RDB06446 - pHIS3K-CFH21c

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

pHIS3K-CFH21c (#RDB06446)

S. pombe 3 ter end CFP-FLAG-His6-tagged vector with tub1 promoter

Clone info.	S. pombe 3 ter end CFP-FLAG-His6-tagged vector with tub1 promoter.
Comment	Gateway compatible.
Vector backbone	pUC119 (Plasmid)
Selectable markers	E. coli: Amp^r, Cm^r, ccdB.
Growth remarks	Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor\|Developer	Yoshida, Minoru \| Matsuyama, Akihisa \|

Sequence	RDB06446z.seq

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature(s). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.

Remarks Use Survival2 (Invitrogen) or equivalent to amplify this clone.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature(s). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
Remarks	Use Survival2 (Invitrogen) or equivalent to amplify this clone.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献の引用と、寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
備考	このクローンの増殖にはSurvival2 (Invitrogen) あるいは同等の宿主を使用してください。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB06446	pHIS3K-CFH21c	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pHIS3K-CFH21c was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06446).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB17486_A9I5p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: Cm_M_R (Pr0012) Region: CmR,lac UV5pro,attR1 Sequence file: RDB17486_A9I5a.seq
>06446_17486_A9I5_3_Cm-M_R_G10_19_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NTNNCCNGCT NNCCGTCTTT NNTTGCCNTA CGGAATTCCG 61 GATGAGCATT CATCNNGCGG GCAAGAATGT GAATAAAGGC CGGATAAAAC TTGTGCTTAT 121 TTTTCTTTAC GGTCTTTAAA AAGGCCGTAA TATCCAGCTG AACGGTCTGG TTATAGGTAC 181 ATTGAGCAAC TGACTGAAAT GCCTCAAAAT GTTCTTTACG ATGCCATTGG GATATATCAA 241 CGGTGGTATA TCCAGTGATT TTTTTCTCCA TTTTAGCTTC CTTAGCTCCT GAAAATCTCG 301 ACGGATCCTA ACTCAAAATC CACACATTAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG 361 GGGTGCCTAA TGCGGCCGCC ATAGTGACTG GATATGTTGT GTTTTACAGT ATTATGTAGT 421 CTGTTTTTTA TGCAAAATCT AATTTAATAT ATTGATATTT ATATCATTTT ACGTTTCTCG 481 TTCAGCTTTT TTGTACAAAC TTGTTGATAT CCGGATCCAG GCCTGTCGAC GCTAGCGAAC 541 GTCTTTCCAG GGTTTCTACG AGGTTTTTGG TGTTTGGTTT GTAGTGAAGT GCGGTGTACA 601 CTTAATCAAA ACAATTCCTT AATAAAGAAG TCTAACGATA TTTATTGATG ATACGGAAGG 661 AAGTATGCTA AACATGTAAA TAGACATTAA AATAATGCAT TGCTTGCAAA AAGAAGCTTA 721 CTCAGTCAGG TGTTTTAAGC TTTCTAATCA TAAAAAGATT ANGATCTTCA TCAATTTCAC 781 CTTTTATTTT CTCAATTTGT AAGTGAAAGA TTGGATTGGT AAATTTCCAT TAAAACTCAA 841 CATGTGCTCC TTNATAGAAC AGGTATATCC TCATGTAAAT CTTTTTAAAA GTATATAAAA 901 TATTACATN //
Primer: ccdB-C_F (Pr0136) Region: ccdB,attR2,ECFP Sequence file: RDB17486_A9I5b.seq
>06446_17486_A9I5_3_ccdB-C_F_A11_02_ABI24.ab1 1 NNNNNNNNNN NNNNNNTCNN GGNNGNANGN TGGCGCATGA TGACCACCGA TATGGCCAGT 61 GTGCCGGTCT CCGTTATCGG GGAAGAAGTG GCTGATCTCA GCCACCGCGA AAATGACATC 121 AAAAACGCCA TTAACCTGAT GTTCTGGGGA ATATAAATGT CAGGCTCCCT TATACACAGC 181 CAGTCTGCAG GTCGACCATA GTGACTGGAT ATGTTGTGTT TTACAGTATT ATGTAGTCTG 241 TTTTTTATGC AAAATCTAAT TTAATATATT GATATTTATA TCATTTTACG TTTCTCGTTC 301 AGCTTTCTTG TACAAAGTGG TTGATATCAT GGTGAGCAAG GGCGAGGAGC TGTTCACCGG 361 GGTGGTGCCC ATCCTGGTCG AGCTGGACGG CGACGTAAAC GGCCACAAGT TCAGCGTGTC 421 CGGCGAGGGC GAGGGCGATG CCACCTACGG CAAGCTGACC CTGAAGTTCA TCTGCACCAC 481 CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC CTGACCTGGG GCGTGCAGTG 541 CTTCAGCCGC TACCCCGACC ACATGAAGCA GCACGACTTC TTCAAGTCCG CCATGCCCGA 601 AGGCTACGTC CAGGAGCGCA CCATCTTCTT CAAGGACGAC GGCAACTACA AGACCCGCGC 661 CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC GAGCTGAAGG GCATCGACTT 721 CAAGGANGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC AACTACATCA GCCACNACGT 781 CTATATCACC GCCNACAAGC AGAAGAACGG CATCAAGGCC AACTTCAAGA TCCGCCACAA 841 CATCGANGAN GG //
Primer: EGFP-N_F (Pr0649) Region: ECFP,FLAG,6xHis,ADH1ter Sequence file: RDB17486_A9I5c.seq
>06446_17486_A9I5_3_EGFP-N_F_B11_05_ABI24.ab1 1 NNNNNNNNNN NNNGGNNNNG GNGNNGGCGN NGCNNCCTAC GGNAAGCTGN NCCTGAAGTT 61 CATCTGCACC ACCGGNAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA CCCTGACCTG 121 GGGCGTGCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT TCTTCAAGTC 181 CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG ACGGCAACTA 241 CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA TCGAGCTGAA 301 GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT ACAACTACAT 361 CAGCCACAAC GTCTATATCA CCGCCGACAA GCAGAAGAAC GGCATCAAGG CCAACTTCAA 421 GATCCGCCAC AACATCGAGG ACGGCAGCGT GCAGCTCGCC GACCACTACC AGCAGAACAC 481 CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGAGCA CCCAGTCCGC 541 CCTGAGCAAA GACCCCAACG AGAAGCGCGA TCACATGGTC CTGCTGGAGT TCGTGACCGC 601 CGCCGGGATC ACTCTCGGCA TGGACGAGCT GTACAAGCCC ATGGACTACA AGGACGACGA 661 TGACAAACAT CACCACCATC ACCACTGAGA TCTCGACTCT AGAGGATCCG GCGCGCCACT 721 TCTAAATAAG CGAATTTCTT ATGANTTATG ATTTTTATTA TTAAATAAGT TATAAAAAAA 781 ATAAGTGTAT ACAAATTTTA AAGTGACTCT TANGTTTTAA AACGAAAATT CTTATTCTTG 841 ANTAACTCTT TCCTGTAGGT CANGN //
Primer: Venus-C_F (Pr0452) Region: FLAG,6xHis,ADH1ter Sequence file: RDB17486_A9I5d.seq
>06446_17486_A9I5_3_Venus-C_F2_H10_22_ABI24.ab1 1 NNNNNNNNNN NNNNNNGNNN NNNTCTCGGN ATGGNNGAGC TGTACNAGCC CNTGGNCTAC 61 AAGGACGACG ATGACAAACA TCACCACCAT CACCACTGAG ATCTCGACTC TAGAGGATCC 121 GGCGCGCCAC TTCTAAATAA GCGAATTTCT TATGATTTAT GATTTTTATT ATTAAATAAG 181 TTATAAAAAA AATAAGTGTA TACAAATTTT AAAGTGACTC TTAGGTTTTA AAACGAAAAT 241 TCTTATTCTT GAGTAACTCT TTCCTGTAGG TCAGGTTGCT TTCTCAGGTA TAGTATGAGG 301 TCGCTCTTAT TGACCACACC TCTACCGGCA GATCCGCTAG GGATAACAGG GTAATATACT 361 AGTGTTGGCA GCGATGAAAT TATTGACTCC TTAATTCGCA TTTCTTGTAT TCCTGGAAAA 421 GATAAGATTT TGATGTGTCC ACCTTCGTAC GGAATGTATA CGGTCTCTGC AAAAATCAAC 481 GATGTTGAGG TTGTTAAGGT TCTCCTTGAA CCAGATTTCA ATTTGAACGT CGATGCTATT 541 TGCGAAACAC TTTCTAAAGA TAGTGCCATC AAAGTCTTTT TTGCTTGTTC CCCTGGTAAT 601 CCAACAGCGG CCGCGGGCCC GCGGCCGCTC TTAAACTCGA GGATATTAAG AAGATTTTGG 661 AGCACCCTAC ATGGAATGGA ATCGTTGTCG TCGATGAGGC CTATATTGAT TTCTCTGCTC 721 CCGATATGTC TGCCTTAACT CTTGTCAATG AATATCCNAA CCT //
Primer: TEFpro_F (Pr0372) Region: KanMX Sequence file: RDB17486_A9I5e.seq
>06446_17486_A9I5_3_TEFpro_F_D11_11_ABI24.ab1 1 NNNNNNNNNN NNNNNNAANN NNNNGGCTCC TCGCTGCAGA NCTGCGAGCA GGGAANCGCT 61 CCCCTCACAG ACGCGTTGAA TTGTCCCCAC GCCGCGCCCC TGTAGAGAAA TATAAAAGGT 121 TAGGATTTGC CACTGAGGTT CTTCTTTCAT ATACTTCCTT TTAAAATCTT GCTAGGATAC 181 AGTTCTCACA TCACATCCGA ACATAAACAA CCATGGGTAA GGAAAAGACT CACGTTTCGA 241 GGCCGCGATT AAATTCCAAC ATGGATGCTG ATTTATATGG GTATAAATGG GCTCGCGATA 301 ATGTCGGGCA ATCAGGTGCG ACAATCTATC GATTGTATGG GAAGCCCGAT GCGCCAGAGT 361 TGTTTCTGAA ACATGGCAAA GGTAGCGTTG CCAATGATGT TACAGATGAG ATGGTCAGAC 421 TAAACTGGCT GACGGAATTT ATGCCTCTTC CGACCATCAA GCATTTTATC CGTACTCCTG 481 ATGATGCATG GTTACTCACC ACTGCGATCC CCGGCAAAAC AGCATTCCAG GTATTAGAAG 541 AATATCCTGA TTCAGGTGAA AATATTGTTG ATGCGCTGGC AGTGTTCCTG CGCCGGTTGC 601 ATTCGATTCC TGTTTGTAAT TGTCCTTTTA ACAGCGATCG CGTATTTCGT CTCGCTCAGG 661 CGCAATCACG AATGAATAAC GGTTTGGTTG ATGCGAGTGA TTTTGATGAC GAGCGTAATG 721 GCTGGCCTGT TGAACAAGTC TGGAAAGAAA TGCATAAGCT TTTGCCATTC TCACCGGATT 781 CAGTCGTCAC TCATGGTGAT TTCTCACTTG ATAACCTTAT TTTTGACGAG GGGAAATTAA 841 TAGGTTGTAT TGATGTTGGN //
Primer: M13fwd (Pr0349) Region: KanMX Sequence file: RDB17486_A9I5f.seq
>06446_17486_A9I5_3_M13fwd_E11_14_ABI24.ab1 1 NNNNNNNNNN NNNNNNNGNG GNGTTNGTNN CGAATCGACN GCAGTATAGC GACCAGCATT 61 CACATACGAT TGACGCATGA TATTACTTTC TGCGCACTTA ACTTCGCATC TGGGCAGATG 121 ATGTCGAGGC GAAAAAAAAT ATAAATCACG CTAACATTTG ATTAAAATAG AACAACTACA 181 ATATAAAAAA ACTATACAAA TGACAAGTTC TTGAAAACAA GAATCTTTTT ATTGTCAGTA 241 CTGATTAGAA AAACTCATCG AGCATCAAAT GAAACTGCAA TTTATTCATA TCAGGATTAT 301 CAATACCATA TTTTTGAAAA AGCCGTTTCT GTAATGAAGG AGAAAACTCA CCGAGGCAGT 361 TCCATAGGAT GGCAAGATCC TGGTATCGGT CTGCGATTCC GACTCGTCCA ACATCAATAC 421 AACCTATTAA TTTCCCCTCG TCAAAAATAA GGTTATCAAG TGAGAAATCA CCATGAGTGA 481 CGACTGAATC CGGTGAGAAT GGCAAAAGCT TATGCATTTC TTTCCAGACT TGTTCAACAG 541 GCCAGCCATT ACGCTCGTCA TCAAAATCAC TCGCATCAAC CAAACCGTTA TTCATTCGTG 601 ATTGCGCCTG AGCGAGACGA AATACGCGAT CGCTGTTAAA AGGACAATTA CAAACAGGAA 661 TCGAATGCAA CCGGCGCAGG AACACTGCCA GCGCATCAAC AATATTTTCA CCTGAATCAG 721 GATATTCTTC TAATACCTGG AATGCTGTTT TGCCGGGGAT CGCAGTGGTG AGTAACCATG 781 CATCATCAGG AGTACGGATA AAATGCTTGA TGGTCGGAAG ANGCATAAAT TCCGTCAGCC 841 AGTTTAGTCT GACCATCTCA TCTGTAACAT CATTGGCAAC GCTACNTT //