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pDUAL-FFH31 (#RDB06536)

S. pombe 3 ter end FLAG2-His6-tagged vector with cam1 promoter

Clone info. S. pombe 3 ter end FLAG2-His6-tagged vector with cam1 promoter
Comment Multicopy marker, ura4+; Integration marker, leu1+. Gateway incompatible.
Vector backbone pDUAL (RDB06178) (Plasmid)
Selectable markers Amp^r
Reference of insert sequence AB364128 (DDBJ accession number)
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
 
Sequence (full) RDB06536zzk02.seq provided by the depositor

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., A., Yeast, 25 (5): 371-376 (2008)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., A., Yeast, 25 (5): 371-376 (2008)) の引用と、寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06536 pDUAL-FFH31 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pDUAL-FFH31 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06536).

Reference section:

Matsuyama, A., Yoshida, M., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913. [link to RRC of NBRP]
Matsuyama, A., Shirai, A., Yoshida, M., A series of promoters for constitutive expression of heterologous genes in fission yeast. Yeast, 25 (5):371-376 (2008). PMID 18437702. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB19711_B2J7p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: ADHrev (Pr0048)
Region: ADH1 ter,6xHis,FLAGx2,cam1 pro
Sequence file: RDB19711_B2J7a.seq check
>06536_19711__B2J7_2_ADHrev_A11_02_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NGANNTTTCN NTTTAANACC TAAGAGTCAC TTTAAAATTT
   61 GTATACACTT ATTTTTTTTA TAACTTATTT AATAATAAAA ATCATAAATC ATAAGAAATT
  121 CGCTTATTTA GAAGTGGCGC GCCGGATCCT CTAGAGTCGA GATCTCAGTG GTGATGGTGG
  181 TGATGTTTGT CATCGTCGTC CTTGTAGTCC ATGGGTTTAT CGTCATCATC CTTATAATCC
  241 ATGATATCCG GATCCAGGCC TGTCGACGCT AGCATCAGTA ACTCTAAAGT CCTTCTATTC
  301 CTTTTTTTAA CCTTTATTTT TGGCGGTTAG TGTTTTGCTC TAGTTACGTT AAAGTGCCTT
  361 GGAGAAATGA GGACTACCGT TGTCGCTTCC TAATGTTGCA AGTCCTACCA TCAACAGTGA
  421 TGTCAACAAA CAATGAGAGT TTATTACATT ATCGTATATG CTCTAACAAA CCAGTAACGT
  481 ACTTGCTATA AACCGCACTA AAACCACTAA ACGGCGGATG AAGGAACTTT TTCAATGTCA
  541 GCTACGCATT TATTGGTATC TATTTAACGA CACCTAGAAC TGCCGTGTGG TTTACTTGTG
  601 ACCTATTGTA TTATGGTACA CAGGTAAATA CATTTAGTAA CTTAGAATGA TATTCTGATA
  661 TAGAGGTTAT ATTGTTACCT ATCACCGAAT TAGCATAAAA GTGAATAGAG AAACGAGGTT
  721 TTGTTTATTT CAATTCATAA AATTTAAGCA TTCTATTATT TTTTTTGTTA TTCATTTTAT
  781 AACTCTTAGT AATCTACCTA AGTAACTTAT CATGTACAGC TTGACAGANC ANATCANACA
  841 TGTGATAATT AGTGATACAC AACGTANTAT GCTATAGCGC ATGCAAGCTT GGCGTAATCA
  901 TNGNCATANC NNTTTNCTGT GNGAAATNGT TATNCGCTCN CNNTNNCNCA CAANATACNA
961 GCNNGAAGCN NAAANNGNAA AGCCNNGNGN NNNNNNANNN NNNNNNN
//
Primer: LEU1pro_F (Pr0370)
Region: leu1 pro,leu1-5'ORF,ura4 ter
Sequence file: RDB19711_B2J7b.seq check
>06536_19711__B2J7_2_LEU1pro_F_G11_20_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNTTG ANTTTCCCGA AACCNGGCAT TTTTGCGGTA GGGTCTGAAA
   61 CTTTCACGAA ACCATTTCAC AACAAAACTT CTTCAACAAC ATGTGTGCAA AGAAAATCGT
  121 CGTCTTACCA GGAGACCATA TTGGCCCTGA AATTGTTGCT TCTGCCTTGG AGGTTTTGAA
  181 AGTCGTTGAG AAGAAGCGAC CTGAGTTAAA ACTCGAGTTT GAAGAACACA AGATTGGAGG
  241 TGCCTCTATT GATGCCTATG GAACCCCTTT GACTGACGAG ACTGTGAAGG CTTGTTTGGA
  301 AGCTGACGGT GTTCTTTTGG GTGCCGTTGG TGGTCCTGAA TGGACCAACC CCAATTGTCG
  361 TCCTGAGCAA GGTTTATTGA AGCTTCGTAA GAGTATGGGT GTTTGGGCCA ACCTTCGACC
  421 TTGCAACTTT GCCAGCAAGT CTTTAGTCAA GTACAGCCCT TTGAAGCCTG AAATCGTTGA
  481 AGGTGTCGAT TTTTGTGTTG TACGAGAACT TACTGGAGGT TGTTACTTTG GTGAGCGCAC
  541 TGAGGACAAC GGATCGGGTT ATGCTATGGA CACTTGGCCT TACAGTTTGG AAGAAGTTTC
  601 TCGTATTGCT CGTTTGGCGG CCGCGGGCCC AACACCAATG TTTATAACCA AGTTTTATCT
  661 TGTTTGTCTA CATGGTATTT TACATTCATC TACATACATC TTTCATTGGC TTTGTACATA
  721 GTTATCATTA CAAGTCTAAA AAAATTCACT CTTTTCTTAT TCAATGTCAA TCCAAGAGAA
  781 AAGATTGTGG TAATGTTGTA NGAGCATGTT TAATAAATTA CTATAGCAAA TTACTTTTTA
  841 TTNNNNAGGT GTTTATCTAT AATANTTAAT ATTTTAGTCG CTACATAAAA TTTTANNNAA
  901 GAGTANTTGT ATACTAATTC TAAANNNCTT CTGANNTAAA ACGCNTANNN AAACAAACGC
961 NAACNNNNNA TCNNCTTTTT TCNNNNANNN NNCNAAAAAA ANNTTNNNNN NNNNN
//
Primer: URA4pro_F (Pr0371)
Region: ura4 pro,ura4 ORF
Sequence file: RDB19711_B2J7c.seq check
>06536_19711__B2J7_2_URA4pro_F_H11_23_ABI24.ab1
    1 NNNNNNNNNN NNNNNANTGT CCNACNNNGA NCCTCTTTTT TGCTTGGNNC GAAATTAAAG
   61 GTTTAAAAGC AAAGTTATGG ATGCTAGAGT ATTTCAAAGC TATTCAGCTA GAGCTGAGGG
  121 GATGAAAAAT CCCATTGCCA AGGAATTGTT GGCTTTGATG GAAGAAAAGC AAAGCAACTT
  181 GTCAGTCGCG GTCGATTTGA CGAAGAAATC CGAAATCTTA GAATTGGTAG ATAAAATTGG
  241 ACCCTATGTC TGTGTTATCA AGACACATAT TGACGTTGTC GAGGATTTCG ACCAGGATAT
  301 GGTAGAAAAA CTGGTGGCCT TAGGTAAAAA GCATCGTTTT CTTATCTTTG AGGATCGCAA
  361 ATTCGCAGAC ATTGGAAATA CCGTCAAGCT ACAATATGCA TCTGGTGTGT ACAAAATTGC
  421 TTCTTGGGCT CATATCACAA ATTGCCATAC AGTGCCAGGC GAGGGTATTA TACAAGGCCT
  481 CAAAGAAGTT GGTTTACCTT TGGGACGTGG TCTCTTGCTT TTGGCTGAAA TGTCTTCCAA
  541 AGGCTCTTTG GCTACTGGTT CCTACACAGA GAAAACCTTA GAATGGTTTG AGAAGCATAC
  601 CGATTTTTGC TTTGGCTTTA TAGCTGGTCG TCGATTTCCT AACCTTCAAA GCGACTACAT
  661 AACTATGTCC CCTGGTATCG GCTTGGATGT TAAAGGAGAC GGGCTGGGAC AGCAATATCG
  721 TACTCCTGAA GAAGTGATTG TAAACTGCGG TAGCGACATC ATCATTGTTG GTCGTGGAGT
  781 CTATGGAGCT GGTCGTAATC CTGTTGTCGA ANCCNAGAGA NATAGAGAAG CTGGTTGGAA
  841 NGCATATCAN CAAAGACTTT CTCAGCATTA AAAAAAAGAC TAATGNNAAA TTTTTTTTGG
  901 NTGGNTATTG AAAAAGNNNA NGCCNNGNTT GCGTTTGNTT TNNNNNNNNT TTNNTGTCNN
  961 ANNNNNNTTA NANNTNNNNN NNNANNNNNN NNTNNNNNAA NNTTNNNGNN NNNNNTNANN
1021 NNNNNNNN
//
Primer: pBAC_lac_F (Pr0544)
Region: leu-3',ars1
Sequence file: RDB19711_B2J7d.seq check
>06536_19711_B2J7_2_pBAC_lac_F_H10_08_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNCTCGG GNNCGCTACC GTGNATGGGC TCAACCAAAC
   61 AATGAACCTT TTCTTCTGAT TTTCCTACCA CACCGGAAAG GGAGGCAGAA GGCAAAAGGC
  121 CCAAGCTACC AGGAATGACA GAAGCCTCAT CTGAAATAAT GTCACCAAAC AAGTTGTCAG
  181 TCAAAACAAC ACCGTTAAGT GTACGAGGGC TCTTGACCAA AAGCATGGCT GCGGAGTCAA
  241 TGAGCTGGTT TTTTAAGGTA AGGTGAGGAT ATTCCTCCTT AAAAATCTTA GCTACAGTCT
  301 TGCGCCAAAG ACGAGAAGTT GCCAAAACAT TAGCTTTGTC GAGTAATGTG ACGGGAGCAG
  361 GAGGGTTGGA AGTTTCAGCT AAGCGGCCGC GGGCCCAACC TTCCAATTCA TTAAATCGAG
  421 TCCTCCATCA GATGATTTAC AAGGTTTTGC ATAGAATCCC TAAGTAAGAG AAGTTGTTAG
  481 TGATTTCTTC AAGTTTACTA AAATTTACAT ACAAATGGAT GATCTCTTCT CCATTGTTTC
  541 CTTTCTTCTT GCAAACGCGT TTTACAAAGA GATGACATGC TATTTTCTGA TTATTTTTTT
  601 CTATAGTTTT CTATTTTGTA CGTCCTTGGG AGTCCGAAAT GTAAAATCGG GGTATGGTTA
  661 GTAGCGGCAG CGTTCATTAT GGTATATAAA AGATAAAAAG TAGGGGCAAG CGAGACAGAA
  721 TGGGATACAA GGGCATCGTC TATAATTATA GCTAAAAATT GTATTTTAAT TTGTATTTTT
  781 TGTAATTTTA TTTCTCATTG NTTTACTTAA AATGAATGCG ANTTNNAGAA AACTTCAACG
  841 AAATGTCNAA ATAAGCTCAN CAAAATATAC NNTTTTANGG AAAGCGATCA NNNACTTATT
  901 CNNCGTTGNA NANTCNTTTT CTTCNTAAAT TCCANCANNG TAACTGNCNN NTNGNNTTTT
  961 TCTCTNNNNG CTANTNAAAT TGAAACTNNN GNANNNNNAN TANCACNNGN TTTNNNNNAA
 1021 ANTNGNNNNN NNNCNTNNNT NNAAAAAANN NAANGNTNNA NNNNNAANTT NAANNNNTTN
1081 TTNNNNNNAA NNNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913. [link to RRC of NBRP]
original Matsuyama, A., A series of promoters for constitutive expression of heterologous genes in fission yeast. Yeast, 25 (5):371-376 (2008). PMID 18437702. [link to RRC of NBRP]
user_report Takayama, S., Enhancing 3-hydroxypropionic acid production in combination with sugar supply engineering by cell surface-display and metabolic engineering of Schizosaccharomyces pombe. Microb. Cell Fact. 17 (1): 176 (2018). PMID 30424766. [link to RRC of NBRP]