Please review the QC test results indicated by icon below as well as clone information before placing your order.

pDUAL-HFF31

S. pombe 5 ter end His6-FLAG2-tagged vector with cam1 promoter

Sequence (full)	RDB06532zzk02.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., A., Yeast, 25 (5): 371-376 (2008)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.

Ordering

Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., A., Yeast, 25 (5): 371-376 (2008)) の引用と、寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

References and tips

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB19712_B2J7p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: ADHrev (Pr0048) Region: ADH1 ter,FLAGx2,6xHis,cam1 pro Sequence file: RDB19712_B2J7a.seq
>06532_19712_B2J7_3_ADHrev_A10_01_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNAANTT TCGTTTTAAA ACCTAAGAGT CACTTTAAAA    61 TTTGTATACA CTTATTTTTT TTATAACTTA TTTAATAATA AAAATCATAA ATCATAAGAA   121 ATTCGCTTAT TTAGAAGTGG CGCGCCGGAT CCTCTAGAGT CGAGATCTCA TGATATCTTT   181 GTCATCGTCG TCCTTGTAGT CCATGGGTTT ATCGTCATCA TCCTTATAAT CCATATGATG   241 ATGGTGGTGA TGCATAGGAT CCAGGCCTGT CGACGCTAGC ATCAGTAACT CTAAAGTCCT   301 TCTATTCCTT TTTTTAACCT TTATTTTTGG CGGTTAGTGT TTTGCTCTAG TTACGTTAAA   361 GTGCCTTGGA GAAATGAGGA CTACCGTTGT CGCTTCCTAA TGTTGCAAGT CCTACCATCA   421 ACAGTGATGT CAACAAACAA TGAGAGTTTA TTACATTATC GTATATGCTC TAACAAACCA   481 GTAACGTACT TGCTATAAAC CGCACTAAAA CCACTAAACG GCGGATGAAG GAACTTTTTC   541 AATGTCAGCT ACGCATTTAT TGGTATCTAT TTAACGACAC CTAGAACTGC CGTGTGGTTT   601 ACTTGTGACC TATTGTATTA TGGTACACAG GTAAATACAT TTAGTAACTT AGAATGATAT   661 TCTGATATAG AGGTTATATT GTTACCTATC ACCGAATTAG CATAAAAGTG AATAGAGAAA   721 CGAGGTTTTG TTTATTTCAA TTCATAAAAT TTAAGCNTTC TATTATTTTT TTTGTTATTC   781 ATTTTATAAC TCTTAGTAAT CTACCTAAGT AACTTATCAT GTACAGCTTG ACAGAGCAAA   841 TCAAACATGT GATAATTAGT GATACACNAC GTAGTATGCT ATAGCGCANG CNAANCTTGG   901 CGTAANNCAT GGNCATAGCT GNTTTNCTGT GTGAANNTGN TATNCGCTNA CAATTCCNCA   961 CAACNNACNA NCCNGAANNN NNAAGNGNAA ANNNNNGGGT GNCCTAANNN ANTGANCNNN 1021 NNNNNNN //
Primer: LEU1pro_F (Pr0370) Region: leu pro,Leu1-5'ORF,ura4 ter Sequence file: RDB19712_B2J7b.seq
>06532_19712_B2J7_3_LEU1pro_F_B10_02_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NTTGAGTTTC CCGAANCCNG GCATTTTTGC GGTAGGGTCT    61 GAAACTTTCA CGAAACCATT TCACAACAAA ACTTCTTCAA CAACATGTGT GCAAAGAAAA   121 TCGTCGTCTT ACCAGGAGAC CATATTGGCC CTGAAATTGT TGCTTCTGCC TTGGAGGTTT   181 TGAAAGTCGT TGAGAAGAAG CGACCTGAGT TAAAACTCGA GTTTGAAGAA CACAAGATTG   241 GAGGTGCCTC TATTGATGCC TATGGAACCC CTTTGACTGA CGAGACTGTG AAGGCTTGTT   301 TGGAAGCTGA CGGTGTTCTT TTGGGTGCCG TTGGTGGTCC TGAATGGACC AACCCCAATT   361 GTCGTCCTGA GCAAGGTTTA TTGAAGCTTC GTAAGAGTAT GGGTGTTTGG GCCAACCTTC   421 GACCTTGCAA CTTTGCCAGC AAGTCTTTAG TCAAGTACAG CCCTTTGAAG CCTGAAATCG   481 TTGAAGGTGT CGATTTTTGT GTTGTACGAG AACTTACTGG AGGTTGTTAC TTTGGTGAGC   541 GCACTGAGGA CAACGGATCG GGTTATGCTA TGGACACTTG GCCTTACAGT TTGGAAGAAG   601 TTTCTCGTAT TGCTCGTTTG GCGGCCGCGG GCCCAACACC AATGTTTATA ACCAAGTTTT   661 ATCTTGTTTG TCTACATGGT ATTTTACATT CATCTACATA CATCTTTCAT TGGCTTTGTA   721 CATAGTTATC ATTACAAGTC TAAAAAAATT CACTCTTTTC TTATTCAATG TCNATCCAAG   781 AGAAAAGANT GTGGTAATGN TGTAGGANCA TGTTNNATAA ATTACTATAG CAAATTACTT   841 TTTATTCNNA ANGTGTTTAT CTATAATAGT TAATANTTNA NNNGCTNCAT AAAATTTTAN   901 NNAANAGTAC TTGTATACTA NTTCNNAATG NCNTCTGANN TAAAACGCNT AGGAAAACNN   961 NNNNANCNNG GCATCNACTT TTTTCNANNA NCNNNNNNNA AANANTTTNN ATTANNCNTT 1021 TTTTTNNNTG NTNNANANNN NTTNNCNNNN NNNNNN //
Primer: URA4pro_F (Pr0371) Region: ura4 pro,ura4 ORF Sequence file: RDB19712_B2J7c.seq
>06532_19712_B2J7_3_URA4pro_F_C10_03_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNG ANNCTCTTTT TTGNTTGGAT CGAAATTAAA    61 GGTTTAAAAG CAAAGTTATG GATGCTAGAG TATTTCAAAG CTATTCAGCT AGAGCTGAGG   121 GGATGAAAAA TCCCATTGCC AAGGAATTGT TGGCTTTGAT GGAAGAAAAG CAAAGCAACT   181 TGTCAGTCGC GGTCGATTTG ACGAAGAAAT CCGAAATCTT AGAATTGGTA GATAAAATTG   241 GACCCTATGT CTGTGTTATC AAGACACATA TTGACGTTGT CGAGGATTTC GACCAGGATA   301 TGGTAGAAAA ACTGGTGGCC TTAGGTAAAA AGCATCGTTT TCTTATCTTT GAGGATCGCA   361 AATTCGCAGA CATTGGAAAT ACCGTCAAGC TACAATATGC ATCTGGTGTG TACAAAATTG   421 CTTCTTGGGC TCATATCACA AATTGCCATA CAGTGCCAGG CGAGGGTATT ATACAAGGCC   481 TCAAAGAAGT TGGTTTACCT TTGGGACGTG GTCTCTTGCT TTTGGCTGAA ATGTCTTCCA   541 AAGGCTCTTT GGCTACTGGT TCCTACACAG AGAAAACCTT AGAATGGTTT GAGAAGCATA   601 CCGATTTTTG CTTTGGCTTT ATAGCTGGTC GTCGATTTCC TAACCTTCAA AGCGACTACA   661 TAACTATGTC CCCTGGTATC GGCTTGGATG TTAAAGGAGA CGGGCTGGGA CAGCAATATC   721 GTACTCCTGA AGAAGTGATT GTAAACTGCG GTAGCGACAT CATCATTGTT GGTCGTGGAG   781 TCTATGGAGC TGGTCGTAAT CCTGTTGTCG AANCCNAGAG ATATAGAGAA NCTGGTTGGA   841 NNGCATATCA NNNAAGACTT TCTCANCATN NAAAAAAGAC NNNNGTAAAA TTTTTTNNGG   901 TNNGNTANTG NAAAAGTCGA TGCCTTGTTT GCNNTNGTTT TNNNANGCGT TTTATGTCAN   961 AANGGNNNTT NNAANNNNGT ANNCANGTNN TCNTTTNGNN AAAATTTTAT GTANCGNCNN 1021 AANNNNNTAN CNNNTTANNN NANNAACNNC NTNNGGNANN NAAAAANNNN NNNNNN //
Primer: pBAC_lac_F (Pr0544) Region: leu1-3',ars1 Sequence file: RDB19712_B2J7d.seq
>06532_19712_B2J7_3_pBAC_lac_F_D10_04_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNTNNANNN NNGGNNCGCT ACCGTGAATG GGCTCNACCA    61 AACAATGAAC CTTTTCTTCT GATTTTCCTA CCACACCGGA AAGGGAGGCA GAAGGCAAAA   121 GGCCCAAGCT ACCAGGAATG ACAGAAGCCT CATCTGAAAT AATGTCACCA AACAAGTTGT   181 CAGTCAAAAC AACACCGTTA AGTGTACGAG GGCTCTTGAC CAAAAGCATG GCTGCGGAGT   241 CAATGAGCTG GTTTTTTAAG GTAAGGTGAG GATATTCCTC CTTAAAAATC TTAGCTACAG   301 TCTTGCGCCA AAGACGAGAA GTTGCCAAAA CATTAGCTTT GTCGAGTAAT GTGACGGGAG   361 CAGGAGGGTT GGAAGTTTCA GCTAAGCGGC CGCGGGCCCA ACCTTCCAAT TCATTAAATC   421 GAGTCCTCCA TCAGATGATT TACAAGGTTT TGCATAGAAT CCCTAAGTAA GAGAAGTTGT   481 TAGTGATTTC TTCAAGTTTA CTAAAATTTA CATACAAATG GATGATCTCT TCTCCATTGT   541 TTCCTTTCTT CTTGCAAACG CGTTTTACAA AGAGATGACA TGCTATTTTC TGATTATTTT   601 TTTCTATAGT TTTCTATTTT GTACGTCCTT GGGAGTCCGA AATGTAAAAT CGGGGTATGG   661 TTAGTAGCGG CAGCGTTCAT TATGGTATAT AAAAGATAAA AAGTAGGGGC AAGCGAGACA   721 GAATGGGATA CAAGGGCATC GTCTATAATT ATAGCTAAAA ATTGTATTTT AATTTGTATT   781 TTTTGTAATT TTATTTCTCA TTGTTTTACT TAAAATGAAT GCGAATTAGA GAAAACTTCA   841 ACGAAATGTC AAAATAAGCT CAGCAAAATA TACNATTTTA NGGAAAGCGA TCAGCAACTT   901 ATTCTCCGTN NTANANTCNT TTNCNTCNTA AATTCCNACN NTGTAACTGN NNATTGNNTT   961 TTCTCTANAT GCTAATAAAT TGANNNAANG NNNNNNATAN NNNNNNTTTT NANAANNNGN  1021 AAACTNNNNN NNNAANNNNN NNNGNNNANA NNNNNTNNNN NNTTNNNNNA NNNNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl