Please review the QC test results indicated by 
icon below as well as clone information before placing your order.
提供案内 (日本国内) 
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Please review the QC test results indicated by icon below as well as clone information before placing your order.
icon below as well as clone information before placing your order.
pCMFlag_hsXBP1 (#RDB06272)
Expression vector of human XBP1.
| Alternative name | SET-0107_16 |
|---|---|
| Clone info. | Expression vector of human XBP1. Expression was confirmed by Western blotting with anti-FLAG antibody. |
| Comment | Derived from Sugano BC000938. PCR cloning, forward primer: gtggtggtggcagccgcgccga; reverse primer: ttagttcattaatggcttccagcttg. |
| Vector backbone | pCMV_S-FLAG (RDB05956) (Plasmid) |
| Size of vector backbone | 5.5 kb |
| Selectable markers | Amp^r (E. coli), Neo^r (mammalian cell) |
| Growth conditions | 37C, LB+Amp |
| Gene/insert name | Human XBP1 cDNA |
| Depositor|Developer | DNA Bank, | |
| Remarks, protocol and/or map (pdf) | RDB06272.pdf |
|---|
Distribution information
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Ordering forms | Order form [Credit Card Payment  ] [Bank Transfer Payment] [Example of order form ]MTA, for use for not-for-profit academic purpose [Word ] [Example of MTA ]MTA, for use for for-profit purpose [Word ]Please visit Information of Request for Distribution.[link] |
|---|---|
| Terms and conditions for distribution | The RECIPIENT agrees to describe RIKEN BRC as the source of the MATERIAL and cite Gene 200, 149-156, 1997 in any publication. Additional terms and conditions: Regrding pRc/CMV: a) The modified pRc/CMV, and their descendants (collectively, LIFE MATERIALS), should be used for pre-clinical/non-human, research use only. b) LIFE MATERIALS shall not be used for any commercial purposes. c) LIFE MATERIALS shall not be transferred beyond the specified recipient. d) Recipient must contact Thermo Fisher Scientific Inc. at outlicense@thermofisher.com for any desired use of LIFE MATERIALS beyond the scope of a) to c) above. |
[open/close]| 必要書類 | 提供依頼書 [依頼書の記入例 ]提供同意書 (MTA, 非営利学術目的用)[Word ] [MTAの記入例 ]提供同意書 (MTA, 営利目的用)[Word ]手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
|---|---|
| MTAに書く使用条件 | 発表に際し、理研BRCから入手したことを明記し、指定する文献(Gene, 200, 149-156 (1997))を引用すること。 付加的使用条件： pRc/CMVについて: a) The modified pRc/CMV, and their descendants (collectively, LIFE MATERIALS), should be used for pre-clinical/non-human, research use only. b) LIFE MATERIALS shall not be used for any commercial purposes. c) LIFE MATERIALS shall not be transferred beyond the specified recipient. d) Recipient must contact Thermo Fisher Scientific Inc. at outlicense@thermofisher.com for any desired use of LIFE MATERIALS beyond the scope of a) to c) above. |
| Catalog # | Resource name | Shipping form | Fee |
|---|---|---|---|
| RDB06272 | pCMFlag_hsXBP1 | DNA solution |
JPY 9,460 (not-for-profit academic purpose) JPY 18,920 (for-profit-research purpose) plus cost of shipping containers, dry ice (if required) and shipping charge |
icon below as well as clone information before placing your order.How to cite this biological resource
Materials & Methods section:
| The pCMFlag_hsXBP1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06272). |
Reference section:
Further references such as user reports and related articles (go to bottom)
QC test results
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
| Test sheet | RDB06272_A3Ir.pdf |
|---|
Nucleotide sequence of a portion of this resource (if available).
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Primer: Sequence file: RDB06272_A3Ira.seq
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>06272_06272_A3Ir_CMV-Foward_C11_08.ab1 1 TCTTCCCACT CCGCCCCATT GACGCAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA 61 TAAGCAGAGC TCTCTGGCTA ACTAGAGAAC CCACTGCTTA CTGGCTTATC GAAATTAATA 121 CGACTCACTA TAGGGAGACC CAAGCTGAAT TCACCATGGA CTACAAGGAC GATGACGATA 181 AGGCGGCCGT GGTGGTGGCA GCCGCGCCGA ACCCGGCCGA CGGGACCCCT AAAGTTCTGC 241 TTCTGTCGGG GCAGCCCGCC TCCGCCGCCG GAGCCCCGGC CGGCCAGGCC CTGCCGCTCA 301 TGGTGCCAGC CCAGAGAGGG GCCAGCCCGG AGGCAGCGAG CGGGGGGCTG CCCCAGGCGC 361 GCAAGCGACA GCGCCTCACG CACCTGAGCC CCGAGGAGAA GGCGCTGAGG AGGAAACTGA 421 AAAACAGAGT AGCAGCTCAG ACTGCCAGAG ATCGAAAGAA GGCTCGAATG AGTGAGCTGG 481 AACAGCAAGT GGTAGATTTA GAAGAAGAGA ACCAAAAACT TTTGCTAGAA AATCAGCTTT 541 TACGAGAGAA AACTCATGGC CTTGTAGTTG AGAACCAGGA GTTAAGACAG CGCTTGGGGA 601 TGGATGCCCT GGTTGCTGAA GAGGAGGCGG AAGCCAAGGG GAATGAAGTG AGGCCAGTGG 661 CCGGGTCTGC // |
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Primer: Sequence file: RDB06272_A3Irb.seq
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>06272_06272_A3Ir_Sp6_D11_11.ab1 1 CCATATAATC CATCCTCGAG CGGCTTAGTT CATTAATGGC TTCCAGCTTG GCTGATGACG 61 TCCCCACTGA CAGAGAAAGG GAGGCTGGTA AGGAACTGGG TCCTTCTGGG TAGACCTCTG 121 GGAGCTCCTC CAGGCTGGCA GGCTCTGGGG AAGGGCATTT GAAGAACATG ACTGGGTCCA 181 AGTTGTCCAG AATGCCCAAC AGGATATCAG ACTCTGAATC TGAAGAGTCA ATACCGCCAG 241 AATCCATGGG GAGATGTTCT GGAGGGGTGA CAACTGGGCC TGCACCTGCT GCAGAGGTGC 301 ACGTAGTCTG AGTGCTGCGG ACTCAGCAGA CCCGGCCACT GGCCTCACTT CATTCCCCTT 361 GGCTTCCGCC TCCTCTTCAG CAACCAGGGC ATCCATCCCC AAGCGCTGTC TTAACTCCTG 421 GTTCTCAACT ACAAGGCCAT GAGTTTTCTC TCGTAAAAGC TGATTTTCTA GCAAAAGTTT 481 TTGGTTCTCT TCTTCTAAAT CTACCACTTG CTGTTCCAGC TCACTCATTC GAGCCTTCTT 541 TCGATCTCTG GCAGTCTGAG CTGCTACTCT GTTTTTCAGT TTCCTCCTCA GCGCCTTCTC 601 CTCGGGGCTC AGGTGCGTGA GGCGCTGTCG CTTGCGCGCC TGGGGCAGCC CCCCGCTCGC 661 TGCCTCCGGG CTGGC // |
Please visit 
Sequencing and PCR primers for primer information.
References
Original, user report and related articles
Resource search
Link
Other resources in our bankExternal Database
Human XBP1
Reference sequence
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