Please review the QC test results indicated by icon below as well as clone information before placing your order.

pDUAL-FFH31

S. pombe 3 ter end FLAG2-His6-tagged vector with cam1 promoter

Sequence (full)	RDB06536zzk02.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., A., Yeast, 25 (5): 371-376 (2008)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., A., Yeast, 25 (5): 371-376 (2008)) の引用と、寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB19711_B2J7p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: ADHrev (Pr0048) Region: ADH1 ter,6xHis,FLAGx2,cam1 pro Sequence file: RDB19711_B2J7a.seq
>06536_19711__B2J7_2_ADHrev_A11_02_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NGANNTTTCN NTTTAANACC TAAGAGTCAC TTTAAAATTT    61 GTATACACTT ATTTTTTTTA TAACTTATTT AATAATAAAA ATCATAAATC ATAAGAAATT   121 CGCTTATTTA GAAGTGGCGC GCCGGATCCT CTAGAGTCGA GATCTCAGTG GTGATGGTGG   181 TGATGTTTGT CATCGTCGTC CTTGTAGTCC ATGGGTTTAT CGTCATCATC CTTATAATCC   241 ATGATATCCG GATCCAGGCC TGTCGACGCT AGCATCAGTA ACTCTAAAGT CCTTCTATTC   301 CTTTTTTTAA CCTTTATTTT TGGCGGTTAG TGTTTTGCTC TAGTTACGTT AAAGTGCCTT   361 GGAGAAATGA GGACTACCGT TGTCGCTTCC TAATGTTGCA AGTCCTACCA TCAACAGTGA   421 TGTCAACAAA CAATGAGAGT TTATTACATT ATCGTATATG CTCTAACAAA CCAGTAACGT   481 ACTTGCTATA AACCGCACTA AAACCACTAA ACGGCGGATG AAGGAACTTT TTCAATGTCA   541 GCTACGCATT TATTGGTATC TATTTAACGA CACCTAGAAC TGCCGTGTGG TTTACTTGTG   601 ACCTATTGTA TTATGGTACA CAGGTAAATA CATTTAGTAA CTTAGAATGA TATTCTGATA   661 TAGAGGTTAT ATTGTTACCT ATCACCGAAT TAGCATAAAA GTGAATAGAG AAACGAGGTT   721 TTGTTTATTT CAATTCATAA AATTTAAGCA TTCTATTATT TTTTTTGTTA TTCATTTTAT   781 AACTCTTAGT AATCTACCTA AGTAACTTAT CATGTACAGC TTGACAGANC ANATCANACA   841 TGTGATAATT AGTGATACAC AACGTANTAT GCTATAGCGC ATGCAAGCTT GGCGTAATCA   901 TNGNCATANC NNTTTNCTGT GNGAAATNGT TATNCGCTCN CNNTNNCNCA CAANATACNA 961 GCNNGAAGCN NAAANNGNAA AGCCNNGNGN NNNNNNANNN NNNNNNN //
Primer: LEU1pro_F (Pr0370) Region: leu1 pro,leu1-5'ORF,ura4 ter Sequence file: RDB19711_B2J7b.seq
>06536_19711__B2J7_2_LEU1pro_F_G11_20_ABI24.ab1     1 NNNNNNNNNN NNNNNNNTTG ANTTTCCCGA AACCNGGCAT TTTTGCGGTA GGGTCTGAAA    61 CTTTCACGAA ACCATTTCAC AACAAAACTT CTTCAACAAC ATGTGTGCAA AGAAAATCGT   121 CGTCTTACCA GGAGACCATA TTGGCCCTGA AATTGTTGCT TCTGCCTTGG AGGTTTTGAA   181 AGTCGTTGAG AAGAAGCGAC CTGAGTTAAA ACTCGAGTTT GAAGAACACA AGATTGGAGG   241 TGCCTCTATT GATGCCTATG GAACCCCTTT GACTGACGAG ACTGTGAAGG CTTGTTTGGA   301 AGCTGACGGT GTTCTTTTGG GTGCCGTTGG TGGTCCTGAA TGGACCAACC CCAATTGTCG   361 TCCTGAGCAA GGTTTATTGA AGCTTCGTAA GAGTATGGGT GTTTGGGCCA ACCTTCGACC   421 TTGCAACTTT GCCAGCAAGT CTTTAGTCAA GTACAGCCCT TTGAAGCCTG AAATCGTTGA   481 AGGTGTCGAT TTTTGTGTTG TACGAGAACT TACTGGAGGT TGTTACTTTG GTGAGCGCAC   541 TGAGGACAAC GGATCGGGTT ATGCTATGGA CACTTGGCCT TACAGTTTGG AAGAAGTTTC   601 TCGTATTGCT CGTTTGGCGG CCGCGGGCCC AACACCAATG TTTATAACCA AGTTTTATCT   661 TGTTTGTCTA CATGGTATTT TACATTCATC TACATACATC TTTCATTGGC TTTGTACATA   721 GTTATCATTA CAAGTCTAAA AAAATTCACT CTTTTCTTAT TCAATGTCAA TCCAAGAGAA   781 AAGATTGTGG TAATGTTGTA NGAGCATGTT TAATAAATTA CTATAGCAAA TTACTTTTTA   841 TTNNNNAGGT GTTTATCTAT AATANTTAAT ATTTTAGTCG CTACATAAAA TTTTANNNAA   901 GAGTANTTGT ATACTAATTC TAAANNNCTT CTGANNTAAA ACGCNTANNN AAACAAACGC 961 NAACNNNNNA TCNNCTTTTT TCNNNNANNN NNCNAAAAAA ANNTTNNNNN NNNNN //
Primer: URA4pro_F (Pr0371) Region: ura4 pro,ura4 ORF Sequence file: RDB19711_B2J7c.seq
>06536_19711__B2J7_2_URA4pro_F_H11_23_ABI24.ab1     1 NNNNNNNNNN NNNNNANTGT CCNACNNNGA NCCTCTTTTT TGCTTGGNNC GAAATTAAAG    61 GTTTAAAAGC AAAGTTATGG ATGCTAGAGT ATTTCAAAGC TATTCAGCTA GAGCTGAGGG   121 GATGAAAAAT CCCATTGCCA AGGAATTGTT GGCTTTGATG GAAGAAAAGC AAAGCAACTT   181 GTCAGTCGCG GTCGATTTGA CGAAGAAATC CGAAATCTTA GAATTGGTAG ATAAAATTGG   241 ACCCTATGTC TGTGTTATCA AGACACATAT TGACGTTGTC GAGGATTTCG ACCAGGATAT   301 GGTAGAAAAA CTGGTGGCCT TAGGTAAAAA GCATCGTTTT CTTATCTTTG AGGATCGCAA   361 ATTCGCAGAC ATTGGAAATA CCGTCAAGCT ACAATATGCA TCTGGTGTGT ACAAAATTGC   421 TTCTTGGGCT CATATCACAA ATTGCCATAC AGTGCCAGGC GAGGGTATTA TACAAGGCCT   481 CAAAGAAGTT GGTTTACCTT TGGGACGTGG TCTCTTGCTT TTGGCTGAAA TGTCTTCCAA   541 AGGCTCTTTG GCTACTGGTT CCTACACAGA GAAAACCTTA GAATGGTTTG AGAAGCATAC   601 CGATTTTTGC TTTGGCTTTA TAGCTGGTCG TCGATTTCCT AACCTTCAAA GCGACTACAT   661 AACTATGTCC CCTGGTATCG GCTTGGATGT TAAAGGAGAC GGGCTGGGAC AGCAATATCG   721 TACTCCTGAA GAAGTGATTG TAAACTGCGG TAGCGACATC ATCATTGTTG GTCGTGGAGT   781 CTATGGAGCT GGTCGTAATC CTGTTGTCGA ANCCNAGAGA NATAGAGAAG CTGGTTGGAA   841 NGCATATCAN CAAAGACTTT CTCAGCATTA AAAAAAAGAC TAATGNNAAA TTTTTTTTGG   901 NTGGNTATTG AAAAAGNNNA NGCCNNGNTT GCGTTTGNTT TNNNNNNNNT TTNNTGTCNN   961 ANNNNNNTTA NANNTNNNNN NNNANNNNNN NNTNNNNNAA NNTTNNNGNN NNNNNTNANN 1021 NNNNNNNN //
Primer: pBAC_lac_F (Pr0544) Region: leu-3',ars1 Sequence file: RDB19711_B2J7d.seq
>06536_19711_B2J7_2_pBAC_lac_F_H10_08_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNCTCGG GNNCGCTACC GTGNATGGGC TCAACCAAAC    61 AATGAACCTT TTCTTCTGAT TTTCCTACCA CACCGGAAAG GGAGGCAGAA GGCAAAAGGC   121 CCAAGCTACC AGGAATGACA GAAGCCTCAT CTGAAATAAT GTCACCAAAC AAGTTGTCAG   181 TCAAAACAAC ACCGTTAAGT GTACGAGGGC TCTTGACCAA AAGCATGGCT GCGGAGTCAA   241 TGAGCTGGTT TTTTAAGGTA AGGTGAGGAT ATTCCTCCTT AAAAATCTTA GCTACAGTCT   301 TGCGCCAAAG ACGAGAAGTT GCCAAAACAT TAGCTTTGTC GAGTAATGTG ACGGGAGCAG   361 GAGGGTTGGA AGTTTCAGCT AAGCGGCCGC GGGCCCAACC TTCCAATTCA TTAAATCGAG   421 TCCTCCATCA GATGATTTAC AAGGTTTTGC ATAGAATCCC TAAGTAAGAG AAGTTGTTAG   481 TGATTTCTTC AAGTTTACTA AAATTTACAT ACAAATGGAT GATCTCTTCT CCATTGTTTC   541 CTTTCTTCTT GCAAACGCGT TTTACAAAGA GATGACATGC TATTTTCTGA TTATTTTTTT   601 CTATAGTTTT CTATTTTGTA CGTCCTTGGG AGTCCGAAAT GTAAAATCGG GGTATGGTTA   661 GTAGCGGCAG CGTTCATTAT GGTATATAAA AGATAAAAAG TAGGGGCAAG CGAGACAGAA   721 TGGGATACAA GGGCATCGTC TATAATTATA GCTAAAAATT GTATTTTAAT TTGTATTTTT   781 TGTAATTTTA TTTCTCATTG NTTTACTTAA AATGAATGCG ANTTNNAGAA AACTTCAACG   841 AAATGTCNAA ATAAGCTCAN CAAAATATAC NNTTTTANGG AAAGCGATCA NNNACTTATT   901 CNNCGTTGNA NANTCNTTTT CTTCNTAAAT TCCANCANNG TAACTGNCNN NTNGNNTTTT   961 TCTCTNNNNG CTANTNAAAT TGAAACTNNN GNANNNNNAN TANCACNNGN TTTNNNNNAA  1021 ANTNGNNNNN NNNCNTNNNT NNAAAAAANN NAANGNTNNA NNNNNAANTT NAANNNNTTN 1081 TTNNNNNNAA NNNNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2024.03.26

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