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pFFH41 (#RDB06587)

S. pombe expression vector with nmt1 promoter

Clone info. S. pombe expression vector with nmt1 promoter
Vector backbone pREP41 (Plasmid)
Selectable markers Amp^r
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
 
Sequence RDB06587z.seq

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、　寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06587 pFFH41 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pFFH41 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06587).

Reference section:

Matsuyama, A., Shirai, A., Yashiroda, Y., Kamata, A., Horinouchi, S., Yoshida, M., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14498_A6L7p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw
Sequence file: RDB14498_A6L7a.seq check
>06587_14498_A6L7_nmt1Fw_A10_01_ABI24.ab1
    1 TGGAATTTAA TTAAATCAGG AAAACGTAAC TCTCGGCTAC TGGATGGTTC AGTCACCCAA
   61 CGATTACTGG GGAGAGAAAA CAGGGCAAAA GCAAAGCTTA AAGGAATCCG ATTGTCATTC
  121 GGCAATGTGC AGCGAAACTA AAAACCGGAT AATGGACCTG TTAATCGAAA CATTGAAGAT
  181 AAAGGAAGAG GAATCCTGGC ATATCATCAA TTGAATAAGT TGAATTAATT ATTTCAATCT
  241 CATTCTCACT TTCTGACTTA TAGTCGCTTT GTTAAAGCTA GCGTCGACAG GCCTGGATCC
  301 GGATATCATG GATTATAAGG ATGATGACGA TAAACCCATG GACTACAAGG ACGACGATGA
  361 CAAACATCAC CACCATCACC ACTGAGATCT CGAGCCCGGG CGGCCGCTAA CTAACTAAGG
  421 GTAAAAGGAA TGTCTCCCTT GCCAGTACTG CTAGGGTTTT TCTTTCAAAC TATGGAAGCC
  481 CATTCAAGCT GCATATTACG ATTTTGTTTT TCGCTTTTAG AAAGTGGTTT AGATGAGATA
  541 ATAGAAAAAT TCTTGATCTC CGACAACGAG TACTTTTATT TTTTTTGCTA ATCACTTTAC
  601 TCAATATTAG CTCGAAATCG TAGAAACGTA GACGGGTGCG GGATACCGAG TGGTGTAGTT
  661 AAGAATTTTT ATAAACCACG TGGCCCAAAA ATATGAACCC AAAACGTTTA TACATGAGTA
  721 TACTTTAAGA AGGCTATACC CCTTCGTGTT AGATGTAGTT TTAGCTACCC AACCCGAGTC
  781 TATGAGCTTG ACTTCAGATG TAGAAGGCAT TAAATCGTTT TGAATATTAA TTAAAAAACG
  841 ATGAAAATTA AATATTTAAA AGCAATCATA CGCTGAAAAT TTAGTGCTGT GGCTAATCCT
  901 TCAACATGGA AATGCCATAA AAGTGACTTT GACAAAAAAA AAAAAGTATA TACAGGGTAG
  961 TAAACTCATC TACTTCATTT GACTTTGTTT ACAGCATGTG GAAGGAAGAA TATTTATTTG
 1021 CTAAATTCGT TAGTTTTACC ATTTCCAATA AGTAATACCT AATGAAATCC GACAGATTGG
1081 CGCATGATGA AAAGAGCCAT TTTGCTTTTG GGGAAGAAAC TG
//
Primer: f1ori_R3
Sequence file: RDB14498_A6L7b.seq check
>06587_14498_A6L7_f1ori_R3_C10_07_ABI24.ab1
    1 GCCAGAGGGG CGTTGAGTAT GCGGGTGTGA ATACCGCACA GATGCGTAAG GAGAAAATAC
   61 CGCATCAGGC GCCATTCGCC ATTCAGGCTG CGCAACTGTT GGGAAGGGCG ATCGGTGCGG
  121 GCCTCTTCGC TATTACGCCA GCTGGCGAAA GGGGGATGTG CTGCAAGGCG ATTAAGTTGG
  181 GTAACGCCAG GGTTTTCCCA GTCACGACGT TGTAAAACGA CGGCCAGTGA ATTCCAACCT
  241 TCCAATTCAT TAAATCGAGT CCTCCATCAG ATGATTTACA AGGTTTTGCA TAGAATCCCT
  301 AAGTAAGAGA AGTTGTTAGT GATTTCTTCA AGTTTACTAA AATTTACATA CAAATGGATG
  361 ATCTCTTCTC CATTGTTTCC TTTCTTCTTG CAAACGCGTT TTACAAAGAG ATGACATGCT
  421 ATTTTCTGAT TATTTTTTTC TATAGTTTTC TATTTTGTAC GTCCTTGGGA GTCCGAAATG
  481 TAAAATCGGG GTATGGTTAG TAGCGGCAGC GTTCATTATG GTATATAAAA GATAAAAAGT
  541 AGGGGCAAGC GAGACAGAAT GGGATACAAG GGCATCGTCT ATAATTATAG CTAAAAATTG
  601 TATTTTAATT TGTATTTTTT GTAATTTTAT TTCTCATTGT TTTACTTAAA ATGAATGCGA
  661 ATTAGAGAAA ACTTCAACGA AATGTCAAAA TAAGCTCAGC AAAATATACA ATTTTAGGGA
  721 AAGCGATCAG CAACTTATTC TCCGTTGTAG ATTCTTTTTC TTCATAAATT CCAACAATGT
  781 AACTGCCAAT TGATTTTTCT CTAGATGCTA ATAAATTGAA ACTAATGAAT ATAATAACAC
  841 AGGTTTTGAT AAATTGTAAA CTCTTACTCA AAAAAAAGAA TGTTAAAAAC TAATTTAACC
  901 TTTTTATAAA GGCTCATGGG CGTTTAAAGA GGATAGTAAA AATTCTACAA TTTTTTAATT
  961 TAAATGTCAC AATTGTCTTG CGAATATAAT TCTAATTAAT TTTTCGCTTA ACTGCTTGTT
 1021 TAGTACTATA CTTTCAATAT GCGTTATATG CAACCTTTAT CCATGGAGAT TGCGATATTT
1081 TTGGCAATCT ATCCTGGTAC CTTGGGAGGC GGCGTTCTAA CTCAAC
//
Primer: LEU2pro_F
Sequence file: RDB14498_A6L7c.seq check
>06587_14498_A6L7_LEU2pro_F_B10_04_ABI24.ab1
    1 AATCCGGCAG TTGAATATTA TTTAAGGACC TATTGTTTTT TCCAATAGGT GGTTAGCAAT
   61 CGTCTTACTT TCTAACTTTT CTTACCTTTT ACATTTCAGC AATATATATA TATATTTCAA
  121 GGATATACCA TTCTAATGTC TGCCCCTAAG AAGATCGTCG TTTTGCCAGG TGACCACGTT
  181 GGTCAAGAAA TCACAGCCGA AGCCATTAAG GTTCTTAAAG CTATTTCTGA TGTTCGTTCC
  241 AATGTCAAGT TCGATTTCGA AAATCATTTA ATTGGTGGTG CTGCTATCGA TGCTACAGGT
  301 GTTCCACTTC CAGATGAGGC GCTGGAAGCC TCCAAGAAGG CTGATGCCGT TTTGTTAGGT
  361 GCTGTGGGTG GTCCTAAATG GGGTACCGGT AGTGTTAGAC CTGAACAAGG TTTACTAAAA
  421 ATCCGTAAAG AACTTCAATT GTACGCCAAC TTAAGACCAT GTAACTTTGC ATCCGACTCT
  481 CTTTTAGACT TATCTCCAAT CAAGCCACAA TTTGCTAAAG GTACTGACTT CGTTGTTGTC
  541 AGAGAATTAG TGGGAGGTAT TTACTTTGGT AAGAGAAAGG AAGACGATGG TGATGGTGTC
  601 GCTTGGGATA GTGAACAATA CACCGTTCCA GAAGTGCAAA GAATCACAAG AATGGCCGCT
  661 TTCATGGCCC TACAACATGA GCCACCATTG CCTATTTGGT CCTTGGATAA AGCTAATGTT
  721 TTGGCCTCTT CAAGATTATG GAGAAAAACT GTGGAGGAAA CCATCAAGAA CGAATTCCCT
  781 ACATTGAAGG TTCAACATCA ATTGATTGAT TCTGCCGCCA TGATCCTAGT TAAGAACCCA
  841 ACCCACCTAA ATGGTATTAT AATCACCAGC AACATGTTTG GTGACATCAT CTCCGATGAA
  901 GCCCTCCGTT ATCCCAGGTT TCCTTGGGTT TGTTGCCATC TGCGTCCTTG GCCTCTTTGC
  961 CAGACAAGAA CACCGCATTT GGTTTGTACG AACCCATGCA CGTTCTGCTC CAGATTTGCC
 1021 AAAGATAAGT CCACCCTATC GCACTATCTG GTCTGCCTGC ATGAATGTGA ATGTCATTGA
1081 ACTTGCTGGA GAGGTAAGGC ATTGG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162. [link to RRC of NBRP]