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pDUAL-HFF31 (#RDB06532)

S. pombe 5 ter end His6-FLAG2-tagged vector with cam1 promoter

Clone info. S. pombe 5 ter end His6-FLAG2-tagged vector with cam1 promoter.
Comment Multicopy marker, ura4+; Integration marker, leu1+. Gateway incompatible.
Vector backbone pDUAL (RDB06178) (Plasmid)
Selectable markers Amp^r
Reference of insert sequence AB364120 (DDBJ accession number)
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
 
Sequence (full) RDB06532zzk02.seq provided by the depositor

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., A., Yeast, 25 (5): 371-376 (2008)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., A., Yeast, 25 (5): 371-376 (2008)) の引用と、寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06532 pDUAL-HFF31 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pDUAL-HFF31 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06532).

Reference section:

Matsuyama, A., Yoshida, M., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913. [link to RRC of NBRP]
Matsuyama, A., Shirai, A., Yoshida, M., A series of promoters for constitutive expression of heterologous genes in fission yeast. Yeast, 25 (5):371-376 (2008). PMID 18437702. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB19712_B2J7p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: ADHrev (Pr0048)
Region: ADH1 ter,FLAGx2,6xHis,cam1 pro
Sequence file: RDB19712_B2J7a.seq check
>06532_19712_B2J7_3_ADHrev_A10_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNAANTT TCGTTTTAAA ACCTAAGAGT CACTTTAAAA
   61 TTTGTATACA CTTATTTTTT TTATAACTTA TTTAATAATA AAAATCATAA ATCATAAGAA
  121 ATTCGCTTAT TTAGAAGTGG CGCGCCGGAT CCTCTAGAGT CGAGATCTCA TGATATCTTT
  181 GTCATCGTCG TCCTTGTAGT CCATGGGTTT ATCGTCATCA TCCTTATAAT CCATATGATG
  241 ATGGTGGTGA TGCATAGGAT CCAGGCCTGT CGACGCTAGC ATCAGTAACT CTAAAGTCCT
  301 TCTATTCCTT TTTTTAACCT TTATTTTTGG CGGTTAGTGT TTTGCTCTAG TTACGTTAAA
  361 GTGCCTTGGA GAAATGAGGA CTACCGTTGT CGCTTCCTAA TGTTGCAAGT CCTACCATCA
  421 ACAGTGATGT CAACAAACAA TGAGAGTTTA TTACATTATC GTATATGCTC TAACAAACCA
  481 GTAACGTACT TGCTATAAAC CGCACTAAAA CCACTAAACG GCGGATGAAG GAACTTTTTC
  541 AATGTCAGCT ACGCATTTAT TGGTATCTAT TTAACGACAC CTAGAACTGC CGTGTGGTTT
  601 ACTTGTGACC TATTGTATTA TGGTACACAG GTAAATACAT TTAGTAACTT AGAATGATAT
  661 TCTGATATAG AGGTTATATT GTTACCTATC ACCGAATTAG CATAAAAGTG AATAGAGAAA
  721 CGAGGTTTTG TTTATTTCAA TTCATAAAAT TTAAGCNTTC TATTATTTTT TTTGTTATTC
  781 ATTTTATAAC TCTTAGTAAT CTACCTAAGT AACTTATCAT GTACAGCTTG ACAGAGCAAA
  841 TCAAACATGT GATAATTAGT GATACACNAC GTAGTATGCT ATAGCGCANG CNAANCTTGG
  901 CGTAANNCAT GGNCATAGCT GNTTTNCTGT GTGAANNTGN TATNCGCTNA CAATTCCNCA
  961 CAACNNACNA NCCNGAANNN NNAAGNGNAA ANNNNNGGGT GNCCTAANNN ANTGANCNNN
1021 NNNNNNN
//
Primer: LEU1pro_F (Pr0370)
Region: leu pro,Leu1-5'ORF,ura4 ter
Sequence file: RDB19712_B2J7b.seq check
>06532_19712_B2J7_3_LEU1pro_F_B10_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NTTGAGTTTC CCGAANCCNG GCATTTTTGC GGTAGGGTCT
   61 GAAACTTTCA CGAAACCATT TCACAACAAA ACTTCTTCAA CAACATGTGT GCAAAGAAAA
  121 TCGTCGTCTT ACCAGGAGAC CATATTGGCC CTGAAATTGT TGCTTCTGCC TTGGAGGTTT
  181 TGAAAGTCGT TGAGAAGAAG CGACCTGAGT TAAAACTCGA GTTTGAAGAA CACAAGATTG
  241 GAGGTGCCTC TATTGATGCC TATGGAACCC CTTTGACTGA CGAGACTGTG AAGGCTTGTT
  301 TGGAAGCTGA CGGTGTTCTT TTGGGTGCCG TTGGTGGTCC TGAATGGACC AACCCCAATT
  361 GTCGTCCTGA GCAAGGTTTA TTGAAGCTTC GTAAGAGTAT GGGTGTTTGG GCCAACCTTC
  421 GACCTTGCAA CTTTGCCAGC AAGTCTTTAG TCAAGTACAG CCCTTTGAAG CCTGAAATCG
  481 TTGAAGGTGT CGATTTTTGT GTTGTACGAG AACTTACTGG AGGTTGTTAC TTTGGTGAGC
  541 GCACTGAGGA CAACGGATCG GGTTATGCTA TGGACACTTG GCCTTACAGT TTGGAAGAAG
  601 TTTCTCGTAT TGCTCGTTTG GCGGCCGCGG GCCCAACACC AATGTTTATA ACCAAGTTTT
  661 ATCTTGTTTG TCTACATGGT ATTTTACATT CATCTACATA CATCTTTCAT TGGCTTTGTA
  721 CATAGTTATC ATTACAAGTC TAAAAAAATT CACTCTTTTC TTATTCAATG TCNATCCAAG
  781 AGAAAAGANT GTGGTAATGN TGTAGGANCA TGTTNNATAA ATTACTATAG CAAATTACTT
  841 TTTATTCNNA ANGTGTTTAT CTATAATAGT TAATANTTNA NNNGCTNCAT AAAATTTTAN
  901 NNAANAGTAC TTGTATACTA NTTCNNAATG NCNTCTGANN TAAAACGCNT AGGAAAACNN
  961 NNNNANCNNG GCATCNACTT TTTTCNANNA NCNNNNNNNA AANANTTTNN ATTANNCNTT
1021 TTTTTNNNTG NTNNANANNN NTTNNCNNNN NNNNNN
//
Primer: URA4pro_F (Pr0371)
Region: ura4 pro,ura4 ORF
Sequence file: RDB19712_B2J7c.seq check
>06532_19712_B2J7_3_URA4pro_F_C10_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNG ANNCTCTTTT TTGNTTGGAT CGAAATTAAA
   61 GGTTTAAAAG CAAAGTTATG GATGCTAGAG TATTTCAAAG CTATTCAGCT AGAGCTGAGG
  121 GGATGAAAAA TCCCATTGCC AAGGAATTGT TGGCTTTGAT GGAAGAAAAG CAAAGCAACT
  181 TGTCAGTCGC GGTCGATTTG ACGAAGAAAT CCGAAATCTT AGAATTGGTA GATAAAATTG
  241 GACCCTATGT CTGTGTTATC AAGACACATA TTGACGTTGT CGAGGATTTC GACCAGGATA
  301 TGGTAGAAAA ACTGGTGGCC TTAGGTAAAA AGCATCGTTT TCTTATCTTT GAGGATCGCA
  361 AATTCGCAGA CATTGGAAAT ACCGTCAAGC TACAATATGC ATCTGGTGTG TACAAAATTG
  421 CTTCTTGGGC TCATATCACA AATTGCCATA CAGTGCCAGG CGAGGGTATT ATACAAGGCC
  481 TCAAAGAAGT TGGTTTACCT TTGGGACGTG GTCTCTTGCT TTTGGCTGAA ATGTCTTCCA
  541 AAGGCTCTTT GGCTACTGGT TCCTACACAG AGAAAACCTT AGAATGGTTT GAGAAGCATA
  601 CCGATTTTTG CTTTGGCTTT ATAGCTGGTC GTCGATTTCC TAACCTTCAA AGCGACTACA
  661 TAACTATGTC CCCTGGTATC GGCTTGGATG TTAAAGGAGA CGGGCTGGGA CAGCAATATC
  721 GTACTCCTGA AGAAGTGATT GTAAACTGCG GTAGCGACAT CATCATTGTT GGTCGTGGAG
  781 TCTATGGAGC TGGTCGTAAT CCTGTTGTCG AANCCNAGAG ATATAGAGAA NCTGGTTGGA
  841 NNGCATATCA NNNAAGACTT TCTCANCATN NAAAAAAGAC NNNNGTAAAA TTTTTTNNGG
  901 TNNGNTANTG NAAAAGTCGA TGCCTTGTTT GCNNTNGTTT TNNNANGCGT TTTATGTCAN
  961 AANGGNNNTT NNAANNNNGT ANNCANGTNN TCNTTTNGNN AAAATTTTAT GTANCGNCNN
1021 AANNNNNTAN CNNNTTANNN NANNAACNNC NTNNGGNANN NAAAAANNNN NNNNNN
//
Primer: pBAC_lac_F (Pr0544)
Region: leu1-3',ars1
Sequence file: RDB19712_B2J7d.seq check
>06532_19712_B2J7_3_pBAC_lac_F_D10_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNTNNANNN NNGGNNCGCT ACCGTGAATG GGCTCNACCA
   61 AACAATGAAC CTTTTCTTCT GATTTTCCTA CCACACCGGA AAGGGAGGCA GAAGGCAAAA
  121 GGCCCAAGCT ACCAGGAATG ACAGAAGCCT CATCTGAAAT AATGTCACCA AACAAGTTGT
  181 CAGTCAAAAC AACACCGTTA AGTGTACGAG GGCTCTTGAC CAAAAGCATG GCTGCGGAGT
  241 CAATGAGCTG GTTTTTTAAG GTAAGGTGAG GATATTCCTC CTTAAAAATC TTAGCTACAG
  301 TCTTGCGCCA AAGACGAGAA GTTGCCAAAA CATTAGCTTT GTCGAGTAAT GTGACGGGAG
  361 CAGGAGGGTT GGAAGTTTCA GCTAAGCGGC CGCGGGCCCA ACCTTCCAAT TCATTAAATC
  421 GAGTCCTCCA TCAGATGATT TACAAGGTTT TGCATAGAAT CCCTAAGTAA GAGAAGTTGT
  481 TAGTGATTTC TTCAAGTTTA CTAAAATTTA CATACAAATG GATGATCTCT TCTCCATTGT
  541 TTCCTTTCTT CTTGCAAACG CGTTTTACAA AGAGATGACA TGCTATTTTC TGATTATTTT
  601 TTTCTATAGT TTTCTATTTT GTACGTCCTT GGGAGTCCGA AATGTAAAAT CGGGGTATGG
  661 TTAGTAGCGG CAGCGTTCAT TATGGTATAT AAAAGATAAA AAGTAGGGGC AAGCGAGACA
  721 GAATGGGATA CAAGGGCATC GTCTATAATT ATAGCTAAAA ATTGTATTTT AATTTGTATT
  781 TTTTGTAATT TTATTTCTCA TTGTTTTACT TAAAATGAAT GCGAATTAGA GAAAACTTCA
  841 ACGAAATGTC AAAATAAGCT CAGCAAAATA TACNATTTTA NGGAAAGCGA TCAGCAACTT
  901 ATTCTCCGTN NTANANTCNT TTNCNTCNTA AATTCCNACN NTGTAACTGN NNATTGNNTT
  961 TTCTCTANAT GCTAATAAAT TGANNNAANG NNNNNNATAN NNNNNNTTTT NANAANNNGN
 1021 AAACTNNNNN NNNAANNNNN NNNGNNNANA NNNNNTNNNN NNTTNNNNNA NNNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913. [link to RRC of NBRP]
original Matsuyama, A., A series of promoters for constitutive expression of heterologous genes in fission yeast. Yeast, 25 (5):371-376 (2008). PMID 18437702. [link to RRC of NBRP]