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pCMFlag_hsINS

Expression vector of human INS.

Catalog number RDB07024
Resource name pCMFlag_hsINS
Alternative name SET-0203_12
Clone info. Expression vector of human INS. Expression has not yet been confirmed.
Comment Derived from Sugano PNC01554. PCR cloning, forward primer: gccctgtggatgcgcctcctgcc; reverse primer: ctagttgcagtagttctccagc.
Vector backbone pCMV_S-FLAG (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human INS cDNA
Depositor|Developer DNA Bank, |
Other clones in our bank
External Database
Human INS
          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR The RECIPIENT agrees to describe RIKEN BRC as the source of the MATERIAL and cite Gene 200, 149-156, 1997 in any publication.
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          提供条件 発表に際し、理研BRCから入手したことを明記し、指定する文献(Gene, 200, 149-156 (1997))を引用すること。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
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          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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          RDB07024 pCMFlag_hsINS DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB12835_A4Gvp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV-Forward
          Sequence file: RDB12835_A4Gva.seq check
          >07024_12835_A4Gv_CMV-Foward_C02_08.ab1
              1 CAGATTAAAA ACCCCCCGGC CCCCATTGAC GCAAATGGAC GGTAGGCGTG TACGGTGGGA
             61 GGTCTATATA AGCAGAGCTC TCTGGCTAAC TAGAGAACCC ACTGCTTACT GGCTTATCGA
            121 AATTAATACG ACTCACTATA GGGAGACCCA AGCTGAATTC ACCATGGACT ACAAGGACGA
            181 TGACGATAAG GCGGCCGCCC TGTGGATGCG CCTCCTGCCC CTGCTGGCGC TGCTGGCCCT
            241 CTGGGGACCT GACCCAGCCG CAGCCTTTGT GAACCAACAC CTGTGCGGCT CACACCTGGT
            301 GGAAGCTCTC TACCTAGTGT GCGGGGAACG AGGCTTCTTC TACACACCCA AGACCCGCCG
            361 GGAGGCAGAG GACCTGCAGG TGGGGCAGGT GGAGCTGGGC GGGGGCCCTG GTGCAGGCAG
            421 CCTGCAGCCC TTGGCCCTGG AGGGGTCCCT GCAGAAGCGT GGCATTGTGG AACAATGCTG
            481 TACCAGCATC TGCTCCCTCT ACCAGCTGGA GAACTACTGC AACTAGGGCC GCTCGAGCAT
            541 GCATCTAGAG GGCCCTATTC TATAGTGTCA CCTAAATGCT AGAGCTCGCT GATCAGCCTC
            601 GACTGTGCCT TCTAGTTGCC AGCCATCTGT TGTTTGCCCC TCCCCCGTGC CTTCCTTGAC
            661 CCTGGAAGGT GCCACTCCCA CTGTCCTTTC CTAATAAAAT GAGGAAATTG CATCGCATTG
            721 TCTGAGTAGG TGTCATTCTA TTCTGGGGGG TGGGGTGGGG CAGGACAGCA AGGGGGAGGA
            781 TTGGGAAGAC AATAGCAGGC ATGCTGGGGG ATGCGGTGGG CTCTATGGGC TTCTGAGGCG
            841 AAAAGAACCA GCTGGGGGGC TCGAGGGGGG ATCCCCCACG CGCCCTGTAG CGGCGCATTA
          901 AGCGCGGGCG GGGTGTGGGT GG
          //
          Primer: SV40pro_F_V2
          Sequence file: RDB12835_A4Gvb.seq check
          >07024_12835_A4Gv_SV40pro_F_V2_D04_10.ab1
              1 GTAGGAGGTT TTTTGGGAGG GCCTAGGCTT TTGCAAAAAG CTCCCGGGAG CTTGGATATC
             61 CATTTTCGGA TCTGATCAAG AGACAGGATG AGGATCGTTT CGCATGATTG AACAAGATGG
            121 ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA
            181 ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT
            241 TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA CTGCAGGACG AGGCAGCGCG
            301 GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA
            361 AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA
            421 CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA ATGCGGCGGC TGCATACGCT
            481 TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT CGCATCGAGC GAGCACGTAC
            541 TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC GAAGAGCATC AGGGGCTCGC
            601 GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GCGCATGCCC GACGGCGAGG ATCTCGTCGT
            661 GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA AAATGGCCGC TTTTCTGGAT
            721 TCATCGACTG TGGCCGGCTG GGTGTGGCGG ACCGCTATCA GGACATAGCG TTGGCTACCC
            781 GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGGCTGACC GCTTCCTCGT GCTTTACGGT
            841 ATCGCCGCTC CCGATTCGCA GCGCATCGCC CTTCTATCGC CTTCTTGACG AGTTCTTCTG
            901 AGCGGGACTC TGGGGTTCGA AATGACCGAC CAAGCGACGC CCAACCTGCC CATCACGAGA
            961 TTTTCGATTT CCACCCGCCG CCCTTCCTAT GAAAGGTTGG GCTTTCGGAA ATCGGTTTTC
           1021 GGACGTCCGG CCTGGAATGA TTCTCAGCGC GGGGATCTCA TGCTGCAAGC TTCCTTCGAC
          1081 CTAGCTCCGA ACCTTGTTA
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl