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pCMFlag_hsNR1D1 (#RDB06270)

Expression vector of human NR1D1.

Alternative name SET-0090_6
Clone info. Expression vector of human NR1D1. Expression was confirmed by Western blotting with anti-FLAG antibody.
Comment PCR cloning, forward primer: atgacgaccctggactccaaca; reverse primer: tcactgggcgtccacccgga.
Vector backbone pCMV_S-FLAG (RDB05956) (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Growth conditions 37C, LB+Amp
Gene/insert name Human Rev-ErbA-alpha cDNA
Depositor|Developer DNA Bank, |
 
Remarks, protocol and/or map (pdf) RDB06270.pdf

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution The RECIPIENT agrees to describe RIKEN BRC as the source of the MATERIAL in any publication.
Additional terms and conditions:
Regrding pRc/CMV:
a) The modified pRc/CMV, and their descendants (collectively, LIFE MATERIALS), should be used for pre-clinical/non-human, research use only.
b) LIFE MATERIALS shall not be used for any commercial purposes.
c) LIFE MATERIALS shall not be transferred beyond the specified recipient.
d) Recipient must contact Thermo Fisher Scientific Inc. at outlicense@thermofisher.com for any desired use of LIFE MATERIALS beyond the scope of a) to c) above.
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 発表に際し、理研BRCから入手したことを明記すること。
付加的使用条件：
pRc/CMVについて:
a) The modified pRc/CMV, and their descendants (collectively, LIFE MATERIALS), should be used for pre-clinical/non-human, research use only.
b) LIFE MATERIALS shall not be used for any commercial purposes.
c) LIFE MATERIALS shall not be transferred beyond the specified recipient.
d) Recipient must contact Thermo Fisher Scientific Inc. at outlicense@thermofisher.com for any desired use of LIFE MATERIALS beyond the scope of a) to c) above.

Catalog # Resource name Shipping form Fee
RDB06270 pCMFlag_hsNR1D1 DNA solution JPY 9,460 (not-for-profit academic purpose)
JPY 18,920 (for-profit-research purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCMFlag_hsNR1D1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06270).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13317_A5Ds.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB13317_A5Dsa.seq check
>06270_13317_A5Ds_CMV-Forward_A01_01.ab1
    1 CTTCACTCGA CCATTGACGC AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC
   61 AGAGCTCTCT GGCTAACTAG AGAACCCACT GCTTACTGGC TTATCGAAAT TAATACGACT
  121 CACTATAGGG AGACCCAAGC TGAATTCACC ATGGACTACA AGGACGATGA CGATAAGGCG
  181 GCCATGACGA CCCTGGACTC CAACAACAAC ACAGGTGGCG TCATCACCTA CATTGGCTCC
  241 AGTGGCTCCT CCCCAAGCCG CACCAGCCCT GAATCCCTCT ATAGTGACAA CTCCAATGGC
  301 AGCTTCCAGT CCCTGACCCA AGGCTGTCCC ACCTACTTCC CACCATCCCC CACTGGCTCC
  361 CTCACCCAAG ACCCGGCTCG CTCCTTTGGG AGCATTCCAC CCAGCCTGAG TGATGACGGC
  421 TCCCCTTCTT CCTCATCTTC CTCGTCGTCA TCCTCCTCCT CCTTCTATAA TGGGAGCCCC
  481 CCTGGGAGTC TACAAGTGGC CATGGAGGAC AGCAGCCGAG TGTCCCCCAG CAAGAGCACC
  541 AGCAACATCA CCAAGCTGAA TGGCATGGTG TTACTGTGTA AAGTGTGTGG GGACGTTGCC
  601 TCGGGCTTCC ACTACGGTGT GCACGCCTGC GAGGGCTGCA AGGGCTTTTT CCGTCGGAGC
  661 ATCCAGCAGA ACATCCAGTA CAAAAGGTGT CTGAAGAATG AGAATTGCTC CATCGTCCGC
  721 ATCAATCGCA ACCGCTGCCA GCAATGTCGC TTCAAGAAGT GTCTCTCTGT GGGCATGTCT
  781 CGAGACGCTG TGCGTTTTGG GCGCATCCCC AAACGAGAGA AGCAGCGGAT GCTTGCTGAG
  841 ATGCAGAGTG CCATGAACCT GGCCAACAAC CAGTTGAGCA GCCAGTGCCC GCTGGAGACT
  901 TCACCCACCC AGCACCCCAC CCCAGGCCCC ATGGGCCCCT CGCCACCCCC CTGCTCCGGT
  961 CCCCTCACCC CTGGTGGGCT TCTCCCAGTT TCCACAACAG CTGACGCCCT CCCAGATCCC
 1021 CCAAGCCCTG AGCCCACAGT GGTAGGAATG GTGGATATCC CAGGTGGCCC GGTCCAATCG
1081 AGAAGATCGA TCACTACGAC CCATGAACCA AGCCTGGGGG C
//
Primer: BGH_rev
Sequence file: RDB13317_A5Dsb.seq check
>06270_13317_A5Ds_BGH_rev_B01_04.ab1
    1 GCTACATTAG TGACTATAGA ATAGGGCCCT CTAGATGCAT GCTCGAGCGG CCTCACTGGG
   61 CGTCCACCCG GAAGGACAGC AGCTTCTCGG AATGCATGTT GTTCAGGGTC CGCAGGTCCG
  121 GCAGCTTGAG CAGCAGCTTG GTGAAGCGGG AAGTCTCCAA GGGCCGGTTC TTCAGCACCA
  181 GAGCCCGAAG AGCCCGCAGC AGCGTCTCCT GGAGCTGCTC CACCGAAGCG GAATTCTCCA
  241 TGCCCGAGCG GTCTGCAGAG ACAAGCACCA CCGCGGTGAA GAGGCCCAGC TCCTCCTCGG
  301 TAAGCGCCAG GGAGTTGAGC TTCTCGCTGA AGTCGAACAT GGCACTGAGC AGGTCTCCCA
  361 TGCCCATGGC ACCAAGCTCC TGCAGGCTGT AGGTGGTGCG GCTTAGGAAC ATCACTGTCT
  421 GGTCCTTCAC GTTGAACAAC GAAGCAAAGC GCACCATCAG CACCTCAAAG GTGCCAGCCT
  481 TAAGCAGGGT GACTTGGTCA TGCTGAGAAA GGTCACGGAA GCCCGGGATG TGTTTGGCAA
  541 ACTCTACCAC CTCCCGCACA GCGGGCGTGA AGCTCATGGA GAAATCCTCC CAGATCTCCT
  601 GCACCGTTCG CCCACTGCGT CCATGCGGGT ACATGTTCAT AGGACATGCC AGCAGAACAT
  661 TCTTTGAGTT GCCCTGCCGG GGACTGTTGG CAGGTGCCTT GCCTTCTGGG GCTGCATACA
  721 CGTGGGTGGG GCATAGACGG TGCCCGTTGC TGTTGGACTG GTGGCAGCTG TGGTGTGCAG
  781 GGCCAGGAGG CCAGGTGGGA GGGTAGGAGG AGGGAGCCTG GCGCAGACCA TTTAGGGCCT
  841 CGTTATGACG CTGGGCAGCC AAGGTGTTGT TGTCATTGGG GGGCAGGTGG GCAGCCCTGA
  901 TTTTCCCAGC GATGTGGGGG TGGTGGCTGG AGGGCTACCT GATGCATGGT TTGGCATTGA
  961 AAGTTTGCCA GGTGAGCTGC CCAGCTTTGT CATGGCGTAG GTGAAGATCT CTCGATGGGC
 1021 CCGGGCCACC TGCGATATCA CATTCTTCAC CTGTTGGGCC TTCATGACCT GGGGCAATCC
1081 TGGCAGTCGA TCAGCTGATG TGAACTGTAG CAGGCCGAC
//
Primer: SV40pro_F_V2
Sequence file: RDB13317_A5Dsc.seq check
>06270_13317_A5Ds_SV40pro_F_V2_C01_07.ab1
    1 GGAGCTTTTT GGAGCTAGGC TTTTGCAAAA GCTCCCGGGA GCTTGGATAT CCATTTTCGG
   61 ATCTGATCAA GAGACAGGAT GAGGATCGTT TCGCATGATT GAACAAGATG GATTGCACGC
  121 AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT GACTGGGCAC AACAGACAAT
  181 CGGCTGCTCT GATGCCGCCG TGTTCCGGCT GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT
  241 CAAGACCGAC CTGTCCGGTG CCCTGAATGA ACTGCAGGAC GAGGCAGCGC GGCTATCGTG
  301 GCTGGCCACG ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC GTTGTCACTG AAGCGGGAAG
  361 GGACTGGCTG CTATTGGGCG AAGTGCCGGG GCAGGATCTC CTGTCATCTC ACCTTGCTCC
  421 TGCCGAGAAA GTATCCATCA TGGCTGATGC AATGCGGCGG CTGCATACGC TTGATCCGGC
  481 TACCTGCCCA TTCGACCACC AAGCGAAACA TCGCATCGAG CGAGCACGTA CTCGGATGGA
  541 AGCCGGTCTT GTCGATCAGG ATGATCTGGA CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA
  601 ACTGTTCGCC AGGCTCAAGG CGCGCATGCC CGACGGCGAG GATCTCGTCG TGACCCATGG
  661 CGATGCCTGC TTGCCGAATA TCATGGTGGA AAATGGCCGC TTTTCTGGAT TCATCGACTG
  721 TGGCCGGCTG GGTGTGGCGG ACCGCTATCA GGACATAGCG TTGGCTACCC GTGATATTGC
  781 TGAAGAGCTT GGCGGCGAAT GGGCTGACCG CTTCCTCGTG CTTTACGGTA TCGCCGCTCC
  841 CGATTCGCAG CGCATCGCCT TCTATCGCCT TCTTGACGAG TTCTTCTGAG CGGGACTCTG
  901 GGGTTCGAAA TGACCGACCA AGCGACGCCC AACCCTGCCA TCACGAGATT TCGATTCCAC
  961 CCGCCGCCTT CTATGAAGGT TGGGCTTCGG GATTCGTTTC CGGACGCCGG GCTGGATGGA
 1021 TTCCTCAGCG CGGGGATCTC ATGCTGGAGT CTCGGCCAAC CCCACTTATA TGCAGCTATA
1081 TGGTACTATT AACGCCATAG CCAGTC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles