Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pSRR2 tk-luc (#RDB07095)

Oct-Sox enhancer carrying reporter derived from Sox2.

Alternative name pSox2-luc, stock no 1419
Clone info. Reporter construct with luciferase expression under the Sox2 3' enhancer.
Comment Use ptk-luc (RDB07031; stock no. 649) as a control.
Vector backbone ptk-luc (Plasmid)
Selectable markers Amp^r
Gene/insert name Mouse Sox2 genomic DNA
Depositor|Developer Niwa, Hitoshi |
 
Sequence RDB07095z.seq
Additional map

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Tomioka, M., Nuc. Acids Res., 30 (14), 3202-3213, 2002)
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する （Tomioka, M., Nuc. Acids Res., 30 (14), 3202-3213, 2002)。
MTAに書く付加的使用条件：
本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB07095 pSRR2 tk-luc DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pSRR2 tk-luc was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07095).

Reference section:

Tomioka, M., Nishimoto, M., Miyagi, S., Katayanagi, T., Fukui, N., Niwa, H., Muramatsu, M., Okuda, A., Identification of Sox-2 regulatory region which is under the control of Oct-3/4-Sox-2 complex. Nuc. Acids Res., 30 (14), 3202-3213, 2002. PMID 12136102. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB07095_A4E7.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F
Sequence file: RDB07095_A4E7a.seq check
>07095_07095_A4E7_1_pGL4-4174F_A02_02.ab1
    1 AGTAGGGTCA GACATTTCTC TATCGATAGG TACCGAGCTC TTACGCGTGC TAGCCCGGGC
   61 TCGAGATCCG GCCCCGCCCA GCGTCTTGTC ATTGGCGAAT TCGAACACGC AGATGCAGTC
  121 GGGGCGGCGC GGTCCGAGGT CCACTTCGCA TATTAAGGTG ACGCGTGTGG CCTCGAACAC
  181 CGAGCGACCC TGCAGCGACC CGCTTAACAG CGTCAACAGC GTGCCGCAGA TCTCGAAGCT
  241 TGGCATTCCG GTACTGTTGG TAAAGCCACC ATGGAAGACG CCAAAAACAT AAAGAAAGGC
  301 CCGGCGCCAT TCTATCCGCT GGAAGATGGA ACCGCTGGAG AGCAACTGCA TAAGGCTATG
  361 AAGAGATACG CCCTGGTTCC TGGAACAATT GCTTTTACAG ATGCACATAT CGAGGTGGAC
  421 ATCACTTACG CTGAGTACTT CGAAATGTCC GTTCGGTTGG CAGAAGCTAT GAAACGATAT
  481 GGGCTGAATA CAAATCACAG AATCGTCGTA TGCAGTGAAA ACTCTCTTCA ATTCTTTATG
  541 CCGGTGTTGG GCGCGTTATT TATCGGAGTT GCAGTTGCGC CCGCGAACGA CATTTATAAT
  601 GAACGTGAAT TGCTCAACAG TATGGGCATT TCGCAGCCTA CCGTGGTGTT CGTTTCCAAA
  661 AAGGGGTTGC AAAAAATTTT GAACGTGCAA AAAAAGCTCC CAATCATCCA AAAAATTATT
  721 ATCATGGATT CTAAAACGGA TTACCAGGGA TTTCAGTCGA TGTACACGTT CGTCACATCT
  781 CATCTACCTC CCGGTTTTAA TGAATACGAT TTTGTGCCAG AGTCCTTCGA TAGGGACAAG
  841 ACAATTGCAC TGATCATGAA CTCCTCTGGA TCTACTGGTC TGCCTAAAGG TGTCGCTCTG
  901 CCTCATAGAA CTGCCTGCGT GAGATTCTCG CATGCCAGAG ATCCTATTTT TGGCAATCAA
  961 ATCATTCCGG ATACTGCGAT TTTAAGTGTT GTTCCATTCC ATCACGGTTT TGGAATGTTT
 1021 ACTACACTCG GATATTTGAT ATGTGGATTT CGAGTCGTCT TTAATGTATA GATTTGGAAG
 1081 AGAGCTGTTT TCTGAGGAGC CTTTCATGAA TACAGGAATC AAGTGCGCTG CTGGATGCCA
1141 CCCCTAATTT CCTCCTTTTC TTTCCGGC
//
Primer: SV40pA3'Element Reverse
Sequence file: RDB07095_A4E7b.seq check
>07095_07095_A4E7_1_SV40pA3ElementReverse_D02_11.ab1
    1 GCGGTTCGGC GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC
   61 TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG
  121 ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC
  181 GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC
  241 ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG
  301 GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC TGTGCTCATT ACCACGTGAA
  361 TAATCCTATA TGCATCACAA TGGCTGCCCG AGCCCGGGAA ATTCTTTTAG AGTCTCTGCA
  421 TTAATTAGGG ATCCTTATCG ATTTTACCAC ATTTGTAGAG GTTTTACTTG CTTTAAAAAA
  481 CCTCCCACAC CTCCCCCTGA ACCTGAAACA TAAAATGAAT GCAATTGTTG TTGTTAACTT
  541 GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA
  601 AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTATCA
  661 TGTCTGCTCG AAGCGGCCGG CCGCCCCGAC TCTAGAATTA CACGGCGATC TTTCCGCCCT
  721 TCTTGGCCTT TATGAGGATC TCTCTGATTT TTCTTGCGTC GAGTTTTCCG GTAAGACCTT
  781 TCGGTACTTC GTCCACAAAC ACAACTCCTC CGCGCAACTT TTTCGCGGTT GTTACTTGAC
  841 TGGCGACGTA ATCCACGATC TCTTTTTCCG TCATCGTCTT TCCGTGCTCC AAACAACAAC
  901 GGCGGCGGGA AGTTCACCGG CGTCATCGTC GGGAAGACCT GCGACACCTG CGTCGAAGAT
  961 GTTGGGGGTG TTGGAGCAAG ATGGATTCCA ATTTCAGCGG GAGCCACCTG ATAGCCCTTT
 1021 GTACTTTAAT CAGAGGACTT CAGGCGGGTC CACGATGAGA AGTGTTTCGT CTTTCCGTCC
 1081 CAGTAAGCTA TGTCCTCCAG CATGTAGTCA TTCATCTTGT CAATCAAGCC GTTGGTCGCC
1141 TTCCGGGGAA TGTG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Tomioka, M., Identification of Sox-2 regulatory region which is under the control of Oct-3/4-Sox-2 complex. Nuc. Acids Res., 30 (14), 3202-3213, 2002. PMID 12136102. [link to RRC of NBRP]
reference Masui, S., Pluripotency governed by Sox2 via regulation of Oct3/4 expression in mouse embryonic stem cells. Nat. Cell Biol., 9, 625-635 (2007). PMID 17515932. [link to RRC of NBRP]