icon below as well as clone information before placing your order.
S. pombe expression vector with nmt1 promoter
|
Other clones in our bank
External Database Reference sequence |
| Sequence | RDB06567z.seq |
|---|
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Methods Mol. Biol. 824: 433-450 (2012)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR . When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation. |
|---|---|
| Ordering | Order form [Credit Card Payment  ] [Bank Transfer Payment]Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word ]Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. |
[open/close]| 提供条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Methods Mol. Biol. 824: 433-450 (2012)) の引用と、寄託者への謝辞の表明を必要とする。 利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。 寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。 |
|---|---|
| 提供依頼 | 提供依頼書 [Word ]提供同意書 (MTA、非営利機関による非営利学術研究用)[Word ]手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB06567 | pLYS1U-Pnmt1 | DNA solution |
icon below as well as clone information before placing your order.Materials & Methods section:
| The pLYS1U-Pnmt1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06567). |
Reference section:
| Matsuyama, A., Yoshida, M., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Featured content
| Featured content | Expression Vector Backbone of Schizosaccharomyces pombe (English text) |
|---|
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
| Test sheet | RDB06567_A4Kj.pdf |
|---|
Nucleotide sequence of a portion of this resource (if available).
|
Primer: M13_-40 Sequence file: RDB06567_A4Kja.seq
|
|---|
|
>06567_06567_A4Kj_M13(-40)_A07_01.ab1 1 GCGGGCAGGG ATTCGAGCTC GTTTAACGTG ATTTTTATCT TGTTTGTCTA CATGGTATTT 61 TACATTCATC TACATACATC TTTCATTGGC TTTGTACATA GTTATCATTA CAAGTCTAAA 121 AAAATTCACT CTTTTCTTAT TCAATGTCAA TCCAAGAGAA AAGATTGTGG TAATGTTGTA 181 GGAGCATGTT TAATAAATTA CTATAGCAAA TTACTTTTTA TTCCCAAGGT GTTTATCTAT 241 AATAGTTAAT ATTTTAGTCG CTACATAAAA TTTTACCAAA GAGTACTTGT ATACTAATTC 301 TAAATGCCTT CTGACATAAA ACGCCTAGGA AAACAAACGC AAACAAGGCA TCGACTTTTT 361 CAATAACCAA CCAAAAAAAT TTTACATTAG TCTTTTTTTA ATGCTGAGAA AGTCTTTGCT 421 GATATGCCTT CCAACCAGCT TCTCTATATC TCTTGGCTTC GACAACAGGA TTACGACCAG 481 CTCCATAGAC TCCACGACCA ACAATGATGA TATCGCTACC GCAGTTTACA ATCACTTCTT 541 CAGGAGTACG ATATTGCTGT CCCAGCCCGT CTCCTTTAAC ATCCAAGCCG ATACCAGGGG 601 ACATAGTTAT GTAGTCGCTT TGAAGGTTAG GAAATCGACG ACCAGCTATA AAGCCAAAGC 661 AAAAATCGGT ATGCTTCTCA AACCATTCTA AGGTTTTCTC TGTGTAGGAA CCAGTAGCCA 721 AAGAGCCTTT GGAAGACATT TCAGCCAAAA GCAAGAGACC ACGTCCCAAA GGTAAACCAA 781 CTTCTTTGAG GCCTTGTATA ATACCCTCGC CTGGCACTGT ATGGCAATTT GTGATATGAG 841 CCCAAGAAGC AATTTTGTAC ACACCAGATG CATATTGTAG CTTGACGGTA TTTCCAATGT 901 CTGCGAATTT GCGATCCTCA AAGATAAGAA AACGATGCTT TTTACCTAAA GGCCACCAGT 961 TTTTCTACCA TATCCTGGTC GAAATCCTCG ACAACGTCAA TATGTGTCTT GATAACACAG 1021 ACATAGGGTC CAATTTTATC TACCAAATTC TAAGATTTCG GATTTCTTCG TCAATCGACC 1081 GCGACTGACA GTGCTTTGCT TTTTCTTCAT CAAGCCAACA ATCTGCCATT GGAATTTTTC 1141 ATCCCCCTCA GCTTCTAGCC TGGAAATATA GAGCT // |
|
Primer: nmt1Fw Sequence file: RDB06567_A4Kjb.seq
|
|
>06567_06567_A4Kj_nmt1Fw_B07_04.ab1 1 GGGATTTAAT TAATCAGGGA AAACGTAACT CTCGGCTACT GGATGGTTCA GTCACCCAAC 61 GATTACTGGG GAGAGAAAAC AGGGCAAAAG CAAAGCTTAA AGGAATCCGA TTGTCATTCG 121 GCAATGTGCA GCGAAACTAA AAACCGGATA ATGGACCTGT TAATCGAAAC ATTGAAGATA 181 TATAAAGGAA GAGGAATCCT GGCATATCAT CAATTGAATA AGTTGAATTA ATTATTTCAA 241 TCTCATTCTC ACTTTCTGAC TTATAGTCGC TTTGTTAAAG CTAGCGTCGA CAGGCCTGGA 301 TCCGGCGCGC CACTTCTAAA TAAGCGAATT TCTTATGATT TATGATTTTT ATTATTAAAT 361 AAGTTATAAA AAAAATAAGT GTATACAAAT TTTAAAGTGA CTCTTAGGTT TTAAAACGAA 421 AATTCTTATT CTTGAGTAAC TCTTTCCTGT AGGTCAGGTT GCTTTCTCAG GTATAGTATG 481 AGGTCGCTCT TATTGACCAC ACCTCTACCG GCAGATCCGC TAGGGATAAC AGGGTAATAT 541 ACTAGTACTT TGTAGTACCT GCGCGGTCTC AAGATCAAAC ATTTTTGGAA TCGCAAAAAG 601 ATGTCATTCC AGCCGGACGT GGCATGAAGA ATGTCCAACT TTTGGTGATC AATCGTTTTG 661 ATACTAACAA GATATGCGGT ATCGGAGAAG TTGGTGAAAT ATATCTTCGT GCTGGTGGAT 721 TGGCTGAGGG CTACCTTGGA AATGATGAAT TGACTAGCAA AAAATTCTTA AAGAGTTGGT 781 TTGCTGATCC CTCCAAGTTT GTAGACCGAA CTCCAGAAAA TGCGCCCTGG AAACCTTATT 841 GGTTCGGAAT ACGCGATCGT ATGTATCGTT CTGGTGACTT GGGCCGCTAT CTCCCAACTG 901 GTAATGTAGA AATGTAGCGG CCGCGGGCCC GCGGCCGCCG ACGATCAAAT TAAAATTCGT 961 GGTTTCCGAA TTGAGTTAGG TGAAATTAAT ACCCATCTTT CTCGTCATCC TAATGTCCGT 1021 GAAAATATTA CCCTTTGTTC GACGTGATAA GGATGAGAAC CCACGCTAGT TGCATACATT 1081 GTACCCTCAA GTTAATAAAG ATGACTTTGA TAGCGCTTAC TGAGTCCGAG GATATTGGTT 1141 CGTTCATGGA TTGAGAGTAA CAAGGAAGCT TTTTATT // |
Please visit 
Sequencing and PCR primers for primer information.
Original, user report and related articles
| original | Matsuyama, A., Heterologous gene expression by chromosomal integration in fission yeast. Methods Mol. Biol. 824: 433-450 (2012). PMID 22160913. [link to RRC of NBRP] |
|---|
2023.08.18
GNP_filter3_RDBDEP_html_230702.pl