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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pHFF1-ccdB2

S. pombe 5 ter end His6-FLAG2-tagged vector with nmt1 promoter

Catalog number RDB06522
Resource name pHFF1-ccdB2
Clone info. S. pombe 5 ter end His6-FLAG2-tagged vector with nmt1 promoter.
Comment Gateway compatible.
Vector backbone pREP1 (Plasmid)
Selectable markers E. coli: Amp^r, Cm^r, ccdB.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
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External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature(s). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Remarks Use Survival2 (Invitrogen) or equivalent to amplify this clone.
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献の引用と、寄託者への謝辞の表明を必要とする。 利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。 寄託者から要請がある場合、理研BRCは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            備考 このクローンの増殖にはSurvival2 (Invitrogen) あるいは同等の宿主を使用してください。

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            RDB06522 pHFF1-ccdB2 DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB06522z.seq

            Original reference

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Expression Vector Backbone of Schizosaccharomyces pombe (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB12286_A3Jm.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: nmt1Fw
            Sequence file: RDB12286_A3Jma.seq check
            >06522_12286_A3Jm_nmt1Fw_C05_08.ab1
                1 TAAATTTAAT TTAATCCAGG AAAAACGTAA CTCTCGGCTA CTGGATGGTT CAGTCACCCA
               61 ACGATTACTG GGGAGAGAAA ACAGGGCAAA AGCAAAGCTT AAAGGAATCC GATTGTCATT
              121 CGGCAATGTG CAGCGAAACT AAAAACCGGA TAATGGACCT GTTAATCGAA ACATTGAAGA
              181 TATATAAAGG AAGAGGAATC CTGGCATATC ATCAATTGAA TAAGTTGAAT TAATTATTTC
              241 AATCTCATTC TCACTTTCTG ACTTATAGTC GCTTTGTTAA AGCTAGCGTC GACAGGCCTG
              301 GATCCTATGC ATCACCACCA TCATCATATG GATTATAAGG ATGATGACGA TAAACCCATG
              361 GACTACAAGG ACGACGATGA CAAAGATATC ACAAGTTTGT ACAAAAAAGC TGAACGAGAA
              421 ACGTAAAATG ATATAAATAT CAATATATTA AATTAGATTT TGCATAAAAA ACAGACTACA
              481 TAATACTGTA AAACACAACA TATCCAGTCA CTATGGCGGC CGCATTAGGC ACCCCAGGCT
              541 TTACACTTTA TGCTTCCGGC TCGTATAATG TGTGGATTTT GAGTTAGGAT CCGTCGAGAT
              601 TTTCAGGAGC TAAGGAAGCT AAAATGGAGA AAAAAATCAC TGGATATACC ACCGTTGATA
              661 TATCCCAATG GCATCGTAAA GAACATTTTG AGGCATTTCA GTCAGTTGCT CAATGTACCT
              721 ATAACCAGAC CGTTCAGCTG GATATTACGG CCTTTTTAAA GACCGTAAAG AAAAATAAGC
              781 ACAAGTTTTA TCCGGCCTTT ATTCACATTC TTGCCCGCCT GATGAATGCT CATCCGGAAT
              841 TCCGTATGGC AATGAAAGAC GGTGAGCTGG TGATATGGGA TAGTGTTCAC CCTTGTTACA
              901 CCGTTTTCCA TGAGCAAACT GAAACGTTTT CATCGCTCTG GAGTGAATAC CACGACGATT
              961 TCCGGCAGTT TCTACACATA TATTCGCAAG ATGTGGCGTG TTACGGTGAA AACCCTGGCC
             1021 TATTTCCCTA AAAGGGTTTA TTTGAGAATA TGTTTTTTCG TCTCAGCCCA AATCCCTGGG
             1081 TGAGTTTCAC CAAGTTTTTG GAATTAACCG TGACAATATG GACACTTTCT TCGTCCCCCG
            1141 AATTTTTCAA
            //
            Primer: nmt1Rv
            Sequence file: RDB12286_A3Jmb.seq check
            >06522_12286_A3Jm_nmt1Rv_D05_11.ab1
                1 AGCCGCCCGG GGCCTCGAGA TCTCATGATA TCACCACTTT GTACAAGAAA GCTGAACGAG
               61 AAACGTAAAA TGATATAAAT ATCAATATAT TAAATTAGAT TTTGCATAAA AAACAGACTA
              121 CATAATACTG TAAAACACAA CATATCCAGT CACTATGGTC GACCTGCAGA CTGGCTGTGT
              181 ATAAGGGAGC CTGACATTTA TATTCCCCAG AACATCAGGT TAATGGCGTT TTTGATGTCA
              241 TTTTCGCGGT GGCTGAGATC AGCCACTTCT TCCCCGATAA CGGAGACCGG CACACTGGCC
              301 ATATCGGTGG TCATCATGCG CCAGCTTTCA TCCCCGATAT GCACCACCGG GTAAAGTTCA
              361 CGGGAGACTT TATCTGACAG CAGACGTGCA CTGGCCAGGG GGATCACCAT CCGTCGCCCG
              421 GGCGTGTCAA TAATATCACT CTGTACATCC ACAAACAGAC GATAACGGCT CTCTCTTTTA
              481 TAGGTGTAAA CCTTAAACTG CATTTCACCA GCCCCTGTTC TCGTCAGCAA AAGAGCCGTT
              541 CATTTCAATA AACCGGGCGA CCTCAGCCAT CCCTTCCTGA TTTTCCGCTT TCCAGCGTTC
              601 GGCACGCAGA CGACGGGCTT CATTCTGCAT GGTTGTGCTT ACCAGACCGG AGATATTGAC
              661 ATCATATATG CCTTGAGCAA CTGATAGCTG TCGCTGTCAA CTGTCACTGT AATACGCTGC
              721 TTCATAGCAT ACCTCTTTTT GACATACTTC GGGTATACAT ATCAGTATAT ATTCTTATAC
              781 CGCAAAAATC AGCGCGCAAA TACGCATACT GTTATCTGGC TTTTAGTAAG CCGGATCCAC
              841 GCGTTTACGC CCCGCCCTGC CACTCATCGC AGTACTGTTG TAATTCATTA AGCATTCTGC
              901 CGACATGGAA AGCCATCACA AACGGCATGA TGAACCTGAA TCGCCAGCGG CATCAGCACC
              961 TTGTCGCCTT GCGTATAATA TTTGCCCATG GGTGAAAACG GGGGCGAAGA AGTTGTCCAT
             1021 ATTGGTCCAC GTTTAAATCA AAACTGGTGA ACTCACCCAG GGATTGGCTG AGACGAAAAA
            1081 CATATTCTCA TAATCCTTCG GAATAGGCCA GGTTTTCACC CGTAAACCAC GGCCCCAAC
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles


            2022.05.18

            GNP_filter3_RDBDEP_html_220516.pl