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pDUAL-GFH31 (#RDB06544)

S. pombe 3 ter end GFP(S65C)-FLAG-His6-tagged vector with cam1 promoter

Clone info. S. pombe 3 ter end GFP(S65C)-FLAG-His6-tagged vector with cam1 promoter
Comment Multicopy marker, ura4+; Integration marker, leu1+. Gateway incompatible.
Vector backbone pDUAL (RDB06178) (Plasmid)
Selectable markers Amp^r
Reference of insert sequence AB364132 (DDBJ accession number)
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
 
Sequence (full) RDB06544zzk03.seq provided by the depositor

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., A., Yeast, 25 (5): 371-376 (2008)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., A., Yeast, 25 (5): 371-376 (2008)) の引用と、寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06544 pDUAL-GFH31 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pDUAL-GFH31 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06544).

Reference section:

Matsuyama, A., Yoshida, M., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913. [link to RRC of NBRP]
Matsuyama, A., Shirai, A., Yoshida, M., A series of promoters for constitutive expression of heterologous genes in fission yeast. Yeast, 25 (5):371-376 (2008). PMID 18437702. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14202_A6Fnp1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: ADHrev (Pr0048)
Region: ADH1 ter,insert 3'(6xHis,FLAG,GFP(S65C)3')
Sequence file: RDB14202_A6Fne.seq check
>06544_14202_A6Fn_o_2_ADH_rev_A12_03.ab1
    1 NNNNNNNNNN NNNNNNNANN TTTCGTTTTN NACCTAAGAG TCACTTTAAA ATTTGTATAC
   61 ACTTATTTTT TTTATAACTT ATTTAATAAT AAAAATCATA AATCATAAGA AATTCGCTTA
  121 TTTAGAAGTG GCGCGCCGGA TCCTCTAGAG TCGAGATCTC AGTGGTGATG GTGGTGATGT
  181 TTGTCATCGT CGTCCTTGTA GTCCATGGGT TTGTATAGTT CATCCATGCC ATGTGTAATC
  241 CCAGCAGCTG TTACAAACTC AAGAAGGACC ATGTGGTCTC TCTTTTCGTT GGGATCTTTC
  301 GAAAGGGCAG ATTGTGTGGA CAGGTAATGG TTGTCTGGTA AAAGGACAGG GCCATCGCCA
  361 ATTGGAGTAT TTTGTTGATA ATGGTCTGCT AGTTGAACGC TTCCATCTTC AATGTTGTGT
  421 CTAATTTTGA AGTTAACTTT GATTCCATTC TTTTGTTTGT CTGCCATGAT GTATACATTG
  481 TGTGAGTTAT AGTTGTATTC CAATTTGTGT CCAAGAATGT TTCCATCTTC TTTAAAATCA
  541 ATACCTTTTA ACTCGATTCT ATTAACAAGG GTATCACCTT CAAACTTGAC TTCAGCACGT
  601 GTCTTGTAGT TCCCGTCATC TTTGAAAAAT ATAGTTCTTT CCTGTACATA GCCTTCGGGC
  661 ATGGCACTCT TGAAAAAGTC ATGCTGTTTC ATGTGATCTG GGTATCTTGA AAAGCATTGA
  721 ACACCATAAC AGAAAGTAGT GACAAGTGTT GGCCACGGAA CAGGTAGTTT TCCAGTAGTG
  781 CAAATAAATT TAANGGTAAG TTTTCCGTAT GTTGCATCAC CNTNCACCCT CTCCACNNAC
  841 AGAAAATTTG NGCCNNTNNA CNTCACCATC NNNNTCNNNN AGNNNNGGNN NNNNNNCNNN
901 NNAAAGNNCN NNNNCNTNNN NNNNNNN
//
Primer: LEU1pro_F (Pr0370)
Region: leu1 pro,Leu1 5'ORF,ura4 ter
Sequence file: RDB14202_A6Fnf.seq check
>06544_14202_A6Fn_o_2_LEU1pro_F_B12_06.ab1
    1 NNNNNNNNNA NNNNNTTGAN TTTCCCGAAA CCNGGCATTT TTGCGGTAGG GTCTGAAACT
   61 TTCACGAAAC CATTTCACAA CAAAACTTCT TCAACAACAT GTGTGCAAAG AAAATCGTCG
  121 TCTTACCAGG AGACCATATT GGCCCTGAAA TTGTTGCTTC TGCCTTGGAG GTTTTGAAAG
  181 TCGTTGAGAA GAAGCGACCT GAGTTAAAAC TCGAGTTTGA AGAACACAAG ATTGGAGGTG
  241 CCTCTATTGA TGCCTATGGA ACCCCTTTGA CTGACGAGAC TGTGAAGGCT TGTTTGGAAG
  301 CTGACGGTGT TCTTTTGGGT GCCGTTGGTG GTCCTGAATG GACCAACCCC AATTGTCGTC
  361 CTGAGCAAGG TTTATTGAAG CTTCGTAAGA GTATGGGTGT TTGGGCCAAC CTTCGACCTT
  421 GCAACTTTGC CAGCAAGTCT TTAGTCAAGT ACAGCCCTTT GAAGCCTGAA ATCGTTGAAG
  481 GTGTCGATTT TTGTGTTGTA CGAGAACTTA CTGGAGGTTG TTACTTTGGT GAGCGCACTG
  541 AGGACAACGG ATCGGGTTAT GCTATGGACA CTTGGCCTTA CAGTTTGGAA GAAGTTTCTC
  601 GTATTGCTCG TTTGGCGGCC GCGGGCCCAA CACCAATGTT TATAACCAAG TTTTATCTTG
  661 TTTGTCTACA TGGTATTTTA CATTCATCTA CATACATCTT TCATTGGCTT TGTACATAGT
  721 TATCATTACA AGTCTAAAAA AATTCACTCT TTTCTTATTC AATGTCAATC CAAGAGAAAA
  781 GANTGTGGTA ATGTTGTANG AGCATGTTTA ATAAATTACT ATAGCAAATT ACTTTTTATT
  841 CCCAAGGTGT TTATCTATAA TAGTTAATAT TTNAGTCGCT ACNTAAAATT TTACCNAAGA
  901 GTANTTGTAT ACTAATTCTA AATGCCTTCN GANATAAAAC GCCNNNNAAA ANNAANNCAA
961 ACNNNNCATC NNNTTTTTCN NNNNNNNNN
//
Primer: URA4pro_F (Pr0371)
Region: ura4 pro,ura4
Sequence file: RDB14202_A6Fng.seq check
>06544_14202_A6Fn_o_2_URA4pro_F_D12_12.ab1
    1 NNNNNNNNNN NNNNTTGTNN NNNNNNGNAC CTCTTTTTTG CTTGGNTCGA AATTAAAGGT
   61 TTAAAAGCAA AGTTATGGAT GCTAGAGTAT TTCAAAGCTA TTCAGCTAGA GCTGAGGGGA
  121 TGAAAAATCC CATTGCCAAG GAATTGTTGG CTTTGATGGA AGAAAAGCAA AGCAACTTGT
  181 CAGTCGCGGT CGATTTGACG AAGAAATCCG AAATCTTAGA ATTGGTAGAT AAAATTGGAC
  241 CCTATGTCTG TGTTATCAAG ACACATATTG ACGTTGTCGA GGATTTCGAC CAGGATATGG
  301 TAGAAAAACT GGTGGCCTTA GGTAAAAAGC ATCGTTTTCT TATCTTTGAG GATCGCAAAT
  361 TCGCAGACAT TGGAAATACC GTCAAGCTAC AATATGCATC TGGTGTGTAC AAAATTGCTT
  421 CTTGGGCTCA TATCACAAAT TGCCATACAG TGCCAGGCGA GGGTATTATA CAAGGCCTCA
  481 AAGAAGTTGG TTTACCTTTG GGACGTGGTC TCTTGCTTTT GGCTGAAATG TCTTCCAAAG
  541 GCTCTTTGGC TACTGGTTCC TACACAGAGA AAACCTTAGA ATGGTTTGAG AAGCATACCG
  601 ATTTTTGCTT TGGCTTTATA GCTGGTCGTC GATTTCCTAA CCTTCAAAGC GACTACATAA
  661 CTATGTCCCC TGGTATCGGC TTGGATGTTA AAGGAGACGG GCTGGGACAG CAATATCGTA
  721 CTCCTGAAGA AGTGATTGTA AACTGCGGTA GCGACATCAT CATTGTTGGT CGTGGAGTCT
  781 ATGGAGCTGG TCGTAATCCT GTTGTCGAAG CCNAGAGATA TAGAGAAGCT GGNTGGAANN
  841 NATATCAGCA AAGANTTTCT CAGCATTAAA AAAAGANTNA TGNAAAATTT TTTTTGGNTG
  901 GTTANTGAAA AAGTCNANGC NNTGNTNGCG TTTGTTTTCC NNNNNGNTTT ATGNCANANN
961 NNNTTTAGAN NNAGTANNNN NNTANNNTTT NGNNNAANNT TNNNNNNNNN N
//
Primer: f1ori_R3 (Pr0142)
Region: Leu1 3',ars1
Sequence file: RDB14202_A6Fnh.seq check
>06544_14202_A6Fn_o_2_f1ori_R3_C12_09.ab1
    1 NNNNNNNNNN NNNNNGCGGN NNNNATNNCG CACAGATGCG TAAGGAGAAA ATACCGCATC
   61 AGGCGCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG GGCGATCGGT GCGGGCCTCT
  121 TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA GGCGATTAAG TTGGGTAACG
  181 CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA GTGAATTCGA GCTCGGGAGC
  241 GCTACCGTGA ATGGGCTCAA CCAAACAATG AACCTTTTCT TCTGATTTTC CTACCACACC
  301 GGAAAGGGAG GCAGAAGGCA AAAGGCCCAA GCTACCAGGA ATGACAGAAG CCTCATCTGA
  361 AATAATGTCA CCAAACAAGT TGTCAGTCAA AACAACACCG TTAAGTGTAC GAGGGCTCTT
  421 GACCAAAAGC ATGGCTGCGG AGTCAATGAG CTGGTTTTTT AAGGTAAGGT GAGGATATTC
  481 CTCCTTAAAA ATCTTAGCTA CAGTCTTGCG CCAAAGACGA GAAGTTGCCA AAACATTAGC
  541 TTTGTCGAGT AATGTGACGG GAGCAGGAGG GTTGGAAGTT TCAGCTAAGC GGCCGCGGGC
  601 CCAACCTTCC AATTCATTAA ATCGAGTCCT CCATCAGATG ATTTACAAGG TTTTGCATAG
  661 AATCCCTAAG TAAGAGAAGT TGTTAGTGAT TTCTTCAAGT TTACTAAAAT TTACATACAA
  721 ATGGATGATC TCTTCTCCAT TGTTTCCTTT CTTCTTGCAA ACGCGTTTTA CAAAGAGANG
  781 ACATGCTATT TTCTGATTAT TTTTTTCTAT AGTTTTCTAT TTTGTACGTC CTTNGNANTN
  841 CGAAATGTAA AATCGGGGTA TGGNTAGTAN CGGCAGCGTT CATTATGGNA TATNAAAGAN
  901 AAAAAGTNNN GNAAGCGANA NNNAANGGGN NACNNNGNCN TCGTCTATNA NTNNAGCTAA
  961 AAANNNNANT TTNNNTNNNA NTTTTTNNAN NTTNNNTNNN NNNNNNTTNN NNNNAANNNN
1021 NNNNNN
//
Primer: cam1-pro_F (Pr1250)
Region: cam1 pro,insert 5'(GFP(S65C)5')
Sequence file: RDB14202_A6Fni.seq check
>06544_14202_B2IrF_cam1-pro_F_B06_06_ABI24.ab1
    1 NNNNNNNNNA NGNNNNNGAG CAAACNCTAA CCGCCAAAAA TAAAGGTTAA AAAAAGGANT
   61 AGAAGGACTT TAGAGTTACT GATGCTAGCG TCGACAGGCC TGGATCCGGA TATCATGAGT
  121 AAAGGAGAAG AACTTTTCAC TGGAGTTGTC CCAATTCTTG TTGAATTAGA TGGTGATGTT
  181 AATGGGCACA AATTTTCTGT CAGTGGAGAG GGTGAAGGTG ATGCAACATA CGGAAAACTT
  241 ACCCTTAAAT TTATTTGCAC TACTGGAAAA CTACCTGTTC CGTGGCCAAC ACTTGTCACT
  301 ACTTTCTGTT ATGGTGTTCA ATGCTTTTCA AGATACCCAG ATCACATGAA ACAGCATGAC
  361 TTTTTCAAGA GTGCCATGCC CGAAGGCTAT GTACAGGAAA GAACTATATT TTTCAAAGAT
  421 GACGGGAACT ACAAGACACG TGCTGAAGTC AAGTTTGAAG GTGATACCCT TGTTAATAGA
  481 ATCGAGTTAA AAGGTATTGA TTTTAAAGAA GATGGAAACA TTCTTGGACA CAAATTGGAA
  541 TACAACTATA ACTCACACAA TGTATACATC ATGGCAGACA AACAAAAGAA TGGAATCAAA
  601 GTTAACTTCA AAATTAGACA CAACATTGAA GATGGAAGCG TTCAACTAGC AGACCATTAT
  661 CAACAAAATA CTCCAATTGG CGATGGCCCT GTCCTTTTAC CAGACAACCA TTACCTGTCC
  721 ACACAATCTG CCCTTTCGAA AGATCCCAAC GAAAAGAGAG ACCACATGGT CCTTCTTGAG
  781 TTTGTAACAG CTGCTGGGAN TACACATGGC ATGGATGAAC TATACAAACC CATGGACTAC
  841 NANGACGANG ATGACAAACA TCACCACCAT CACCACTGAN NTCTCGACTC TAGANGANCC
  901 GGCGCGCCNC TTCTNAATNA NCGAANTTCT NANGANTNNT GANTTTTNTT ATTNAATAAN
  961 TTATAAAAAA AANTAAGTGN NNANNANTTT TAAAGTGNNN NNNNNNTTTN AAAANNAAAA
1021 NTNNNNNNNN NN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913. [link to RRC of NBRP]
original Matsuyama, A., A series of promoters for constitutive expression of heterologous genes in fission yeast. Yeast, 25 (5):371-376 (2008). PMID 18437702. [link to RRC of NBRP]