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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCMFlag_hsOct3/4 isoform1

Expression vector of human Oct3/4 isofom 1.

Catalog number RDB06598
Resource name pCMFlag_hsOct3/4 isoform1
Alternative name SET-0212_14-1
Clone info. Expression vector of human Oct3/4 isofom 1. Expression was confirmed by Western blotting with anti-FLAG antibody.
Comment PCR cloning, forward primer: gcgggacacctggcttcggatt; reverse primer: cctcagtttgaatgcatgggaga. Nucleotide substitution C81T (Phe9Phe), C843T (Ile263Ile), C1101T (Ser349Ser) to NM_002701.
Vector backbone pCMV_S-FLAG (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human Oct3/4 cDNA
Depositor|Developer DNA Bank, |
Other clones in our bank
External Database
Human Oct3/4
          Reference sequence
            
           
          Remarks, protocol and/or map (pdf) RDB06598.pdf

          Distribution information

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          RDB06598 pCMFlag_hsOct3/4 isoform1 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pCMFlag_hsOct3/4 isoform1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06598).

          Reference section:

          Further references such as user reports and related articles (go to bottom)

          References and tips

          Featured content

          Featured content pCMV_tag and pRSV_tag assay-ready clones (English text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB12306_A3Jup1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward (Pr0016)
          Region: CMV pro,FLAG,insert 5'
          Sequence file: RDB12306_A3Jua.seq check
          >06598_12306_B1AjF_CMV-Forward_D10_10_ABI24.ab1
              1 NNNNNNNNNC NNNNNNNNNN NNGCNNATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA
             61 TAAGCAGAGC TCTCTGGCTA ACTAGAGAAC CCACTGCTTA CTGGCTTATC GAAATTAATA
            121 CGACTCACTA TAGGGAGACC CAAGCTGAAT TCACCATGGA CTACAAGGAC GATGACGATA
            181 AGGCGGCCGC GGGACACCTG GCTTCGGATT TTGCCTTCTC GCCCCCTCCA GGTGGTGGAG
            241 GTGATGGGCC AGGGGGGCCG GAGCCGGGCT GGGTTGATCC TCGGACCTGG CTAAGCTTCC
            301 AAGGCCCTCC TGGAGGGCCA GGAATCGGGC CGGGGGTTGG GCCAGGCTCT GAGGTGTGGG
            361 GGATTCCCCC ATGCCCCCCG CCGTATGAGT TCTGTGGGGG GATGGCGTAC TGTGGGCCCC
            421 AGGTTGGAGT GGGGCTAGTG CCCCAAGGCG GCTTGGAGAC CTCTCAGCCT GAGGGCGAAG
            481 CAGGAGTCGG GGTGGAGAGC AACTCCGATG GGGCCTCCCC GGAGCCCTGC ANNNNNNCCC
            541 CTGGTGCCGT GNAGCTGGAG AANGANAANC TGGAGCAAAA CCCNNNNGAN TCCCNNGACA
            601 TCNAAGCTCT GNNNAAANAN NNCGAGNNAT TTGNCNNGCT CCNNGAAGCN NAANANGNTN
            661 NNCCNGGGNN NNACANNNGN CGANGTGNGN NCNNNNNNNG GNTNNNTTNN GNANNNNNNN
          721 NNN
          //
          Primer: bGH_rev3 (Pr0885)
          Region: bGHpA,insert 3'
          Sequence file: RDB12306_A3Jub.seq check
          >06598_12306_B1AjF_bGH_rev3_D11_11_ABI24.ab1
              1 NNNNNNNNNN NNGGNNGNNG GNNNNNNACA GATGGCTGGC AACTAGAAGG CACAGTCGAG
             61 GCTGATCAGC GAGCTCTAGC ATTTAGGTGA CACTATAGAA TAGGGCCCTC TAGATGCATG
            121 CTCGAGCGGC CCCTCAGTTT GAATGCATGG GAGAGCCCAG AGTGGTGACA GAGACAGGGG
            181 GAAAGGCTTC CCCCTCAGGG AAAGGGACCG AGGAGTACAG TGCAGTGAAG TGAGGGCTCC
            241 CATAGCCTGG GGTACCAAAA TGGGGCCCTG GGGCCAGAGG AAAGGACACT GGTCCCCCTG
            301 AGAAAGGAGA CCCAGCAGCC TCAAAATCCT CTCGTTGTGC ATAGTCGCTG CTTGATCGCT
            361 TGCCCTTCTG GCGCCGGTTA CAGAACCACA CTCGGACCAC ATCCTTCTCG AGCCCAAGCT
            421 GCTGGGCAAT GTGGCTGATC TGCTGCAGTG TGGGTTTCGG GCACTGCAGG AACAAATTCT
            481 CCAGGTTGCC TCTCACTCGG TTCTCGATAC TGGTTCGCTT TCTCTTTCGG GCCTGCACGA
            541 GGGTTTCTGC TTTGCATATC TCCTGAAGAN TTTCATTGTT GTCAGCTTCC TCCNCCCACT
            601 TCTGCAGCAA GGGCCGCAGC TTACACATGT TCTTNNAGCT AANCTGCNNA NCCTCNAAGC
            661 GGCANNNGGN CGTTTGNCTG AATANCNTNC NNAANANAAN CCCCAGGGNN ANCCCNNCNT
          721 CNGNNNGNGN NNNNNCNNNN NNNNNN
          //
          Primer: SV40pro_ori_F (Pr0731)
          Region: SV40 pro_ori,NeoR
          Sequence file: RDB12306_A3Juc.seq check
          >06598_12306_B1AjF_SV40pro_ori_F_D12_12_ABI24.ab1
              1 NNNNNNNNNN NNNGCCGCCT CNGCCTCTGA GCTATTCNNG AAGTAGTGAG GAGGCTTTTT
             61 TGGAGGCCTA GGCTTTTGCA AAAAGCTCCC GGGAGCTTGG ATATCCATTT TCGGATCTGA
            121 TCAAGAGACA GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC
            181 TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG
            241 CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC
            301 CGACCTGTCC GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC
            361 CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG
            421 GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA
            481 GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG
            541 CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG
            601 TCTTGTCNAT CANGANGATC TGGACNAANA NCATCANGGG CTCGCGCCNG CCGAACTGTT
            661 CNNCAGGCTC ANNNNGCGCA NGNCCNANNN CNAGNANCNC NNCNNGNNCC NNGGNNNNGN
          721 CNGNTTGNNN ANNANNNNGN NNNNNANNNN NNNNTTNNNN NNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          user_report Lin, Y.C., Bovine induced pluripotent stem cells are more resistant to apoptosis than testicular cells in response to mono-(2-ethylhexyl) phthalate. Int. J. Mol. Sci., 15 (3): 5011-5031 (2014). PMID 24658443.

          2023.05.02

          GNP_filter3_RDBDEP_html_230501.pl