Please review the QC test results indicated by icon below as well as clone information before placing your order.

pCMFlag_hsNR0B1 (#RDB06297)

Expression vector of human ACH.

Alternative name	SET-0026_126
Clone info.	Expression vector of human ADR. Expression was confirmed by Western blotting with anti-FLAG antibody.
Comment	Derived from Sugano BC011564. PCR cloning, forward primer: gcgggcgagaaccaccagtg; reverse primer: ttatatctttgtacagagcatttcca.
Vector backbone	pCMV_S-FLAG (RDB05956) (Plasmid)
Size of vector backbone	5.5 kb
Selectable markers	Amp^r (E. coli), Neo^r (mammalian cell)
Growth conditions	37C, LB+Amp
Gene/insert name	Human DAX1;ACH cDNA
Depositor|Developer	DNA Bank, |

 

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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] MTA, for use for for-profit purpose [Word] Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution

The RECIPIENT agrees to describe RIKEN BRC as the source of the MATERIAL and cite Gene 200, 149-156, 1997 in any publication. Additional terms and conditions: Regrding pRc/CMV: a) The modified pRc/CMV, and their descendants (collectively, LIFE MATERIALS), should be used for pre-clinical/non-human, research use only. b) LIFE MATERIALS shall not be used for any commercial purposes. c) LIFE MATERIALS shall not be transferred beyond the specified recipient. d) Recipient must contact Thermo Fisher Scientific Inc. at outlicense@thermofisher.com for any desired use of LIFE MATERIALS beyond the scope of a) to c) above.

提供案内 (日本国内) [open/close]

Catalog #	Resource name	Shipping form	Fee
RDB06297	pCMFlag_hsNR0B1	DNA solution	JPY 9,460 (not-for-profit academic purpose) JPY 18,920 (for-profit-research purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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How to cite this biological resource

Materials & Methods section:

The pCMFlag_hsNR0B1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06297).

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB06297_A3Irp2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward Sequence file: RDB06297_A3Ira.seq
>06297_06297_A3Ir_CMV-Foward_E11_14.ab1     1 TGTATCAACT TCCGCCCATT GACGCAAATG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT    61 ATAAGCAGAG CTCTCTGGCT AACTAGAGAA CCCACTGCTT ACTGGCTTAT CGAAATTAAT   121 ACGACTCACT ATAGGGAGAC CCAAGCTGAA TTCACCATGG ACTACAAGGA CGATGACGAT   181 AAGGCGGCCG CGGGCGAGAA CCACCAGTGG CAGGGCAGCA TCCTCTACAA CATGCTTATG   241 AGCGCGAAGC AAACGCGCGC GGCTCCTGAG GCTCCAGAGA CGCGGCTGGT GGATCAGTGC   301 TGGGGCTGTT CGTGCGGCGA TGAGCCCGGG GTGGGCAGAG AGGGGCTGCT GGGCGGGCGG   361 AACGTGGCGC TCCTGTACCG CTGCTGCTTT TGCGGTAAAG ACCACCCACG GCAGGGCAGC   421 ATCCTCTACA GCATGCTGAC GAGCGCAAAG CAAACGTACG CGGCACCGAA GGCGCCCGAG   481 GCGACGCTGG GTCCGTGCTG GGGCTGTTCG TGCGGCTCTG ATCCCGGGGT GGGCAGAGCG   541 GGGCTTCCGG GTGGGCGGCC CGTGGCACTC CTGTACCGCT GCTGCTTTTG TGGTGAAGAC   601 CACCCGCGGC AGGGCAGCAT CCTCTACAGC TTGCTCACTA GCTCAAAGCA AACGCACGTG   661 GCTCCGGCAG CGCCCGAGGC ACGGCCAGGG GGCGCGTGGT GGGACCGCTC CTACTTCGCG   721 CAGAGGCCAG GGGGTAAAGA GGCGCTACCA GGCGGGCGGG CCACGGCGCT TCTGTACCGC   781 TGCTGCTTTT GCGGTGAAGA CCACCCGCAG CAGGGCAGCA CCCTCTACTG CGTGCCCACG   841 AGCACAAATC AAGCGCAGGC GGCTCCGGAG GAGCGGCCGA GGGCCCCCTG GTGGGACACC   901 TCCTCTGGTG CGCTGCGGCC GGTGGCGCTC AAGAGTCCAC AGGTGGTCTG CGAGGCAGCC   961 TCAGCGGGCC TGTTGAAGAC GCTGCGCTTC GTCAAGTACT TGCCCTGCTT CCAGGTGCTG  1021 CCCCTGGACC AGCAGCTGGG TGCTGGGTGC GCAACTGCTG GCGTCCCTGC TCATGCTGAG  1081 CTGGGCCCAG GACCGCTTGC AGATCGAGAC CTGTGAGTCT CGGAGCTCAG CATGCTGCCA 1141 GAAAAGAAAT TCCCTTCCAA CCGAC //
Primer: SP6 Sequence file: RDB06297_A3Irb.seq
>06297_06297_A3Ir_Sp6_F11_17.ab1     1 CCTTTAGTCA TGCCTCGAGC GGCCTTATAT CTTTGTACAG AGCATTTCCA GCATCATATC    61 ATCCATGCTG ACTGTGCCGA TGATGGGCCT GAAGAACAGT TCAGCAATGA CATTGGCATT   121 GATGAATCTC AGCAGGAAAA GGGTACTATT AAGTTCGATG AATCTGTCAT GGGGCCCTTG   181 GTGCGTCATC CTGGTGTGTT CACTGAGTAT TTGCTGAGTT CCCCACTGGA GTCCCTGAAT   241 GTACTTCACG CACTGCAGGC CCGGCACGTC CGGGTTAAAG AGCACGGTCC CCTTGAGGTA   301 GGCGTACTCC TTGGTACTGA TGTTCAGACT CCAGCATTTG GAAAGAAAGC ACTTGATGGC   361 TTGGACCTGG GAGGCGGAGG GCACCTTCCT GGCCTCCGCC GGCGGTGCCA AATGGTGCTG   421 CAGCGTGGGC ACGGGCAGTG GCTCGTTGCC CCCGGTCTCC CGCCGCCTGG TGGTGAGGAT   481 CTTCTGCAGC ATGCTGGGCT CCGAGACTTC CACAGTCTCG AACTGCAAGC GGTCCTGGGC   541 CAGCTCAAGC ATGAGCAGGG ACGCCCAGCA GTTGCGCACC AGCACCAGCT GCTGGTCCAG   601 GGGCAGCACC TGGAAGCAGG GCAAGTACTT GACGAAGCGC AGCGTCTTCA ACAGGCCCGC   661 TGAGGCTGCC TCGCAGACCA CCTGTGGACT CTTGAGCGCC ACCGGCCGCA GCGCACCAGA   721 GGAGGTGTCC CACCAGGGGG CCCTCGGCCG CTCCTCCGGA GCCGCCTGCG CTTGATTTGT   781 GCTCGTGGGC ACGCAGTAGA GGGTGCTGCC CTGCTGCGGG TGGTCTTCAC CGCAAAAGCA   841 GCAGCGGTAC AGAAGCGCCG TGGCCCGCCC GCCTGGTAGC GCCTCTTTAC CCCCTGGCCT   901 CTGCGCGAAG TAGGAGCGGT CCCACCACGC GCCCCCTGGC CGTGCCTCGG GCGCTGCCGG   961 AGCCACGTGC GTTTGCTTTG AGCTAGTGAG CAAGCTGTAG AGGATGCTGC CCTGCCGCGG  1021 GTGGGTCTTC ACCACAAAAG CAGCAGCGGG TACAGGGAGT GCCACGGGGC CCGCCCACCC  1081 CGGGAAGCCC CGCTCTGCCC CAACCCCGGG ATTCAGAGCG CACGAACCAG CTTCAGCACG 1141 GGAACCCAGG CCGAATCGTC ATG //
Primer: SV40pro_F_V2 Sequence file: RDB06297_A3Irc.seq
>06297_06297_A3Ir_SV40pro_F_V2_G02_07_ABI08.ab1     1 GGCACAACCT TTTTTTGGGG GGGGCTTAGG GCTTTTGCAA AAAGCTCCCG GGGAGCTTGG    61 ATATCCATTT TCGGATCTGA TCAAGAGACA GGATGAGGAT CGTTTCGCAT GATTGAACAA   121 GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG   181 GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC   241 CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA ATGAACTGCA GGACGAGGCA   301 GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC   361 ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA   421 TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT   481 ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA   541 CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC TGGACGAAGA GCATCAGGGG   601 CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGCGCA TGCCCGACGG CGAGGATCTC   661 GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG TGGAAAATGG CCGCTTTTCT   721 GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT ATCAGGACAT AGCGTTGGCT   781 ACCCGTGATA TTGCTGAAGA GCTTGGCGGG CGAAATGGGC CTGACCGCTT CCTCGTGCTT   841 TACGGTATCG CCGCTCCCGA TTCGCAGCGC ATCGCCTTTC TATCGCCTTT CTTGACGAGG   901 TTCTTCTGAG CGGGACTCTG GGGGTTCGAA ATGACCGACC AAGCGACGCC CCAACCTGGC   961 CCATCACGAG ATTTCGATCC ACCCGCCGCC CTTCTATGAA AAGGTTGGGG CTTCGGAAAT  1021 CGTTTTCCGG GACGCCCGGC CTGGGATGAT CCCTCCCAGC GCGGGGATCT CATGGCTGGG  1081 AGTTCTTCCG CCCAACCCCG AACTGCTTTA TTGCCAGCCT TATAAATGGG TTACAAGTAA  1141 AACATAGCCA TCCACCAAAG TTCACCAAAT CAAGCATTTT TTCACTTGCG ATTCCTAGTT 1201 TGGTGGTATT TGTCCCAAAA CTCTCCATCT ACGAACTTGT AGAATCAC //

Please visit Sequencing and PCR primers for primer information.

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References

Original, user report and related articles

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Other resources in our bank

• human DAX1;ACH

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External Database
Human DAX1;ACH

• in KEGG Gene
• in NCBI Gene

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Reference sequence
  

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