Please review the QC test results indicated by 
icon below as well as clone information before placing your order.
提供案内 (日本国内) 
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Please review the QC test results indicated by icon below as well as clone information before placing your order.
icon below as well as clone information before placing your order.
pCMFlag_hsATF2 (#RDB06257)
Expression vector of human ATF2.
| Alternative name | SET-0021_11 |
|---|---|
| Clone info. | Expression vector of human ATF2. Expression was confirmed by Western blotting with anti-FLAG antibody. |
| Comment | PCR cloning, forward primer: atgaaattcaagttacatgtg; reverse primer: tcaacttcctgagggctgtga. cDNA fragment 297 to 1814nt of NM_001880.4 (cds 297..1814nt) was cloned. Known difference : T to G substitution at 1298nt of NM_001880.4 (Phe to Val). |
| Vector backbone | pCMV_S-FLAG (RDB05956) (Plasmid) |
| Size of vector backbone | 5.5 kb |
| Selectable markers | Amp^r (E. coli), Neo^r (mammalian cell) |
| Growth conditions | 37C, LB+Amp |
| Gene/insert name | Human CREB2; ATF2 cDNA |
| Depositor|Developer | DNA Bank, | |
| Remarks, protocol and/or map (pdf) | RDB06257.pdf |
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Distribution information
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Ordering forms | Order form [Credit Card Payment  ] [Bank Transfer Payment] [Example of order form ]MTA, for use for not-for-profit academic purpose [Word ] [Example of MTA ]MTA, for use for for-profit purpose [Word ]Please visit Information of Request for Distribution.[link] |
|---|---|
| Terms and conditions for distribution | The RECIPIENT agrees to describe RIKEN BRC as the source of the MATERIAL in any publication. Additional terms and conditions: Regrding pRc/CMV: a) The modified pRc/CMV, and their descendants (collectively, LIFE MATERIALS), should be used for pre-clinical/non-human, research use only. b) LIFE MATERIALS shall not be used for any commercial purposes. c) LIFE MATERIALS shall not be transferred beyond the specified recipient. d) Recipient must contact Thermo Fisher Scientific Inc. at outlicense@thermofisher.com for any desired use of LIFE MATERIALS beyond the scope of a) to c) above. |
[open/close]| 必要書類 | 提供依頼書 [依頼書の記入例 ]提供同意書 (MTA, 非営利学術目的用)[Word ] [MTAの記入例 ]提供同意書 (MTA, 営利目的用)[Word ]手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
|---|---|
| MTAに書く使用条件 | 発表に際し、理研BRCから入手したことを明記すること。 付加的使用条件： pRc/CMVについて: a) The modified pRc/CMV, and their descendants (collectively, LIFE MATERIALS), should be used for pre-clinical/non-human, research use only. b) LIFE MATERIALS shall not be used for any commercial purposes. c) LIFE MATERIALS shall not be transferred beyond the specified recipient. d) Recipient must contact Thermo Fisher Scientific Inc. at outlicense@thermofisher.com for any desired use of LIFE MATERIALS beyond the scope of a) to c) above. |
| Catalog # | Resource name | Shipping form | Fee |
|---|---|---|---|
| RDB06257 | pCMFlag_hsATF2 | DNA solution |
JPY 9,460 (not-for-profit academic purpose) JPY 18,920 (for-profit-research purpose) plus cost of shipping containers, dry ice (if required) and shipping charge |
icon below as well as clone information before placing your order.How to cite this biological resource
Materials & Methods section:
| The pCMFlag_hsATF2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06257). |
Reference section:
Further references such as user reports and related articles (go to bottom)
QC test results
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
| Test sheet | RDB17941_B0B4p1-1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
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Primer: CMV_Forward (Pr0016) Region: FLAG,insert5' Sequence file: RDB17941_B0B4a.seq
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>06257_17941_B0B4_CMV_Forward_A01_01_ABI24.ab1 1 NNNNNNNNNN NNNNNNGNNC GNTAGGNNNG TACGGTGGGA GGNCTATATA AGCAGAGCTC 61 TCTGGCTAAC TAGAGAACCC ACTGCTTACT GGCTTATCGA AATTAATACG ACTCACTATA 121 GGGAGACCCA AGCTGAATTC ACCATGGACT ACAAGGACGA TGACGATAAG GCGGCCATGA 181 AATTCAAGTT ACATGTGAAT TCTGCCAGGC AATACAAGGA CCTGTGGAAT ATGAGTGATG 241 ACAAACCCTT TCTATGTACT GCGCCTGGAT GTGGCCAGCG TTTTACCAAC GAGGATCATT 301 TGGCTGTCCA TAAACATAAA CATGAGATGA CACTGAAATT TGGTCCAGCA CGTAATGACA 361 GTGTCATTGT GGCTGATCAG ACCCCAACAC CAACAAGATT CTTGAAAAAC TGTGAAGAAG 421 TGGGTTTGTT TAATGAGTTG GCGAGTCCAT TTGAGAATGA ATTCAAGAAA GCTTCAGAAG 481 ANGACATTAA AAAAATGCCT CTAGATTTAT CCCCTCTTGC AACACCTATC ATAAGAAGCA 541 AAATTGAGGA GCCTTCTGNT GTAGAAACNA CTCACCAGGA TAGTCCTTTA CCTCACCCAG 601 AGTCTACTAC CNGTGATGAG AANGAAGTAC CATTGGCACA AACTGCACAG CCCACATCAG 661 CTATTGNTCG TNCAGCATCA TTNCNGGTTC CNNATGNGCT GNTTANNNNT TCTGANTCNN 721 NNGNNNNNNN TNNGCNNNNA GTACNNTNNN NNANCNTCNN GNACNGNNNN CNCCNNNNCN 781 NNNTNNTNNN NNNNNNN // |
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Primer: BGH_rev2 (Pr0606) Region: bGHp(A),insert3' Sequence file: RDB17941_B0B4b.seq
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>06257_17941_B0B4_BGH_rev2_B01_04_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNAACNG NNGGCTGGCA ACTAGAAGGC ACAGTCGAGG 61 CTGATCAGCG AGCTCTAGCA TTTAGGTGAC ACTATAGAAT AGGGCCCTCT AGATGCATGC 121 TCGAGCGGCC TCAACTTCCT GAGGGCTGTG ACTGGGAGGA AGGAGCCATA ACGATCTGTG 181 AAAGAGCAGG CTCTGTACTC TGGTCCGCCA TCTGGGTGAG GACTGAAGTG GCTACAGCTT 241 CTGCCTTGGA GGTTGAACTG ACTCCATTGG ATGTGCTGAC CGAACTATGC TGTATAGCTT 301 CTGTATGTGG ACTACTCGGC ACTGAAATGT CTTCTGAACT ATCATCTTTA TCAGCAGTAT 361 GATAGCCAGA TTTCTTCTGC ATGGCGGTTA CAGGGCAATC TTTATGAGCC AGAAGAAGCT 421 GTTTCAGCTG TGCCACTTCA TTTCTCAGCA GGGTGACTTC ACTCTGCAGC TGACCATTTA 481 ATGAACTCAA GTCTTCAGCT TTCTTCTCTA AAGACTGAAC CCAGACTTTC CTTTTTTGTC 541 GGCATCTTGA AGCTGCTGCT CTATTTCGCT CTAAAACCTT TCTCCTTTTT TCATCAGGAT 601 CTTCGTTAGC TGCTCTTCTC CGACGACCAC TTGTACTTTG GGTCTGTGGA GTTGTGTGAG 661 CTGGAGAAGC CGGAGTTTCT GTAGTGGATG TGGCTGGCTG TTGTAATGAC TGCGGTCGAG 721 ATTCCTCTGA CTGAGTCCTA ACCAATCCGC TACCATGACC TTTGACAGTA TCACCATTGG 781 TAACTGGAGG ANGTTGCTGG GTCAAAGCAN CNTTTAATCT CATTTTTGCT TCTGANTGTN 841 CTGNTTGGGG ANANGAANGA NCTGGGNNTC CTGNNACNCT NNNNCCATGG NGNNTGGNCN 901 AANGANNGGG NNTGCNGCTG GNNCATGCNN NTTANAACNN GNNNNNGNNG CNNNNNNNN // |
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Primer: SV40pro_ori_F (Pr0731) Region: SV40 pro_ori,NeoR Sequence file: RDB17941_B0B4c.seq
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>06257_17941_B0B4_SV40pro_ori_F_C01_07_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNCTC TGAGCTATTC CNGAAGTAGT GAGGAGGCTT 61 TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGGATATCCA TTTTCGGATC 121 TGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG 181 TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG 241 CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA 301 GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT 361 GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA 421 CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC 481 CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC 541 CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC 601 CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT 661 GTTCGCCAGG CTCAAGGCGC GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA 721 TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG 781 CCGGCTGGGT GTGGCGGANC GCTATCAGGA CATAGCGTTG GCTACNCGTG ATATTGCTGA 841 AGAGCTTGGC GGCGAANGGG GCTGANCGCT TCCNCGTGCT TTACGGTATC GCCGCNCCCG 901 ATTCGCAGCG CATCGCCNTC TATCGCCTTC TTGNNGANTT CTTCTGANCG GGNNNCNNGG 961 GGTTCGAAAT GANNNANNNN NCGNNGCCCN ANCNNNNNAT CNCNANNNTN NNANTNNNNN 1021 NNNNN // |
Please visit 
Sequencing and PCR primers for primer information.
References
Original, user report and related articles
Resource search
Link
Other resources in our bankExternal Database
Human CREB2; ATF2
Reference sequence
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