Please review the QC test results indicated by 
icon below as well as clone information before placing your order.
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Please review the QC test results indicated by icon below as well as clone information before placing your order.
icon below as well as clone information before placing your order.
pCMFlag_hsBIRC5 (#RDB06597)
Expression vector of human BIRC5/API4.
| Alternative name | SET-0187_2 |
|---|---|
| Clone info. | Expression vector of human BIRC5/API4. Expression was confirmed by Western blotting with anti-FLAG antibody. |
| Comment | Derived from Sugano KAT00573. PCR cloning, forward primer: ggtgccccgacgttgccccctg; reverse primer: tcaatccatggcagccagctgctcg. |
| Vector backbone | pCMV_S-FLAG (RDB05956) (Plasmid) |
| Size of vector backbone | 5.5 kb |
| Selectable markers | Amp^r (E. coli), Neo^r (mammalian cell) |
| Growth conditions | 37C, LB+Amp |
| Gene/insert name | Human BIRC5/API4 cDNA |
| Depositor|Developer | DNA Bank, | |
Distribution information
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Ordering forms | Order form [Credit Card Payment  ] [Bank Transfer Payment] [Example of order form ]MTA, for use for not-for-profit academic purpose [Word ] [Example of MTA ]MTA, for use for for-profit purpose [Word ]Please visit Information of Request for Distribution.[link] |
|---|---|
| Terms and conditions for distribution | The RECIPIENT agrees to describe RIKEN BRC as the source of the MATERIAL and cite Gene 200, 149-156, 1997 in any publication. Additional terms and conditions: Regrding pRc/CMV: a) The modified pRc/CMV, and their descendants (collectively, LIFE MATERIALS), should be used for pre-clinical/non-human, research use only. b) LIFE MATERIALS shall not be used for any commercial purposes. c) LIFE MATERIALS shall not be transferred beyond the specified recipient. d) Recipient must contact Thermo Fisher Scientific Inc. at outlicense@thermofisher.com for any desired use of LIFE MATERIALS beyond the scope of a) to c) above. |
[open/close]| 必要書類 | 提供依頼書 [依頼書の記入例 ]提供同意書 (MTA, 非営利学術目的用)[Word ] [MTAの記入例 ]提供同意書 (MTA, 営利目的用)[Word ]手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
|---|---|
| MTAに書く使用条件 | 発表に際し、理研BRCから入手したことを明記し、指定する文献(Gene, 200, 149-156 (1997))を引用すること。 付加的使用条件： pRc/CMVについて: a) The modified pRc/CMV, and their descendants (collectively, LIFE MATERIALS), should be used for pre-clinical/non-human, research use only. b) LIFE MATERIALS shall not be used for any commercial purposes. c) LIFE MATERIALS shall not be transferred beyond the specified recipient. d) Recipient must contact Thermo Fisher Scientific Inc. at outlicense@thermofisher.com for any desired use of LIFE MATERIALS beyond the scope of a) to c) above. |
| Catalog # | Resource name | Shipping form | Fee |
|---|---|---|---|
| RDB06597 | pCMFlag_hsBIRC5 | DNA solution |
JPY 9,460 (not-for-profit academic purpose) JPY 18,920 (for-profit-research purpose) plus cost of shipping containers, dry ice (if required) and shipping charge |
icon below as well as clone information before placing your order.How to cite this biological resource
Materials & Methods section:
| The pCMFlag_hsBIRC5 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06597). |
Reference section:
Further references such as user reports and related articles (go to bottom)
QC test results
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
| Test sheet | RDB12969_A4J1.pdf |
|---|
Nucleotide sequence of a portion of this resource (if available).
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Primer: CMV-Forward Sequence file: RDB12969_A4J1a.seq
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>06597_12969_A4J1_CMV-Foward_A03_03.ab1 1 TTGCTTCAAC TCCGGCCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC 61 TATATAAGCA GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT 121 AATACGACTC ACTATAGGGA GACCCAAGCT GAATTCACCA TGGACTACAA GGACGATGAC 181 GATAAGGCGG CCGGTGCCCC GACGTTGCCC CCTGCCTGGC AGCCCTTTCT CAAGGACCAC 241 CGCATCTCTA CATTCAAGAA CTGGCCCTTC TTGGAGGGCT GCGCCTGCAC CCCGGAGCGG 301 ATGGCCGAGG CTGGCTTCAT CCACTGCCCC ACTGAGAACG AGCCAGACTT GGCCCAGTGT 361 CTCTTCTGCT TCAAGGAGCT GGAAGGCTGG GAGCCAGATG ACGACCCCAT AGAGGAACAT 421 AAAAAGCATT CGTCCGGTTG CGCTTTCCTT TCTGTCAAGA AGCAGTTTGA AGAATTAACC 481 CTTGGTGAAT TTTTGAAACT GGACAGAGAA AGAGCCAAGA ACAAAATTGC AAAGGAAACC 541 AACAATAAGA AGAAAGAATT TGAGGAAACT GCGAAGAAAG TGCGCCGTGC CATCGAGCAG 601 CTGGCTGCCA TGGATTGAGG CCGCTCGAGC ATGCATCTAG AGGGCCCTAT TCTATAGTGT 661 CACCTAAATG CTAGAGCTCG CTGATCAGCC TCGACTGTGC CTTCTAGTTG CCAGCCATCT 721 GTTGTTTGCC CCTCCCCCGT GCCTTCCTTG ACCCTGGAAG GTGCCACTCC CACTGTCCTT 781 TCCTAATAAA ATGAGGAAAT TGCATCGCAT TGTCTGAGTA GGTGTCATTC TATTCTGGGG 841 GGTGGGGTGG GGCAGGACAG CAAGGGGGAG GATTGGGAAG ACAATAGCAG GCATGCTGGG 901 GATGCGGTGG GCTCTATGGC TTCTGAGGCG GAAAGAACCA GCTGGGGCTC GAGGGGGGAT 961 CCCCACGCGC CCTGTAGCGG CGCATTAAGC GCGGCCGGGT GTGGTGGTTA CGCGCAGCGT 1021 GAACCGCTAC ACTTGCCAGC GCCCTAGCGC CGCTCTTTCG CTTTTCTCCC GTCGTTTCTC 1081 GGCCACGGTT CGGCGCTTAC CCGGTTCAGG CTCTAATCCG GGGGCTCCCT TTGAGGGGAT 1141 TTCCCGA // |
Please visit 
Sequencing and PCR primers for primer information.
References
Original, user report and related articles
Resource search
Link
Other resources in our bankExternal Database
Human BIRC5/API4
Reference sequence
AK223428
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