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pET_HisFLAG-hsATF-2 (#RDB06321)

Expression vector of human ATF-2 tagged with His- and FLAG- epitope

Clone info. Expression vector of human ATF-2 tagged with His- and FLAG- epitope.
Comment Nucleotide substitution T1072G (Phe358Val) to NM_001880.
Vector backbone pET15b (Plasmid)
Size of vector backbone 4.8 kb
Selectable markers Amp^r
Gene/insert name Human CREB2; ATF2 cDNA
Depositor|Developer Yokoyama, Kazunari |
 

Distribution information

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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06321 pET_HisFLAG-hsATF-2 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pET_HisFLAG-hsATF-2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06321).

Reference section:

Murata T., Shinozuka Y., Obata Y., Yokoyama K.K., Phosphorylation of two eukaryotic transcription factors, Jun dimerization protein 2 and activation transcription factor 2, in Escherichia coli by Jun N-terminal kinase 1. Anal. Biochem., 376, 115-121 (2008). PMID 18307971. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13434_A5Fb.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp
Sequence file: RDB13434_A5Fba.seq check
>06321_13434_A5Fb_T7_20bp_G08_20.ab1
    1 TGTCTCCGTC TAGATAATTT TGTTTAACTT TAAGAAGGAG ATATACCATG GGCAGCAGCC
   61 ATCATCATCA TCATCACAGC AGCGGCCTGG TGCCGCGCGG CAGCCATATG GACTACAAGG
  121 ACGATGACGA TAAGGCGGCC ATGAAATTCA AGTTACATGT GAATTCTGCC AGGCAATACA
  181 AGGACCTGTG GAATATGAGT GATGACAAAC CCTTTCTATG TACTGCGCCT GGATGTGGCC
  241 AGCGTTTTAC CAACGAGGAT CATTTGGCTG TCCATAAACA TAAACATGAG ATGACACTGA
  301 AATTTGGTCC AGCACGTAAT GACAGTGTCA TTGTGGCTGA TCAGACCCCA ACACCAACAA
  361 GATTCTTGAA AAACTGTGAA GAAGTGGGTT TGTTTAATGA GTTGGCGAGT CCATTTGAGA
  421 ATGAATTCAA GAAAGCTTCA GAAGATGACA TTAAAAAAAT GCCTCTAGAT TTATCCCCTC
  481 TTGCAACACC TATCATAAGA AGCAAAATTG AGGAGCCTTC TGTTGTAGAA ACAACTCACC
  541 AGGATAGTCC TTTACCTCAC CCAGAGTCTA CTACCAGTGA TGAGAAGGAA GTACCATTGG
  601 CACAAACTGC ACAGCCCACA TCAGCTATTG TTCGTCCAGC ATCATTACAG GTTCCCAATG
  661 TGCTGCTTAC AAGTTCTGAC TCAAGTGTAA TTATTCAGCA GGCAGTACCT TCACCAACCT
  721 CAAGTACTGT AATCACCCAG GCACCATCCT CTAACAGGCC AATTGTCCCT GTACCAGGCC
  781 CATTTCCTCT TCTGTTACAT CTTCCTAATG GACAAACCAT GCCTGTTGCT ATTCCTGCAT
  841 CAATTACAAG TTCTAATGTG CATGTTCCAG CTGCAGTCCC ACTCGTTCGA CCAGTCACCA
  901 TGGTGCCTAG TGTTCCAGGA ATCCCAGGTC CTTCCTCTCC CCAACCAGTA CAGTCAGAAG
  961 CAAAAATGAG ATTAAAAGCT GCTTTGACCC AGCACATCCT CCAGTTACCC AATGGTGATA
 1021 CTGTCAACGT CATGGTAGCG CATTGATTAA GAACTCAGTC AGAAGGATCT CGACGCAGTC
1081 ATTAACGACA GCAAGCCACA TTCAACTAAC AGA
//
Primer: T7 terminator
Sequence file: RDB13434_A5Fbb.seq check
>06321_13434_A5Fb_T7terminator_H08_23.ab1
    1 ACGCATCTTT CGGGCTTTGT TAGCAGCCGG ATCCCCTCAA CTTCCTGAGG GCTGTGACTG
   61 GGAGGAAGGA GCCATAACGA TCTGTGAAAG AGCAGGCTCT GTACTCTGGT CCGCCATCTG
  121 GGTGAGGACT GAAGTGGCTA CAGCTTCTGC CTTGGAGGTT GAACTGACTC CATTGGATGT
  181 GCTGACCGAA CTATGCTGTA TAGCTTCTGT ATGTGGACTA CTCGGCACTG AAATGTCTTC
  241 TGAACTATCA TCTTTATCAG CAGTATGATA GCCAGATTTC TTCTGCATGG CGGTTACAGG
  301 GCAATCTTTA TGAGCCAGAA GAAGCTGTTT CAGCTGTGCC ACTTCATTTC TCAGCAGGGT
  361 GACTTCACTC TGCAGCTGAC CATTTAATGA ACTCAAGTCT TCAGCTTTCT TCTCTAAAGA
  421 CTGAACCCAG ACTTTCCTTT TTTGTCGGCA TCTTGAAGCT GCTGCTCTAT TTCGCTCTAA
  481 AACCTTTCTC CTTTTTTCAT CAGGATCTTC GTTAGCTGCT CTTCTCCGAC GACCACTTGT
  541 ACTTTGGGTC TGTGGAGTTG TGTGAGCTGG AGAAGCCGGA GTTTCTGTAG TGGATGTGGC
  601 TGGCTGTTGT AATGACTGCG GTCGAGATTC CTCTGACTGA GTCCTAACCA ATCCGCTACC
  661 ATGACCTTTG ACAGTATCAC CATTGGTAAC TGGAGGATGT TGCTGGGTCA AAGCAGCTTT
  721 TAATCTCATT TTTGCTTCTG ACTGTACTGG TTGGGGAGAG GAAGGACCTG GGATTCCTGG
  781 AACACTAGGC ACCATGGTGA CTGGTCGAAC GAGTGGGACT GCAGCTGGAA CATGCACATT
  841 AGAACTTGTA ATTGATGCAG GAATAGCAAC AGGCATGGTT TGTCCATTAG GAAGATGTAA
  901 CAGAAGAGGA AATGGGGCCT GGTACAGGGA CAATTGGCCT GTTAGAGGAT GGTGCCTGGG
  961 TGATTACAGT ACTTGAGGTT GGGTGAAAGG TACTGCCTGC TGAATAATTA CACTTTGAGT
 1021 CAGAACTTTG TAAGCAGCAC ATTTGCGAAC CTGTAATGAT GGCTGACGGA ACATAGCTGA
 1081 TGTGGCTGTG CAGCTTGTGC TATGGCTACG TACGTTCTCA ATTCACCTGG CTAAGTTTAG
1141 GAC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Murata T., Phosphorylation of two eukaryotic transcription factors, Jun dimerization protein 2 and activation transcription factor 2, in Escherichia coli by Jun N-terminal kinase 1. Anal. Biochem., 376, 115-121 (2008). PMID 18307971. [link to RRC of NBRP]