Please review the QC test results indicated by icon below as well as clone information before placing your order.

pCMFlag_hsMAFG (#RDB06413)

Expression vector of human MAFG.

Alternative name	SET-0065_6
Clone info.	Expression vector of human MAFG. Expression was confirmed by Western blotting with anti-FLAG antibody.
Comment	Derived from Sugano BC012327. PCR cloning, forward primer: ATGACGACCCCCAATAAAGG; reverse primer: CTACGATCGGGCATCCGTCT.
Vector backbone	pCMV_S-FLAG (RDB05956) (Plasmid)
Size of vector backbone	5.5 kb
Selectable markers	Amp^r (E. coli), Neo^r (mammalian cell)
Growth conditions	37C, LB+Amp
Gene/insert name	Human MAFG cDNA
Depositor|Developer	DNA Bank, |

 

Remarks, protocol and/or map (pdf)	RDB06413.pdf

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] MTA, for use for for-profit purpose [Word] Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution

The RECIPIENT agrees to describe RIKEN BRC as the source of the MATERIAL and cite Gene 200, 149-156, 1997 in any publication. Additional terms and conditions: Regrding pRc/CMV: a) The modified pRc/CMV, and their descendants (collectively, LIFE MATERIALS), should be used for pre-clinical/non-human, research use only. b) LIFE MATERIALS shall not be used for any commercial purposes. c) LIFE MATERIALS shall not be transferred beyond the specified recipient. d) Recipient must contact Thermo Fisher Scientific Inc. at outlicense@thermofisher.com for any desired use of LIFE MATERIALS beyond the scope of a) to c) above.

提供案内 (日本国内) [open/close]

Catalog #	Resource name	Shipping form	Fee
RDB06413	pCMFlag_hsMAFG	DNA solution	JPY 9,460 (not-for-profit academic purpose) JPY 18,920 (for-profit-research purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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How to cite this biological resource

Materials & Methods section:

The pCMFlag_hsMAFG was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06413).

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB13708_A5Kap1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward Sequence file: RDB13708_A5Kaa.seq
>06413_13708_A5Ka_CMV-Forward_A05_02.ab1     1 ATTAACTCGC CCATTGACGC AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC    61 AGAGCTCTCT GGCTAACTAG AGAACCCACT GCTTACTGGC TTATCGAAAT TAATACGACT   121 CACTATAGGG AGACCCAAGC TGAATTCACC ATGGACTACA AGGACGATGA CGATAAGGCG   181 GCCATGACGA CCCCCAATAA AGGAAACAAG GCCTTGAAGG TGAAGCGGGA GCCGGGTGAG   241 AATGGCACCA GCCTGACGGA TGAGGAGCTG GTGACCATGT CGGTGCGGGA GCTGAACCAG   301 CACCTGCGGG GCCTGTCCAA GGAGGAGATC GTCCAGCTGA AGCAGCGCCG GCGCACGCTC   361 AAGAACCGCG GCTACGCTGC CAGCTGCCGC GTGAAGCGGG TGACGCAGAA GGAGGAGCTG   421 GAGAAGCAGA AGGCGGAGCT GCAGCAGGAG GTGGAGAAGC TGGCCTCAGA GAACGCCAGC   481 ATGAAGCTGG AGCTCGACGC GCTGCGCTCC AAGTACGAGG CGCTGCAGAC CTTCGCCCGG   541 ACGGTGGCCC GCAGCCCCGT GGCGCCAGCC CGGGGCCCCC TTGCCGCCGG CCTGGGGCCC   601 CTCGTCCCAG GCAAGGTGGC CGCCACCAGC GTCATCACAA TAGTAAAGTC CAAGACGGAT   661 GCCCGATCGT AGGGCCGCTC GAGCATGCAT CTAGAGGGCC CTATTCTATA GTGTCACCTA   721 AATGCTAGAG CTCGCTGATC AGCCTCGACT GTGCCTTCTA GTTGCCAGCC ATCTGTTGTT   781 TGCCCCTCCC CCGTGCCTTC CTTGACCCTG GAAGGTGCCA CTCCCACTGT CCTTTCCTAA   841 TAAAATGAGG AAATTGCATC GCATTGTCTG AGTATGTGTC ATTCTATTCT GGGGTGTGGT   901 TTAGCCAGGA CAGCAAGGGG GGGGAGGGAT TGGGAAGACC ATAGCAGGGC ATGCTGGGGA   961 ATGCGTGGGC TCTATGGCTT CCTGAGGCGG AAGAACCTAG CTGGAGCTCG AAGGGGAATC 1021 GCCACGTCGC CTGTAGCGGC CGCATT //
Primer: SV40pro_F_V2 Sequence file: RDB13708_A5Kab.seq
>06413_13708_A5Ka_SV40pro_F_V2_B05_05.ab1     1 GGGGACGTTT TGGAGCTAGG CTTTTGCAAA AGCTCCCGGG AGCTTGGATA TCCATTTTCG    61 GATCTGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT GGATTGCACG   121 CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA   181 TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG   241 TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG CGGCTATCGT   301 GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA   361 GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC   421 CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG   481 CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG   541 AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG   601 AACTGTTCGC CAGGCTCAAG GCGCGCATGC CCGACGGCGA GGATCTCGTC GTGACCCATG   661 GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT   721 GTGGCCGGCT GGGTGTGGCG GACCGCTATC AGGACATAGC GTTGGCTACC CGTGATATTG   781 CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCGT GCTTTACGGT ATCGCCGCTC   841 CCGATTCGCA GCGCATCGCC TTCTATCGCC TTCTTGACGA GTTCTTCTGA GCGGGACTCT   901 GGGGGTTCGA AATGACCGAC CAAGCGACGC CCAACCTGCC ATCACGAGAT TTCGATTCCA   961 CCGCCGCCCT TCTATGAAGG GTTTGGGGCT TCGGAATCGT TTTCCCGGGG ACGCCCGGCT  1021 GGATTGATTC CTTCCAGCGG CGGGGATCTC AATGCTGGGA AGTCCATCGG CCCAACCCCA 1081 ACTTGTTTTA TTTGCCAGGC GCCAG //

Please visit Sequencing and PCR primers for primer information.

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References

Original, user report and related articles

Resource search

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Link

Other resources in our bank

• human MAFG

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External Database
Human MAFG

• in KEGG Gene
• in NCBI Gene

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Reference sequence
  

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