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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

CSII-EF-mRFP1 (#RDB06387)

Lentivirus vector plasmid of mRFP1 driven by EF-1 alpha promoter.

Clone info. Lentivirus vector plasmid of mRFP1 driven by EF-1 alpha promoter.
Comment Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394).
Vector backbone CSII-EF-MCS (RDB04378) (Plasmid)
Selectable markers Ampicillin (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Growth conditions 37C, LB+Amp
Gene/insert name Discosoma sp. RFP cDNA
Depositor|Developer Miyoshi, Hiroyuki |
 
Sequence RDB06387z.seq
Remarks, protocol and/or map (pdf) RDB06387.pdf

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Ordering instruction.[link] 
Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
Please contact us. We will provide you with appropriate documents.
This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
備考 【使用上の注意】このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06387 CSII-EF-mRFP1 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The CSII-EF-mRFP1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06387).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB19559_B2C2p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F (Pr0168)
Region: EF1 alpha pro,insert 5'
Sequence file: RDB19559_B2C2a.seq check
>06387_19559_B2C2_1_EF1a-C_F_A02_02_ABI24.ab1
    1 NNNNNNNNNN NNTTTTTGAN TTTGGNNCTT GGTTNNTTCT CAAGCCTCAG ACAGTGGTTC
   61 AAAGTTTTTT TCTTCCATTT CAGGTGTCGT GAACACGCTA CCGGTCTCGA CTGGATCCGA
  121 TGGCCTCCTC CGAGGACGTC ATCAAGGAGT TCATGCGCTT CAAGGTGCGC ATGGAGGGCT
  181 CCGTGAACGG CCACGAGTTC GAGATCGAGG GCGAGGGCGA GGGCCGCCCC TACGAGGGCA
  241 CCCAGACCGC CAAGCTGAAG GTGACCAAGG GCGGCCCCCT GCCCTTCGCC TGGGACATCC
  301 TGTCCCCTCA GTTCCAGTAC GGCTCCAAGG CCTACGTGAA GCACCCCGCC GACATCCCCG
  361 ACTACTTGAA GCTGTCCTTC CCCGAGGGCT TCAAGTGGGA GCGCGTGATG AACTTCGAGG
  421 ACGGCGGCGT GGTGACCGTG ACCCAGGACT CCTCCCTGCA GGACGGCGAG TTCATCTACA
  481 AGGTGAAGCT GCGCGGCACC AACTTCCCCT CCGACGGCCC CGTAATGCAG AAGAAGACCA
  541 TGGGCTGGGA GGCCTCCACC GAGCGGATGT ACCCCGAGGA CGGCGCCCTG AAGGGCGAGA
  601 TCNAGANGAG GCTGAAGCTG AAGGANGGCG GCCACTACGA NGCCNANGTC NAGANCACCT
  661 NCNTGGCCNA GAAGCCCGNG CAGCTGCCNN GCGCCNACNN NANNNNNNNC NNNCNNNNNN
721 TCNNNN
//
Primer: PRE_rev (Pr0250)
Region: PRE,insert 3'
Sequence file: RDB19559_B2C2b.seq check
>06387_19559_B2C2_1_PRE_rev_B05_05_ABI24.ab1
    1 NNNNNNNNNN NNNGNGCAAC ATAGTTAAGA ATACCNGTCA ATCTTTCACA AATTTTGTAA
   61 TCCAGAGGTT GATTATCGAT AAGCTTGATC CCTCGATGTT AACTCTAGAG GATCCGCGGC
  121 CGCGAATTCT TAGGCGCCGG TGGAGTGGCG GCCCTCGGCG CGCTCGTACT GTTCCACGAT
  181 GGTGTAGTCC TCGTTGTGGG AGGTGATGTC CAGCTTGATG TCGGTCTTGT AGGCGCCGGG
  241 CAGCTGCACG GGCTTCTTGG CCATGTAGGT GGTCTTGACC TCGGCGTCGT AGTGGCCGCC
  301 GTCCTTCAGC TTCAGCCTCA TCTTGATCTC GCCCTTCAGG GCGCCGTCCT CGGGGTACAT
  361 CCGCTCGGTG GAGGCCTCCC AGCCCATGGT CTTCTTCTGC ATTACGGGGC CGTCGGAGGG
  421 GAAGTTGGTG CCGCGCAGCT TCACCTTGTA GATGAACTCG CCGTCCTGCA GGGAGGAGTC
  481 CTGGGTCACG GTCACCACGC CGCCGTCCTC GAAGTTCATC ACGCGCTCCC ACTTGAAGCC
  541 CTCGGGGAAG GACAGCTTCN AGTAGTCNGG GANGTCNGCG GGGTGCTTCN CGTANGCCTT
  601 GGAGCCGTAC TGNAACTGAN GGGACNGGAT GTCCCNNGCG AAGGGNANGG GGNCNNCCNN
  661 TNNNNNANNN TNNGNNNNNN GGNCNNNNGN CCTCNNANGG NNNNNCNNNN NCNNNNNCCN
721 TCNNNNNNNN NN
//
Primer: CMV_Forward (Pr0016)
Region: CMV pro,5' LTR,HIV-1 psi
Sequence file: RDB19559_B2C2c.seq check
>06387_19559_B2C2_1_CMV_Forward_C05_08_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN ACGCAAANGN NNGGTAGGCG TGTACGGTGG GAGGTCTATA
   61 TAAGCAGCGC GTTTTGCCTG TACTGGGTCT CTCTGGTTAG ACCAGATCTG AGCCTGGGAG
  121 CTCTCTGGCT AACTAGGGAA CCCACTGCTT AAGCCTCAAT AAAGCTTGCC TTGAGTGCTT
  181 CAAGTAGTGT GTGCCCGTCT GTTGTGTGAC TCTGGTAACT AGAGATCCCT CAGACCCTTT
  241 TAGTCAGTGT GGAAAATCTC TAGCAGTGGC GCCCGAACAG GGACTTGAAA GCGAAAGGGA
  301 AACCAGAGGA GCTCTCTCGA CGCAGGACTC GGCTTGCTGA AGCGCGCACG GCAAGAGGCG
  361 AGGGGCGGCG ACTGGTGAGT ACGCCAAAAA TTTTGACTAG CGGAGGCTAG AAGGAGAGAG
  421 ATGGGTGCGA GAGCGTCAGT ATTAAGCGGG GGAGAATTAG ATCGCGATGG GAAAAAATTC
  481 GGTTAAGGCC AGGGGGAAAG AAAAAATATA AATTAAAACA TATAGTATGG GCAAGCAGGG
  541 AGCTAGAACG ATTCGCAGTT AATCCTGGCC TGTTAGAAAC ATCAGAANGC TGTAGACNAA
  601 TACTGGGACA GCTACNACCN TCCCTTCNGA CNGGNTCAGA ANAANTTANA TCATTATATN
661 ATACNGTAGC NNNCNTCTAN NGNGNGCATC NNNNATAGAN NNNAANANNN NNNNNNN
//
Primer: RRE_check (Pr0837)
Region: RRE
Sequence file: RDB19559_B2C2d.seq check
>06387_19559_B2C2_1_RRE_check_D05_11_ABI24.ab1
    1 NNNNNNNNCT NNNTNNTTCT TTTCTTGCTG GTTTTGCGAT TCTTCAATTA AGGAGTGTAT
   61 TAAGCTTGTG TAATTGTTAA TTTCTCTGTC CCACTCCATC CAGGTCGTGT GATTCCAAAT
  121 CTGTTCCAGA GATTTATTAC TCCAACTAGC ATTCCAAGGC ACAGCAGTGG TGCAAATGAG
  181 TTTTCCAGAG CAACCCCAAA TCCCCAGGAG CTGTTGATCC TTTAGGTATC TTTCCACAGC
  241 CAGGATTCTT GCCTGGAGCT GCTTGATGCC CCAGACTGTG AGTTGCAACA GATGCTGTTG
  301 CGCCTCAATA GCCCTCAGCA AATTGTTCTG CTGCTGCACT ATACCAGACA ATAATTGTCT
  361 GGCCTGTACC GTCAGCGTCA TTGACGCTGC GCCCATAGTG CTTCCTGCTG CTCCCAAGAA
  421 CCCAAGGAAC AAAGCTCCTA TTCCCACTGC TCTTTTTTCT CTCTGCACCA CTCTTCTCTT
  481 TGCCTTGGTG GGTGCTACTC CTAATGGTTC AATTTTTACT ACTTTATATT TATATAATTC
  541 ACTTCTCCAA TTGTCCCTCA TATCTCCTCC TCCAGGTCTG AAGATCAGCG GCCCTTGCTG
  601 TGCGGTGGTC TTACTTTTGT TTTGCTCTTC CTCTATCTTG TCTAAAGCTT CCNTGGTGTC
  661 TTTTATCTCT ATCCNTTGAT GCACACNATA NANGGNTGCT NCTGNANNNT ANNNNGNNCN
721 NANNTNNNNN NNNNN
//
Primer: EF1a-N_R (Pr0024)
Region: EF1 alpha pro,cPPT_CTS
Sequence file: RDB19559_B2C2e.seq check
>06387_19559_B2C2_1_EF1a-N_R_E05_14_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNGCG NNGTGCGCTC TGCCCNCTGA CGGGCACCGG ANCCTCACGA
   61 AGCTTGAATT AATTCAAAGT GAATTTCTGC TGTCCCTGTA ATAAACCCGA AAATTTTGAA
  121 TTTTTGTAAT TTGTTTTTGT AATTCTTTAG TTTGTATGTC TGTTGCTATT ATGTCTACTA
  181 TTCTTTCCCC TGCACTGTAC CCCCCAATCC CCCCTTTTCT TTTAAAATTG TGGATGAATA
  241 CTGCCATTTG TAGATCCGTT CACTAATCGA ATGGATCTGT CTCTGTCTCT CTCTCCACCT
  301 TCTTCTTCTA TTCCTTCGGG CCTGTCGGGT CCCCTCGGGG TTGGGAGGTG GGTCTGAAAC
  361 GATAATGGTG AATATCCCTG CCTAACTCTA TTCACTATAG AAAGTACAGC AAAAACTATT
  421 CTTAAACCTA CCAAGCCTCC TACTATCATT ATGAATAATT TTATATACCA CAGCCAATTT
  481 GTTATGTTAA ACCAATTCCA CAAACTTGCC CATTTATCTA ATTCCAATAA TTCTTGTTCA
  541 TTCTTTTCTT GCTGGTTTTG CGATTCTTCA ATTAAGGAGT GTATTAAGCT TGTGTAATTG
  601 TTAATTTCTC TGTCCCACTN NNTCCAGGTC GTGTGATTCC NAATCTGTTC CNNANNNTTA
  661 TTACTCNNNC TAGNANTNCN NGGCACNNNA NTGGNGCNAA TGANTTTNNC NNANCNNCCN
721 NNANTNNCNN NNNNNNNN
//
Primer: bGH_rev3 (Pr0885)
Region: bGH pA,3' LTR
Sequence file: RDB19559_B2C2f.seq check
>06387_19559_B2C2_1_bGH_rev3_F05_17_ABI24.ab1
    1 NNNNNNNNNN NGGNGGNNGG NNNNNNACAG ATGGCTGGCA ACTAGAAGGC ACAGTCGAGG
   61 CTGATCAGCG GGTTTAAACG GGCCCTGCTA GAGATTTTCC ACACTGACTA AAAGGGTCTG
  121 AGGGATCTCT AGTTACCAGA GTCACACAAC AGACGGGCAC ACACTACTTG AAGCACTCAA
  181 GGCAAGCTTT ATTGAGGCTT AAGCAGTGGG TTCCCTAGTT AGCCAGAGAG CTCCCAGGCT
  241 CAGATCTGGT CTAACCAGAG AGACCCAGTA CAGTCCGGAT GCAGCTCTCG GGCCATGTGA
  301 TGAAATGCTA GGCGGCTGTC AAACCTCCAC TCTAATACTT CTCTCTCCGG GTCATCCATC
  361 CCATGCAGGC TCACAGGGTG TAACAAGCGG GTGTTCTCTC CTTCATTGGC TTCTTCTACC
  421 TTCTCTTGCT CAACTGGTAC TAGCTTGTAG CACCATCCAA AGGTCAGTGG ATATCTGATC
  481 CCTGGCCCTG GTGTGTAGTT CTGCCAATCA GGGAAGTAGC CTTGTGTGTG GTAGATCCAC
  541 AGATCAAGGA NATCTTGTCT TCGTTGGGAG TGAATTAGCC CTTCCAGTCC CCCCTTTTCT
  601 TTTAAAAAGT GGCTAAGANC TACNGCTGCC TTGNAAGTCN TTGGTCNTAA ANGTACNNGA
  661 NGTGTGNNTN GNAAAANCNN NCTCCNNNNN NNNGTGCNNN NANNNNNNCN CNNNNNNNNN
721 N
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40 pro_ori,Zeo
Sequence file: RDB19559_B2C2g.seq check
>06387_19559_B2C2_1_SV40pro_ori_F_G05_20_ABI24.ab1
    1 NNNNNNNNNN NNNCGCCTCT GCCTCTGANC TATTCCNGAA GTAGTGAGGA GGCTTTTTTG
   61 GAGGCCTAGG CTTTTGCAAA AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC
  121 AGCACGTGTT GACAATTAAT CATCGGCATA GTATATCGGC ATAGTATAAT ACGACAAGGT
  181 GAGGAACTAA ACCATGGCCA AGTTGACCAG TGCCGTTCCG GTGCTCACCG CGCGCGACGT
  241 CGCCGGAGCG GTCGAGTTCT GGACCGACCG GCTCGGGTTC TCCCGGGACT TCGTGGAGGA
  301 CGACTTCGCC GGTGTGGTCC GGGACGACGT GACCCTGTTC ATCAGCGCGG TCCAGGACCA
  361 GGTGGTGCCG GACAACACCC TGGCCTGGGT GTGGGTGCGC GGCCTGGACG AGCTGTACGC
  421 CGAGTGGTCG GAGGTCGTGT CCACGAACTT CCGGGACGCC TCCGGGCCGG CCATGACCGA
  481 GATCGGCGAG CAGCCGTGGG GGCGGGAGTT CGCCCTGCGC GACCCGGCCG GCAACTGCGT
  541 GCACTTCGTG GCCGAGGANC ANGACTGACA CGTGCTACGA GATTTCNATT CCNCCGCCNC
  601 CTTCTATGNN GGNTGGGCTT CNGAATCNNT TTCCGGGACN CCGGCTGGNT GATCCTCNNN
  661 CGCGGGGNNC TCATGCTGGA NTNNNNCGCC CNNCCCCNNN NTGNTTNNNG NNGCNTNNNN
721 NNGNNTNNNN ANNAANNNNN NNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

user_report Matsumoto, R., AP-1B regulates interactions of epithelial cells and intraepithelial lymphocytes in the intestine. Cell. Mol. Life Sci. 81 (1): 425 (2024). PMID 39369131. [link to RRC of NBRP]
user_report Badiee, P., Intratumoral Anti-PD-1 Nanoformulation Improves Its Biodistribution. ACS Appl. Mater. Interfaces 14 (14): 15881-15893 (2022). PMID 35357803. [link to RRC of NBRP]
user_report Yoshida, S., Generation of stratified squamous epithelial progenitor cells from mouse induced pluripotent stem cells. PLoS One, 6 (12): e28856 (2012). PMID 22174914. [link to RRC of NBRP]