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pFFH81 (#RDB06588)

S. pombe expression vector with nmt1 promoter

Clone info. S. pombe expression vector with nmt1 promoter
Vector backbone pREP81 (Plasmid)
Selectable markers Amp^r
Depositor|Developer Yoshida, Minoru | Matsuyama, Akihisa |
 
Sequence RDB06588z.seq

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) and state an acknowledgement to the DEPOSITOR. When the RECIPIENT wishes to sell the BIOLOGICAL RESOURCE and/or its DERIVATIVES on commercial basis, the RECIPIENT must obtain a prior written permission from the DEPOSITOR. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Matsuyama, A., Yeast 21 (15):1289-1305 (2004)) の引用と、　寄託者への謝辞の表明を必要とする。　利用者が本件リソース又はその派生物の販売を望む場合、書面により、寄託者の事前の承認を得る。　寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06588 pFFH81 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pFFH81 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06588).

Reference section:

Matsuyama, A., Shirai, A., Yashiroda, Y., Kamata, A., Horinouchi, S., Yoshida, M., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14499_A6L7p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: nmt1Fw
Sequence file: RDB14499_A6L7a.seq check
>06588_14499_A6L7_nmt1Fw_D10_10_ABI24.ab1
    1 ATAATTTTTA TTAAATCAGG AAAAACGTAA CTCTCGGCTA CTGGATGGTT CAGTCACCCA
   61 ACGATTACTG GGGAGAGAAA ACAGGGCAAA AGCAAAGCTT AAAGGAATCC GATTGTCATT
  121 CGGCAATGTG CAGCGAAACT AAAAACCGGA TAATGGACCT GTTAATCGAA ACATTGAAGA
  181 TGGAAGAGGA ATCCTGGCAT ATCATCAATT GAATAAGTTG AATTAATTAT TTCAATCTCA
  241 TTCTCACTTT CTGACTTATA GTCGCTTTGT TAAAGCTAGC GTCGACAGGC CTGGATCCGG
  301 ATATCATGGA TTATAAGGAT GATGACGATA AACCCATGGA CTACAAGGAC GACGATGACA
  361 AACATCACCA CCATCACCAC TGAGATCTCG AGCCCGGGCG GCCGCTAACT AACTAAGGGT
  421 AAAAGGAATG TCTCCCTTGC CAGTACTGCT AGGGTTTTTC TTTCAAACTA TGGAAGCCCA
  481 TTCAAGCTGC ATATTACGAT TTTGTTTTTC GCTTTTAGAA AGTGGTTTAG ATGAGATAAT
  541 AGAAAAATTC TTGATCTCCG ACAACGAGTA CTTTTATTTT TTTTGCTAAT CACTTTACTC
  601 AATATTAGCT CGAAATCGTA GAAACGTAGA CGGGTGCGGG ATACCGAGTG GTGTAGTTAA
  661 GAATTTTTAT AAACCACGTG GCCCAAAAAT ATGAACCCAA AACGTTTATA CATGAGTATA
  721 CTTTAAGAAG GCTATACCCC TTCGTGTTAG ATGTAGTTTT AGCTACCCAA CCCGAGTCTA
  781 TGAGCTTGAC TTCAGATGTA GAAGGCATTA AATCGTTTTG AATATTAATT AAAAAAACGA
  841 TGAAAATTAA ATATTTAAAA GCAATCATAC GCTGAAAATT TAGTGCTGTG GCTAATCCTT
  901 CAACATGGAA ATGCCATAAA AGTGACTTTG ACAAAAAAAA AAAAGTATAT ACAGGTAGTA
  961 AACTCATCTA CTTCATTGAC TTTGTTTACA GCATGTGAAG GAGATATTAT TTGCTAATCG
 1021 TAGTTACATT CAATAAGTAT ACTATTGGAA ATCCGACAGA TGGTCGCATG CATGAAGAGG
1081 CCATTTTGCC TTTGGGGGAG AATTAGCTTC
//
Primer: f1ori_R3
Sequence file: RDB14499_A6L7b.seq check
>06588_14499_A6L7_f1ori_R3_F10_16_ABI24.ab1
    1 GCAGTGCTGC TTGCGTATGC GGTGTGAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC
   61 ATCAGGCGCC ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC
  121 TCTTCGCTAT TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA
  181 ACGCCAGGGT TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGAATT CCAACCTTCC
  241 AATTCATTAA ATCGAGTCCT CCATCAGATG ATTTACAAGG TTTTGCATAG AATCCCTAAG
  301 TAAGAGAAGT TGTTAGTGAT TTCTTCAAGT TTACTAAAAT TTACATACAA ATGGATGATC
  361 TCTTCTCCAT TGTTTCCTTT CTTCTTGCAA ACGCGTTTTA CAAAGAGATG ACATGCTATT
  421 TTCTGATTAT TTTTTTCTAT AGTTTTCTAT TTTGTACGTC CTTGGGAGTC CGAAATGTAA
  481 AATCGGGGTA TGGTTAGTAG CGGCAGCGTT CATTATGGTA TATAAAAGAT AAAAAGTAGG
  541 GGCAAGCGAG ACAGAATGGG ATACAAGGGC ATCGTCTATA ATTATAGCTA AAAATTGTAT
  601 TTTAATTTGT ATTTTTTGTA ATTTTATTTC TCATTGTTTT ACTTAAAATG AATGCGAATT
  661 AGAGAAAACT TCAACGAAAT GTCAAAATAA GCTCAGCAAA ATATACAATT TTAGGGAAAG
  721 CGATCAGCAA CTTATTCTCC GTTGTAGATT CTTTTTCTTC ATAAATTCCA ACAATGTAAC
  781 TGCCAATTGA TTTTTCTCTA GATGCTAATA AATTGAAACT AATGAATATA ATAACACAGG
  841 TTTTGATAAA TTGTAAACTC TTACTCAAAA AAAAGAATGT TAAAACTAAT TTAACCCTTT
  901 TTTATAAAGG CTCATGGGCG TTTAAAGAGG GATAGTAAAA ATTTCTACAA TTTTTTTAAT
  961 TTAAATGTCA CAATTTGTCT TTGCGAATAT AATTCTATTA ATTTTCGCTA ACTGCTGTTT
 1021 TAGTACTATT TACTTTCCAT ATGCCTTATA TGCACTTTAA TCATGAGATG CATATTTTTG
1081 CATCTTCCTG TACTGGAACG CTTTTTTAAT CCAATTTAAG TAACA
//
Primer: LEU2pro_F
Sequence file: RDB14499_A6L7c.seq check
>06588_14499_A6L7_LEU2pro_F_E10_13_ABI24.ab1
    1 TATCATTTCA GTTGAAATAT TATTTAAGGA CCTATTGTTT TTTCCAATAG GTGGTTAGCA
   61 ATCGTCTTAC TTTCTAACTT TTCTTACCTT TTACATTTCA GCAATATATA TATATATTTC
  121 AAGGATATAC CATTCTAATG TCTGCCCCTA AGAAGATCGT CGTTTTGCCA GGTGACCACG
  181 TTGGTCAAGA AATCACAGCC GAAGCCATTA AGGTTCTTAA AGCTATTTCT GATGTTCGTT
  241 CCAATGTCAA GTTCGATTTC GAAAATCATT TAATTGGTGG TGCTGCTATC GATGCTACAG
  301 GTGTTCCACT TCCAGATGAG GCGCTGGAAG CCTCCAAGAA GGCTGATGCC GTTTTGTTAG
  361 GTGCTGTGGG TGGTCCTAAA TGGGGTACCG GTAGTGTTAG ACCTGAACAA GGTTTACTAA
  421 AAATCCGTAA AGAACTTCAA TTGTACGCCA ACTTAAGACC ATGTAACTTT GCATCCGACT
  481 CTCTTTTAGA CTTATCTCCA ATCAAGCCAC AATTTGCTAA AGGTACTGAC TTCGTTGTTG
  541 TCAGAGAATT AGTGGGAGGT ATTTACTTTG GTAAGAGAAA GGAAGACGAT GGTGATGGTG
  601 TCGCTTGGGA TAGTGAACAA TACACCGTTC CAGAAGTGCA AAGAATCACA AGAATGGCCG
  661 CTTTCATGGC CCTACAACAT GAGCCACCAT TGCCTATTTG GTCCTTGGAT AAAGCTAATG
  721 TTTTGGCCTC TTCAAGATTA TGGAGAAAAA CTGTGGAGGA AACCATCAAG AACGAATTCC
  781 CTACATTGAA GGTTCAACAT CAATTGATTG ATTCTGCCGC CATGATCCTA GTTAAGAACC
  841 CAACCCACCT AAATGGTATT ATAATCACCA GCAACATGTT TGGTGACATC ATCTCCGATG
  901 AAGCCCTCCG TTATCCCAGG TTCCTTGGGT TTGTTGCCAT CTGCGTCCTT GGCCCTCTTT
  961 GCCAGACAAG AACACCGCAT TGGGTTTGTA CGAAACCATG CCACGGTTCT GCTCCAGATT
 1021 TGCCAAAGAT AAAGTCAACC CTATCGCCAC TATCTGTCTG CCTGCAATGA TGATGAATGT
1081 CATGGACTTG CCTGGAAAGA AGGTAAGGGG TCCAAATGTG A
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Matsuyama, A., pDUAL, a multipurpose, multicopy vector capable of chromosomal integration in fission yeast. Yeast,.21 (15):1289-1305 (2004). PMID 15546162. [link to RRC of NBRP]