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pARG1H (#RDB06558)

S. pombe vector for chromosomal integration

Clone info. S. pombe vector for chromosomal integration
Comment For detail, please refer Figs. 1 and 2 of Matsuyama, A., Biochem. Biophys. Res. Commun. 374, 315-319, 2008.
Vector backbone pUC119 (Plasmid)
Selectable markers Amp^r
Reference of insert sequence AB364159.1 (DDBJ accession number)
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
 
Sequence RDB06558z.seq

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Biochem. Biophys. Res. Commun. 374, 315-319, 2008) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Biochem. Biophys. Res. Commun. 374, 315-319, 2008)の引用と、寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06558 pARG1H DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pARG1H was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06558).

Reference section:

Matsuyama, A., Yoshida, M., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913. [link to RRC of NBRP]
Matsuyama, A,, Shirai, A,, Yoshida, M., A novel series of vectors for chromosomal integration in fission yeast. Biochem. Biophys. Res. Commun. 374 (2): 315-319 (2008). PMID 18634753. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14295_A6Hhp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: TEFpro_F
Sequence file: RDB14295_A6Hha.seq check
>06558_14295_A6Hh_TEFpro_F_D01_10_ABI24.ab1
    1 TCCAGCGCGA GCAAATTACG GCTCCTCGCT GCGGACCTGC GAGCAGGGAA ACGCTCCCCT
   61 CACAGACGCG TTGAATTGTC CCCACGCCGC GCCCCTGTAG AGAAATATAA AAGGTTAGGA
  121 TTTGCCACTG AGGTTCTTCT TTCATATACT TCCTTTTAAA ATCTTGCTAG GATACAGTTC
  181 TCACATCACA TCCGAACATA AACAACCATG GGTAAAAAGC CTGAACTCAC CGCGACGTCT
  241 GTCGAGAAGT TTCTGATCGA AAAGTTCGAC AGCGTCTCCG ACCTGATGCA GCTCTCGGAG
  301 GGCGAAGAAT CTCGTGCTTT CAGCTTCGAT GTAGGAGGGC GTGGATATGT CCTGCGGGTA
  361 AATAGCTGCG CCGATGGTTT CTACAAAGAT CGTTATGTTT ATCGGCACTT TGCATCGGCC
  421 GCGCTCCCGA TTCCGGAAGT GCTTGACATT GGGGAATTCA GCGAGAGCCT GACCTATTGC
  481 ATCTCCCGCC GTGCACAGGG TGTCACGTTG CAAGACCTGC CTGAAACCGA ACTGCCCGCT
  541 GTTCTGCAGC CGGTCGCGGA GGCCATGGAT GCGATCGCTG CGGCCGATCT TAGCCAGACG
  601 AGCGGGTTCG GCCCATTCGG ACCGCAAGGA ATCGGTCAAT ACACTACATG GCGTGATTTC
  661 ATATGCGCGA TTGCTGATCC CCATGTGTAT CACTGGCAAA CTGTGATGGA CGACACCGTC
  721 AGTGCGTCCG TCGCGCAGGC TCTCGATGAG CTGATGCTTT GGGCCGAGGA CTGCCCCGAA
  781 GTCCGGCACC TCGTGCACGC GGATTTCGGC TCCAACAATG TCCTGACGGA CAATGGCCGC
  841 ATAACAGCGG TCATTGACTG GAGCGAGGCG ATGTTCGGGG GATTCCCAAT ACGAGGTCGC
  901 CAACATCTTC TTCTGGAGGC CGTGGTTGGC TTGTATGGAG CAGCAGACGC GCTACTTCGA
  961 GCGGAGGCAT CCGGGAGCTT GCAGGATCGC CGCGGCTCCC GGGCGTATAT GCTCCCGCAT
 1021 TGGGTCTTGA CCCAACTCTA TCAGAGCTTT GCATGGACGG CAATTTCGAT GAATGCAGCC
1081 TGGGCGCAAG GTTCGATGCG ACGCCAATTC GATTCCGGA
//
Primer: TEFter_R
Sequence file: RDB14295_A6Hhb.seq check
>06558_14295_A6Hh_TEFter_R_B01_04_ABI24.ab1
    1 AACAAGGCTA CATTTTGATT AAAATAGAAC AACTACAATA TAAAAAAACT ATACAAATGA
   61 CAAGTTCTTG AAAACAAGAA TCTTTTTATT GTCAGTACTG ATTATTCCTT TGCCCTCGGA
  121 CGAGTGCTGG GGCGTCGGTT TCCACTATCG GCGAGTACTT CTACACAGCC ATCGGTCCAG
  181 ACGGCCGCGC TTCTGCGGGC GATTTGTGTA CGCCCGACAG TCCCGGCTCC GGATCGGACG
  241 ATTGCGTCGC ATCGACCCTG CGCCCAAGCT GCATCATCGA AATTGCCGTC AACCAAGCTC
  301 TGATAGAGTT GGTCAAGACC AATGCGGAGC ATATACGCCC GGAGCCGCGG CGATCCTGCA
  361 AGCTCCGGAT GCCTCCGCTC GAAGTAGCGC GTCTGCTGCT CCATACAAGC CAACCACGGC
  421 CTCCAGAAGA AGATGTTGGC GACCTCGTAT TGGGAATCCC CGAACATCGC CTCGCTCCAG
  481 TCAATGACCG CTGTTATGCG GCCATTGTCC GTCAGGACAT TGTTGGAGCC GAAATCCGCG
  541 TGCACGAGGT GCCGGACTTC GGGGCAGTCC TCGGCCCAAA GCATCAGCTC ATCGAGAGCC
  601 TGCGCGACGG ACGCACTGAC GGTGTCGTCC ATCACAGTTT GCCAGTGATA CACATGGGGA
  661 TCAGCAATCG CGCATATGAA ATCACGCCAT GTAGTGTATT GACCGATTCC TTGCGGTCCG
  721 AATGGGCCGA ACCCGCTCGT CTGGCTAAGA TCGGCCGCAG CGATCGCATC CATGGCCTCC
  781 GCGACCGGCT GCAGAACAGC GGGCAGTTCG GTTTCAGGCA GGTCTTGCAA CGTGACACCC
  841 TGTGCACGGC GGGAGATGCA ATAGGTCACG CTCTCGCTGA ATTCCCCAAT GTCAAGCACT
  901 TCCGGAAATC GGGAGCGCGG CCGATGCAAA GTGCCGATAA ACATAACGAT CTTTGTAGAA
  961 ACCATCGGCG CAGCTATTTA CCCGCAGGAC ATATCCACGC CCTCCTACAT CGAAAGCTGA
 1021 AAGCACGAGA TTCTTCGCCC TCGAGAGCTG CATCAGGTCG GAGACGCTGG TCGGACTTTT
1081 TCGATCAGAA AGATTCCTCC GAC
//
Primer: ADH-Forward
Sequence file: RDB14295_A6Hhc.seq check
>06558_14295_A6Hh_ADH_Forward_F01_16_ABI24.ab1
    1 TACTCCTATT GACACACCTC TACCGGCAGA TCCGCTAGGG ATAACAGGGT AATATACTAG
   61 TCGTAGCTGC TAAAGGTGAG GGCTCCTATT TGTTTGACAA GGAAGGTCGT AAATACATTG
  121 ACTTCACCTC TGGCGTAGCT GTTACATCTT TGGGTCATGC TCATCCTGAA GTTGCTAGAC
  181 TTGCCGCTGA TCAATGCTCC AAATTAGTCC ATAGTAGCAA CCTTTTCTAC AACGAGCCTG
  241 CCATTGAGCT ATCTAATGTC ATTAATAATT CTTTGGCAAA AAATAGCGGT ATTGCTGGCC
  301 CCACAAAAAT TTTCTTTGCC AATTGTGGTA CTGAGGCCAA TGAGACAGCC TTGAAGTTCG
  361 CTCGTAAGGC GGCCGCGGGC CCGCGGCCGC GTTTGAGAAG TATGGAGAAG GGAAGAGTCA
  421 AATTGTTTAC TTTAATAACT CTTTCCACGG CCGTTCTTTG GGATCTCTTA GTATCACTGC
  481 GAACCCCAAA TATAAACGTG GTTTCCAACC ATTACTTCCT GATGTCGTCC AAGCCGTCTA
  541 TAATGACCCT GCATCCATAG AGCAATTTGT CAATGACAAA ACTGCGGCTG TTATTGTCGA
  601 GCCCGTTCAA GGTGAAGGTG GTATTTGTCC TGCCAAGCCT GAATTTTTGA TTGCTTTGCG
  661 CAAGGCCTGT GATAAGGTTG GTGCTTCTTT AATCTATGAT GAAATTCAAT GCGGACTTGG
  721 TCGCAGATCT GTTTAGCTTG CCTCGTCCCC GCCGGGTCAC CCGGCCAGCG ACATGGAGGC
  781 CCAGAATACC CTCCTTGACA GTCTTGACGT GCGCAGCTCA GGGCATGATG TGACTGTCGC
  841 CCGTACATTT AGCCCATACA TCCCCATGTA TAATCATTTG CATCCATACA TTTTGATGGC
  901 CGCACGGCGC GAAGCAAAAA ATTACGGCTC CTCGCTGCGG ACCTGCGAGC AGGGAAACGC
  961 TCCCCTCACA GACGCGTTTG AATTGTCCCC ACGCCGCGCC CCTGTAGAGA ATATAAAAGG
 1021 TTAAGATTTG CACTGAGTTC TCTTTCATAT ACTCTTTTAA AATCTTGCTA GATACAGCTC
1081 TCACATCACA TCCGCAACAT ACATCATGTT CAAAGGCCG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913. [link to RRC of NBRP]
original Matsuyama, A,, A novel series of vectors for chromosomal integration in fission yeast. Biochem. Biophys. Res. Commun. 374 (2): 315-319 (2008). PMID 18634753. [link to RRC of NBRP]