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pLYS1U-Pnmt1 (#RDB06567)

S. pombe expression vector with nmt1 promoter

Clone info. S. pombe expression vector with nmt1 promoter
Vector backbone pUC119 (Plasmid)
Selectable markers Amp^r
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
 
Sequence RDB06567z.seq

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Methods Mol. Biol. 824: 433-450 (2012)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Methods Mol. Biol. 824: 433-450 (2012)) の引用と、寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06567 pLYS1U-Pnmt1 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pLYS1U-Pnmt1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06567).

Reference section:

Matsuyama, A., Yoshida, M., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB06567_A4Kj.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40
Sequence file: RDB06567_A4Kja.seq check
>06567_06567_A4Kj_M13(-40)_A07_01.ab1
    1 GCGGGCAGGG ATTCGAGCTC GTTTAACGTG ATTTTTATCT TGTTTGTCTA CATGGTATTT
   61 TACATTCATC TACATACATC TTTCATTGGC TTTGTACATA GTTATCATTA CAAGTCTAAA
  121 AAAATTCACT CTTTTCTTAT TCAATGTCAA TCCAAGAGAA AAGATTGTGG TAATGTTGTA
  181 GGAGCATGTT TAATAAATTA CTATAGCAAA TTACTTTTTA TTCCCAAGGT GTTTATCTAT
  241 AATAGTTAAT ATTTTAGTCG CTACATAAAA TTTTACCAAA GAGTACTTGT ATACTAATTC
  301 TAAATGCCTT CTGACATAAA ACGCCTAGGA AAACAAACGC AAACAAGGCA TCGACTTTTT
  361 CAATAACCAA CCAAAAAAAT TTTACATTAG TCTTTTTTTA ATGCTGAGAA AGTCTTTGCT
  421 GATATGCCTT CCAACCAGCT TCTCTATATC TCTTGGCTTC GACAACAGGA TTACGACCAG
  481 CTCCATAGAC TCCACGACCA ACAATGATGA TATCGCTACC GCAGTTTACA ATCACTTCTT
  541 CAGGAGTACG ATATTGCTGT CCCAGCCCGT CTCCTTTAAC ATCCAAGCCG ATACCAGGGG
  601 ACATAGTTAT GTAGTCGCTT TGAAGGTTAG GAAATCGACG ACCAGCTATA AAGCCAAAGC
  661 AAAAATCGGT ATGCTTCTCA AACCATTCTA AGGTTTTCTC TGTGTAGGAA CCAGTAGCCA
  721 AAGAGCCTTT GGAAGACATT TCAGCCAAAA GCAAGAGACC ACGTCCCAAA GGTAAACCAA
  781 CTTCTTTGAG GCCTTGTATA ATACCCTCGC CTGGCACTGT ATGGCAATTT GTGATATGAG
  841 CCCAAGAAGC AATTTTGTAC ACACCAGATG CATATTGTAG CTTGACGGTA TTTCCAATGT
  901 CTGCGAATTT GCGATCCTCA AAGATAAGAA AACGATGCTT TTTACCTAAA GGCCACCAGT
  961 TTTTCTACCA TATCCTGGTC GAAATCCTCG ACAACGTCAA TATGTGTCTT GATAACACAG
 1021 ACATAGGGTC CAATTTTATC TACCAAATTC TAAGATTTCG GATTTCTTCG TCAATCGACC
 1081 GCGACTGACA GTGCTTTGCT TTTTCTTCAT CAAGCCAACA ATCTGCCATT GGAATTTTTC
1141 ATCCCCCTCA GCTTCTAGCC TGGAAATATA GAGCT
//
Primer: nmt1Fw
Sequence file: RDB06567_A4Kjb.seq check
>06567_06567_A4Kj_nmt1Fw_B07_04.ab1
    1 GGGATTTAAT TAATCAGGGA AAACGTAACT CTCGGCTACT GGATGGTTCA GTCACCCAAC
   61 GATTACTGGG GAGAGAAAAC AGGGCAAAAG CAAAGCTTAA AGGAATCCGA TTGTCATTCG
  121 GCAATGTGCA GCGAAACTAA AAACCGGATA ATGGACCTGT TAATCGAAAC ATTGAAGATA
  181 TATAAAGGAA GAGGAATCCT GGCATATCAT CAATTGAATA AGTTGAATTA ATTATTTCAA
  241 TCTCATTCTC ACTTTCTGAC TTATAGTCGC TTTGTTAAAG CTAGCGTCGA CAGGCCTGGA
  301 TCCGGCGCGC CACTTCTAAA TAAGCGAATT TCTTATGATT TATGATTTTT ATTATTAAAT
  361 AAGTTATAAA AAAAATAAGT GTATACAAAT TTTAAAGTGA CTCTTAGGTT TTAAAACGAA
  421 AATTCTTATT CTTGAGTAAC TCTTTCCTGT AGGTCAGGTT GCTTTCTCAG GTATAGTATG
  481 AGGTCGCTCT TATTGACCAC ACCTCTACCG GCAGATCCGC TAGGGATAAC AGGGTAATAT
  541 ACTAGTACTT TGTAGTACCT GCGCGGTCTC AAGATCAAAC ATTTTTGGAA TCGCAAAAAG
  601 ATGTCATTCC AGCCGGACGT GGCATGAAGA ATGTCCAACT TTTGGTGATC AATCGTTTTG
  661 ATACTAACAA GATATGCGGT ATCGGAGAAG TTGGTGAAAT ATATCTTCGT GCTGGTGGAT
  721 TGGCTGAGGG CTACCTTGGA AATGATGAAT TGACTAGCAA AAAATTCTTA AAGAGTTGGT
  781 TTGCTGATCC CTCCAAGTTT GTAGACCGAA CTCCAGAAAA TGCGCCCTGG AAACCTTATT
  841 GGTTCGGAAT ACGCGATCGT ATGTATCGTT CTGGTGACTT GGGCCGCTAT CTCCCAACTG
  901 GTAATGTAGA AATGTAGCGG CCGCGGGCCC GCGGCCGCCG ACGATCAAAT TAAAATTCGT
  961 GGTTTCCGAA TTGAGTTAGG TGAAATTAAT ACCCATCTTT CTCGTCATCC TAATGTCCGT
 1021 GAAAATATTA CCCTTTGTTC GACGTGATAA GGATGAGAAC CCACGCTAGT TGCATACATT
 1081 GTACCCTCAA GTTAATAAAG ATGACTTTGA TAGCGCTTAC TGAGTCCGAG GATATTGGTT
1141 CGTTCATGGA TTGAGAGTAA CAAGGAAGCT TTTTATT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913. [link to RRC of NBRP]