCNNN 721 NCNNNCTGAN NNNNNNNNN // |
Primer: bGH_rev3 (Pr0885) Region: bGHpA,insert 3' Sequence file: RDB08129_A1Fgb.seq |
>06254_08129_B1AjF_bGH_rev3_C11_08_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNGCAA ACNACAGATG GCTGGCAACT AGAAGGCACA 61 GTCGAGGCTG ATCAGCGAGC TCTAGCATTT AGGTGACACT ATAGAATAGG GCCCTCTAGA 121 TGCATGCTCG AGCGGCCTCA AAATGTTTGC AACTGCTGCG TTAGCATGAG TTGGCACCCA 181 CTGTTAACGT GGTTCATGAC TTTCTGTTTA AGCTGTGCCA CCTGTTCCCT GAGCATGTTG 241 GCCGTGGACG CCAGCTCCGA GTTCTGAGCT TTCAAGGTTT TCACTTTTTC CTCCAGCCGG 301 GCGATTCTCT CCAGCTTCCT TTTTCGGCAC TTGGAGGCAG CGATGCGGTT CCTCATGCGC 361 TTCCTCTCCG CCTTGATCCG CTCCTGGGAC TCCATGTCGA TGGGGGACAG GGGCGGTGTC 421 TCGCCGGGCA TCTCGGGCAC TGTCTGAGGC TCCTCCTTCA GGGCCTGCAG CCGCGGGTGC 481 TGCACGGGCA TCTGCTGGGG CAGGTGGTGC GGCGGCTGCT GCTGCTGCTG GGGTTGCGCG 541 GGAAAGGCCA GGCCGGCCGC GCCGTANGAN GGCGCCCCGC CGCCGCTGCT CNGCGCGNCT 601 GGGTTGAAGT TGCTGANGTT TGCGTANANC GGCGGCTCGC TGTGCNNGNT GGCGCTNNNN 661 CNCCGCTGCC GCNGNCCCCN GNCNNCNNNN NNNNNCNNNN CNNNCNNGCN TGNCCCNNTN 721 ANCNGNTGNN CNNNNNANNT NNNNNNNGNN NNNNNNNN // |
Primer: SV40pro_ori_F (Pr0731) Region: SV40 pro_ori,NeoR Sequence file: RDB08129_A1Fgc.seq |
>06254_08129_B1AjF_SV40pro_ori_F_C12_09_ABI24.ab1 1 NNNNNNNNNN NNNNGNCGCC TCNGCCTCTG ANCTATTCCN GAAGTAGTGA GGAGGCTTTT 61 TTGGAGGCCT AGGCTTTTGC AAAAAGCTCC CGGGAGCTTG GATATCCATT TTCGGATCTG 121 ATCAAGAGAC AGGATGAGGA TCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT 181 CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT 241 GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA 301 CCGACCTGTC CGGTGCCCTG AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG 361 CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT 421 GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG 481 AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT 541 GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG 601 GTCTTGTCGA TCANGANGAN CTGNNCNAAN ANCATCNNGG NCTCGCGCCN GCCNAACTGN 661 TCGCCNGGCT NNNNNNNCGC ATGNCNNANN GCNANNNNNN CGNCNNGNNC NNNNNNNNNG 721 NNNGNNNNNN NNNN // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
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