Please review the QC test results indicated by icon below as well as clone information before placing your order.

pCMFlag_hsNFE2L2 (#RDB06265)

Expression vector of human NFE2L2.

Alternative name	SET-0075_47
Clone info.	Expression vector of human NFE2L2. Expression was confirmed by Western blotting with anti-FLAG antibody.
Comment	PCR cloning, forward primer: atgatggacttggagctgccg; reverse primer: ctagtttttcttaacatctggctt.
Vector backbone	pCMV_S-FLAG (RDB05956) (Plasmid)
Size of vector backbone	5.5 kb
Selectable markers	Amp^r (E. coli), Neo^r (mammalian cell)
Growth conditions	37C, LB+Amp
Gene/insert name	Human NRF2; NFE2L2 cDNA
Depositor|Developer	DNA Bank, |

 

Remarks, protocol and/or map (pdf)	RDB06265.pdf

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Terms and conditions for distribution

The RECIPIENT agrees to describe RIKEN BRC as the source of the MATERIAL in any publication. Additional terms and conditions: Regrding pRc/CMV: a) The modified pRc/CMV, and their descendants (collectively, LIFE MATERIALS), should be used for pre-clinical/non-human, research use only. b) LIFE MATERIALS shall not be used for any commercial purposes. c) LIFE MATERIALS shall not be transferred beyond the specified recipient. d) Recipient must contact Thermo Fisher Scientific Inc. at outlicense@thermofisher.com for any desired use of LIFE MATERIALS beyond the scope of a) to c) above.

提供案内 (日本国内) [open/close]

Catalog #	Resource name	Shipping form	Fee
RDB06265	pCMFlag_hsNFE2L2	DNA solution	JPY 9,460 (not-for-profit academic purpose) JPY 18,920 (for-profit-research purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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How to cite this biological resource

Materials & Methods section:

The pCMFlag_hsNFE2L2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06265).

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB06265_A7Gop1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward Sequence file: RDB06265_A7Goa.seq
>6265_6265_A7Go_CMV-Forward_A01_01_ABI08.ab1     1 CCTCAACTCC GCCCATTGAC GCAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA    61 GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA   121 CTCACTATAG GGAGACCCAA GCTGAATTCA CCATGGACTA CAAGGACGAT GACGATAAGG   181 CGGCCATGAT GGACTTGGAG CTGCCGCCGC CGGGACTCCC GTCCCAGCAG GACATGGATT   241 TGATTGACAT ACTTTGGAGG CAAGATATAG ATCTTGGAGT AAGTCGAGAA GTATTTGACT   301 TCAGTCAGCG ACGGAAAGAG TATGAGCTGG AAAAACAGAA AAAACTTGAA AAGGAAAGAC   361 AAGAACAACT CCAAAAGGAG CAAGAGAAAG CCTTTTTCGC TCAGTTACAA CTAGATGAAG   421 AGACAGGTGA ATTTCTCCCA ATTCAGCCAG CCCAGCACAT CCAGTCAGAA ACCAGTGGAT   481 CTGCCAACTA CTCCCAGGTT GCCCACATTC CCAAATCAGA TGCTTTGTAC TTTGATGACT   541 GCATGCAGCT TTTGGCGCAG ACATTCCCGT TTGTAGATGA CAATGAGGTT TCTTCGGCTA   601 CGTTTCAGTC ACTTGTTCCT GATATTCCCG GTCACATCGA GAGCCCAGTC TTCATTGCTA   661 CTAATCAGGC TCAGTCACCT GAAACTTCTG TTGCTCAGGT AGCCCCTGTT GATTTAGACG   721 GTATGCAACA GGACATTGAG CAAGTTTGGG AGGAGCTATT ATCCATTCCT GAGTTACAGT   781 GTCTTAATAT TGAAAATGAC AAGCTGGTTG AGACTACCAT GGTTCCAAGT CCAGAAGCCA   841 AACTGACAGA AGTTGACAAT TATCATTTTT TACTCATCTA TACCCTCAAT GGGAAAAAAG   901 AAGTAGGTAA CTGTAGTCCA CATTTTTCTT TAATGCTTTT TGAGGGATTC CCTTTCAGCA   961 GCATCCCTCT CCACAGAAGA CCCCAACCCA GTTGACAGTG AACTCATTAA AATTCAGATG  1021 CCACAGTCCA ACCACAGATT TTTGGTGAAT GAAGTTTTAA TTCCTGCTTT TCATAGCTTG 1081 AAGCCAAGTA TTCAGCAACA GCATTGACCC CGTTCAGCG //
Primer: BGH_rev2 Sequence file: RDB06265_A7Gob.seq
>06265_06265_A7Go_BGH_rev2_A01_01_ABI24.ab1     1 CTTACGGGTT CAGTAGGCAC GGGGGAGGGG CAACAACAGA TGGCTGGCAA CTAGAAGGCA    61 CAGTCGAGGC TGATCAGCGA GCTCTAGCAT TTAGGTGACA CTATAGAATA GGGCCCTCTA   121 GATGCATGCT CGAGCGGCCC TAGTTTTTCT TAACATCTGG CTTCTTACTT TTGGGAACAA   181 GGAAAACATT GCCATCTCTT GTTTGCTGCA GGGAGTATTC ACTAGGAGAA TAAGGTTTTC   241 CATCTTCATC ACGTAGCATG CTGAAAACTT CGAGATATAA GGTGCTGAGT TGTTTTTTCA   301 GTAGGTGAAG GCTTTTGTCA TTTTCTCCTT TTTCTTTGAG CAATTTTTCT TTTTCATCTT   361 TCAAATGATC TAAATCTTGC TCTAGTTCTA CTATATTTTC CAGTTTTCTT TTTCTGCAAT   421 TCTGAGCAGC CACTTTATTC TTACCCCTCC TACGTATATC CCGAATTAAT GCAAGTTGAG   481 CTTCATTGAA CTGCTCTTTG GACATCATTT CGTTGAAGTC AACAACAGGG AGGTTAATGA   541 TTTTTTCTAC AGGGAATGGG ATATGGAGAG CTTTTGCCCT AAGTTCATCT CTTGTGAGAT   601 GAGCCTCCAA GCGGCTTGAA TGTTTGTCTT TTGTGAATGG GGTTTTCCGA TGACCAGGAC   661 TTACAGGCAA TTCTTTCTCT GGTGTGTTCT CACATTGGGC ATCATGCACG TGAGTGCTCT   721 GCCCCTGAGA TGGTGACAAG GGTTGTACCA TATCCCCAGA AGAATGTACT GGTGTTTTAG   781 GACCATTCTG TTTGACACTT CCAGGGGCAC TATCTAGCTC TTCCACTTCA GAATCACTGA   841 GGCCAAGTAG TGTGTCTCCA TAGCTGGAAG ATTCCACTGA GTGTTCTGGT GATGCCACAC   901 TGGGACTTGT GTTTAGTGAA ATGCCGGAGT CAGAATCATT GAATTCTGCT GTGCTTTCAG   961 GGTGGTTTTG GTTGAAAGCT TTGCAAAGTG ATAGATCAGA AAACATCAAA TGGGCCCATT  1021 TAAGAAAGTT TCAGAGGAGT GAATGGCTTA AAAGTAGCAC TGAGGGCCAT GCTTGTTGCT  1081 GATACTTAGC TCAGCTATGA AGCCAGAATA AAAATTCCAT CACAAAAAAT TCTTGTGTTT 1141 GAACCTTGTT TG //
Primer: SV40pro_F_V2 Sequence file: RDB06265_A7Goc.seq
>6265_6265_A7Go_SV40pro_F_V2_C01_03_ABI08.ab1     1 GTCCAGGCTT TTTTTGGGGA GGGCTAGGCT TTTGCAAAAG CTCCCGGGGG AGCTTGGATA    61 TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT   121 GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA   181 CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG   241 GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG   301 CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT   361 GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT   421 CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG   481 CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT   541 ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC   601 GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GGCGCGCATG CCCGACGGCG AGGATCTCGT   661 CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGGT GGGAAAATGG CCGCTTTTCT   721 GGATTTCATC GACTGTGGCC GGCTGGGTGT GGGCGGACCG CTATCAGGAC ATAGCGTTGG   781 CTACCCCGTG ATATTTGCTG AAGAGCTTGG CGGCGAAATG GGGCTGACCG CTTTCCTCGT   841 GCCTTTTACG GGAATCGCCC GCTCCCGATT TCGCAGCGCG ATCGGCCTTT CTTAATCGCC   901 TTCTTTGGAC GGAGTTTCTT TCTGGAGCGG GGAATTCCTG AGGGTTTCGA AAATGGAACC 961 GAACCAA //

Please visit Sequencing and PCR primers for primer information.

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References

Original, user report and related articles

Resource search

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Link

Other resources in our bank

• human NRF2; NFE2L2

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External Database
Human NRF2; NFE2L2

• in KEGG Gene
• in NCBI Gene

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Reference sequence
  

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