Please review the QC test results indicated by icon below as well as clone information before placing your order.

pDUAL-FFH31c

S. pombe 3 ter end FLAG2-His6-tagged vector with cam1 promoter

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., A., Yeast, 25 (5): 371-376 (2008)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Remarks	Use Survival2 (Invitrogen) or equivalent to amplify this clone.

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast, 25 (5): 371-376 (2008)) の引用と、寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
備考	このクローンの増殖にはSurvival2 (Invitrogen) あるいは同等の宿主を使用してください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB12778_A4Dp.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: ADHrev Sequence file: RDB12778_A4Dpa.seq
>06490_12778_A4Dp_ADHrev_F08_17.ab1     1 GCGGGCCAAG TAAAAATTTC TCAAGAAAAA AGAATTTTCG TTTTAAAACC TAAGACCCCA    61 CTTTAAAATT TGTATACACT TATTTTTTTT ATAACTTATT TAATAATAAA AATCATAAAT   121 CATAAGAAAT TCGCTTATTT AGAAGTGGCG CGCCGGATCC TCTAGAGTCG AGATCTCAGT   181 GGTGATGGTG GTGATGTTTG TCATCGTCGT CCTTGTAGTC CATGGGTTTA TCGTCATCAT   241 CCTTATAATC CATGATATCA ACCACTTTGT ACAAGAAAGC TGAACGAGAA ACGTAAAATG   301 ATATAAATAT CAATATATTA AATTAGATTT TGCATAAAAA ACAGACTACA TAATACTGTA   361 AAACACAACA TATCCAGTCA CTATGGTCGA CCTGCAGACT GGCTGTGTAT AAGGGAGCCT   421 GACATTTATA TTCCCCAGAA CATCAGGTTA ATGGCGTTTT TGATGTCATT TTCGCGGTGG   481 CTGAGATCAG CCACTTCTTC CCCGATAACG GAGACCGGCA CACTGGCCAT ATCGGTGGTC   541 ATCATGCGCC AGCTTTCATC CCCGATATGC ACCACCGGGT AAAGTTCACG GGAGACTTTA   601 TCTGACAGCA GACGTGCACT GGCCAGGGGG ATCACCATCC GTCGCCCGGG CGTGTCAATA   661 ATATCACTCT GTACATCCAC AAACAGACGA TAACGGCTCT CTCTTTTATA GGTGTAAACC   721 TTAAACTGCA TTTCACCCAG CCCCTGTTCT CGTCAGCAAA AGAGCCGTTC ATTTCAAATA   781 AAACCGGGCG ACCTCAGCCA TCCCTTCCTG ATTTTCCGCT TTCCCAGCGT TCCGGCACGC   841 AGACCACGGG CTTCATTCTG CATGGTTGTG CTTACCCAGA CCGGAGATAA TTGACATCAT   901 ATATGCCTTG AGCAAACTGA TAGCTGTCGC TGTCAACTGT CCCTGTAATA CGCCTGCTTC   961 ATAACATACC TCTTTTTGAC ATACTTCGGG GTATACATAT CAGTATAATA TTCTTATACC  1021 GCAAAAAATC AGCGCGCAAA TACCGCATAC TGCTATCTGG CCTTTTAGTA AGCCCGATTC  1081 CAGAATTCTT ACGCCCCCGC CCTGGCCACA CTCAATCCGC CGATACTGGT TTGTAAATTC  1141 AATTAAGGCA ATTCAGCTCG ACACATGGAG GCCATCCACA CCCGCCAGGT GAATGAAACC  1201 CTGAAAATCT GCCTCAAGGC GGCGACTATC TCCCAGAGCA CACCACTCTG TGCTGTCTGC //
Primer: Cm_N_rev Sequence file: RDB12778_A4Dpb.seq
>06490_12778_A4Dp_Cm_N_rev_G08_20.ab1     1 CGTAAACCCC TAAATGTTTC TTTACGATGC CATTGGGATA TATCAACGCG TGGTATATCC    61 AGTGATTTTT TTCTCCATTT TATCTTCCTT AGCTCCTGAA AATCTCGACG GATCCTAACT   121 CAAAATCCAC ACATTATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGC   181 GGCCGCCATA GTGACTGGAT ATGTTGTGTT TTACAGTATT ATGTAGTCTG TTTTTTATGC   241 AAAATCTAAT TTAATATATT GATATTTATA TCATTTTACG TTTCTCGTTC AGCTTTTTTG   301 TACAAACTTG TTGATATCCG GATCCAGGCC TGTCGACGCT AGCATCAGTA ACTCTAAAGT   361 CCTTCTATTC CTTTTTTTAA CCTTTATTTT TGGCGGTTAG TGTTTTGCTC TAGTTACGTT   421 AAAGTGCCTT GGAGAAATGA GGACTACCGT TGTCGCTTCC TAATGTTGCA AGTCCTACCA   481 TCAACAGTGA TGTCAACAAA CAATGAGAGT TTATTACATT ATCGTATATG CTCTAACAAA   541 CCAGTAACGT ACTTGCTATA AACCGCACTA AAACCACTAA ACGGCGGATG AAGGAACTTT   601 TTCAATGTCA GCTACGCATT TATTGGTATC TATTTAACGA CACCTAGAAC TGCCGTGTGG   661 TTTACTTGTG ACCTATTGTA TTATGGTACA CAGGTAAATA CATTTAGTAA CTTAGAATGA   721 TATTCTGATA TAGAGGTTAT ATTGTTACCT ATCACCGAAT TAGCATAAAA GTGAATAGAG   781 AAACGAGGTT TTGTTTATTT CAATTCATAA AATTTAAGCA TTCTATTATT TTTTTTGTTA   841 TTCATTTTAT AACTCTTAGT AATCTACCTA AGTAACTTAT CATGTACAGC TTGACAGAGC   901 AAATCAAACA TGTGATAATT AGTGATACAC AACGTAGTAT GCTATAGCGC ATGCAAGCTT   961 GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA  1021 CAACATACGA GCCGGAAGCA TAAAGTTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC  1081 TCACATTTAT TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CCGGGGAAAC CTGTCGTGCC  1141 AGCTGCATTA ATGAATTCGG CCACGCGCGG GGAGAAGCCG TTGCGTATTG GGCGCTCTCG 1201 GCTCTCGCCT ACATGAACTG CAGTCGCCTT CGGATG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.18

GNP_filter3_RDBDEP_html_220516.pl