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pCMFlag_hsNFKBIA

Expression vector of human NFKBIA/IkappaB alpha.

Catalog number RDB06668
Resource name pCMFlag_hsNFKBIA
Alternative name SET-0206_4-1
Clone info. Expression vector of human NFKBIA/IkappaB alpha. Expression was confirmed by Western blotting with anti-FLAG antibody.
Comment Derived from Sugano DMC05321. PCR cloning, forward primer: atgttccaggcggccgagcg; reverse primer: tgcactcataacgtcagacgctg. Nucleotide substitution T400C (silent) to NM_020529.
Vector backbone pCMV_S-FLAG (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human NFKBIA/IkappaB alpha cDNA
Depositor|Developer DNA Bank, |
Other clones in our bank
External Database
Human NFKBIA/IkappaB alpha
          Reference sequence
            
           
          Remarks, protocol and/or map (pdf) RDB06668.pdf

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution The RECIPIENT agrees to describe RIKEN BRC as the source of the MATERIAL and cite Gene 200, 149-156, 1997 in any publication.
          Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          提供案内 (日本国内) [open/close]

          提供条件 発表に際し、理研BRCから入手したことを明記し、指定する文献(Gene, 200, 149-156 (1997))を引用すること。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Availability Shipping form Fee (non-profit org.)
          RDB06668 pCMFlag_hsNFKBIA Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          How to cite this biological resource

          Materials & Methods section:

          The pCMFlag_hsNFKBIA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06668).

          Reference section:

          Further references such as user reports and related articles (go to bottom)

          References and tips

          Featured content

          Featured content pCMV_tag and pRSV_tag assay-ready clones (English text)

          QC test results

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References

          Original, user report and related articles

          user_report Rahimova, N., Development of mKO2 fusion proteins for real-time imaging and mechanistic investigation of the degradation kinetics of human IkappaBalpha in living cells. Biochim. Biophys. Acta Mol. Cell Res. 1866 (2): 190-198 (2018). PMID 30391277.

          2023.05.02

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