Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pLYS1U-HFY21c

S. pombe 5 ter end His6-FLAG-YFP-tagged vector with tub1 promoter

Catalog number RDB06475
Resource name pLYS1U-HFY21c
Clone info. S. pombe 5 ter end His6-FLAG-YFP-tagged vector with tub1 promoter.
Comment Gateway compatible.
Vector backbone pUC119 (Plasmid)
Selectable markers E. coli: Amp^r, Cm^r, ccdB.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
Other clones in our bank

External Database

            Reference sequence
              
             
            Sequence RDB06475z.seq

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature(s). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
            Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
            Order form 
            [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Remarks Use Survival2 (Invitrogen) or equivalent to amplify this clone.
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献の引用と、寄託者への謝辞の表明を必要とする。 利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。 寄託者から要請がある場合、理研BRCは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            備考 このクローンの増殖にはSurvival2 (Invitrogen) あるいは同等の宿主を使用してください。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB06475 pLYS1U-HFY21c DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The pLYS1U-HFY21c was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06475).

            Reference section:

            Further references such as user reports and related articles (go to bottom)


            References and tips

            Featured content

            Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB12323_A3Kl.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer:
            Sequence file: RDB12323_A3Kla.seq check
            >RDB12323_EGFP_C_D11_11.ab1
                1 CGGGGGAATC TCGGCATGCA CGAGCTGTAC AAGGATATCA CAAGTTTGTA CAAAAAAGCT
               61 GAACGAGAAA CGTAAAATGA TATAAATATC AATATATTAA ATTAGATTTT GCATAAAAAA
              121 CAGACTACAT AATACTGTAA AACACAACAT ATCCAGTCAC TATGGCGGCC GCATTAGGCA
              181 CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATAATGT GTGGATTTTG AGTTAGGATC
              241 CGTCGAGATT TTCAGGAGCT AAGGAAGCTA AAATGGAGAA AAAAATCACT GGATATACCA
              301 CCGTTGATAT ATCCCAATGG CATCGTAAAG AACATTTTGA GGCATTTCAG TCAGTTGCTC
              361 AATGTACCTA TAACCAGACC GTTCAGCTGG ATATTACGGC CTTTTTAAAG ACCGTAAAGA
              421 AAAATAAGCA CAAGTTTTAT CCGGCCTTTA TTCACATTCT TGCCCGCCTG ATGAATGCTC
              481 ATCCGGAATT CCGTATGGCA ATGAAAGACG GTGAGCTGGT GATATGGGAT AGTGTTCACC
              541 CTTGTTACAC CGTTTTCCAT GAGCAAACTG AAACGTTTTC ATCGCTCTGG AGTGAATACC
              601 ACGACGATTT CCGGCAGTTT CTACACATAT ATTCGCAAGA TGTGGCGTGT TACGGTGAAA
            661 ACCTGGCCTA TTTCCC
            //
            Primer:
            Sequence file: RDB12323_A3Klb.seq check
            >RDB12323_ADH_rev_E11_14.ab1
                1 TGAGGAAACT CAAGGAATAA GAATTTTCGT TTTAAAACCT AAGAGTCACT TTAAAATTTG
               61 TATACACTTA TTTTTTTTAT AACTTATTTA ATAATAAAAA TCATAAATCA TAAGAAATTC
              121 GCTTATTTAG AAGTGGCGCG CCGGATCCTC TAGAGTCGAG ATCTCATGAT ATCACCACTT
              181 TGTACAAGAA AGCTGAACGA GAAACGTAAA ATGATATAAA TATCAATATA TTAAATTAGA
              241 TTTTGCATAA AAAACAGACT ACATAATACT GTAAAACACA ACATATCCAG TCACTATGGT
              301 CGACCTGCAG ACTGGCTGTG TATAAGGGAG CCTGACATTT ATATTCCCCA GAACATCAGG
              361 TTAATGGCGT TTTTGATGTC ATTTTCGCGG TGGCTGAGAT CAGCCACTTC TTCCCCGATA
              421 ACGGAGACCG GCACACTGGC CATATCGGTG GTCATCATGC GCCAGCTTTC ATCCCCGATA
              481 TGCACCACCG GGTAAAGTTC ACGGGAGACT TTATCTGACA GCAGACGTGC ACTGGCCAGG
              541 GGGATCACCA TCCGTCGCCC GGGCGTGTCA ATAATATCAC TCTGTACATC CACAAACAGA
              601 CGATAACGGC TCTCTCTTTT ATAGGTGTAA ACCTTAAACT GCATTTCACC AGCCCCTGTT
            661 CTCGTCAGCA AAAGAGC
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            user_report Ito, D., Pericentrin-mediated SAS-6 recruitment promotes centriole assembly. Elife 8. pii: e41418 (2019). PMID 31182187.

            2023.05.02

            GNP_filter3_RDBDEP_html_230501.pl