Please review the QC test results indicated by icon below as well as clone information before placing your order.

pCMFlag_hsFLI1 (#RDB06375)

Expression vector of human FLI1.

Alternative name	SET-0170_21
Clone info.	Expression vector of human FLI1. Expression was confirmed by Western blotting with anti-FLAG antibody.
Comment	Derived from Sugano BC001670. PCR cloning, forward primer: gacgggactattaaggaggc; reverse primer: ctagtagtagctgcctaagtgtgaag.
Vector backbone	pCMV_S-FLAG (RDB05956) (Plasmid)
Size of vector backbone	5.5 kb
Selectable markers	Amp^r (E. coli), Neo^r (mammalian cell)
Growth conditions	37C, LB+Amp
Gene/insert name	Human FLI-1 cDNA
Depositor|Developer	DNA Bank, |

 

Remarks, protocol and/or map (pdf)	RDB06375.pdf

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] MTA, for use for for-profit purpose [Word] Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution

The RECIPIENT agrees to describe RIKEN BRC as the source of the MATERIAL and cite Gene 200, 149-156, 1997 in any publication. Additional terms and conditions: Regrding pRc/CMV: a) The modified pRc/CMV, and their descendants (collectively, LIFE MATERIALS), should be used for pre-clinical/non-human, research use only. b) LIFE MATERIALS shall not be used for any commercial purposes. c) LIFE MATERIALS shall not be transferred beyond the specified recipient. d) Recipient must contact Thermo Fisher Scientific Inc. at outlicense@thermofisher.com for any desired use of LIFE MATERIALS beyond the scope of a) to c) above.

提供案内 (日本国内) [open/close]

Catalog #	Resource name	Shipping form	Fee
RDB06375	pCMFlag_hsFLI1	DNA solution	JPY 9,460 (not-for-profit academic purpose) JPY 18,920 (for-profit-research purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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How to cite this biological resource

Materials & Methods section:

The pCMFlag_hsFLI1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06375).

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15915_A8Dgp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward Sequence file: RDB15915_A8Dga.seq
>06375_15915_A8Dg_1_CMV-Forward_A01_01_ABI08.ab1     1 TTTTTAACTC CGCCCCATTG ACGCAATGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT    61 AAGCAGAGCT CTCTGGCTAA CTAGAGAACC CACTGCTTAC TGGCTTATCG AAATTAATAC   121 GACTCACTAT AGGGAGACCC AAGCTGAATT CACCATGGAC TACAAGGACG ATGACGATAA   181 GGCGGCCGAC GGGACTATTA AGGAGGCTCT GTCGGTGGTG AGCGACGACC AGTCCCTCTT   241 TGACTCAGCG TACGGAGCGG CAGCCCATCT CCCCAAGGCC GACATGACTG CCTCGGGGAG   301 TCCTGACTAC GGGCAGCCCC ACAAGATCAA CCCCCTCCCA CCACAGCAGG AGTGGATCAA   361 TCAGCCAGTG AGGGTCAACG TCAAGCGGGA GTATGACCAC ATGAATGGAT CCAGGGAGTC   421 TCCGGTGGAC TGCAGCGTTA GCAAATGCAG CAAGCTGGTG GGCGGAGGCG AGTCCAACCC   481 CATGAACTAC AACAGCTATA TGGACGAGAA GAATGGCCCC CCTCCTCCCA ACATGACCAC   541 CAACGAGAGG AGAGTCATCG TCCCCGCAGA CCCCACACTG TGGACACAGG AGCATGTGAG   601 GCAATGGCTG GAGTGGGCCA TAAAGGAGTA CAGCTTGATG GAGATCGACA CATCCTTTTT   661 CCAGAACATG GATGGCAAGG AACTGTGTAA AATGAACAAG GAGGACTTCC TCCGCGCCAC   721 CACCCTCTAC AACACGGAAG TGCTGTTGTC ACACCTCAGT TACCTCAGGG AAAGTTCACT   781 GCTGGCCTAT AATACAACCT CCCACACCGA CCAATCCTCA CGATTGAGTG TCAAAGAAGA   841 CCCTTCTTAT GACTCAGTCA GAAGAGGAGC TTGGGGCAAT AACATGAATT CTGGCCTCAA   901 CAAAAGTCCT CCCCTTGGAG GGGCACAAAC GATCAGTAAG AATACAGAGC AACGGCCCCA   961 GCCAGATCCG TATCAGATCC TGGGCCCGAC CAGCAGTCGC CCTAGCCCAA CCCTGGAAGC  1021 GGGCAGATCC CAGCTGTGGC AATTTCCTCC TGAGCTGCTC TCCGACAGCG CCACGCAGGC  1081 TGTATCACCT GCGAGGGCAC GACGAGAAGT CCAAATTGAC GGAACCCCGA TGAGGTGACC 1141 AAGGCCGCCC G //
Primer: hsFLI1_F2 Sequence file: RDB15915_A8Dgb.seq
>06375_15915_A8Dg_1_hsFLI1_F2_A02_02_ABI24.ab1     1 ACGGGGGCCC CAAGCCAGAT CCGTATCAGA TCCTGGGCCC GACCAGCAGT CGCCTAGCCA    61 ACCCTGGAAG CGGGCAGATC CAGCTGTGGC AATTCCTCCT GGAGCTGCTC TCCGACAGCG   121 CCAACGCCAG CTGTATCACC TGGGAGGGGA CCAACGGGGA GTTCAAAATG ACGGACCCCG   181 ATGAGGTGGC CAGGCGCTGG GGCGAGCGGA AAAGCAAGCC CAACATGAAT TACGACAAGC   241 TGAGCCGGGC CCTCCGTTAT TACTATGATA AAAACATTAT GACCAAAGTG CACGGCAAAA   301 GATATGCTTA CAAATTTGAC TTCCACGGCA TTGCCCAGGC TCTGCAGCCA CATCCGACCG   361 AGTCGTCCAT GTACAAGTAC CCTTCTGACA TCTCCTACAT GCCTTCCTAC CATGCCCACC   421 AGCAGAAGGT GAACTTTGTC CCTCCCCATC CATCCTCCAT GCCTGTCACT TCCTCCAGCT   481 TCTTTGGAGC CGCATCACAA TACTGGACCT CCCCCACGGG GGGAATCTAC CCCAACCCCA   541 ACGTCCCCCG CCATCCTAAC ACCCACGTGC CTTCACACTT AGGCAGCTAC TACTAGGGCC   601 GCTCGAGCAT GCATCTAGAG GGCCCTATTC TATAGTGTCA CCTAAATGCT AGAGCTCGCT   661 GATCAGCCTC GACTGTGCCT TCTAGTTGCC AGCCATCTGT TGTTTGCCCC TCCCCCGTGC   721 CTTCCTTGAC CCTGGAAGGT GCCACTCCCA CTGTCCTTTC CTAATAAAAT GAGGAAATTG   781 CATCGCATTG TCTGAGTAGG TGTCATTCTA TTCTGGGGGG TGGGGGTGGG GCAGGACAGC   841 AAGGGGGAGG ATTGGGAAGA CAATAGCAGG CATGCTGGGG ATGCGGTGGG CTCTATGGCT   901 TCTGAGGCGG AAAGAACCAG CTGGGGCTCG AGGGGGGATC CCCACGCGCC CTGTAGCGGC   961 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC  1021 CTAGCGCCCG CTCTTTCGCT TTTCTTCCCT TCCTTTCTCG CCACGTCGCC GGCTTCCCCG  1081 TCAGCTCTAA TCGGGCTCCT TAAGGATCCG ATTATGCTTT ACGGCACCTC GACCTCCTAA 1141 AAACTGAATA AGGGTGGTAT ATGAT //
Primer: SV40pro_F Sequence file: RDB15915_A8Dgc.seq
>06375_15915_A8Dg_1_SV40pro_F_B01_02_ABI08.ab1     1 TGGGGGCACT TCGGGACCGG CTTGGGGTAG CATTCAGAGT AGTGAGGGAG GCTTTTTTGG    61 AGGCCTAGGC TTTTGCAAAA AGCTCCCGGG AGCTTGGATA TCCATTTTCG GATCTGATCA   121 AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC   181 GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC   241 TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA   301 CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC   361 GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT   421 GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA   481 AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC   541 ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT   601 TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC   661 CAGGCTCAAG GCGCGCATGC CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG   721 CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT   781 GGGTGTGGCG GACCGCTATC AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT   841 TGGCGGCGAA TGGGGCTGAC CGCTTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG   901 CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT GAGCGGGGAC TCTGGGGTTC   961 GAAATGACCG ACCAAGCGAC GCCCAACCTG CCATCACGAG ATTTCGATTC CACCGCCGCC  1021 TTCTATGAAG GTGGGCTTCG GAATCGTTTT CCGGGACGCC GGCTTGGATG AATCCTCCAG  1081 CGCGGGGGAA TCTCATGCTG GAGGTTCTTC GCCCCACCCT CAACTGTTAT GGCCAGGCTG 1141 ATATGGTTAC AAGTAAGGCA AATAGGCCAT TCACCAC //

Please visit Sequencing and PCR primers for primer information.

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References

Original, user report and related articles

Resource search

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Link

Other resources in our bank

• human FLI-1

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External Database
Human FLI-1

• in KEGG Gene
• in NCBI Gene

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Reference sequence
  

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