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pCMFlag_hsMAFB

Expression vector of human MAFB.

Catalog number RDB06264
Resource name pCMFlag_hsMAFB
Alternative name SET-0059_32
Clone info. Expression vector of human MAFB. Expression was confirmed by Western blotting with anti-FLAG antibody.
Comment PCR cloning, forward primer: gccgcggagctgagcatggggccagag; reverse primer: tcacagaaagaactcgggagaggaggggct.
Vector backbone pCMV_S-FLAG (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human KRML; MAFB cDNA
Depositor|Developer DNA Bank, |
Other clones in our bank
External Database
Human KRML; MAFB
          Reference sequence
            
           
          Remarks, protocol and/or map (pdf) RDB06264.pdf

          Distribution information

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          RDB06264 pCMFlag_hsMAFB DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pCMFlag_hsMAFB was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06264).

          Reference section:

          Further references such as user reports and related articles (go to bottom)

          References and tips

          Featured content

          Featured content pCMV_tag and pRSV_tag assay-ready clones (English text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB19601_B2Dkp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward (Pr0016)
          Region: CMV pro,FLAG,insert 5'
          Sequence file: RDB19601_B2Dka.seq check
          >06264_19601_B2Dk_CMV_Forward_F01_06_ABI08.ab1
              1 NNNNNNNNNN NNNNNTNNNN GCNNATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA
             61 AGCAGAGCTC TCTGGCTAAC TAGAGAACCC ACTGCTTACT GGCTTATCGA AATTAATACG
            121 ACTCACTATA GGGAGACCCA AGCTGAATTC ACCATGGACT ACAAGGACGA TGACGATAAG
            181 GCGGCCGCCG CGGAGCTGAG CATGGGGCCA GAGCTGCCCA CCAGCCCGCT GGCCATGGAG
            241 TATGTCAACG ACTTCGACCT GCTCAAGTTC GACGTGAAGA AGGAGCCACT GGGGCGCGCG
            301 GAGCGTCCGG GCAGGCCCTG CACACGCCTG CAGCCAGCCG GCTCGGTGTC CTCCACACCG
            361 CTCAGCACTC CGTGTAGCTC CGTGCCCTCG TCGCCCAGCT TCAGCCCGAC CGAACAGAAG
            421 ACACACCTCG AGGATCTGTA CTGGATGGCG AGCAACTACC AGCAGATGAA CCCCGAGGCG
            481 CTCAACCTGA CGCCCGAGGA CGCGGTGGAA GCGCTCATCG GCTCGCACCC AGTGCCACAG
            541 CCGCTGCAAA GCTTCGACAG CTTTCGCGGC GCTCACCACC ACCACCATCA CCACCACCCT
            601 CACCCGCACC ACGCGTACCC GGGCGCCGGC GTGGCCNACG ACGANCTGGG CCNGCACGCT
            661 CNCCCGCACC ATCNCCATCA TCNNNNAGCG TCNCCNCCGN NNTNNNGCNN NNCTANCCCN
          721 GNNNNNNNNN CNNNCNNNNN NNNNN
          //
          Primer: bGH_rev3 (Pr0885)
          Region: bGH pA,insert 3'
          Sequence file: RDB19601_B2Dkb.seq check
          >06264_19601_B2Dk_bGH_rev3_G01_07_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNGGG GNNGGGCNNA CAACAGATGG CTGGCAACTA GAAGGCACAG
             61 TCGAGGCTGA TCAGCGAGCT CTAGCATTTA GGTGACACTA TAGAATAGGG CCCTCTAGAT
            121 GCATGCTCGA GCGGCCTCAC AGAAAGAACT CGGGAGAGGA GGGGCTGTCG CTGGTGGAGC
            181 CCGCCTCCCT GAAGCCGGAG TTGGCGAGTT TCTCGCACTT GACCTTGTAG GCGTCTCTCT
            241 CGCGGGCCAG CCGGGACACC TCCTGCTTAA GCTGCTCCAC CTGCTGAATG AGCTGCGTCT
            301 TCTCATTCTC CAGGTGGTGC TTCTGCTGGA CGCGTTTATA CCTGCAAGAC TGGGCGTAGC
            361 CCCGGTTCTT CAGGGTCCGC CGCTTCTGCT TCAGGCGGAT CACCTCGTCC TTGGTGAAGC
            421 CCCGCAGGTG GCGGTTCAGC TCGCGCACGG ACATGGACAC GAGCTGGTCG TCGGAGAAGC
            481 GGTCCTCCAC GCTGCCGTTG CCGCCCGCCG CCGTCGCCGA GGCCGTCGCG TGCGGCCCGG
            541 GCCCGGGGTG GCTAGTGGGC AGCTGTTGCG CCGGGCTAGC GGCGCTGGAC GGCGGCGGCG
            601 ACGCTTGGTG ANGATGGTGA TGGTGCGGGT GAGCGTGCGG GCCCNGCTCG TCNTGGGCCA
          661 CGCCNGCGCC NNGGNACNCN NNGNGCGGGT GANGGTGGTG NNGATGGNNN NNNNNNN
          //
          Primer: SV40pro_ori_F (Pr0731)
          Region: SV40 pro_ori,NeoR
          Sequence file: RDB19601_B2Dkc.seq check
          >06264_19601_B2Dk_SV40pro_ori_F_H01_08_ABI08.ab1
              1 NNNNNNNNNN NNNGCCGCCT CNGCCTCTGA NCTATTCCNG AAGTAGTGAG GAGGCTTTTT
             61 TGGAGGCCTA GGCTTTTGCA AAAAGCTCCC GGGAGCTTGG ATATCCATTT TCGGATCTGA
            121 TCAAGAGACA GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC
            181 TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG
            241 CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC
            301 CGACCTGTCC GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC
            361 CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG
            421 GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA
            481 GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG
            541 CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG
            601 TCTTGTCGAT CAGGANGANC TGGACNAAGA GCATCANGGG CTCGCGCCAG CCGAACTGNT
            661 CGCCAGGCTC NNNGCGCGCA NGNCCNANNG CNANGNNCTC NNCNNGNNCC NNGGNNANGC
          721 CNGCNNNNCN ANNNNNCNNG GNNNNAANNN NNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          2023.05.01

          GNP_filter3_RDBDEP_html_230501.pl