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pCMFlag_hsOct3/4 isoform1 (#RDB06598)

Expression vector of human Oct3/4 isofom 1.


Drawn by SnapGene® software
Sequence (full) RDB06598hts01.seq
Remarks, protocol and/or map (pdf) RDB06598.pdf
Publication Unpublished bioresource
Test sheet RDB12306_A7Bmp1-3.pdf 
Assembled from experimentally sequenced data.
 
Alternative name SET-0212_14-1
Clone info. Expression vector of human Oct3/4 isofom 1. Expression was confirmed by Western blotting with anti-FLAG antibody.
Comment PCR cloning, forward primer: gcgggacacctggcttcggatt; reverse primer: cctcagtttgaatgcatgggaga. Nucleotide substitution C89T (Phe9, syn), C851T (Ile263, syn), C1109T (Ser349, syn) to NM_002701.6.
Vector backbone pCMV_S-FLAG (RDB05956) (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Growth conditions 37C, LB+Amp
Gene/insert name Human Oct3/4 cDNA
Depositor|Developer DNA Bank, |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution The RECIPIENT agrees to describe RIKEN BRC as the source of the MATERIAL in any publication.
Additional terms and conditions:
Regrding pRc/CMV:
a) The modified pRc/CMV, and their descendants (collectively, LIFE MATERIALS), should be used for pre-clinical/non-human, research use only.
b) LIFE MATERIALS shall not be used for any commercial purposes.
c) LIFE MATERIALS shall not be transferred beyond the specified recipient.
d) Recipient must contact Thermo Fisher Scientific Inc. at outlicense@thermofisher.com for any desired use of LIFE MATERIALS beyond the scope of a) to c) above.
提供案内 (日本国内) [open/close]

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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
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MTAに書く使用条件 発表に際し、理研BRCから入手したことを明記すること。
付加的使用条件：
pRc/CMVについて:
a) The modified pRc/CMV, and their descendants (collectively, LIFE MATERIALS), should be used for pre-clinical/non-human, research use only.
b) LIFE MATERIALS shall not be used for any commercial purposes.
c) LIFE MATERIALS shall not be transferred beyond the specified recipient.
d) Recipient must contact Thermo Fisher Scientific Inc. at outlicense@thermofisher.com for any desired use of LIFE MATERIALS beyond the scope of a) to c) above.

Catalog # Resource name Shipping form Fee
RDB06598 pCMFlag_hsOct3/4 isoform1 DNA solution JPY 9,460 (not-for-profit academic purpose)
JPY 18,920 (for-profit-research purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pCMFlag_hsOct3/4 isoform1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06598).

Reference section:

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

user_report Lin, Y.C., Bovine induced pluripotent stem cells are more resistant to apoptosis than testicular cells in response to mono-(2-ethylhexyl) phthalate. Int. J. Mol. Sci., 15 (3): 5011-5031 (2014). PMID 24658443. [link to RRC of NBRP]