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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCMFlag_hsBIRC5

Expression vector of human BIRC5/API4.

Catalog number RDB06597
Resource name pCMFlag_hsBIRC5
Alternative name SET-0187_2
Clone info. Expression vector of human BIRC5/API4. Expression was confirmed by Western blotting with anti-FLAG antibody.
Comment Derived from Sugano KAT00573. PCR cloning, forward primer: ggtgccccgacgttgccccctg; reverse primer: tcaatccatggcagccagctgctcg.
Vector backbone pCMV_S-FLAG (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human BIRC5/API4 cDNA
Depositor|Developer DNA Bank, |
Other clones in our bank
External Database
Human BIRC5/API4
          Reference sequence
            AK223428

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR The RECIPIENT agrees to describe RIKEN BRC as the source of the MATERIAL and cite Gene 200, 149-156, 1997 in any publication.
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          提供条件 発表に際し、理研BRCから入手したことを明記し、指定する文献(Gene, 200, 149-156 (1997))を引用すること。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
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          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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          RDB06597 pCMFlag_hsBIRC5 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content pCMV_tag and pRSV_tag assay-ready clones (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB12969_A4J1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV-Forward
          Sequence file: RDB12969_A4J1a.seq check
          >06597_12969_A4J1_CMV-Foward_A03_03.ab1
              1 TTGCTTCAAC TCCGGCCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC
             61 TATATAAGCA GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT
            121 AATACGACTC ACTATAGGGA GACCCAAGCT GAATTCACCA TGGACTACAA GGACGATGAC
            181 GATAAGGCGG CCGGTGCCCC GACGTTGCCC CCTGCCTGGC AGCCCTTTCT CAAGGACCAC
            241 CGCATCTCTA CATTCAAGAA CTGGCCCTTC TTGGAGGGCT GCGCCTGCAC CCCGGAGCGG
            301 ATGGCCGAGG CTGGCTTCAT CCACTGCCCC ACTGAGAACG AGCCAGACTT GGCCCAGTGT
            361 CTCTTCTGCT TCAAGGAGCT GGAAGGCTGG GAGCCAGATG ACGACCCCAT AGAGGAACAT
            421 AAAAAGCATT CGTCCGGTTG CGCTTTCCTT TCTGTCAAGA AGCAGTTTGA AGAATTAACC
            481 CTTGGTGAAT TTTTGAAACT GGACAGAGAA AGAGCCAAGA ACAAAATTGC AAAGGAAACC
            541 AACAATAAGA AGAAAGAATT TGAGGAAACT GCGAAGAAAG TGCGCCGTGC CATCGAGCAG
            601 CTGGCTGCCA TGGATTGAGG CCGCTCGAGC ATGCATCTAG AGGGCCCTAT TCTATAGTGT
            661 CACCTAAATG CTAGAGCTCG CTGATCAGCC TCGACTGTGC CTTCTAGTTG CCAGCCATCT
            721 GTTGTTTGCC CCTCCCCCGT GCCTTCCTTG ACCCTGGAAG GTGCCACTCC CACTGTCCTT
            781 TCCTAATAAA ATGAGGAAAT TGCATCGCAT TGTCTGAGTA GGTGTCATTC TATTCTGGGG
            841 GGTGGGGTGG GGCAGGACAG CAAGGGGGAG GATTGGGAAG ACAATAGCAG GCATGCTGGG
            901 GATGCGGTGG GCTCTATGGC TTCTGAGGCG GAAAGAACCA GCTGGGGCTC GAGGGGGGAT
            961 CCCCACGCGC CCTGTAGCGG CGCATTAAGC GCGGCCGGGT GTGGTGGTTA CGCGCAGCGT
           1021 GAACCGCTAC ACTTGCCAGC GCCCTAGCGC CGCTCTTTCG CTTTTCTCCC GTCGTTTCTC
           1081 GGCCACGGTT CGGCGCTTAC CCGGTTCAGG CTCTAATCCG GGGGCTCCCT TTGAGGGGAT
          1141 TTCCCGA
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          2022.05.18

          GNP_filter3_RDBDEP_html_220516.pl