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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pARG1K-FFH1c

S. pombe 3 ter end FLAG2-His6-tagged vector with nmt1 promoter

Catalog number RDB06437
Resource name pARG1K-FFH1c
Clone info. S. pombe 3 ter end FLAG2-His6-tagged vector with nmt1 promoter.
Comment Gateway compatible.
Vector backbone pUC119 (Plasmid)
Selectable markers E. coli: Amp^r, Cm^r, ccdB.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Gene/insert name ccdB
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
Other clones in our bank
External Database
ccdB
            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Nat. Biotechnol., 24, 841-847 (2006)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
            Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Remarks Use Survival2 (Invitrogen) or equivalent to amplify this clone.
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Nat. Biotechnol., 24, 841-847 (2006)) の引用と、寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            備考 このクローンの増殖にはSurvival2 (Invitrogen) あるいは同等の宿主を使用してください。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB06437 pARG1K-FFH1c DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB06437z.seq

            Original reference

            original Matsuyama, A., ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe. Nat. Biotechnol., 24, 841-847 (2006). PMID 16823372.
            original Matsuyama, A., ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe. Nat. Biotechnol., 24, 841-847 (2006). PMID 16823372.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB12268_A3J7.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer:
            Sequence file: RDB12268_A3J7a.seq check
            >06437_12268_A3J7_nmt1Fw_G07_19.ab1
                1 AGGAAATTTA TTAATCAGGA AACGTAACTC TCGGCTACTG GATGGTTCAG TCACCCAACG
               61 ATTACTGGGG AGAGAAAACA GGGCAAAAGC AAAGCTTAAA GGAATCCGAT TGTCATTCGG
              121 CAATGTGCAG CGAAACTAAA AACCGGATAA TGGACCTGTT AATCGAAACA TTGAAGATAT
              181 ATAAAGGAAG AGGAATCCTG GCATATCATC AATTGAATAA GTTGAATTAA TTATTTCAAT
              241 CTCATTCTCA CTTTCTGACT TATAGTCGCT TTGTTAAAGC TAGCGTCGAC AGGCCTGGAT
              301 CCGGATATCA ACAAGTTTGT ACAAAAAAGC TGAACGAGAA ACGTAAAATG ATATAAATAT
              361 CAATATATTA AATTAGATTT TGCATAAAAA ACAGACTACA TAATACTGTA AAACACAACA
              421 TATCCAGTCA CTATGGCGGC CGCATTAGGC ACCCCAGGCT TTACACTTTA TGCTTCCGGC
              481 TCGTATAATG TGTGGATTTT GAGTTAGGAT CCGTCGAGAT TTTCAGGAGC TAAGGAAGCT
              541 AAAATGGAGA AAAAAATCAC TGGATATACC ACCGTTGATA TATCCCAATG GCATCGTAAA
              601 GAACATTTTG AGGCATTTCA GTCAGTTGCT CAATGTACCT ATAACCAGAC CGTTCAGCTG
            661 GATATTACGG CC
            //
            Primer:
            Sequence file: RDB12268_A3J7b.seq check
            >06437_12268_A3J7_ADHrev_H07_22.ab1
                1 GGGAGAGGTA ATCAAGGAAT AAGATTTTCG TTTTAAAACC TAAGAGTCAC TTTAAAATTT
               61 GTATACACTT ATTTTTTTTA TAACTTATTT AATAATAAAA ATCATAAATC ATAAGAAATT
              121 CGCTTATTTA GAAGTGGCGC GCCGGATCCT CTAGAGTCGA GATCTCAGTG GTGATGGTGG
              181 TGATGTTTGT CATCGTCGTC CTTGTAGTCC ATGGGTTTAT CGTCATCATC CTTATAATCC
              241 ATGATATCAA CCACTTTGTA CAAGAAAGCT GAACGAGAAA CGTAAAATGA TATAAATATC
              301 AATATATTAA ATTAGATTTT GCATAAAAAA CAGACTACAT AATACTGTAA AACACAACAT
              361 ATCCAGTCAC TATGGTCGAC CTGCAGACTG GCTGTGTATA AGGGAGCCTG ACATTTATAT
              421 TCCCCAGAAC ATCAGGTTAA TGGCGTTTTT GATGTCATTT TCGCGGTGGC TGAGATCAGC
              481 CACTTCTTCC CCGATAACGG AGACCGGCAC ACTGGCCATA TCGGTGGTCA TCATGCGCCA
              541 GCTTTCATCC CCGATATGCA CCACCGGGTA AAGTTCACGG GAGACTTTAT CTGACAGCAG
              601 ACGTGCACTG GCCAGGGGGA TCACCATCCG TCGCCCGGGC GTGTCAATAA TATCACTCTG
            661 TACATCCACA AACAGA
            //
            Primer:
            Sequence file: RDB12268_A3J7c.seq check
            >06437_12268_A3J7_nmt1Fw2_A08_02.ab1
                1 GCCTTGGAGA CGATGACAAC ATCACCACCA TCACCACTGA GATCTCGACT CTAGAGGATC
               61 CGGCGCGCCA CTTCTAAATA AGCGAATTTC TTATGATTTA TGATTTTTAT TATTAAATAA
              121 GTTATAAAAA AAATAAGTGT ATACAAATTT TAAAGTGACT CTTAGGTTTT AAAACGAAAA
              181 TTCTTATTCT TGAGTAACTC TTTCCTGTAG GTCAGGTTGC TTTCTCAGGT ATAGTATGAG
              241 GTCGCTCTTA TTGACCACAC CTCTACCGGC AGATCCGCTA GGGATAACAG GGTAATATAC
              301 TAGTCGTAGC TGCTAAAGGT GAGGGCTCCT ATTTGTTTGA CAAGGAAGGT CGTAAATACA
              361 TTGACTTCAC CTCTGGCGTA GCTGTTACAT CTTTGGGTCA TGCTCATCCT GAAGTTGCTA
              421 GACTTGCCGC TGATCAATGC TCCAAATTAG TCCATAGTAG CAACCTTTTC TACAACGAGC
              481 CTGCCATTGA GCTATCTAAT GTCATTAATA ATTCTTTGGC AAAAAATAGC GGTATTGCTG
              541 GCCCCACAAA AATTTTCTTT GCCAATTGTG GTACTGAGGC CAATGAGACA GCCTTGAAGT
              601 TCGCTCGTAA GGCGGCCGCG GGCCCGCGGC CGCGTTTGAG AAGTATGGAG AAGGGAAGAG
            661 TCAAATTGTT TACTT
            //

            Please visit Sequencing and PCR primers for primer information.

            References

            Original, user report and related articles

            original Matsuyama, A., ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe. Nat. Biotechnol., 24, 841-847 (2006). PMID 16823372.

            2022.05.18

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