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pDUAL-HFF61 (#RDB06534)

S. pombe 5 ter end His6-FLAG2-tagged vector with ef1a-c promoter

Clone info. S. pombe 5 ter end His6-FLAG2-tagged vector with ef1a-c promoter.
Comment Multicopy marker, ura4+; Integration marker, leu1+. Gateway incompatible.
Vector backbone pDUAL (RDB06178) (Plasmid)
Selectable markers Amp^r
Reference of insert sequence AB364122 (DDBJ accession number)
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
 
Sequence RDB06534z.seq

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., A., Yeast, 25 (5): 371-376 (2008)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., A., Yeast, 25 (5): 371-376 (2008)) の引用と、寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06534 pDUAL-HFF61 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pDUAL-HFF61 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06534).

Reference section:

Matsuyama, A., Yoshida, M., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913. [link to RRC of NBRP]
Matsuyama, A., Shirai, A., Yoshida, M., A series of promoters for constitutive expression of heterologous genes in fission yeast. Yeast, 25 (5):371-376 (2008). PMID 18437702. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12779_A4Dp.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: ADHrev
Sequence file: RDB12779_A4Dpa.seq check
>06534_12779_A4Dp_ADHrev_H08_23.ab1
    1 TGAAGGGGTT CCTTCAAAGA ATAAGAATTT TCGTTTTAAA ACCTAAGAGT CACTTTAAAA
   61 TTTGTATACA CTTATTTTTT TTATAACTTA TTTAATAATA AAAATCATAA ATCATAAGAA
  121 ATTCGCTTAT TTAGAAGTGG CGCGCCGGAT CCTCTAGAGT CGAGATCTCA TGATATCTTT
  181 GTCATCGTCG TCCTTGTAGT CCATGGGTTT ATCGTCATCA TCCTTATAAT CCATATGATG
  241 ATGGTGGTGA TGCATAGGAT CCAGGCCTGT CGACGCTAGC GTGAGTTCGA TGAAACAGAC
  301 TCTAGGTAGA AGCGAAGGGC GCCAGCGCCT ACATATATAC TTGCGCAAAA AAAGCAGTCA
  361 CATAACGTTC GTTACGTCTC GAAAGTTTTT TTCTTTTTTT CCGGCTTAAA ATATTGTCGT
  421 GAGCCTATCA GCGAACGAAT TTTGTTTGCG CAGGAAAGTC ATGATGTCAT TTAAAATTTT
  481 TCTTTGCTTC TTAACCTTTT GTCTTTAGAG TACACTTAGC CGAATTTGCC ACCGCAATAA
  541 ACATTAGCTT AGCGAACGTC GTTCTTGGCG ATTTTGATTT ATTGGGCGGA AACGATCAAC
  601 GCTGTATATA TGAAATGGAT CGTTTTTCGT TAATTTAAAA ATATATATCT GGTTTATTAA
  661 ATGATGAAAA ATATTTAGTT GTATCATTGA ACCATTTTTG ATGGCCGTTA TCAGATCGGA
  721 TTGAGATTGT GGACTGAGAT TTTCGCTGAT ATACCCACCT TAGCTGCTTT TGTTTGTTCT
  781 TTGTCCGTCA CCACAACGGT AATAGTTTTG ATGGCTTTGC TTTTGGAGGG TACTTCTTTG
  841 GCCCGGTATG TCTTTTTTAT CGTGTATATA TAGTTTTGTC TAACAGTTGA ATTAGCAAAG
  901 TTCGAGAAGA ACTTCGGGAG CAGATCTCCT CGATTAAATC TGTTGACCCT TATTTCAACG
  961 TTTCTTTAAA AATCATTCAA GTCGGAGGTA GAGAGGACTC AAATGTATAT GTGCGCATGA
 1021 AAACTCGTGC AGCTAATGAG GTATGAGTGA AGGCGAGAAG GTCTCCTAAA TGATTGAAAT
 1081 GCATGCAAGC TTGGCGTAAT CATGTTCATA GCTGTTTCCT GTGTTGAATT GTTATCCGCT
 1141 CCACATCCAC ACAACAATAC GAGTCGGAGC CATAAAGTGT AAAGGCCTGG GGGGTGCCTA
1201 TTGA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913. [link to RRC of NBRP]
original Matsuyama, A., A series of promoters for constitutive expression of heterologous genes in fission yeast. Yeast, 25 (5):371-376 (2008). PMID 18437702. [link to RRC of NBRP]